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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
21/02/2014 |
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Actualizado : |
13/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
NOYES, N.R.; WEINROTH, M.E.; PARKER, J.K.; DEAN, C.J.; LAKIN, S.M.; RAYMOND, R.A.; ROVIRA, P.J.; DOSTER, E.; ABDO, Z.; MARTIN, J.N.; JONES, K.L.; RUIZ, J.; BOUCHER, C.A.; BELK, K.E.; MORLEY, P.S. |
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Afiliación : |
NOELLE R. NOYES; MAGGIE E. WEINROTH; JENNIFER K. PARKER; CHRIS J. DEAN; STEVEN M. LAKIN; ROBERT A. RAYMOND; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE DOSTER; ZAID ABDO; JENNIFER N. MARTIN; KENNETH L. JONES; JAIME RUIZ; CHRISTINA A. BOUCHER; KEITH E. BELK; PAUL S. MORLEY. |
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Título : |
Enrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Microbiome, 2017, 5, p. 142 |
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Páginas : |
13 p. |
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DOI : |
10.1186/s40168-017-0361-8 |
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Idioma : |
Inglés |
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Notas : |
Article History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017 |
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Contenido : |
Background: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins.
Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of
microbiome-resistome dynamics. MenosBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistan... Presentar Todo |
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Palabras claves : |
ANTIMICROBIAL RESISTANCE; METAGENÓMICA; MICROBIAL ECOLOGY; MOLECULAR ENRICHMENT; RARE MICROBIOME; RESISTOME. |
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Thesagro : |
ANALISIS BIOLOGICO; ECOLOGIA MICROBIANA; RESISTENCIA A AGENTES DANINOS. |
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Asunto categoría : |
U30 Métodos de investigación |
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Marc : |
LEADER 03225naa a2200433 a 4500
001 1032862
005 2018-09-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s40168-017-0361-8$2DOI
100 1 $aNOYES, N.R.
245 $aEnrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing.$h[electronic resource]
260 $c2017
300 $a13 p.
500 $aArticle History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017
520 $aBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias. Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins. Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of microbiome-resistome dynamics.
650 $aANALISIS BIOLOGICO
650 $aECOLOGIA MICROBIANA
650 $aRESISTENCIA A AGENTES DANINOS
653 $aANTIMICROBIAL RESISTANCE
653 $aMETAGENÓMICA
653 $aMICROBIAL ECOLOGY
653 $aMOLECULAR ENRICHMENT
653 $aRARE MICROBIOME
653 $aRESISTOME
700 1 $aWEINROTH, M.E.
700 1 $aPARKER, J.K.
700 1 $aDEAN, C.J.
700 1 $aLAKIN, S.M.
700 1 $aRAYMOND, R.A.
700 1 $aROVIRA, P.J.
700 1 $aDOSTER, E.
700 1 $aABDO, Z.
700 1 $aMARTIN, J.N.
700 1 $aJONES, K.L.
700 1 $aRUIZ, J.
700 1 $aBOUCHER, C.A.
700 1 $aBELK, K.E.
700 1 $aMORLEY, P.S.
