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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
30/09/2014 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ANTÚNEZ , K.; MENDOZA, Y.; SANTOS, E.; INVERNIZZI, C. |
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Afiliación : |
YAMANDU MENDOZA SPINA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Differential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Journal of Apicultural Research, v. 52, n. 5, p. 277-234, 2013. |
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ISSN : |
0021-8839. |
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DOI : |
10.3896/IBRA.1.52.5.09 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013. |
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Contenido : |
Summary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of
honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae.
Resumen:
Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una
enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas
ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N.
ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas
fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del
traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y
menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron
artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina,
mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa
deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de
vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon
abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su
expresión diferencial podría estar asociada a la resistencia a N. ceranae. MenosSummary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a cen... Presentar Todo |
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Palabras claves : |
NOSEMA CERANAE; NOSEMOSIS; PCR EN TIEMPO REAL; RESPUESTA INMUNE; VITELOGENINA. |
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Thesagro : |
APICULTURA; APIS MELLIFERA; ENFERMEDADES DE LAS ABEJAS. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 04399naa a2200289 a 4500
001 1050723
005 2019-10-11
008 2013 bl uuuu u00u1 u #d
022 $a0021-8839.
024 7 $a10.3896/IBRA.1.52.5.09$2DOI
100 1 $aANTÚNEZ , K.
245 $aDifferential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection.$h[electronic resource]
260 $c2013
500 $aArticle history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013.
520 $aSummary: Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen. The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin, hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae. Resumen: Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N. ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina, mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su expresión diferencial podría estar asociada a la resistencia a N. ceranae.
650 $aAPICULTURA
650 $aAPIS MELLIFERA
650 $aENFERMEDADES DE LAS ABEJAS
653 $aNOSEMA CERANAE
653 $aNOSEMOSIS
653 $aPCR EN TIEMPO REAL
653 $aRESPUESTA INMUNE
653 $aVITELOGENINA
700 1 $aMENDOZA, Y.
700 1 $aSANTOS, E.
700 1 $aINVERNIZZI, C.
773 $tJournal of Apicultural Research$gv. 52, n. 5, p. 277-234, 2013.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
02/05/2025 |
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Actualizado : |
02/05/2025 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SIRI, P.; NOGUEIRA, E.; SALVARREY, S.; BRANCHICCELA, B.; MENDOZA, Y.; BONORA, E.; INVERNIZZI, C. |
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Afiliación : |
PABLO SIRI, Departamento de Producción Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; ENRIQUE NOGUEIRA, Departamento de Producción Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; SHEENA SALVARREY, Sección Etología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0003-1624-7916; YAMANDU MENDOZA SPINA, Sección Etología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; EMILIO BONORA, Sección Etología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; CIRO INVERNIZZI, Sección Etología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Honey bees colonies in Eucalyptus grandis plantation: when the excess of nectar and pollen limits the queen's oviposition. |
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Complemento del título : |
Original research article. |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
Journal of Apicultural Research, 2025. [Latest articles]. https://doi.org/10.1080/00218839.2025.2483012 |
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ISSN : |
0021-8839 |
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DOI : |
10.1080/00218839.2025.2483012 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 19 Apr 2024, Accepted 02 Dec 2024, Published online 07 Apr 2025. -- Correspondence: Juri, P.; Departamento de Producción Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; email:pjuri8@gmail.com -- Funding: This study was partially financed by the National Agency for Research and Innovation (Project FMV_3_2016_1_125435). -- Publisher: Taylor and Francis Ltd. |
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Contenido : |
