01916naa a2200265 a 450000100080000000500110000800800410001902200140006002400330007410000250010724501040013226000090023650000960024552010610034165000140140265300480141665300100146465300270147465300270150165300250152870000170155370000150157070000180158577300470160310509652019-10-11       2013    bl uuuu     u00u1 u    #d  a0043-17457 a10.1614/WS-D-12-00073.12DOI1 aALARCÓN-REVERTE, R.  aResistance to Glyphosate in Junglerice (Echinochloa colona) from California.h[electronic resource]  c2013  aArticle history: Received: 14 May 2012/ Accepted: 7 August 2012/ Published: 1 January 2013.  aAbstract: A suspected glyphosate-resistant (R) junglerice population was collected from a glyphosate-R corn field near Durham in northern California where glyphosate had been applied at least twice a year for over 6 yr. Based on the amount of glyphosate required to reduce growth by 50% (ED50), the R population was 6.6 times more R than the susceptible (S) standard population. Based on the glyphosate concentration that inhibits EPSPS by 50% based on shikimate accumulation (I50) in leaf discs, R plants were four times more R than S plants. By 3 d after treatment with 0.42 kg ae ha?1 glyphosate, the S population had accumulated approximately five times more shikimate than the R population. No differences in [14C]-glyphosate uptake and translocation were detected between R and S plants. However, partial sequencing of the EPSPS gene revealed a mutation in R plants causing a proline to serine change at EPSPS position 106 (P106S). Our results reveal the first case of a P106S target site mutation associated with glyphosate resistance in junglerice.  aGLIFOSATO  a5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE  aEPSPS  aGLYPHOSATE LEAF UPTAKE  aSHIKIMATE ACCUMULATION  aTARGET SITE MUTATION1 aGARCIA, M.A.1 aURZÚA, J.1 aFISCHER, A.J.  tWeed Sciencegv. 61, n.1, p. 48-54,  2013.