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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
08/06/2015 |
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Actualizado : |
13/05/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DE BARBIERI, I.; GULINO, L.; HEGARTY, R.S.; ODDY, V.H.; MAQUIRE, A.; LI, L.; KLIEVE, A.V.; OUWERKERK, D. |
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Afiliación : |
LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LISA MAREE GULINO, Rumen Ecology Unit, Queensland Department of Agriculture, Australia; Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD, Australia; ROGER STEPHEN HEGARTY, a School of Environmental and Rural Science, University of New England, Armidale, NSW, Australia; VICTOR ODDY, Beef Industry Centre, Department of Primary Industries NSW, Armidale, NSW, Australia; ANITA J. MAGUIRE, Rumen Ecology Unit, Queensland Department of Agriculture, Fisheries and Forestry, Brisbane, QLD, Australia; L. LI, School of Environmental and Rural Science, University of New England, Armidale, NSW, Australia; ATHOL V. KLIEVE, Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD, Australia; School of Agriculture and Food Sciences, University of Queensland, Gatton, QLD, Australia; DIANE J. OUWERKERK, Rumen Ecology Unit, Queensland Department of Agriculture, Fisheries and Forestry, