02882naa a2200265 a 450000100080000000500110000800800410001902200230006002400390008310000180012224501180014026000090025850006610026752013100092865300150223865300130225365300260226665300080229265300220230070000170232270000160233970000180235570000160237377302270238910637782022-11-25       2022    bl uuuu     u00u1 u    #d  a1366-5863 (online)7 a10.1080/09670874.2022.21188942DOI1 aLARZÁBAL, J.  aIntrogression of Asian soybean rust resistant genes into elite soybean lines from Uruguay.h[electronic resource]  c2022  aArticle history: Received 03 May 2022, Accepted 23 August 2022, Published online: 11 November 2022. Corresponding author: Silvina Stewart, Instituto Nacional de Investigación Agropecuaria (INIA), Programa Nacional de Cultivos de Secano, Colonia, Uruguay.  emailto: sstewart@inia.org.uy -- Funding:  This study was partly financially supported by the National Institute for Agricultural Research (INIA) and partly by the Japan International Research Center for Agricultural Sciences (JIRCAS) research project "Development of resilient crops and production technologies." -- Supplementary material can be accessed at http://doi:10.1080/09670874.2022.2118894  aABSTRACT.- Soybean is the main agricultural crop in Uruguay. One of the diseases with the greatest damage potential is Asian soybean rust, with estimated grain yield losses up 80% in the region. Throughout history, different authors have stated the importance of genetic resistance as a fundamental tool to decrease the adverse effects of phytopathogens, leading to agriculture sustainability. The objective of this work was to introduce rust resistance genes into elite lines of INIA's soybean breeding program and to evaluate the enhanced resistance. Introgression of resistant genes was carried out in a greenhouse, through three backcrosses from F1. Two donors: Py7-1-47 and No6-12-B handed by JIRCAS with two resistant genes combinations (Rpp1-b + Rpp5 and Rpp4 + Rpp5, respectively) and three elite lines: SJ10-122-040, SJ10-158-039, and SJ10-173-072 from INIA's breeding program were used. After each backcross, plants carrying both genes were selected with marker assisted selection. Each third backcross was self-pollinated (BC3F2) and single plant families were inoculated in the greenhouse. Percentage of rust resistant plants within the introgressed families ranged from 42% to 98%, compared to fully susceptible recurrent parents. © 2022 Informa UK Limited, trading as Taylor & Francis Group  aBiotrophic  aBreeding  aPhakopsora pachyrhizi  aRpp  aSegregation ratio1 aYAMANAKA, N.1 aCERETTA, S.1 aRODRIGUEZ, M.1 aSTEWART, S.  tInternational Journal of Pest Management, 2022, vol. 68, issue 4: "Uruguayan Society of Phytopathology (SUFIT): Plant protection for a sustainable agriculture", p.319-327. doi: https://doi.org/10.1080/09670874.2022.2118894