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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha actual : |
24/09/2014 |
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Actualizado : |
08/08/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BATISTA, L.; IRISARRI, L.; REBUFFO, M.; CUITIÑO, M.J.; SANJUÁN, J.; MONZA , J. |
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Afiliación : |
LETICIA BATISTA, Universidad de la República (UdelaR)/ Facultad de Agronomía; PILAR IRISARRI, Universidad de la República (UdelaR)/ Facultad de Agronomía; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA JOSE CUITIÑO DE VEGA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN SANJUÁN, Estación Experimental del Zaidín; JORGE MONZA, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
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Título : |
Nodulation competitiveness as a requisite for improved rhizobial inoculants of Trifolium pratense. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Biology and Fertility of Soils , v. 51, n. 1, p. 11-20, 2015 |
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ISSN : |
0178-2762. |
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DOI : |
10.1007/s00374-014-0946-3 |
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Idioma : |
Inglés |
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Notas : |
Received: 21 April 2014 /Revised: 14 July 2014 /Accepted: 16 July 2014 /Published online: 27 July 2014 |
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Contenido : |
ABSTRACT.
Red clover (Trifolium pratense L.) is used in the improvement of grasslands in Uruguay and has been inoculated with commercial strain U204 of Rhizobium leguminosarum bv trifolii since 1970s. Native-naturalized rhizobia strains present in soil are the basis for selecting and developing new inoculants. With this aim, we evaluated the diversity of red clover rhizobia in Uruguayan red clover pastures both historically inoculated with U204 and noninoculated ones. Thirty-eight different enterobacterial repetitive intergenic consensus (ERIC) PCR genomic fingerprints were identified, albeit surprisingly only one of 80 isolates showed an ERIC profile similar to U204. Under controlled conditions, red clover plants inoculated with one of the native isolates, strain 317, produced more biomass than those inoculated with the commercial U204. ERIC-PCR was also used to show that strain 317 competed for nodulation better than U204 in a field with previous history of inoculation. Moreover, both U204 and 317 were tagged with a gusA reporter gene and their competitiveness for nodulation assessed in various soil types. Again, strain 317 appeared more competitive than U204, particularly in soils with previous history of inoculation. Our results reinforce the long-known idea of assessing the actual needs of inoculation of legumes in different soils and suggest that the indigenous isolate 317 is an effective and competitive strain that can be used for development of a new red clover inoculant.
@ Springer-Verlag Berlin Heidelberg 2014 MenosABSTRACT.
Red clover (Trifolium pratense L.) is used in the improvement of grasslands in Uruguay and has been inoculated with commercial strain U204 of Rhizobium leguminosarum bv trifolii since 1970s. Native-naturalized rhizobia strains present in soil are the basis for selecting and developing new inoculants. With this aim, we evaluated the diversity of red clover rhizobia in Uruguayan red clover pastures both historically inoculated with U204 and noninoculated ones. Thirty-eight different enterobacterial repetitive intergenic consensus (ERIC) PCR genomic fingerprints were identified, albeit surprisingly only one of 80 isolates showed an ERIC profile similar to U204. Under controlled conditions, red clover plants inoculated with one of the native isolates, strain 317, produced more biomass than those inoculated with the commercial U204. ERIC-PCR was also used to show that strain 317 competed for nodulation better than U204 in a field with previous history of inoculation. Moreover, both U204 and 317 were tagged with a gusA reporter gene and their competitiveness for nodulation assessed in various soil types. Again, strain 317 appeared more competitive than U204, particularly in soils with previous history of inoculation. Our results reinforce the long-known idea of assessing the actual needs of inoculation of legumes in different soils and suggest that the indigenous isolate 317 is an effective and competitive strain that can be used for development of a new red clover inoc... Presentar Todo |
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Palabras claves : |
DNA FINGERPRINTING; GusA; RED CLOVER; RHIZOBIUM LEGUMINOSARUM; RHIZOBIUM LEGUMINOSARUM BV TRIFOLI; TRÉBOL ROJO; TRIFOLIUM PRATENSE. |
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Thesagro : |
MEJORAMIENTO DE LEGUMINOSAS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02569naa a2200313 a 4500
001 1054070
005 2017-08-08
008 2015 bl uuuu u00u1 u #d
022 $a0178-2762.
024 7 $a10.1007/s00374-014-0946-3$2DOI
100 1 $aBATISTA, L.
245 $aNodulation competitiveness as a requisite for improved rhizobial inoculants of Trifolium pratense.$h[electronic resource]
260 $c2015
500 $aReceived: 21 April 2014 /Revised: 14 July 2014 /Accepted: 16 July 2014 /Published online: 27 July 2014
520 $aABSTRACT. Red clover (Trifolium pratense L.) is used in the improvement of grasslands in Uruguay and has been inoculated with commercial strain U204 of Rhizobium leguminosarum bv trifolii since 1970s. Native-naturalized rhizobia strains present in soil are the basis for selecting and developing new inoculants. With this aim, we evaluated the diversity of red clover rhizobia in Uruguayan red clover pastures both historically inoculated with U204 and noninoculated ones. Thirty-eight different enterobacterial repetitive intergenic consensus (ERIC) PCR genomic fingerprints were identified, albeit surprisingly only one of 80 isolates showed an ERIC profile similar to U204. Under controlled conditions, red clover plants inoculated with one of the native isolates, strain 317, produced more biomass than those inoculated with the commercial U204. ERIC-PCR was also used to show that strain 317 competed for nodulation better than U204 in a field with previous history of inoculation. Moreover, both U204 and 317 were tagged with a gusA reporter gene and their competitiveness for nodulation assessed in various soil types. Again, strain 317 appeared more competitive than U204, particularly in soils with previous history of inoculation. Our results reinforce the long-known idea of assessing the actual needs of inoculation of legumes in different soils and suggest that the indigenous isolate 317 is an effective and competitive strain that can be used for development of a new red clover inoculant. @ Springer-Verlag Berlin Heidelberg 2014
650 $aMEJORAMIENTO DE LEGUMINOSAS
653 $aDNA FINGERPRINTING
653 $aGusA
653 $aRED CLOVER
653 $aRHIZOBIUM LEGUMINOSARUM
653 $aRHIZOBIUM LEGUMINOSARUM BV TRIFOLI
653 $aTRÉBOL ROJO
653 $aTRIFOLIUM PRATENSE
700 1 $aIRISARRI, L.
700 1 $aREBUFFO, M.
700 1 $aCUITIÑO, M.J.
700 1 $aSANJUÁN, J.
700 1 $aMONZA , J.
773 $tBiology and Fertility of Soils$gv. 51, n. 1, p. 11-20, 2015
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