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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
15/09/2014 |
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Actualizado : |
26/11/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
REYNO, R.; DONG-MAN, K.; MONTEROS, M.J.; BOUTON, J.H.; PARROTT, W.; BRUMMER, E.C. |
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Afiliación : |
RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DONG-MAN, KHU; MARÍA J. MONTEROS; JOSEPH H. BOUTON; WAYNE PARROTT; E. CHARLES BRUMMER. |
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Título : |
Evaluation of two transgenes for aluminum tolerance in alfalfa. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Crop Science, 2013, v. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676 |
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DOI : |
10.2135/cropsci2012.12.0676 |
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Idioma : |
Inglés |
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Notas : |
History article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot analysis, Yanling Wei for laboratory assistance with gene expressions, and Maria Ortega for helping with the RT-PCR. We would also like to thank Forage Genetics Intl. for providing plant materials R2336 and 60T180-14. This study was funded by the Georgia Agricultural Experiment Stations and The Samuel Roberts Noble Foundation. |
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Contenido : |
Alfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only,
and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the
same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and genetic backgrounds and their performance under field conditions. MenosAlfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only,
and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the
same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and ge... Presentar Todo |
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Palabras claves : |
LUCERNE. |
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Thesagro : |
ALFALFA. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02768naa a2200229 a 4500
001 1050225
005 2020-11-26
008 2013 bl uuuu u00u1 u #d
024 7 $a10.2135/cropsci2012.12.0676$2DOI
100 1 $aREYNO, R.
245 $aEvaluation of two transgenes for aluminum tolerance in alfalfa.
260 $c2013
500 $aHistory article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot analysis, Yanling Wei for laboratory assistance with gene expressions, and Maria Ortega for helping with the RT-PCR. We would also like to thank Forage Genetics Intl. for providing plant materials R2336 and 60T180-14. This study was funded by the Georgia Agricultural Experiment Stations and The Samuel Roberts Noble Foundation.
520 $aAlfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only, and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and genetic backgrounds and their performance under field conditions.
650 $aALFALFA
653 $aLUCERNE
700 1 $aDONG-MAN, K.
700 1 $aMONTEROS, M.J.
700 1 $aBOUTON, J.H.
700 1 $aPARROTT, W.
700 1 $aBRUMMER, E.C.
773 $tCrop Science, 2013$gv. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676
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INIA Tacuarembó (TBO) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
11/04/2017 |
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Actualizado : |
11/04/2017 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
DALLA RIZZA, M.; REAL, D.; REYNO, R.; QUESENBERRY, K. |
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Afiliación : |
MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL REAL FERREIRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de Western Australia (UWA); RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; K. QUESENBERRY, University of Florida (US). |
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Título : |
Use of cross-species amplification markers for pollen-mediated gene flow determination in Trifolium polymorphum Poiret. (Book Chapter) |
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Fecha de publicación : |
2005 |
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Fuente / Imprenta : |
In: Humphreys, M.O. (Ed.). Molecular Breeding for The Genetic Improvement of Forage Crops and Turf: Proceedings of the 4th International Symposium on The Molecular Breeding of Forage and Turf, a Satellite Workshop of the XXth International Grassland Congress, July 2005, Aberystwyth, Wales. |
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Páginas : |
p. 194 |
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ISBN : |
978-907699873-2 |
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DOI : |
10.3920/978-90-8686-555-0 |
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Idioma : |
Inglés |
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Palabras claves : |
COMPARATIVE GENOMICS; CROSS-SPECIES AMPLIFICATION; POPULATION BIOLOGY. |
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Thesagro : |
PASTURAS NATURALES. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 00992naa a2200229 a 4500
001 1057012
005 2017-04-11
008 2005 bl uuuu u00u1 u #d
020 $a978-907699873-2
024 7 $a10.3920/978-90-8686-555-0$2DOI
100 1 $aDALLA RIZZA, M.
245 $aUse of cross-species amplification markers for pollen-mediated gene flow determination in Trifolium polymorphum Poiret. (Book Chapter)$h[electronic resource]
260 $c2005
300 $ap. 194
650 $aPASTURAS NATURALES
653 $aCOMPARATIVE GENOMICS
653 $aCROSS-SPECIES AMPLIFICATION
653 $aPOPULATION BIOLOGY
700 1 $aREAL, D.
700 1 $aREYNO, R.
700 1 $aQUESENBERRY, K.
773 $tIn: Humphreys, M.O. (Ed.). Molecular Breeding for The Genetic Improvement of Forage Crops and Turf: Proceedings of the 4th International Symposium on The Molecular Breeding of Forage and Turf, a Satellite Workshop of the XXth International Grassland Congress, July 2005, Aberystwyth, Wales.
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