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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
02/01/2017 |
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Actualizado : |
02/01/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FIERRO, S.; VIÑOLES, C.; OLIVERA-MUZANTE, J. |
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Afiliación : |
S. FIERRO, SUL (Secretariado Uruguayo de la Lana).; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; J. OLIVERA-MUZANTE, Universidad de la República (UdelaR)/ Centro Universitario Regional Noroeste, Salto (CENUR). |
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Título : |
Long term prostaglandin based-protocols improve the reproductive performance after timed artificial insemination in sheep. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Theriogenology, 2017, v. 90, p. 109-113. |
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DOI : |
10.1016/j.theriogenology.2016.11.031 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 April 2016; Received in revised form; 28 September 2016; Accepted 30 November 2016; Available online 2 December 2016 |
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Contenido : |
ABSTRACT.
The aim of the experiment was to evaluate the reproductive performance of ewes synchronized with two doses of prostaglandin F2a (PG) at different intervals and inseminated at a fixed time. During the breeding season (April to June), 370 multiparous Corriedale ewes were assigned to five groups according to body condition score and body weight, and synchronized with two doses of PG administered 7, 10, 12, 14 or 16 days apart (groups PG7, PG10, PG12, PG14 or PG16; n ¼ 73, 76, 74, 72, 75; respectively). Cervical timed artificial insemination (Day 0) was performed at 48 ± 1.0 h (group PG7) or 56 ± 1.0 h (groups PG10, PG12, PG14 and PG16) after the second PG injection, with diluted fresh semen pooled from six adult rams. The percentage of ovulating ewes after the second PG injection and the ovulation rate (number of corpus luteum/ovulating ewes) were assessed on Day 10 by trans-rectal ultrasonography. The rate of non return to service (ewes not returning to service/inseminated ewes 100; NRR-21) was evaluated using painted vasectomized rams. Pregnancy rate (pregnant ewes/inseminated ewes 100) and prolificacy (foetuses/pregnant ewes) were determined on Day 60 by trans-abdominal ultrasonography. Higher NRR-21 and pregnancy rates was observed in groups PG12 (46.0%, 46.0%), PG14 (59.7%, 56.9%) and PG16 (58.7%, 56.0%) compared to PG7 (30.1%, 28.8%) and PG10 (30.3%, 30.3%; respectively P < 0.05). No significant differences were observed in the percentage of ovulating ewes, ovulation rate and prolificacy among groups (P > 0.05). Under the condition of this trial, 12, 14 or 16 days interval between PG injections enhances the pregnancy rate of ewes at cervical timed artificial insemination with fresh semen.
© 2016 Elsevier Inc. All rights reserved. MenosABSTRACT.
The aim of the experiment was to evaluate the reproductive performance of ewes synchronized with two doses of prostaglandin F2a (PG) at different intervals and inseminated at a fixed time. During the breeding season (April to June), 370 multiparous Corriedale ewes were assigned to five groups according to body condition score and body weight, and synchronized with two doses of PG administered 7, 10, 12, 14 or 16 days apart (groups PG7, PG10, PG12, PG14 or PG16; n ¼ 73, 76, 74, 72, 75; respectively). Cervical timed artificial insemination (Day 0) was performed at 48 ± 1.0 h (group PG7) or 56 ± 1.0 h (groups PG10, PG12, PG14 and PG16) after the second PG injection, with diluted fresh semen pooled from six adult rams. The percentage of ovulating ewes after the second PG injection and the ovulation rate (number of corpus luteum/ovulating ewes) were assessed on Day 10 by trans-rectal ultrasonography. The rate of non return to service (ewes not returning to service/inseminated ewes 100; NRR-21) was evaluated using painted vasectomized rams. Pregnancy rate (pregnant ewes/inseminated ewes 100) and prolificacy (foetuses/pregnant ewes) were determined on Day 60 by trans-abdominal ultrasonography. Higher NRR-21 and pregnancy rates was observed in groups PG12 (46.0%, 46.0%), PG14 (59.7%, 56.9%) and PG16 (58.7%, 56.0%) compared to PG7 (30.1%, 28.8%) and PG10 (30.3%, 30.3%; respectively P < 0.05). No significant differences were observed in the percentage of ovulating ewes, o... Presentar Todo |
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Palabras claves : |
EWE; FERTILITY; OESTRUS SYNCHRONIZATION; OVULATION RATE; PROTAGLANDIN; TIMED ARTIFICAL INSEMINATION. |
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Thesagro : |
OVEJA; OVINOS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02707naa a2200265 a 4500
001 1056404
005 2017-01-02
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2016.11.031$2DOI
100 1 $aFIERRO, S.
