03782naa a2200409 a 450000100080000000500110000800800410001902400270006010000190008724501080010626000090021450010280022352015500125165300130280165300100281465300100282465300490283465300310288365300130291465300170292770000200294470000220296470000260298670000200301270000150303270000230304770000150307070000220308570000200310770000160312770000150314370000210315870000270317970000170320670000160322377301330323910635252022-12-01       2022    bl uuuu     u00u1 u    #d7 a10.1002/mrd.236382DOI1 aPASSOS, J.R.S.  aHow in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.h[electronic resource]  c2022  aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil.  aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC.  aFOLLICLE  aOVARY  aOVINE  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL  aPLATAFORMA DE SALUD ANIMAL  aPROTEINS  aREPRODUCTION1 aGUERREIRO, D.D.1 aKAMILA S. OTÁVIO1 aDOS SANTOS-NETO, P.C.1 aSOUZA-NEVES, M.1 aCUADRO, F.1 aNUÑEZ?OLIVERA, R.1 aCRISPO, M.1 aVASCONCELOS, F.R.1 aBEZERRA, M.J.B.1 aSILVA, R.F.1 aLIMA, L.F.1 aFIGUEIREDO, J.R.1 aBUSTAMANTE-FILHO, I.C.1 aMENCHACA, A.1 aMOURA, A.A.  tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638