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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
08/03/2023 |
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Actualizado : |
08/03/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
COLOMBATTI OLIVIERI, M. A.; FRESIA, P.; GRAÑA, M.; CUERDA, M.X.; NAGEL, A.; ALVARADO PINEDO, F.; ROMANO, M. I.; CAIMI, K.; BERNÁ, L.; SANTANGELO, M. P. |
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Afiliación : |
M.A. COLOMBATTI OLIVIERI, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTACONICET, Dr. Nicolas ´ Repetto y De Los Reseros S/Nº B1686IGC, Hurlingham, Buenos Aires, Argentina; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; M. GRAÑA, Unidad de Bioinformatica, Institut Pasteur de Montevideo, Mataojo 2020, CP11400, Montevideo, Uruguay; M. X. CUERDA, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTACONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Hurlingham, Buenos Aires, Argentina; A. NAGEL, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTACONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Hurlingham, Buenos Aires, Argentina; F. ALVARADO PINEDO, Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE), Facultad de Ciencias Veterinarias - Universidad de La Plata (UNLP), Buenos Aires, Chascomus, Argentina; M. I. ROMANO, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina; K. CAIMI, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina; L. BERNÁ, Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, 11400, CP, Uruguay; M. P. SANTANGELO, Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina. |
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Título : |
Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype. |
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Complemento del título : |
Letter. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Tuberculosis, 2023, volume 138, article 102299. doi: https://doi.org/10.1016/j.tube.2022.102299 |
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ISSN : |
1472-9792 |
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DOI : |
10.1016/j.tube.2022.102299 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 September 2022; Received in revised form 28 November 2022; Accepted 19 December 2022; Available online 21 December 2022. -- Correspondence authors: Berná, L.; Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, CP, Uruguay, email:lberna@pasteur.edu.uy ; Santangelo, M.P.; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina, email:santangelo.maria@inta.gob.ar -- FUNDING: This work was supported by the National Institute of Agriculture Research (grant number PNSA PDI105); National Agency for Science and Technology Promotion, Argentina (grant number PICT 2016-0287); CONICET-Institut Pasteur Bilateral Cooperation Program. -- |
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Contenido : |
In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models. © 2022 Elsevier Ltd |
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Palabras claves : |
Genomics; Oxidative stress; Phenotype; Virulence; WGS. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 03150naa a2200325 a 4500
001 1063970
005 2023-03-08
008 2023 bl uuuu u00u1 u #d
022 $a1472-9792
024 7 $a10.1016/j.tube.2022.102299$2DOI
100 1 $aCOLOMBATTI OLIVIERI, M. A.
245 $aGenomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 30 September 2022; Received in revised form 28 November 2022; Accepted 19 December 2022; Available online 21 December 2022. -- Correspondence authors: Berná, L.; Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, CP, Uruguay, email:lberna@pasteur.edu.uy ; Santangelo, M.P.; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA-CONICET, Dr. Nicolás Repetto y De Los Reseros S/Nº B1686IGC, Buenos Aires, Hurlingham, Argentina, email:santangelo.maria@inta.gob.ar -- FUNDING: This work was supported by the National Institute of Agriculture Research (grant number PNSA PDI105); National Agency for Science and Technology Promotion, Argentina (grant number PICT 2016-0287); CONICET-Institut Pasteur Bilateral Cooperation Program. --
520 $aIn a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models. © 2022 Elsevier Ltd
653 $aGenomics
653 $aOxidative stress
653 $aPhenotype
653 $aVirulence
653 $aWGS
700 1 $aFRESIA, P.
700 1 $aGRAÑA, M.
700 1 $aCUERDA, M.X.
700 1 $aNAGEL, A.
700 1 $aALVARADO PINEDO, F.
700 1 $aROMANO, M. I.
700 1 $aCAIMI, K.
700 1 $aBERNÁ, L.
700 1 $aSANTANGELO, M. P.
