| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
06/05/2021 |
|
Actualizado : |
10/08/2021 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
NARANCIO, R.; JOHN, U.; MASON, J.; GIRALDO, P.; SPANGENBERG, G. |
|
Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; School of Applied Systems Biology, La Trobe University, Melbourne, VIC, Australia; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Melbourne, VIC, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Melbourne, VIC, Australia; JOHN MASON, School of Applied Systems Biology, La Trobe University, Melbourne, VIC, Australia; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Melbourne, VIC, Australia; PAULA GIRALDO, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Melbourne, VIC, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia; GERMAN SPANGENBERG, School of Applied Systems Biology, La Trobe University, Melbourne, VIC, Australia; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Melbourne, VIC, Australia. |
|
Título : |
Digital PCR (dPCR) and qPCR mediated determination of transgene copy number in the forage legume white clover (Trifolium repens). |
|
Fecha de publicación : |
2021 |
|
Fuente / Imprenta : |
Molecular Biology Reports, 2021, volume 48, Issue 4, Pages 3069 - 3077. Doi: https://doi.org/10.1007/s11033-021-06354-5 |
|
ISSN : |
0301-4851 |
|
DOI : |
10.1007/s11033-021-06354-5 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received, 03 February 2021; Accepted, 12 April 2021; Published, 16 April 2021.
This project was funded by Agriculture Victoria, and Dairy Australia. |
|
Contenido : |
ABSTRACT.
Obtaining data on transgene copy number is an integral step in the generation of transgenic plants. Techniques such as Southern blot, segregation analysis, and quantitative PCR (qPCR) have routinely been used for this task, in a range of species. More recently, use of Digital PCR (dPCR) has become prevalent, with a measurement accuracy higher than qPCR reported. Here, the relative merits of qPCR and dPCR for transgene copy number estimation in white clover were investigated. Furthermore, given that single copy reference genes are desirable for estimating gene copy number by relative quantification, and that no single-copy genes have been reported in this species, a search and evaluation of suitable reference genes in white clover was undertaken. Results demonstrated a higher accuracy of dPCR relative to qPCR for copy number estimation in white clover. Two genes, Pyruvate dehydrogenase (PDH), and an ATP-dependent protease, identified as single-copy genes, were used as references for copy number estimation by relative quantification. Identification of single-copy genes in white clover will enable the application of relative quantification for copy number estimation of other genes or transgenes in the species. The results generated here validate the use of dPCR as a reliable strategy for transgene copy number estimation in white clover, and provide resources for future copy number studies in this species.
© 2021, The Author(s), under exclusive licence to Springer Nature B.V. MenosABSTRACT.
Obtaining data on transgene copy number is an integral step in the generation of transgenic plants. Techniques such as Southern blot, segregation analysis, and quantitative PCR (qPCR) have routinely been used for this task, in a range of species. More recently, use of Digital PCR (dPCR) has become prevalent, with a measurement accuracy higher than qPCR reported. Here, the relative merits of qPCR and dPCR for transgene copy number estimation in white clover were investigated. Furthermore, given that single copy reference genes are desirable for estimating gene copy number by relative quantification, and that no single-copy genes have been reported in this species, a search and evaluation of suitable reference genes in white clover was undertaken. Results demonstrated a higher accuracy of dPCR relative to qPCR for copy number estimation in white clover. Two genes, Pyruvate dehydrogenase (PDH), and an ATP-dependent protease, identified as single-copy genes, were used as references for copy number estimation by relative quantification. Identification of single-copy genes in white clover will enable the application of relative quantification for copy number estimation of other genes or transgenes in the species. The results generated here validate the use of dPCR as a reliable strategy for transgene copy number estimation in white clover, and provide resources for future copy number studies in this species.
© 2021, The Author(s), under exclusive licence to Springer Nat... Presentar Todo |
|
Palabras claves : |
DdPCR; Reference genes; Relative quantification; Single copy genes; Trifolium repens; Zygosity determination. |
|
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
|
Marc : |
LEADER 02569naa a2200277 a 4500
001 1062022
005 2021-08-10
008 2021 bl uuuu u00u1 u #d
022 $a0301-4851
024 7 $a10.1007/s11033-021-06354-5$2DOI
100 1 $aNARANCIO, R.
245 $aDigital PCR (dPCR) and qPCR mediated determination of transgene copy number in the forage legume white clover (Trifolium repens).$h[electronic resource]
260 $c2021
500 $aArticle history: Received, 03 February 2021; Accepted, 12 April 2021; Published, 16 April 2021. This project was funded by Agriculture Victoria, and Dairy Australia.
520 $aABSTRACT. Obtaining data on transgene copy number is an integral step in the generation of transgenic plants. Techniques such as Southern blot, segregation analysis, and quantitative PCR (qPCR) have routinely been used for this task, in a range of species. More recently, use of Digital PCR (dPCR) has become prevalent, with a measurement accuracy higher than qPCR reported. Here, the relative merits of qPCR and dPCR for transgene copy number estimation in white clover were investigated. Furthermore, given that single copy reference genes are desirable for estimating gene copy number by relative quantification, and that no single-copy genes have been reported in this species, a search and evaluation of suitable reference genes in white clover was undertaken. Results demonstrated a higher accuracy of dPCR relative to qPCR for copy number estimation in white clover. Two genes, Pyruvate dehydrogenase (PDH), and an ATP-dependent protease, identified as single-copy genes, were used as references for copy number estimation by relative quantification. Identification of single-copy genes in white clover will enable the application of relative quantification for copy number estimation of other genes or transgenes in the species. The results generated here validate the use of dPCR as a reliable strategy for transgene copy number estimation in white clover, and provide resources for future copy number studies in this species. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
653 $aDdPCR
653 $aReference genes
653 $aRelative quantification
653 $aSingle copy genes
653 $aTrifolium repens
653 $aZygosity determination
700 1 $aJOHN, U.