ABSTRACT.- Honey bees (Apis mellifera L.) construct a structured nest with separated areas for the brood, pollen and honey that optimizes the functioning of the colony. However, in the Eucalyptus grandis plantations, bees tend to store a great amount of pollen and nectar in the brood area during the flowering period, reducing the space for the queen's egg laying. Through the python informatic program "CombCount" and an image program, the capped brood area and the proportion of cells occupied by brood, pollen or nectar during the flowering period of E. grandis in colonies with and without polyfloral pollen supplement was determined. In the middle of the flowering period of E. grandis, in just 9 days, a decrease of almost 40% of the total amount of capped brood was recorded. In this period in the brood area the cells occupied by brood decrease to values under 62% while the remaining cells are occupied mostly by pollen or nectar. The supplementation with polyfloral pollen did not affect these variations. At the end of the flowering period of E. grandis the colonies that received polyfloral pollen supplements had a population of bees 25% higher than the colonies without supplementation, although this difference was not reflected in the production of honey. This study shows that, paradoxically, an abundant offer of pollen and nectar during a brief period can limit the egg laying of the queen and have consequences on the bee population. © 2025 International Bee Research Association. MenosABSTRACT.- Honey bees (Apis mellifera L.) construct a structured nest with separated areas for the brood, pollen and honey that optimizes the functioning of the colony. However, in the Eucalyptus grandis plantations, bees tend to store a great amount of pollen and nectar in the brood area during the flowering period, reducing the space for the queen's egg laying. Through the python informatic program "CombCount" and an image program, the capped brood area and the proportion of cells occupied by brood, pollen or nectar during the flowering period of E. grandis in colonies with and without polyfloral pollen supplement was determined. In the middle of the flowering period of E. grandis, in just 9 days, a decrease of almost 40% of the total amount of capped brood was recorded. In this period in the brood area the cells occupied by brood decrease to values under 62% while the remaining cells are occupied mostly by pollen or nectar. The supplementation with polyfloral pollen did not affect these variations. At the end of the flowering period of E. grandis the colonies that received polyfloral pollen supplements had a population of bees 25% higher than the colonies without supplementation, although this difference was not reflected in the production of honey. This study shows that, paradoxically, an abundant offer of pollen and nectar during a brief period can limit the egg laying of the queen and have consequences on the bee population. © 2025 International Bee Research Associatio... Presentar Todo |
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Palabras claves : |
APICULTURA - INIA; Brood; Cells; Honey bees behavior; Nest organization; Queen laying. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02838naa a2200301 a 4500
001 1065177
005 2025-05-02
008 2025 bl uuuu u00u1 u #d
022 $a0021-8839
024 7 $a10.1080/00218839.2025.2483012$2DOI
100 1 $aSIRI, P.
245 $aHoney bees colonies in Eucalyptus grandis plantation$bwhen the excess of nectar and pollen limits the queen's oviposition.$h[electronic resource]
260 $c2025
500 $aArticle history: Received 19 Apr 2024, Accepted 02 Dec 2024, Published online 07 Apr 2025. -- Correspondence: Juri, P.; Departamento de Producción Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; email:pjuri8@gmail.com -- Funding: This study was partially financed by the National Agency for Research and Innovation (Project FMV_3_2016_1_125435). -- Publisher: Taylor and Francis Ltd.
520 $aABSTRACT.- Honey bees (Apis mellifera L.) construct a structured nest with separated areas for the brood, pollen and honey that optimizes the functioning of the colony. However, in the Eucalyptus grandis plantations, bees tend to store a great amount of pollen and nectar in the brood area during the flowering period, reducing the space for the queen's egg laying. Through the python informatic program "CombCount" and an image program, the capped brood area and the proportion of cells occupied by brood, pollen or nectar during the flowering period of E. grandis in colonies with and without polyfloral pollen supplement was determined. In the middle of the flowering period of E. grandis, in just 9 days, a decrease of almost 40% of the total amount of capped brood was recorded. In this period in the brood area the cells occupied by brood decrease to values under 62% while the remaining cells are occupied mostly by pollen or nectar. The supplementation with polyfloral pollen did not affect these variations. At the end of the flowering period of E. grandis the colonies that received polyfloral pollen supplements had a population of bees 25% higher than the colonies without supplementation, although this difference was not reflected in the production of honey. This study shows that, paradoxically, an abundant offer of pollen and nectar during a brief period can limit the egg laying of the queen and have consequences on the bee population. © 2025 International Bee Research Association.
653 $aAPICULTURA - INIA
653 $aBrood
653 $aCells
653 $aHoney bees behavior
653 $aNest organization
653 $aQueen laying
700 1 $aNOGUEIRA, E.
700 1 $aSALVARREY, S.
700 1 $aBRANCHICCELA, B.
700 1 $aMENDOZA, Y.
700 1 $aBONORA, E.
700 1 $aINVERNIZZI, C.
773 $tJournal of Apicultural Research, 2025. [Latest articles]. https://doi.org/10.1080/00218839.2025.2483012
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