| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
17/02/2025 |
|
Actualizado : |
17/02/2025 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
MORAIS, A. N. P.; SOUZA, S. S.; AGUIAR, F. L. N.; GASTAL, G.D.A.; BRANDÃO, F. A. S.; SOUZA, J. A.; ÑAUPAS, L. V. S.; ALVES, K. A.; ALVES, B. G.; GASTAL, M. O.; RODRIGUES, A. P. R.; FIGUEIREDO, J. R. |
|
Afiliación : |
ANA N. P. MORAIS, Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil; SAMARA S. SOUZA, Laboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil; FRANCISCO L. N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Paraíba, Sousa, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-5317-2207; FABIANA A. S. BRANDÃO, Laboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil; JULIANY A. SOUZA, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Paraíba, Sousa, Brazil; LUCY V. S. ÑAUPAS, Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil; KELE A. ALVES, Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil; BENNER G. ALVES, Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil; MELBA O. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; ANA P. R. RODRIGUES, Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil; JOSÉ R. FIGUEIREDO, Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Ceará, Fortaleza, Brazil. |
|
Título : |
Short-term bovine ovarian tissue heterotopic autotransplantation: VEGF beneficial and detrimental effects. |
|
Complemento del título : |
Research article. |
|
Fecha de publicación : |
2025 |
|
Fuente / Imprenta : |
Molecular Reproduction and Development, February 2025, Volume 92, Issue 2, e70009. https://doi.org/10.1002/mrd.70009 |
|
ISSN : |
1098-2795 (online) |
|
DOI : |
10.1002/mrd.70009 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 21 May 2024, Revised 11 December 2024, Accepted 14 December 2024, Published online 9 February 2025. -- Corresponding author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; email:egastal@siu.edu -- Funding: The research was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES; Grant #88881.064955/2014-01,Eduardo L. Gastal and Dárcio Í. A. Teixeira). Ana N. P. Morais was the recipient of a doctorate scholarship from CAPES. -- |
|
Contenido : |
ABSTRACT.- Ovarian tissue transplantation (OTT) has been suggested as an alternative to preserving female fertility in livestock species, as currently performed in women. The OTT technique has been tested as xenografting or autografting in different body sites and animal species. Currently, there are no reports available regarding the autotransplantation of ovarian tissue using the bovine model and also testing the effect of vascular endothelial growth factor (VEGF) on graft survival. This study evaluated the effects of ovarian tissue short-term exposure to VEGF before heterotopic autotransplantation into a subcutaneous site (flank region) for 7 days in cattle. The initial finding was that after OTT and without pre-exposure to VEGF, the ovarian grafts had 42% of normal preantral follicles, which indicates a substantial step forward for this technique in cattle. Furthermore, VEGF exposure actively facilitated the process of neoangiogenesis, the proliferation capability of the stromal cells for activation of the cell cycle, maintained the balance between types I and III collagen fibers, and reduced the total collagen of the grafted tissue. Moreover, ovarian fragments previously exposed to VEGF tended to have greater follicular density; however, a detrimental effect of VEGF on follicular morphology was noticed. In conclusion, this study marks a significant step forward in bovine OTT and provides a foundation for further investigations into the specific pathways, stages, and durations of VEGF exposure to unveil strategies for refining ovarian transplantation techniques in cattle and other species. © 2025 Wiley Periodicals LLC. MenosABSTRACT.- Ovarian tissue transplantation (OTT) has been suggested as an alternative to preserving female fertility in livestock species, as currently performed in women. The OTT technique has been tested as xenografting or autografting in different body sites and animal species. Currently, there are no reports available regarding the autotransplantation of ovarian tissue using the bovine model and also testing the effect of vascular endothelial growth factor (VEGF) on graft survival. This study evaluated the effects of ovarian tissue short-term exposure to VEGF before heterotopic autotransplantation into a subcutaneous site (flank region) for 7 days in cattle. The initial finding was that after OTT and without pre-exposure to VEGF, the ovarian grafts had 42% of normal preantral follicles, which indicates a substantial step forward for this technique in cattle. Furthermore, VEGF exposure actively facilitated the process of neoangiogenesis, the proliferation capability of the stromal cells for activation of the cell cycle, maintained the balance between types I and III collagen fibers, and reduced the total collagen of the grafted tissue. Moreover, ovarian fragments previously exposed to VEGF tended to have greater follicular density; however, a detrimental effect of VEGF on follicular morphology was noticed. In conclusion, this study marks a significant step forward in bovine OTT and provides a foundation for further investigations into the specific pathways, stages, and dur... Presentar Todo |
|
Palabras claves : |
Graft; Heterotopic autotransplantation; Ovarian tissue; Ovary; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; VEGF. |
|
Asunto categoría : |
L53 Fisiología Animal - Reproducción |
|
Marc : |
LEADER 03341naa a2200361 a 4500
001 1065056
005 2025-02-17
008 2025 bl uuuu u00u1 u #d
022 $a1098-2795 (online)
024 7 $a10.1002/mrd.70009$2DOI
100 1 $aMORAIS, A. N. P.
