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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/09/2014 |
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Actualizado : |
26/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SOTELO, M.; IRISARRI, P.; LORITE, M.J.; CASARETTO, E.; REBUFFO, M.; SANJUAN, J.; MONZA, J. |
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Afiliación : |
MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diversity of rhizobia nodulating Lotus corniculatus grown in northern and southern regions of Uruguay. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Applied Soil Ecology,Volume 49, September 2011, Pages 197-207. |
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ISSN : |
0929-1393 |
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DOI : |
10.1016/j.apsoil.2011.05.005 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article history: Received 6 October 2010/Received in revised form 12 May 2011/Accepted 13 May 2011. |
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Contenido : |
Abstract:
Diversity of rhizobia nodulating Lotus corniculatus grown in geographical regions with different rainfall regimes in northern and southern Uruguay, was estimated using 168 root nodule isolates. ERIC-PCR analysis revealed no correlation between observed fingerprints and the geographical origin of isolates. Despite the commercial strain U510 has been used for decades to inoculate L. corniculatus, none of the isolates corresponded to this strain. Phylogenetic analyses using 16S rRNA and atpD genes, and ITS sequences clustered all the isolates within genus Mesorhizobium. A great majority of the isolates likely belong to the species M. huakuii, as does the commercial strain U510. The remaining isolates were closely related to either M. septentrionale or M. caraganae. Although no M. loti-like bacteria were identified, all isolates carried symbiotic genes closely related to M. loti and other narrow host range Lotus rhizobia. A significant portion of the Uruguayan isolates were as efficient as the reference strain U510 in symbiosis with L. corniculatus. A few of the isolates were also capable of nitrogen fixation in symbiosis with L. uliginosus, albeit with lower efficiency than reference strains. Our results indicate that rhizobia nodulating L. corniculatus in Uruguay are genetically and phenotypically diverse, and that the commercial strain U510 is probably not adapted to survive the Uruguayan edaphoclimatic conditions. |
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Palabras claves : |
16S RRNA; ATPD; ITS; MESORHIZOBIUM; NIFH; NODC. |
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Thesagro : |
LEGUMINOSAS FORRAJERAS; LOTUS CORNICULATUS; LOTUS ULIGINOSUS; RIZOBIOS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02504naa a2200349 a 4500
001 1050393
005 2019-11-26
008 2012 bl uuuu u00u1 u #d
022 $a0929-1393
024 7 $a10.1016/j.apsoil.2011.05.005$2DOI
100 1 $aSOTELO, M.
245 $aDiversity of rhizobia nodulating Lotus corniculatus grown in northern and southern regions of Uruguay.$h[electronic resource]
260 $c2012
500 $aArticle history: Article history: Received 6 October 2010/Received in revised form 12 May 2011/Accepted 13 May 2011.
520 $aAbstract: Diversity of rhizobia nodulating Lotus corniculatus grown in geographical regions with different rainfall regimes in northern and southern Uruguay, was estimated using 168 root nodule isolates. ERIC-PCR analysis revealed no correlation between observed fingerprints and the geographical origin of isolates. Despite the commercial strain U510 has been used for decades to inoculate L. corniculatus, none of the isolates corresponded to this strain. Phylogenetic analyses using 16S rRNA and atpD genes, and ITS sequences clustered all the isolates within genus Mesorhizobium. A great majority of the isolates likely belong to the species M. huakuii, as does the commercial strain U510. The remaining isolates were closely related to either M. septentrionale or M. caraganae. Although no M. loti-like bacteria were identified, all isolates carried symbiotic genes closely related to M. loti and other narrow host range Lotus rhizobia. A significant portion of the Uruguayan isolates were as efficient as the reference strain U510 in symbiosis with L. corniculatus. A few of the isolates were also capable of nitrogen fixation in symbiosis with L. uliginosus, albeit with lower efficiency than reference strains. Our results indicate that rhizobia nodulating L. corniculatus in Uruguay are genetically and phenotypically diverse, and that the commercial strain U510 is probably not adapted to survive the Uruguayan edaphoclimatic conditions.
650 $aLEGUMINOSAS FORRAJERAS
650 $aLOTUS CORNICULATUS
650 $aLOTUS ULIGINOSUS
650 $aRIZOBIOS
653 $a16S RRNA
653 $aATPD
653 $aITS
653 $aMESORHIZOBIUM
653 $aNIFH
653 $aNODC
700 1 $aIRISARRI, P.
700 1 $aLORITE, M.J.
700 1 $aCASARETTO, E.
700 1 $aREBUFFO, M.
700 1 $aSANJUAN, J.
700 1 $aMONZA, J.
773 $tApplied Soil Ecology,Volume 49, September 2011, Pages 197-207.
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Registro original : |
INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
23/04/2015 |
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Actualizado : |
23/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
LADO, J.; ALÓS, E.; RODRIGO, M.J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUETA ALÓS, CSIC/ IAS (Instituto de Agricultura Sostenible); MARÍA JESÚS RODRIGO, IATA (Instituto de Agroquímica y Tecnología de Alimentos); LORENZO ZACARÍAS, IATA (Instituto de Agroquímica y Tecnología de Alimentos). |
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Título : |
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Plant Science, 2015, v.231, p.138-147. |
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DOI : |
10.1016/j.plantsci.2014.12.002 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 2 October 2014 / Received in revised form 26 November 2014 / Accepted 1 December 2014 / Available online 5 December 2014. |
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Contenido : |
ABSTRACT.
Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue. |
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Palabras claves : |
VITAMINA C. |
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Thesagro : |
ANTIOXIDANTES; CITRUS; EXPRESION GENICA; VITAMINAS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02127naa a2200241 a 4500
001 1052574
005 2019-10-23
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.plantsci.2014.12.002$2DOI
100 1 $aLADO, J.
245 $aLight avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit.$h[electronic resource]
260 $c2015
500 $aArticle history: Received 2 October 2014 / Received in revised form 26 November 2014 / Accepted 1 December 2014 / Available online 5 December 2014.
520 $aABSTRACT. Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue.
650 $aANTIOXIDANTES
650 $aCITRUS
650 $aEXPRESION GENICA
650 $aVITAMINAS
653 $aVITAMINA C
700 1 $aALÓS, E.
700 1 $aRODRIGO, M.J.
700 1 $aZACARÍAS, L.
773 $tPlant Science, 2015$gv.231, p.138-147.
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