773 $tMicrobiome, 2017, 5, p. 142
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
04/03/2024 |
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Actualizado : |
04/03/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - -- |
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Autor : |
GORGOGLIONE, A.; PUPPO, L.; GAMAZO, P.; GARCIA, C.; OTERO, A. |
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Afiliación : |
ÁNGELA GORGOGLIONE, Universidad de la República, Facultad de Ingeniería, Montevideo, Uruguay; LUCÍA PUPPO, Universidad de la República, Facultad de Agronomía, Montevideo, Uruguay; PABLO GAMAZO, Universidad de la República, Centro Universitario Regional Norte, Salto, Uruguay; CLAUDIO CESAR GARCIA GALLARRETA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO RICARDO OTERO CAMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Advances in water in Agroscience. [Avances en agua en Agrociencia.]. [Avanços na água na Agrociência.]. |
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Complemento del título : |
Advances in Water in Agroscience. Editorial. Introduction. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2023, Vol.27(NE1), e1375. https://doi.org/10.31285/AGRO.27.1375 -- OPEN ACCESS. |
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ISSN : |
2730-5066 |
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DOI : |
10.31285/AGRO.27.1375 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published 6 February 2024. -- Correspondence: Angela Gorgoglione, agorgoglione@fing.edu.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/ ). |
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Contenido : |
ABSTRACT.- Worldwide, water is essential for maintaining an adequate agricultural production and fostering a sustainable ecosystem for humanity alongside diverse flora, fauna, and microbial life. However, the intensification of anthropogenic activities, the occurrence of climate variability, and the presence of environmental issues are still threatening the quantity and quality of fresh water. Therefore, integrated water management is critical for the sustainable development of a region. This Agrociencia Uruguay Special Issue on "Advances in Water in Agroscience" is designed to draw attention to new concepts, approaches, methodologies, and novel applications aiming to improve sustainable and integrated water resource management. This Special Issue contains fifteen publications sorted into the following macro fields: irrigation and water management, integrated catchment management, and water quality and environmental sustainability. -.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- A nivel mundial, el agua es esencial para mantener una producción agrícola adecuada y un ambiente sostenible para la población humana y otras formas de vida, como animales, plantas y microbios. Sin embargo, la intensificación de actividades antropogénicas, la variabilidad climática y la presencia de problemas ambientales siguen amenazando la cantidad y la calidad del agua dulce. Por tanto, la gestión integrada del agua es fundamental para el desarrollo sostenible de una región. Este número especial, titulado "Advances in Water in Agroscience" de la revista Agrociencia Uruguay, está dise-ñado para destacar nuevos conceptos, enfoques, metodologías y aplicaciones novedosas que buscan mejorar la gestión sostenible e integrada de los recursos hídricos. El número especial consta de quince publicaciones, clasificadas en tres campos principales: riego y gestión del agua, manejo integrado de cuencas, y calidad del agua y sostenibilidad ambiental. -.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMO.- Em todo o mundo, a água é essencial para manter uma produção agrícola adequada e promover um ecossistema sustentável para a humanidade, juntamente com uma diversidade de flora, fauna e vida microbiana. No entanto, a intensificação das atividades antropogênicas, a ocorrência de variabilidade climática e a presença de problemas ambientais ainda ameaçam a quantidade e qualidade da água doce. Portanto, a gestão integrada da água é fundamental para o desenvol-vimento sustentável de uma região. Esta Edição Especial da Agrociencia Uruguai sobre "Advances in Water in Agroscience" foi projetada para chamar a atenção para novos conceitos, abordagens, metodologias e aplicações inovadoras com o objetivo de melhorar a gestão sustentável e integrada dos recursos hídricos. Esta Edição Especial contém quinze pu-blicações classificadas nos seguintes campos macro: irrigação e gestão da água, gestão integrada de bacias hidrográficas e qualidade da água e sustentabilidade ambiental. .-.-. MenosABSTRACT.- Worldwide, water is essential for maintaining an adequate agricultural production and fostering a sustainable ecosystem for humanity alongside diverse flora, fauna, and microbial life. However, the intensification of anthropogenic activities, the occurrence of climate variability, and the presence of environmental issues are still threatening the quantity and quality of fresh water. Therefore, integrated water management is critical for the sustainable development of a region. This Agrociencia Uruguay Special Issue on "Advances in Water in Agroscience" is designed to draw attention to new concepts, approaches, methodologies, and novel applications aiming to improve sustainable and integrated water resource management. This Special Issue contains fifteen publications sorted into the following macro fields: irrigation and water management, integrated catchment management, and water quality and environmental sustainability. -.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- A nivel mundial, el agua es esencial para mantener una producción agrícola adecuada y un ambiente sostenible para la población humana y otras formas de vida, como animales, plantas y microbios. Sin embargo, la intensificación de actividades antropogénicas, la variabilidad climática y la presencia de problemas ambientales siguen amenazando la cantidad y la calidad del agua dulce. Por tanto, la gestión integrada del agua es fundamental para el desarrollo sostenible de una región. Este número especial, titulado ... Presentar Todo |
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Palabras claves : |
Agricultura sustentável; Basin management; Crop simulation; Gestão de bacias hidrográficas; Gestión de cuencas; Irrigação; Irrigation; Medição e controle de água; Medición y control del agua; Modelado; Modelagem; Modeling; Nutrientes en el agua; Nutrientes na água; Nutrients in water; Simulação de culturas; Simulación de cultivos; Sustainable agriculture; Water measurement and control. |
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Thesagro : |
AGRICULTURA SOSTENIBLE; RIEGO. |
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Asunto categoría : |
P10 Recursos hídricos y su ordenación |
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Marc : |
LEADER 04664naa a2200457 a 4500
001 1064486
005 2024-03-04
008 2023 bl uuuu u00u1 u #d
022 $a2730-5066
024 7 $a10.31285/AGRO.27.1375$2DOI
100 1 $aGORGOGLIONE, A.
