02746naa a2200361 a 450000100080000000500110000800800410001902200140006002400350007410000250010924501260013426000090026050003410026952013640061065300260197465300200200065300560202065300180207665300140209465300100210865300100211870000190212870000140214770000170216170000170217870000160219570000170221170000160222870000140224470000180225870000160227677300920229210650532025-02-14       2025    bl uuuu     u00u1 u    #d  a0300-98587 a10.1177/030098582413093942DOI1 aGARCÍA Y SANTOS, C.  aAstylus atromaculatus (pollen beetle) gastrointestinal disease experimentally reproduced in sheep.h[electronic resource]  c2025  aArticle history: Article first published online 30 January 2025. -- Corresponding author: Uzal, F.A.; California Animal HealthFood Safety Laboratory, School of Veterinary Medicine, University of California, Davis, 105 West Central Avenue, San Bernardino, CA, United States; email:fauzal@ucdavis.edu  -- Publisher: SAGE Publications Inc.  aABSTRACT.- Intoxication of sheep and cattle by Astylus atromaculatus recently occurred in Uruguay and Argentina in association with severe drought. Although the disease was experimentally reproduced in sheep in the 1970s, there is limited information on clinical and pathologic findings of sheep experimentally intoxicated by this beetle. Here, we described the clinical, gross, and microscopic findings in 3 sheep orally dosed with A. atromaculatus (treatment group, TG) and in 2 control sheep (control group, CG) dosed with distilled water. Anorexia, lethargy, ruminal stasis, reluctance to move, prolonged recumbency, and bruxism were observed in the TG but not the CG sheep. Gross postmortem lesions were only observed in TG sheep and included fibrinonecrotizing enteritis affecting the duodenum, jejunum, and ileum, and multifocal hemorrhages in rumen, omasum, and abomasum. Microscopically, all 3 TG animals had multifocal necrosis in the small intestine; the lesions were most severe in the jejunum. Multifocal necrosis was seen in the mucosa of the rumen, omasum, and abomasum. No significant gross or microscopic abnormalities were observed in the 2 CG sheep. The study supports the role of A. atromaculatus in acute, fatal gastrointestinal disease like that previously described in experimental and spontaneous cases in sheep. © The Author(s) 2025.  aAstylus atromaculatus  aGastroenteritis  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA  aPollen beetle  aRuminants  aSheep  aToxic1 aGARCÍA, J. A.1 aDUTRA, F.1 aLIVIO, J. M.1 aCORRO, A. C.1 aCANTÓN, G.1 aESCALONA, J.1 aCAPELLI, A.1 aMATTO, C.1 aGIANNITTI, F.1 aUZAL, F. A.  tVeterinary Pathology, 2025. https://doi.org/10.1177/03009858241309394  -- [Online firt]