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| 1. |  | QUEZADA, M.; PASTINA, M.M.; RAVEST, G.; SILVA, P.; VIGNALE, B.; CABRERA, D.; HINRICHSEN, P.; GARCÍA, A.A.F.; PRITSCH, C. A first genetic map of Acca sellowiana based on ISSR, AFLP and SSR markers. Scientia Horticulturae, 2014, v.169, no.2, p. 138-146. Article history: Received 13 May 2013 / Received in revised form 24 January 2014 / Accepted 12 February 2014 / Available online 14 March 2014.| Biblioteca(s): INIA Las Brujas. |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/06/2023 |
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Actualizado : |
28/06/2023 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
DE TRAVENSOLO, R.F.; FERREIRA, V.G.; FEDERICI, M.; DE LEMOS, E.G.M.; CARRILHO, E. |
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Afiliación : |
REGIANE FÁTIMA DE TRAVENSOLO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; VINÍCIUS GUIMARÃES FERREIRA, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELIANA GERTRUDES MACEDO DE LEMOS, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, Jaboticabal, SP, Brazil; EMANUEL CARRILHO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil. |
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Título : |
Microarrays application in life sciences: The beginning of the revolution. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
In: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25 |
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ISBN : |
978-303082380-1; eISBN: 978-303082381-8 |
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DOI : |
10.1007/978-3-030-82381-8_25 |
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Idioma : |
Inglés |
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Notas : |
Correspondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br -- |
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Contenido : |
Previous to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd. |
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Palabras claves : |
DNA analysis; Microarray applications; Microarrays. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 01965naa a2200229 a 4500
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024 7 $a10.1007/978-3-030-82381-8_25$2DOI
100 1 $aDE TRAVENSOLO, R.F.
245 $aMicroarrays application in life sciences$bThe beginning of the revolution.$h[electronic resource]
260 $c2022
500 $aCorrespondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br --
520 $aPrevious to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd.
653 $aDNA analysis
653 $aMicroarray applications
653 $aMicroarrays
700 1 $aFERREIRA, V.G.
700 1 $aFEDERICI, M.
700 1 $aDE LEMOS, E.G.M.
700 1 $aCARRILHO, E.
773 $tIn: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25
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