245 $aAdvances in water in Agroscience. [Avances en agua en Agrociencia.]. [Avanços na água na Agrociência.].$h[electronic resource]
260 $c2023
500 $aArticle history: Published 6 February 2024. -- Correspondence: Angela Gorgoglione, agorgoglione@fing.edu.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/ ).
520 $aABSTRACT.- Worldwide, water is essential for maintaining an adequate agricultural production and fostering a sustainable ecosystem for humanity alongside diverse flora, fauna, and microbial life. However, the intensification of anthropogenic activities, the occurrence of climate variability, and the presence of environmental issues are still threatening the quantity and quality of fresh water. Therefore, integrated water management is critical for the sustainable development of a region. This Agrociencia Uruguay Special Issue on "Advances in Water in Agroscience" is designed to draw attention to new concepts, approaches, methodologies, and novel applications aiming to improve sustainable and integrated water resource management. This Special Issue contains fifteen publications sorted into the following macro fields: irrigation and water management, integrated catchment management, and water quality and environmental sustainability. -.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN.- A nivel mundial, el agua es esencial para mantener una producción agrícola adecuada y un ambiente sostenible para la población humana y otras formas de vida, como animales, plantas y microbios. Sin embargo, la intensificación de actividades antropogénicas, la variabilidad climática y la presencia de problemas ambientales siguen amenazando la cantidad y la calidad del agua dulce. Por tanto, la gestión integrada del agua es fundamental para el desarrollo sostenible de una región. Este número especial, titulado "Advances in Water in Agroscience" de la revista Agrociencia Uruguay, está dise-ñado para destacar nuevos conceptos, enfoques, metodologías y aplicaciones novedosas que buscan mejorar la gestión sostenible e integrada de los recursos hídricos. El número especial consta de quince publicaciones, clasificadas en tres campos principales: riego y gestión del agua, manejo integrado de cuencas, y calidad del agua y sostenibilidad ambiental. -.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMO.- Em todo o mundo, a água é essencial para manter uma produção agrícola adequada e promover um ecossistema sustentável para a humanidade, juntamente com uma diversidade de flora, fauna e vida microbiana. No entanto, a intensificação das atividades antropogênicas, a ocorrência de variabilidade climática e a presença de problemas ambientais ainda ameaçam a quantidade e qualidade da água doce. Portanto, a gestão integrada da água é fundamental para o desenvol-vimento sustentável de uma região. Esta Edição Especial da Agrociencia Uruguai sobre "Advances in Water in Agroscience" foi projetada para chamar a atenção para novos conceitos, abordagens, metodologias e aplicações inovadoras com o objetivo de melhorar a gestão sustentável e integrada dos recursos hídricos. Esta Edição Especial contém quinze pu-blicações classificadas nos seguintes campos macro: irrigação e gestão da água, gestão integrada de bacias hidrográficas e qualidade da água e sustentabilidade ambiental. .-.-.
650 $aAGRICULTURA SOSTENIBLE
650 $aRIEGO
653 $aAgricultura sustentável
653 $aBasin management
653 $aCrop simulation
653 $aGestão de bacias hidrográficas
653 $aGestión de cuencas
653 $aIrrigação
653 $aIrrigation
653 $aMedição e controle de água
653 $aMedición y control del agua
653 $aModelado
653 $aModelagem
653 $aModeling
653 $aNutrientes en el agua
653 $aNutrientes na água
653 $aNutrients in water
653 $aSimulação de culturas
653 $aSimulación de cultivos
653 $aSustainable agriculture
653 $aWater measurement and control
700 1 $aPUPPO, L.
700 1 $aGAMAZO, P.
700 1 $aGARCIA, C.
700 1 $aOTERO, A.
773 $tAgrociencia Uruguay, 2023, Vol.27(NE1), e1375. https://doi.org/10.31285/AGRO.27.1375 -- OPEN ACCESS.
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