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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
15/11/2015 |
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Actualizado : |
31/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OLIVERA-MUZANTE, J.; GIL, J.; VIÑOLES, C.; FIERRO, S. |
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Afiliación : |
CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Reproductive outcome with GnRH inclusion at 24 or 36h following a prostaglandin F2α-based protocol for timed AI in ewes. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Animal Reproduction Science, 2013, v.138, no.3-4, p. 175-179. |
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ISSN : |
0378-4320 |
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DOI : |
10.1016/j.anireprosci.2013.02.013 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 2 October 2012 / Received in revised form 19 February 2013 / Accepted 21 February 2013 / Available online 28 February 2013. |
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Contenido : |
ABSTRACT.
The objective of this experiment was to study the reproductive performance obtained after a short-interval prostaglandin (PG) F2α-based protocol for timed artificial insemination (TAI) in sheep (Synchrovine®: two injections of PG 7 d apart), including a GnRH analogue at 24 or 36h after the second PG injection. The experiment involved 296 Corriedale ewes (206 multiparous and 90 nulliparous) grazing natural pastures during the breeding season (March-April; UTU " La Carolina" , Flores Uruguay, 33° S-57° W). Ewes were assigned to three treatment groups: a) Synchrovine® (Control, n=101): two injections of D-Cloprostenol 75μg, 7 d apart, b) Synchrovine®+GnRH24 (n=98): Synchrovine® plus GnRH (busereline acetate 8.4μg) 24h after the second PG injection, and c) Synchrovine®+GnRH36 (n=97): Synchrovine® plus GnRH 36h after the second PG injection. All ewes were subjected to cervical TAI (Day 0), 44 to 47h after second PG injection, with fresh extended semen pool from six rams. Reproductive performance of ewes having ovulations and ovulation rate on Day 10, estrous cycle length in ewes that returned to estrus and non-return rate to estrus up to Day 22, fertility, prolificacy and fecundity on Day 70 were analyzed. Ewes having ovulations, ovulation rate, estrous cycle length and prolificacy did not differ between groups (P>0.05). However, non-return to estrus, fertility and fecundity was decreased in Synchrovine®+GnRH24 (P<0.05) and similar between Synchrovine® and Synchrovine®+GnRH36 (P>0.05). It was concluded that the reproductive performance obtained by Synchovine® TAI protocol was impaired by GnRH at 24h and not improved by GnRH administered at 36h after the second PG injection.
© 2013 Elsevier B.V. MenosABSTRACT.
The objective of this experiment was to study the reproductive performance obtained after a short-interval prostaglandin (PG) F2α-based protocol for timed artificial insemination (TAI) in sheep (Synchrovine®: two injections of PG 7 d apart), including a GnRH analogue at 24 or 36h after the second PG injection. The experiment involved 296 Corriedale ewes (206 multiparous and 90 nulliparous) grazing natural pastures during the breeding season (March-April; UTU " La Carolina" , Flores Uruguay, 33° S-57° W). Ewes were assigned to three treatment groups: a) Synchrovine® (Control, n=101): two injections of D-Cloprostenol 75μg, 7 d apart, b) Synchrovine®+GnRH24 (n=98): Synchrovine® plus GnRH (busereline acetate 8.4μg) 24h after the second PG injection, and c) Synchrovine®+GnRH36 (n=97): Synchrovine® plus GnRH 36h after the second PG injection. All ewes were subjected to cervical TAI (Day 0), 44 to 47h after second PG injection, with fresh extended semen pool from six rams. Reproductive performance of ewes having ovulations and ovulation rate on Day 10, estrous cycle length in ewes that returned to estrus and non-return rate to estrus up to Day 22, fertility, prolificacy and fecundity on Day 70 were analyzed. Ewes having ovulations, ovulation rate, estrous cycle length and prolificacy did not differ between groups (P>0.05). However, non-return to estrus, fertility and fecundity was decreased in Synchrovine®+GnRH24 (P<0.05) and similar between Synchrovine® a... Presentar Todo |
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Palabras claves : |
Ewe; Fertility; Fixed timed artificial insemination; GnRH; Ovulation rate; Prostaglandin. |
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Thesagro : |
FERTILIDAD; OVEJA; OVINOS; OVULACION. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02803naa a2200313 a 4500
001 1053896
005 2019-10-31
008 2013 bl uuuu u00u1 u #d
022 $a0378-4320
024 7 $a10.1016/j.anireprosci.2013.02.013$2DOI
100 1 $aOLIVERA-MUZANTE, J.
245 $aReproductive outcome with GnRH inclusion at 24 or 36h following a prostaglandin F2α-based protocol for timed AI in ewes.$h[electronic resource]
260 $c2013
500 $aArticle history: Received 2 October 2012 / Received in revised form 19 February 2013 / Accepted 21 February 2013 / Available online 28 February 2013.
