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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
17/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. |
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Afiliación : |
VIRGINIA FERREIRA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARÍA J. PIANZZOLA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la República, Canelones, Uruguay; MARÍA L. TONDO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARÍA V. RODRÍGUEZ, Área Biología Vegetal (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; ELENA G. ORELLANO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARC VALSS, Center for Research in Agricultural Genomics, CSIC, IRTA, Barcelona, Spain; Department of Genetics, Universitat de Barcelona, Barcelona, Spain; MARÍA I. SIRI, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. |
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ISSN : |
1664-462X |
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DOI : |
10.3389/fpls.2017.01424 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. |
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Contenido : |
ABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.
© 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. MenosABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typic... Presentar Todo |
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Palabras claves : |
Bacterial wilt; Disease resistance; Latent infections; Plant breeding; Potato; Ralstonia solanacearum; Solanum commersonii. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03620naa a2200337 a 4500
001 1012685
005 2019-10-17
008 2017 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2017.01424$2DOI
100 1 $aFERREIRA, V.
245 $aInterspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017.
520 $aABSTRACT. Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato. © 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri.
653 $aBacterial wilt
653 $aDisease resistance
653 $aLatent infections
653 $aPlant breeding
653 $aPotato
653 $aRalstonia solanacearum
653 $aSolanum commersonii
700 1 $aPIANZZOLA, M.J.
700 1 $aVILARÓ, F.
700 1 $aGALVÁN, G.
700 1 $aTONDO, M.L.
700 1 $aRODRÍGUEZ, M.V.
700 1 $aORELLANO, E.G.
700 1 $aVALSS, M.
700 1 $aSIRI, M.I.
773 $tFrontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
23/07/2020 |
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Actualizado : |
23/07/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
TALMÓN, D.; GARCIA-ROCHE, M; MENDOZA, A.; MATTIAUDA, D.A.; CARRIQUIRY, M |
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Afiliación : |
DANIEL TALMÓN, Daniel Talmón Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900 Montevideo, Uruguay; MERCEDES GARCIA-ROCHE, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900 Montevideo, Uruguay.; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO A MATTIAUDA, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900 Montevideo, Uruguay; MARIANA CARRIQUIRY, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900 Montevideo, Uruguay. |
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Título : |
Energy partitioning and energy efficiency of two Holstein genotypes under a mixed pasture-based system during mid and late lactation. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Livestock Science, September 2020, Volume 239, Article 104166. Doi: https://doi.org/10.1016/j.livsci.2020.104166 |
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DOI : |
10.1016/j.livsci.2020.104166 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 February 2020; Received in revised form 9 July 2020; Accepted 9 July 2020. Corresponding author:
E-mail address: danieltalmon94@gmail.com (D. Talmón). |
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Contenido : |
Abstract:
The objective was to evaluate the effect of the Holstein genotype (North American Holstein vs. New Zealand Holstein; NAH vs. NZH, respectively) in a pasture-based system on heat production (HP), energy partitioning between maintenance and production (milk and tissue) and energy efficiency during two different stages of lactation. Twenty-eight Holstein dairy cows (14 cows of each genotype) with similar calving date (May 5, 2018 ± 23 days) were managed in a pasture-based system and supplemented with one third of the predicted total dry matter intake as concentrate. Heat production, retained energy in milk and tissue, metabolizable energy intake (MEI) and the proportion of MEI retained in milk + tissue (RE/MEI) were measured at 115 and 192 ± 19 days in milk and residual HP was estimated by the difference between measured HP and predicted HP based on NRC (2001) model according to body weight, body condition score and milk production. The NAH cows were 60 ± 15 kg heavier and produced 4.7 ± 1.0 kg/d more milk with lower percentages of fat and protein than NZH cows. However, there were no differences in fat or protein yield per day between genotypes. Metabolizable energy intake, retained energy in milk and tissue, HP and RE/MEI were not different between genotypes at 115 days in milk. Nevertheless, at 192 days in milk the MEI, HP and residual HP were lower in NZH than NAH, whereas RE/MEI was not different when both genotypes were managed under a pasture-based system with one third of the consumed diet as concentrate. The capacity of NZH cows to maintain the same RE/MEI than NAH cows at 192 days in milk despite of the lower MEI, was due to a lower metabolizable energy requirement for maintenance (853 vs. 729 kJ/body weight0.75 per day for NAH and NZH, respectively). Indeed, the lower energy requirement for maintenance in NZH was associated with a lower fasting heat production since kl were not different between genotypes. Thus, NZH cows could have a lower proportion of their body as protein mass or a lower relative mass of the internal organs involved with digestive and circulatory functions. However, further investigation is necessary to understand the differences in maintenance energy requirements between the Holstein genotypes. MenosAbstract:
