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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/12/2024 |
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Actualizado : |
26/12/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GONZÁLEZ-GHIENA, A. C.; RUBIO, L.; MENA, E.; DELGADO-CERRONE, L.; GONZÁLEZ-ARCOS, M.; PONCE DE LEÓN, I. |
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Afiliación : |
ANA CLARA GONZÁLEZ-GHIENA, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-3489-4001; EILYN MENA, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; LEONARDO DELGADO-CERRONE, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; INÉS PONCE DE LEÓN, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Genetic diversity, morphological characteristics and virulence of Stemphylium species causing tomato leaf spot disease in Uruguay. |
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Fecha de publicación : |
2024 |
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Fuente / Imprenta : |
Plant Pathology, 2024. https://doi.org/10.1111/ppa.14045 |
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ISSN : |
0032-0862 |
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DOI : |
10.1111/ppa.14045 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 02 September 2024, Revised 30 October 2024, Accepted 22 November 2024, Record online: 06 December 2024. -- Corresponding: Ponce de León, I.; Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo, Uruguay; email:iponce@iibce.edu.uy -- Funding: Agencia Nacional de Investigación e Innovación. Grant Number: FCE_1_2021_1_166555; Programa de Desarrollo de las Ciencias Básicas. -- Include Supporting information. |
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Contenido : |
ABSTRACT.- Tomato leaf spot, caused by the ascomycete fungi Stemphylium spp., including S. lycopersici, S. solani and S. vesicarium, is a severe threat to tomato production worldwide. In Uruguay, the disease has become more prevalent in both field and greenhouse production systems in recent years, primarily due to the use of susceptible tomato varieties. This study focuses on the identification, morphological characteristics, genetic diversity and virulence of Stemphylium isolates responsible for leaf spot in Uruguay's major tomato-producing regions. Phylogenetic analysis using partial sequences of the rDNA internal transcribed spacer (ITS) and glyceraldehyde-3-phosphate dehydrogenase (gpd) genes showed that S. lycopersici is the predominant pathogen, with 33 isolates identified, whereas only three isolates were classified as S. vesicarium. Genetic diversity analysis using inter simple sequence repeat (ISSR) markers identified two main clusters of S. lycopersici isolates that correlate with distinct geographical regions. Cultural and morphological characteristics of S. lycopersici varied significantly and pathogenicity assays revealed a broad spectrum of virulence among S. lycopersici isolates, with some isolates causing extensive necrosis and others showing minimal disease lesions. S. lycopersici primarily penetrates plant tissues through stomata and trichomes, and hyphae proliferate both within and on plant tissues, causing chlorosis and necrosis. Infected tissues exhibit alterations in cell wall composition associated with the incorporation of phenolic compounds. This is the first report characterizing various isolates of S. lycopersici and S. vesicarium responsible for tomato leaf spot in Uruguay. © 2024 British Society for Plant Pathology. MenosABSTRACT.- Tomato leaf spot, caused by the ascomycete fungi Stemphylium spp., including S. lycopersici, S. solani and S. vesicarium, is a severe threat to tomato production worldwide. In Uruguay, the disease has become more prevalent in both field and greenhouse production systems in recent years, primarily due to the use of susceptible tomato varieties. This study focuses on the identification, morphological characteristics, genetic diversity and virulence of Stemphylium isolates responsible for leaf spot in Uruguay's major tomato-producing regions. Phylogenetic analysis using partial sequences of the rDNA internal transcribed spacer (ITS) and glyceraldehyde-3-phosphate dehydrogenase (gpd) genes showed that S. lycopersici is the predominant pathogen, with 33 isolates identified, whereas only three isolates were classified as S. vesicarium. Genetic diversity analysis using inter simple sequence repeat (ISSR) markers identified two main clusters of S. lycopersici isolates that correlate with distinct geographical regions. Cultural and morphological characteristics of S. lycopersici varied significantly and pathogenicity assays revealed a broad spectrum of virulence among S. lycopersici isolates, with some isolates causing extensive necrosis and others showing minimal disease lesions. S. lycopersici primarily penetrates plant tissues through stomata and trichomes, and hyphae proliferate both within and on plant tissues, causing chlorosis and necrosis. Infected tissues exhibit ... Presentar Todo |
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Palabras claves : |
Disease symptoms; Genetic diversity; Leaf spot disease; SISTEMA VEGETAL INTENSIVO - INIA; Stemphylium; Tomato; Virulence. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03197naa a2200301 a 4500
001 1065019
005 2024-12-26
008 2024 bl uuuu u00u1 u #d
022 $a0032-0862
024 7 $a10.1111/ppa.14045$2DOI
100 1 $aGONZÁLEZ-GHIENA, A. C.