245 $aShort-term bovine ovarian tissue heterotopic autotransplantation$bVEGF beneficial and detrimental effects.$h[electronic resource]
260 $c2025
500 $aArticle history: Received 21 May 2024, Revised 11 December 2024, Accepted 14 December 2024, Published online 9 February 2025. -- Corresponding author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; email:egastal@siu.edu -- Funding: The research was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES; Grant #88881.064955/2014-01,Eduardo L. Gastal and Dárcio Í. A. Teixeira). Ana N. P. Morais was the recipient of a doctorate scholarship from CAPES. --
520 $aABSTRACT.- Ovarian tissue transplantation (OTT) has been suggested as an alternative to preserving female fertility in livestock species, as currently performed in women. The OTT technique has been tested as xenografting or autografting in different body sites and animal species. Currently, there are no reports available regarding the autotransplantation of ovarian tissue using the bovine model and also testing the effect of vascular endothelial growth factor (VEGF) on graft survival. This study evaluated the effects of ovarian tissue short-term exposure to VEGF before heterotopic autotransplantation into a subcutaneous site (flank region) for 7 days in cattle. The initial finding was that after OTT and without pre-exposure to VEGF, the ovarian grafts had 42% of normal preantral follicles, which indicates a substantial step forward for this technique in cattle. Furthermore, VEGF exposure actively facilitated the process of neoangiogenesis, the proliferation capability of the stromal cells for activation of the cell cycle, maintained the balance between types I and III collagen fibers, and reduced the total collagen of the grafted tissue. Moreover, ovarian fragments previously exposed to VEGF tended to have greater follicular density; however, a detrimental effect of VEGF on follicular morphology was noticed. In conclusion, this study marks a significant step forward in bovine OTT and provides a foundation for further investigations into the specific pathways, stages, and durations of VEGF exposure to unveil strategies for refining ovarian transplantation techniques in cattle and other species. © 2025 Wiley Periodicals LLC.
653 $aGraft
653 $aHeterotopic autotransplantation
653 $aOvarian tissue
653 $aOvary
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aVEGF
700 1 $aSOUZA, S. S.
700 1 $aAGUIAR, F. L. N.
700 1 $aGASTAL, G.D.A.
700 1 $aBRANDÃO, F. A. S.
700 1 $aSOUZA, J. A.
700 1 $aÑAUPAS, L. V. S.
700 1 $aALVES, K. A.
700 1 $aALVES, B. G.
700 1 $aGASTAL, M. O.
700 1 $aRODRIGUES, A. P. R.
700 1 $aFIGUEIREDO, J. R.
773 $tMolecular Reproduction and Development, February 2025, Volume 92, Issue 2, e70009. https://doi.org/10.1002/mrd.70009
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 1 | |
| Registros recuperados : 1 | |
|
| Expresión de búsqueda válido. Check! |
|
|