The objective was to evaluate the effect of the Holstein genotype (North American Holstein vs. New Zealand Holstein; NAH vs. NZH, respectively) in a pasture-based system on heat production (HP), energy partitioning between maintenance and production (milk and tissue) and energy efficiency during two different stages of lactation. Twenty-eight Holstein dairy cows (14 cows of each genotype) with similar calving date (May 5, 2018 ± 23 days) were managed in a pasture-based system and supplemented with one third of the predicted total dry matter intake as concentrate. Heat production, retained energy in milk and tissue, metabolizable energy intake (MEI) and the proportion of MEI retained in milk + tissue (RE/MEI) were measured at 115 and 192 ± 19 days in milk and residual HP was estimated by the difference between measured HP and predicted HP based on NRC (2001) model according to body weight, body condition score and milk production. The NAH cows were 60 ± 15 kg heavier and produced 4.7 ± 1.0 kg/d more milk with lower percentages of fat and protein than NZH cows. However, there were no differences in fat or protein yield per day between genotypes. Metabolizable energy intake, retained energy in milk and tissue, HP and RE/MEI were not different between genotypes at 115 days in milk. Nevertheless, at 192 days in milk the MEI, HP and residual HP were lower in NZH than NAH, whereas RE/MEI was not different when both genotypes were managed under a pasture-based system with ... Presentar Todo |
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Palabras claves : |
CALORIMETRÍA INDIRECTA; DAIRY CATTLE; ENERGY EXPENDTURE; GASTO DE ENERGÍA; GRAZING SYSTEM; HOLSTEIN-FRIESIAN; INDIRECT CALORIMETRY; SISTEMA DE PASTOREO. |
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Thesagro : |
VACAS LECHERAS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03441naa a2200301 a 4500
001 1061235
005 2020-07-23
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.livsci.2020.104166$2DOI
100 1 $aTALMÓN, D.
245 $aEnergy partitioning and energy efficiency of two Holstein genotypes under a mixed pasture-based system during mid and late lactation.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 28 February 2020; Received in revised form 9 July 2020; Accepted 9 July 2020. Corresponding author: E-mail address: danieltalmon94@gmail.com (D. Talmón).
520 $aAbstract: The objective was to evaluate the effect of the Holstein genotype (North American Holstein vs. New Zealand Holstein; NAH vs. NZH, respectively) in a pasture-based system on heat production (HP), energy partitioning between maintenance and production (milk and tissue) and energy efficiency during two different stages of lactation. Twenty-eight Holstein dairy cows (14 cows of each genotype) with similar calving date (May 5, 2018 ± 23 days) were managed in a pasture-based system and supplemented with one third of the predicted total dry matter intake as concentrate. Heat production, retained energy in milk and tissue, metabolizable energy intake (MEI) and the proportion of MEI retained in milk + tissue (RE/MEI) were measured at 115 and 192 ± 19 days in milk and residual HP was estimated by the difference between measured HP and predicted HP based on NRC (2001) model according to body weight, body condition score and milk production. The NAH cows were 60 ± 15 kg heavier and produced 4.7 ± 1.0 kg/d more milk with lower percentages of fat and protein than NZH cows. However, there were no differences in fat or protein yield per day between genotypes. Metabolizable energy intake, retained energy in milk and tissue, HP and RE/MEI were not different between genotypes at 115 days in milk. Nevertheless, at 192 days in milk the MEI, HP and residual HP were lower in NZH than NAH, whereas RE/MEI was not different when both genotypes were managed under a pasture-based system with one third of the consumed diet as concentrate. The capacity of NZH cows to maintain the same RE/MEI than NAH cows at 192 days in milk despite of the lower MEI, was due to a lower metabolizable energy requirement for maintenance (853 vs. 729 kJ/body weight0.75 per day for NAH and NZH, respectively). Indeed, the lower energy requirement for maintenance in NZH was associated with a lower fasting heat production since kl were not different between genotypes. Thus, NZH cows could have a lower proportion of their body as protein mass or a lower relative mass of the internal organs involved with digestive and circulatory functions. However, further investigation is necessary to understand the differences in maintenance energy requirements between the Holstein genotypes.
650 $aVACAS LECHERAS
653 $aCALORIMETRÍA INDIRECTA
653 $aDAIRY CATTLE
653 $aENERGY EXPENDTURE
653 $aGASTO DE ENERGÍA
653 $aGRAZING SYSTEM
653 $aHOLSTEIN-FRIESIAN
653 $aINDIRECT CALORIMETRY
653 $aSISTEMA DE PASTOREO
700 1 $aGARCIA-ROCHE, M
700 1 $aMENDOZA, A.
700 1 $aMATTIAUDA, D.A.
700 1 $aCARRIQUIRY, M
773 $tLivestock Science, September 2020, Volume 239, Article 104166. Doi: https://doi.org/10.1016/j.livsci.2020.104166
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