773 $tTuberculosis, 2023, volume 138, article 102299. doi: https://doi.org/10.1016/j.tube.2022.102299
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
29/10/2019 |
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Actualizado : |
12/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
URRABURU, M.; AGUAYO, E.; ZOPPOLO, R.; SILVEIRA, A.C. |
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Afiliación : |
MARIANA URRABURU BORDON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. AGUAYO, Grupo de Postrecoleccio?n y Refrigeracio?n, Universidad Polite?cnica de Cartagena, Spain; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A.C. SILVEIRA, Poscosecha de Frutas y Hortalizas, Departamento de Produccio?n Vegetal, Facultad de Agronom??a, Universidad de La Repu?blica, Uruguay. |
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Título : |
Postharvest and functional behavior of some feijoa genetic materials. (Conference paper). |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Acta Horticulturae, 2018, no. 1194, pages 73-78. |
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Serie : |
(Acta Horticulturae; 1194) |
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ISBN : |
978-94-62611-90-0 |
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ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2018.1194.12 |
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Idioma : |
Inglés |
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Notas : |
In: Acta Horticulturae (ISHS) 1194: VIII International Postharvest Symposium: Enhancing Supply Chain and Consumer Benefits - Ethical and Technological Issues. Editors: F. Artés-Hernández, P.A. Gómez, E. Aguayo, F. Artés. Publication date: 4 april 2018. |
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Contenido : |
ABSTRACT.
Feijoa (Acca sellowiana (Berg.) Burret) is one of the most popular native Uruguayan trees whose functional characteristics have attracted the interest for commercial cultivation. The aim of this work was to evaluate the postharvest behavior of six genetic materials, identified as 95, 97, 98, 152, 153 and 154 from the Facultad de Agronomía and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) breeding program. Fruits harvested at two maturity stages (M1: not easily picked; M2: touch picking) were stored for 0, 15 and 30 days at 5°C and 95% relative humidity (RH) plus 7 days at 18°C (shelf life). Respiration rates and ethylene emissions were measured every 3 days, while flesh firmness, weight loss and total polyphenol content were evaluated at the end of the storage period and during shelf life. The respiration rate of all the genetic materials was around 27.6 mg kg-1 h-1 of CO2 during the storage period, while ethylene emission was maintained below 100 µL kg-1 h-1 without differences among maturity stages and genetic materials. Fruits harvested at the M1 stage were 40-60% more firm than fruits harvested at the M2 stage. Genetic materials identified as 95 and 98 had the highest firmness results, with values of 150.1 and 145.1 N (M1), 112.6 and 90.4 N (M2) at harvest and 28.8 and 17.4 N (M1) and 17.2 and 15.4 N (M2) after 30 days at 5°C + 7 days at 20°C, respectively. Average weight loss at the end of storage (30+7) was 14.7%. Fruits identified as material 152 had the highest polyphenol content with values at harvest of 2,384.5 mg GAE 100 g-1 DW (M1) and 2,130.7 mg GAE 100 g-1 DW (M2). At the end of the storage period (30+7), total polyphenol contents decreased between 30-70% in the M1 stage and between 20-50% in the M2 stage. Materials 95, 98 and 152 are interesting for commercial production due to their firmness retention and their functional composition.
© 2018 International Society for Horticultural Science. All rights reserved MenosABSTRACT.