245 $aLong term prostaglandin based-protocols improve the reproductive performance after timed artificial insemination in sheep.$h[electronic resource]
260 $c2017
500 $aArticle history: Received 25 April 2016; Received in revised form; 28 September 2016; Accepted 30 November 2016; Available online 2 December 2016
520 $aABSTRACT. The aim of the experiment was to evaluate the reproductive performance of ewes synchronized with two doses of prostaglandin F2a (PG) at different intervals and inseminated at a fixed time. During the breeding season (April to June), 370 multiparous Corriedale ewes were assigned to five groups according to body condition score and body weight, and synchronized with two doses of PG administered 7, 10, 12, 14 or 16 days apart (groups PG7, PG10, PG12, PG14 or PG16; n ¼ 73, 76, 74, 72, 75; respectively). Cervical timed artificial insemination (Day 0) was performed at 48 ± 1.0 h (group PG7) or 56 ± 1.0 h (groups PG10, PG12, PG14 and PG16) after the second PG injection, with diluted fresh semen pooled from six adult rams. The percentage of ovulating ewes after the second PG injection and the ovulation rate (number of corpus luteum/ovulating ewes) were assessed on Day 10 by trans-rectal ultrasonography. The rate of non return to service (ewes not returning to service/inseminated ewes 100; NRR-21) was evaluated using painted vasectomized rams. Pregnancy rate (pregnant ewes/inseminated ewes 100) and prolificacy (foetuses/pregnant ewes) were determined on Day 60 by trans-abdominal ultrasonography. Higher NRR-21 and pregnancy rates was observed in groups PG12 (46.0%, 46.0%), PG14 (59.7%, 56.9%) and PG16 (58.7%, 56.0%) compared to PG7 (30.1%, 28.8%) and PG10 (30.3%, 30.3%; respectively P < 0.05). No significant differences were observed in the percentage of ovulating ewes, ovulation rate and prolificacy among groups (P > 0.05). Under the condition of this trial, 12, 14 or 16 days interval between PG injections enhances the pregnancy rate of ewes at cervical timed artificial insemination with fresh semen. © 2016 Elsevier Inc. All rights reserved.
650 $aOVEJA
650 $aOVINOS
653 $aEWE
653 $aFERTILITY
653 $aOESTRUS SYNCHRONIZATION
653 $aOVULATION RATE
653 $aPROTAGLANDIN
653 $aTIMED ARTIFICAL INSEMINATION
700 1 $aVIÑOLES, C.
700 1 $aOLIVERA-MUZANTE, J.
773 $tTheriogenology, 2017$gv. 90, p. 109-113.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
29/10/2020 |
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Actualizado : |
27/01/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GIANNITTI, F.; GARCÍA, J.P.; ROOD, J.I.; ADAMS, V.; ARMENDANO, J.I.; BEINGESSER, J.; UZAL, F.A. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE P. GARCÍA, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Buenos Aires, Argentina.; JULIAN I. ROOD, Monash University, Clayton, Victoria, Australia.; VICKI ADAMS, Monash University, Clayton, Victoria, Australia; JOAQUÍN I. ARMENDANO, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Buenos Aires, Argentina.; JULIANN BEINGESSER, University of California at Davis, San Bernardino, CA, USA.; FRANCISCO A. UZAL, University of California at Davis, San Bernardino, CA, USA. |
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Título : |
Cardiopulmonary Lesions in Sheep Produced by Experimental Acute Clostridium Perfringens Type D Enterotoxemia. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Veterinay Pathology, volumen 58, number 1, pag. 103-113, 2021. Doi: https://doi.org/10.1177/0300985820965554 |
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DOI : |
10.1177/0300985820965554 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Published October 15, 2020. Corresponding Author:Francisco A. Uzal, Email: fuzal@cahfs.ucdavis.edu. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Federico Giannitti acknowledges support from the Uruguayan ?Agencia Nacional de Investigaci ´on e Innovaci ´on? (ANII) through mobility grant MOV_CA_2018_1_150021. This work was supported by Grant R01 AI056177 from the National Institute of Allergy and Infectious Diseases (NIAID). Research at Monash University was also supported by funding provided by the Australian Research Council to the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics (Grant CE0562063). |
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Contenido : |
Abstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of
the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture
medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute cardiopulmonary lesions in sheep with experimental enterotoxemia are associated with the capacity of the strains to produce ETX. These changes are likely contributors to the clinical signs and even death of affected animals. MenosAbstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of
the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture
medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute ... Presentar Todo |
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Palabras claves : |
CLOSTRIDIUM PERFRINGENS TYPE D; EPSILON TOXIN; HEART; LUNGS; PATHOLOGY; PLATAFORMA SALUD ANIMAL; SHEEP. |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES; OVEJA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03466naa a2200325 a 4500
001 1061439
005 2021-01-27
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1177/0300985820965554$2DOI
100 1 $aGIANNITTI, F.
