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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
01/10/2019 |
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Actualizado : |
01/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; CARRO, B. L.; DALLA RIZZA, M.; REYNO, R. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; B. LÓPEZ CARRO, Flow Cytometry and Cell Sorting Core, Institute of Biological Research Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Use of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum. |
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Complemento del título : |
Conference Paper. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Acta Horticulturae, 15 November 2018, N° 1224, p. 73-80. |
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Serie : |
(Acta Horticulturae; 1224) |
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ISBN : |
978-94-62612-20-4 |
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ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2018.1224.11 |
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Idioma : |
Inglés |
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Notas : |
In: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva. |
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Contenido : |
ABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal
guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry.
@ISHS, International Society for Horticultural Science. MenosABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine r... Presentar Todo |
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Palabras claves : |
BIOMASS; COLCHICINE; ORYZALIN; POLYPLOIDY. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02755naa a2200265 a 4500
001 1060254
005 2019-10-01
008 2018 bl uuuu u00u1 u #d
020 $a978-94-62612-20-4
022 $a0567-7572 (print) / 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2018.1224.11$2DOI
100 1 $aCASTILLO, A.
245 $aUse of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum.$h[electronic resource]
260 $c2018
490 $a(Acta Horticulturae; 1224)
500 $aIn: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva.
520 $aABSTRACT. The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry. @ISHS, International Society for Horticultural Science.
653 $aBIOMASS
653 $aCOLCHICINE
653 $aORYZALIN
653 $aPOLYPLOIDY
700 1 $aCARRO, B. L.
700 1 $aDALLA RIZZA, M.
700 1 $aREYNO, R.
773 $tActa Horticulturae, 15 November 2018, N° 1224, p. 73-80.
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| 1. |  | VISSANI, M. A.; ALAMOS, F.; TORDOYA, M. S.; MINATEL, L.; SCHAMMAS, J. M.; DUS SANTOS, M. J.; TRONO, K.; BARRANDEGUY, M. E.; BALASURIYA, U. B. R.; CAROSSINO, M. Outbreak of western equine encephalitis virus infection associated with neurological disease in horses following a nearly 40-year intermission period in Argentina. Viruses, 2024, Volume 16, Issue 10, 1594. https://doi.org/10.3390/v16101594 -- OPEN ACCESS. Article history: Submission received 4 September 2024, Revised 6 October 2024, Accepted 7 October 2024, Published 10 October 2024. --
Correspondence: Vissani, M.A.; Instituto de Virología, CICVyA, Instituto Nacional de Tecnología...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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