Feijoa (Acca sellowiana (Berg.) Burret) is one of the most popular native Uruguayan trees whose functional characteristics have attracted the interest for commercial cultivation. The aim of this work was to evaluate the postharvest behavior of six genetic materials, identified as 95, 97, 98, 152, 153 and 154 from the Facultad de Agronomía and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) breeding program. Fruits harvested at two maturity stages (M1: not easily picked; M2: touch picking) were stored for 0, 15 and 30 days at 5°C and 95% relative humidity (RH) plus 7 days at 18°C (shelf life). Respiration rates and ethylene emissions were measured every 3 days, while flesh firmness, weight loss and total polyphenol content were evaluated at the end of the storage period and during shelf life. The respiration rate of all the genetic materials was around 27.6 mg kg-1 h-1 of CO2 during the storage period, while ethylene emission was maintained below 100 µL kg-1 h-1 without differences among maturity stages and genetic materials. Fruits harvested at the M1 stage were 40-60% more firm than fruits harvested at the M2 stage. Genetic materials identified as 95 and 98 had the highest firmness results, with values of 150.1 and 145.1 N (M1), 112.6 and 90.4 N (M2) at harvest and 28.8 and 17.4 N (M1) and 17.2 and 15.4 N (M2) after 30 days at 5°C + 7 days at 20°C, respectively. Average weight loss at the end of storage (30+7) was 14.7%. Fruits identified as mater... Presentar Todo |
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Palabras claves : |
Ethylene; Firmness; Functional quality; Respiration. |
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Thesagro : |
ACCA SELLOWIANA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03073naa a2200277 a 4500
001 1060362
005 2019-11-12
008 2018 bl uuuu u00u1 u #d
020 $a978-94-62611-90-0
022 $a0567-7572 (print) / 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2018.1194.12$2DOI
100 1 $aURRABURU, M.
245 $aPostharvest and functional behavior of some feijoa genetic materials. (Conference paper).$h[electronic resource]
260 $c2018
490 $a(Acta Horticulturae; 1194)
500 $aIn: Acta Horticulturae (ISHS) 1194: VIII International Postharvest Symposium: Enhancing Supply Chain and Consumer Benefits - Ethical and Technological Issues. Editors: F. Artés-Hernández, P.A. Gómez, E. Aguayo, F. Artés. Publication date: 4 april 2018.
520 $aABSTRACT. Feijoa (Acca sellowiana (Berg.) Burret) is one of the most popular native Uruguayan trees whose functional characteristics have attracted the interest for commercial cultivation. The aim of this work was to evaluate the postharvest behavior of six genetic materials, identified as 95, 97, 98, 152, 153 and 154 from the Facultad de Agronomía and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) breeding program. Fruits harvested at two maturity stages (M1: not easily picked; M2: touch picking) were stored for 0, 15 and 30 days at 5°C and 95% relative humidity (RH) plus 7 days at 18°C (shelf life). Respiration rates and ethylene emissions were measured every 3 days, while flesh firmness, weight loss and total polyphenol content were evaluated at the end of the storage period and during shelf life. The respiration rate of all the genetic materials was around 27.6 mg kg-1 h-1 of CO2 during the storage period, while ethylene emission was maintained below 100 µL kg-1 h-1 without differences among maturity stages and genetic materials. Fruits harvested at the M1 stage were 40-60% more firm than fruits harvested at the M2 stage. Genetic materials identified as 95 and 98 had the highest firmness results, with values of 150.1 and 145.1 N (M1), 112.6 and 90.4 N (M2) at harvest and 28.8 and 17.4 N (M1) and 17.2 and 15.4 N (M2) after 30 days at 5°C + 7 days at 20°C, respectively. Average weight loss at the end of storage (30+7) was 14.7%. Fruits identified as material 152 had the highest polyphenol content with values at harvest of 2,384.5 mg GAE 100 g-1 DW (M1) and 2,130.7 mg GAE 100 g-1 DW (M2). At the end of the storage period (30+7), total polyphenol contents decreased between 30-70% in the M1 stage and between 20-50% in the M2 stage. Materials 95, 98 and 152 are interesting for commercial production due to their firmness retention and their functional composition. © 2018 International Society for Horticultural Science. All rights reserved
650 $aACCA SELLOWIANA
653 $aEthylene
653 $aFirmness
653 $aFunctional quality
653 $aRespiration
700 1 $aAGUAYO, E.
700 1 $aZOPPOLO, R.
700 1 $aSILVEIRA, A.C.
773 $tActa Horticulturae, 2018, no. 1194, pages 73-78.
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