245 $aCardiopulmonary Lesions in Sheep Produced by Experimental Acute Clostridium Perfringens Type D Enterotoxemia.$h[electronic resource]
260 $c2021
500 $aArticle history: First Published October 15, 2020. Corresponding Author:Francisco A. Uzal, Email: fuzal@cahfs.ucdavis.edu. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Federico Giannitti acknowledges support from the Uruguayan ?Agencia Nacional de Investigaci ´on e Innovaci ´on? (ANII) through mobility grant MOV_CA_2018_1_150021. This work was supported by Grant R01 AI056177 from the National Institute of Allergy and Infectious Diseases (NIAID). Research at Monash University was also supported by funding provided by the Australian Research Council to the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics (Grant CE0562063).
520 $aAbstract:Enterotoxemia caused by Clostridium perfringens type D is one of the most prevalent clostridial diseases of sheep. The lesions of the acute form of this disease, particularly the cerebral lesions, are well characterized; however, detailed descriptions of the cardiac and pulmonary lesions are lacking. Here we describe cardiopulmonary lesions in experimental acute type D enterotoxemia in sheep and determine the role of epsilon toxin (ETX) in the development of these lesions. Four groups of 6 sheep were intraduodenally inoculated with either a wild-type C. perfringens type D strain; its etx knockout mutant, which is unable to produce ETX; the etx mutant complemented with the wild-type etx gene, which regains the ETX toxigenic ability; or sterile culture medium as a control. All sheep were subjected to postmortem examination within 24 hours of inoculation. Lesion scores were compared between groups for pulmonary edema; hydrothorax; ascites; hydropericardium; endocardial, myocardial and epicardial hemorrhages; microscopic lesions of acute myocardial degeneration and necrosis; and myocardial, endocardial, and epicardial edema, hemorrhage, and inflammation. Only sheep inoculated with the wild-type and complemented ETX-toxigenic bacterial strains developed cardiopulmonary lesions, which were present in varying degrees of severity and proportions. These lesions were not present in sheep inoculated with the etx mutant or in the negative control. We conclude that severe acute cardiopulmonary lesions in sheep with experimental enterotoxemia are associated with the capacity of the strains to produce ETX. These changes are likely contributors to the clinical signs and even death of affected animals.
650 $aENFERMEDADES DE LOS ANIMALES
650 $aOVEJA
653 $aCLOSTRIDIUM PERFRINGENS TYPE D
653 $aEPSILON TOXIN
653 $aHEART
653 $aLUNGS
653 $aPATHOLOGY
653 $aPLATAFORMA SALUD ANIMAL
653 $aSHEEP
700 1 $aGARCÍA, J.P.
700 1 $aROOD, J.I.
700 1 $aADAMS, V.
700 1 $aARMENDANO, J.I.
700 1 $aBEINGESSER, J.
700 1 $aUZAL, F.A.
773 $tVeterinay Pathology, volumen 58, number 1, pag. 103-113, 2021. Doi: https://doi.org/10.1177/0300985820965554
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