245 $aGenetic diversity, morphological characteristics and virulence of Stemphylium species causing tomato leaf spot disease in Uruguay.$h[electronic resource]
260 $c2024
500 $aArticle history: Received 02 September 2024, Revised 30 October 2024, Accepted 22 November 2024, Record online: 06 December 2024. -- Corresponding: Ponce de León, I.; Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo, Uruguay; email:iponce@iibce.edu.uy -- Funding: Agencia Nacional de Investigación e Innovación. Grant Number: FCE_1_2021_1_166555; Programa de Desarrollo de las Ciencias Básicas. -- Include Supporting information.
520 $aABSTRACT.- Tomato leaf spot, caused by the ascomycete fungi Stemphylium spp., including S. lycopersici, S. solani and S. vesicarium, is a severe threat to tomato production worldwide. In Uruguay, the disease has become more prevalent in both field and greenhouse production systems in recent years, primarily due to the use of susceptible tomato varieties. This study focuses on the identification, morphological characteristics, genetic diversity and virulence of Stemphylium isolates responsible for leaf spot in Uruguay's major tomato-producing regions. Phylogenetic analysis using partial sequences of the rDNA internal transcribed spacer (ITS) and glyceraldehyde-3-phosphate dehydrogenase (gpd) genes showed that S. lycopersici is the predominant pathogen, with 33 isolates identified, whereas only three isolates were classified as S. vesicarium. Genetic diversity analysis using inter simple sequence repeat (ISSR) markers identified two main clusters of S. lycopersici isolates that correlate with distinct geographical regions. Cultural and morphological characteristics of S. lycopersici varied significantly and pathogenicity assays revealed a broad spectrum of virulence among S. lycopersici isolates, with some isolates causing extensive necrosis and others showing minimal disease lesions. S. lycopersici primarily penetrates plant tissues through stomata and trichomes, and hyphae proliferate both within and on plant tissues, causing chlorosis and necrosis. Infected tissues exhibit alterations in cell wall composition associated with the incorporation of phenolic compounds. This is the first report characterizing various isolates of S. lycopersici and S. vesicarium responsible for tomato leaf spot in Uruguay. © 2024 British Society for Plant Pathology.
653 $aDisease symptoms
653 $aGenetic diversity
653 $aLeaf spot disease
653 $aSISTEMA VEGETAL INTENSIVO - INIA
653 $aStemphylium
653 $aTomato
653 $aVirulence
700 1 $aRUBIO, L.
700 1 $aMENA, E.
700 1 $aDELGADO-CERRONE, L.
700 1 $aGONZÁLEZ-ARCOS, M.
700 1 $aPONCE DE LEÓN, I.
773 $tPlant Pathology, 2024. https://doi.org/10.1111/ppa.14045
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| 1. |  | GAIERO, P.; ANDINO, M.; VAIO, M.; VIDAL, R.; ABAD-NJERS, G.; AMARILLO, A.; SILVA, S.; HERNÁNDEZ, N.; RAMOS, S.; STANCOV, V.; MOREIRA, L.; HEIDEN, G.; NICOLAO, R.; TORANZA, C.; CASTILLO, A.; IBÁÑEZ, F.; RODRÍGUEZ, G.; GONZÁLEZ-ARCOS, M.; GALVÁN, G.; SIRI, M.I.; VILARÓ, F.; SPERANZA, P. Identificación de grupos genéticos y distribución de la variabilidad de papas silvestres para su conservación en colecciones núcleo y uso en mejoramiento genético. [Resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Hortícola. Resúmenes. Jornada Mejoramiento Genético de Hortalizas: Ciencia y Tecnología para la producción y el consumidor, 2019, Salto, Uruguay. Trabajos de investigación relacionados al proyecto. Salto (UY): INIA, 2019. p. 40-41.| Tipo: Abstracts/Resúmenes |
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