700 1 $aMASON, J.
700 1 $aGIRALDO, P.
700 1 $aSPANGENBERG, G.
773 $tMolecular Biology Reports, 2021, volume 48, Issue 4, Pages 3069 - 3077. Doi: https://doi.org/10.1007/s11033-021-06354-5
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha actual : |
02/06/2022 |
|
Actualizado : |
02/06/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
KROLOW, T. K.; LUCAS, M.; HENRIQUES, A. L. |
|
Afiliación : |
TIAGO KÜTTER KROLOW, Universidade Federal do Tocantins (UFT), Programa de Pós-Graduação em Biodiversidade, Ecologia e Conservação (PPGBec), TO, Porto Nacional, Brazil; MARTÍN LUCAS FONSECA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; AUGUSTO LOUREIRO HENRIQUES, Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Biodiversidade, AM, Manaus, Brazil. |
|
Título : |
Revisiting the Tabanid fauna (Diptera: Tabanidae) of Uruguay: notes on the species of the genus Tabanus Linnaeus, with the description of a new species. |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Neotropical Entomology, 2022, vol. 51, pages 447- 457. doi: https://doi.org/10.1007/s13744-022-00958-7 |
|
ISSN : |
1519-566X |
|
DOI : |
10.1007/s13744-022-00958-7 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 5 July 2021; Accepted 1 April 2022; Published online 11 May 2022.
Corresponding author: Krolow, T.K.; Universidade Federal do Tocantins (UFT), Programa de Pós-Graduação em Biodiversidade, Ecologia e Conservação (PPGBec), TO, Porto Nacional, Brazil; email:tkkrolow@gmail.com -- INIA Tacuarembó as part of the project ?Control of horn fly through the selective treatment of bulls and the importance of Horse flies (Diptera: Tabanidae) as vectors of Anaplasma marginale in the northern region of the country.? Tiago Kütter Krolow received a research grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-310214/2021?1). |
|
Contenido : |
ABSTRACT - We describe a new species of Tabanus (for Uruguay, the first in more than 90 years) based on females from the Department of Tacuarembó, Uruguay. Tabanus tacuaremboensissp. n. is the 15th species of the genus Tabanus recorded from Uruguay. The species description is accompanied by illustrations of the diagnostic external and internal characters. In addition, for comparison with the new species, we provide photographs of the regional species of Tabanus whose distributions are still poorly known. In view of the lengthy hiatus since the last new tabanid species was described from Uruguay, we offer a brief discussion of the importance of new and further sampling in areas where the tabanofauna is still poorly known. © 2022, Sociedade Entomológica do Brasil. |
|
Palabras claves : |
Horse flies; Pampa; PLATAFORMA SALUD ANIMAL; Tabanini; Tacuarembó; Taxonomy. |
|
Asunto categoría : |
L40 Estructura animal |
|
Marc : |
LEADER 02271naa a2200253 a 4500
001 1063201
005 2022-06-02
008 2022 bl uuuu u00u1 u #d
022 $a1519-566X
024 7 $a10.1007/s13744-022-00958-7$2DOI
100 1 $aKROLOW, T. K.
245 $aRevisiting the Tabanid fauna (Diptera$bTabanidae) of Uruguay: notes on the species of the genus Tabanus Linnaeus, with the description of a new species.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 5 July 2021; Accepted 1 April 2022; Published online 11 May 2022. Corresponding author: Krolow, T.K.; Universidade Federal do Tocantins (UFT), Programa de Pós-Graduação em Biodiversidade, Ecologia e Conservação (PPGBec), TO, Porto Nacional, Brazil; email:tkkrolow@gmail.com -- INIA Tacuarembó as part of the project ?Control of horn fly through the selective treatment of bulls and the importance of Horse flies (Diptera: Tabanidae) as vectors of Anaplasma marginale in the northern region of the country.? Tiago Kütter Krolow received a research grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-310214/2021?1).
520 $aABSTRACT - We describe a new species of Tabanus (for Uruguay, the first in more than 90 years) based on females from the Department of Tacuarembó, Uruguay. Tabanus tacuaremboensissp. n. is the 15th species of the genus Tabanus recorded from Uruguay. The species description is accompanied by illustrations of the diagnostic external and internal characters. In addition, for comparison with the new species, we provide photographs of the regional species of Tabanus whose distributions are still poorly known. In view of the lengthy hiatus since the last new tabanid species was described from Uruguay, we offer a brief discussion of the importance of new and further sampling in areas where the tabanofauna is still poorly known. © 2022, Sociedade Entomológica do Brasil.
653 $aHorse flies
653 $aPampa
653 $aPLATAFORMA SALUD ANIMAL
653 $aTabanini
653 $aTacuarembó
653 $aTaxonomy
700 1 $aLUCAS, M.
700 1 $aHENRIQUES, A. L.
773 $tNeotropical Entomology, 2022, vol. 51, pages 447- 457. doi: https://doi.org/10.1007/s13744-022-00958-7
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
