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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
30/09/2014 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ANTÚNEZ , K.; MENDOZA, Y.; SANTOS, E.; INVERNIZZI, C. |
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Afiliación : |
YAMANDU MENDOZA SPINA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Differential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Journal of Apicultural Research, v. 52, n. 5, p. 277-234, 2013. |
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ISSN : |
0021-8839. |
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DOI : |
10.3896/IBRA.1.52.5.09 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013. |
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Contenido : |
Summary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of
honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae.
Resumen:
Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una
enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas
ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N.
ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas
fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del
traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y
menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron
artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina,
mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa
deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de
vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon
abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su
expresión diferencial podría estar asociada a la resistencia a N. ceranae. MenosSummary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a cen... Presentar Todo |
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Palabras claves : |
NOSEMA CERANAE; NOSEMOSIS; PCR EN TIEMPO REAL; RESPUESTA INMUNE; VITELOGENINA. |
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Thesagro : |
APICULTURA; APIS MELLIFERA; ENFERMEDADES DE LAS ABEJAS. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 04399naa a2200289 a 4500
001 1050723
005 2019-10-11
008 2013 bl uuuu u00u1 u #d
022 $a0021-8839.
024 7 $a10.3896/IBRA.1.52.5.09$2DOI
100 1 $aANTÚNEZ , K.
245 $aDifferential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection.$h[electronic resource]
260 $c2013
500 $aArticle history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013.
520 $aSummary: Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen. The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin, hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae. Resumen: Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N. ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina, mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su expresión diferencial podría estar asociada a la resistencia a N. ceranae.
650 $aAPICULTURA
650 $aAPIS MELLIFERA
650 $aENFERMEDADES DE LAS ABEJAS
653 $aNOSEMA CERANAE
653 $aNOSEMOSIS
653 $aPCR EN TIEMPO REAL
653 $aRESPUESTA INMUNE
653 $aVITELOGENINA
700 1 $aMENDOZA, Y.
700 1 $aSANTOS, E.
700 1 $aINVERNIZZI, C.
773 $tJournal of Apicultural Research$gv. 52, n. 5, p. 277-234, 2013.
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Registro original : |
INIA La Estanzuela (LE) |
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Biblioteca
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Origen
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/09/2018 |
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Actualizado : |
12/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
AMORIM, S.T.; KLUSKA, S.; PIATTO BERTON, M.; ANTUNES DE LEMOS, M.V.; PERIPOLLI, E.; BONVINO STAFUZZA, N.; FERNÁNDEZ MARTÍN, J.; SAURA ÁLVAREZ, M.; VILLANUEVA GAVIÑA, B.; TORO, M.A.; BANCHERO, G.; SILVA OLIVEIRA, P.; GRIGOLETTO, L.; PEREIRA ELER, J.; BALDI, F.; STERMAN FERRAZ, J.B. |
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Afiliación : |
SABRINA THAISE AMORIM, Universidade Federal de Santa Catarina; SABRINA KLUSKA, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias; MARIANA PIATTO BERTON; MARCOS VINICIUS ANTUNES DE LEMOS, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias; ELISA PERIPOLLI, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias; NEDENIA BONVINO STAFUZZA, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias; JESÚS FERNÁNDEZ MARTÍN, INIA España (Instituto Nacional de Investigación y Tecnología Agraría y Alimentaria); MARÍA SAURA ÁLVAREZ, INIA España (Instituto Nacional de Investigación y Tecnología Agraría y Alimentaria); BEATRIZ VILLANUEVA GAVIÑA, INIA España (Instituto Nacional de Investigación y Tecnología Agraría y Alimentaria); MIGUEL ÁNGEL TORO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PRISCILA SILVA OLIVEIRA, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos; LAÍS GRIGOLETTO, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos; JOANIR PEREIRA ELER, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos; FERNANDO BALDI, Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias; JOSÉ BENTO STERMAN FERRAZ, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos. |
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Título : |
Genomic study for maternal related traits in Santa Inês sheep breed. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Livestock Science, November 2018, Volume 217, Pages 76-84. Doi: https://doi.org/10.1016/j.livsci.2018.09.011 |
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ISSN : |
1871-1413 |
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DOI : |
10.1016/j.livsci.2018.09.011 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 January 2018 // Revised 6 September 2018 // Accepted 11 September 2018 // Available online 20 September 2018. |
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Contenido : |
ABSTRACT.
The aim of this study was to estimate variance components and to identify genomic regions and pathways associated with maternal related traits in Santa Inês sheep breed adapted to tropical climate. Phenotypic records for maternal efficiency (ME), metabolic maternal efficiency (MME), twin lambing (TL), adult weight (AW), metabolic adult weight (MAW), and body condition score (BCS) from 1,333 ewes from Santa Inês breed were used. A total of 576 animals were genotyped with the Ovine SNP12k BeadChip (Illumina, Inc.), that contains 12,785 bialleleic SNP markers. The variance components were estimated using a single trait animal model by single step genomic BLUP procedure. For AW, MAW, BCS, ME, MME and TL the mean values were 50.30 (±9.76), 19.2 (±2.33), 2.76 (±0.72), 34.6 (±15.95), 91.8 (±42.52), and 1.27 (±0.44) respectively. The heritabilities estimated were moderate for AW (0.32) and MAW (0.33) and low for BCS (0.04), ME (0.07), MME (0.08), and TL (0.10). A total of 7, 8, 13, 16, 19, and 09 candidate regions for ME, MME, TL, AW, MAW and BCS traits were identified respectively. AW and MAW had a total of 15 regions in common, while AW and BCS had a common region on chromosome 21. ME and MME had six candidate regions in common, and TL had no common regions with any other features. The maternal indicator traits have genetic variability to respond to selection in Santa Inês breed, and it would be expected higher genetic gain for ewe adult weight when compared to the others studied traits. Several candidate regions related to growth, reproduction, lactation and enviromental adaptability were identified in this study. These candidate regions would give support to identify and select animals with higher maternal efficiency and fitness, and consequently, increase the productivity of Santa Inês sheep. Moreover, the results of this study should help to understand the genetic and physiologic mechanism associated with maternal related traits in Santa Inês breed.