520 $aABSTRACT. The objective of this experiment was to study the reproductive performance obtained after a short-interval prostaglandin (PG) F2α-based protocol for timed artificial insemination (TAI) in sheep (Synchrovine®: two injections of PG 7 d apart), including a GnRH analogue at 24 or 36h after the second PG injection. The experiment involved 296 Corriedale ewes (206 multiparous and 90 nulliparous) grazing natural pastures during the breeding season (March-April; UTU " La Carolina" , Flores Uruguay, 33° S-57° W). Ewes were assigned to three treatment groups: a) Synchrovine® (Control, n=101): two injections of D-Cloprostenol 75μg, 7 d apart, b) Synchrovine®+GnRH24 (n=98): Synchrovine® plus GnRH (busereline acetate 8.4μg) 24h after the second PG injection, and c) Synchrovine®+GnRH36 (n=97): Synchrovine® plus GnRH 36h after the second PG injection. All ewes were subjected to cervical TAI (Day 0), 44 to 47h after second PG injection, with fresh extended semen pool from six rams. Reproductive performance of ewes having ovulations and ovulation rate on Day 10, estrous cycle length in ewes that returned to estrus and non-return rate to estrus up to Day 22, fertility, prolificacy and fecundity on Day 70 were analyzed. Ewes having ovulations, ovulation rate, estrous cycle length and prolificacy did not differ between groups (P>0.05). However, non-return to estrus, fertility and fecundity was decreased in Synchrovine®+GnRH24 (P<0.05) and similar between Synchrovine® and Synchrovine®+GnRH36 (P>0.05). It was concluded that the reproductive performance obtained by Synchovine® TAI protocol was impaired by GnRH at 24h and not improved by GnRH administered at 36h after the second PG injection. © 2013 Elsevier B.V.
650 $aFERTILIDAD
650 $aOVEJA
650 $aOVINOS
650 $aOVULACION
653 $aEwe
653 $aFertility
653 $aFixed timed artificial insemination
653 $aGnRH
653 $aOvulation rate
653 $aProstaglandin
700 1 $aGIL, J.
700 1 $aVIÑOLES, C.
700 1 $aFIERRO, S.
773 $tAnimal Reproduction Science, 2013$gv.138, no.3-4, p. 175-179.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
07/10/2014 |
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Actualizado : |
05/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
ALARCÓN-REVERTE, R.; GARCIA, M.A.; WATSON, S.B.; ABDALLAH, I.; SABATÉ, S.; HERNÁNDEZ, M.J.; DAYAN ,F.E.; FISCHER, A.J. |
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Afiliación : |
MILTON ALEJANDRO GARCIA LATASA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Pest Management Science, v. 71, n. 7, p. 996-2007, 2015. |
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ISSN : |
1526-4998. |
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DOI : |
10.1002/ps.3878 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 |
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Contenido : |
Abstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different populations may carry different resistance mechanisms and different mechanisms can
act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant
expression of EPSPS sensitivity to glyphosate. MenosAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different popula... Presentar Todo |
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Palabras claves : |
5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; EPSPS EXPRESSION; EPSPS INHIBITION ASSAY; POLYPLOIDY; RESISTANCE MECHANISM. |
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Thesagro : |
RESISTENCIA A GLIFOSATO. |
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Asunto categoría : |
H60 Malezas y escardas |
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Marc : |
LEADER 02883naa a2200313 a 4500
001 1050961
005 2019-11-05
008 2015 bl uuuu u00u1 u #d
022 $a1526-4998.
024 7 $a10.1002/ps.3878$2DOI
100 1 $aALARCÓN-REVERTE, R.
245 $aConcerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California.$h[electronic resource]
260 $c2015
500 $aArticle history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014
520 $aAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide, was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance. RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption, translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants. Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants. CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same population, individuals from different populations may carry different resistance mechanisms and different mechanisms can act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant expression of EPSPS sensitivity to glyphosate.
650 $aRESISTENCIA A GLIFOSATO
653 $a5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE
653 $aEPSPS EXPRESSION
653 $aEPSPS INHIBITION ASSAY
653 $aPOLYPLOIDY
653 $aRESISTANCE MECHANISM
700 1 $aGARCIA, M.A.
700 1 $aWATSON, S.B.
700 1 $aABDALLAH, I.
700 1 $aSABATÉ, S.
700 1 $aHERNÁNDEZ, M.J.
700 1 $aDAYAN ,F.E.
700 1 $aFISCHER, A.J.
773 $tPest Management Science$gv. 71, n. 7, p. 996-2007, 2015.
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