© 2018 Elsevier B.V. All rights reserved. MenosABSTRACT.
The aim of this study was to estimate variance components and to identify genomic regions and pathways associated with maternal related traits in Santa Inês sheep breed adapted to tropical climate. Phenotypic records for maternal efficiency (ME), metabolic maternal efficiency (MME), twin lambing (TL), adult weight (AW), metabolic adult weight (MAW), and body condition score (BCS) from 1,333 ewes from Santa Inês breed were used. A total of 576 animals were genotyped with the Ovine SNP12k BeadChip (Illumina, Inc.), that contains 12,785 bialleleic SNP markers. The variance components were estimated using a single trait animal model by single step genomic BLUP procedure. For AW, MAW, BCS, ME, MME and TL the mean values were 50.30 (±9.76), 19.2 (±2.33), 2.76 (±0.72), 34.6 (±15.95), 91.8 (±42.52), and 1.27 (±0.44) respectively. The heritabilities estimated were moderate for AW (0.32) and MAW (0.33) and low for BCS (0.04), ME (0.07), MME (0.08), and TL (0.10). A total of 7, 8, 13, 16, 19, and 09 candidate regions for ME, MME, TL, AW, MAW and BCS traits were identified respectively. AW and MAW had a total of 15 regions in common, while AW and BCS had a common region on chromosome 21. ME and MME had six candidate regions in common, and TL had no common regions with any other features. The maternal indicator traits have genetic variability to respond to selection in Santa Inês breed, and it would be expected higher genetic gain for ewe adult weight when compared to the other... Presentar Todo |
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Palabras claves : |
BODY WEIGHT; GWAS; MATTERNAL EFFICIENCY; OVIS ARIES. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03310naa a2200385 a 4500
001 1059073
005 2021-02-12
008 2018 bl uuuu u00u1 u #d
022 $a1871-1413
024 7 $a10.1016/j.livsci.2018.09.011$2DOI
100 1 $aAMORIM, S.T.
245 $aGenomic study for maternal related traits in Santa Inês sheep breed.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 23 January 2018 // Revised 6 September 2018 // Accepted 11 September 2018 // Available online 20 September 2018.
520 $aABSTRACT. The aim of this study was to estimate variance components and to identify genomic regions and pathways associated with maternal related traits in Santa Inês sheep breed adapted to tropical climate. Phenotypic records for maternal efficiency (ME), metabolic maternal efficiency (MME), twin lambing (TL), adult weight (AW), metabolic adult weight (MAW), and body condition score (BCS) from 1,333 ewes from Santa Inês breed were used. A total of 576 animals were genotyped with the Ovine SNP12k BeadChip (Illumina, Inc.), that contains 12,785 bialleleic SNP markers. The variance components were estimated using a single trait animal model by single step genomic BLUP procedure. For AW, MAW, BCS, ME, MME and TL the mean values were 50.30 (±9.76), 19.2 (±2.33), 2.76 (±0.72), 34.6 (±15.95), 91.8 (±42.52), and 1.27 (±0.44) respectively. The heritabilities estimated were moderate for AW (0.32) and MAW (0.33) and low for BCS (0.04), ME (0.07), MME (0.08), and TL (0.10). A total of 7, 8, 13, 16, 19, and 09 candidate regions for ME, MME, TL, AW, MAW and BCS traits were identified respectively. AW and MAW had a total of 15 regions in common, while AW and BCS had a common region on chromosome 21. ME and MME had six candidate regions in common, and TL had no common regions with any other features. The maternal indicator traits have genetic variability to respond to selection in Santa Inês breed, and it would be expected higher genetic gain for ewe adult weight when compared to the others studied traits. Several candidate regions related to growth, reproduction, lactation and enviromental adaptability were identified in this study. These candidate regions would give support to identify and select animals with higher maternal efficiency and fitness, and consequently, increase the productivity of Santa Inês sheep. Moreover, the results of this study should help to understand the genetic and physiologic mechanism associated with maternal related traits in Santa Inês breed. © 2018 Elsevier B.V. All rights reserved.
653 $aBODY WEIGHT
653 $aGWAS
653 $aMATTERNAL EFFICIENCY
653 $aOVIS ARIES
700 1 $aKLUSKA, S.
700 1 $aPIATTO BERTON, M.
700 1 $aANTUNES DE LEMOS, M.V.
700 1 $aPERIPOLLI, E.
700 1 $aBONVINO STAFUZZA, N.
700 1 $aFERNÁNDEZ MARTÍN, J.
700 1 $aSAURA ÁLVAREZ, M.
700 1 $aVILLANUEVA GAVIÑA, B.
700 1 $aTORO, M.A.
700 1 $aBANCHERO, G.
700 1 $aSILVA OLIVEIRA, P.
700 1 $aGRIGOLETTO, L.
700 1 $aPEREIRA ELER, J.
700 1 $aBALDI, F.
700 1 $aSTERMAN FERRAZ, J.B.
773 $tLivestock Science, November 2018, Volume 217, Pages 76-84. Doi: https://doi.org/10.1016/j.livsci.2018.09.011
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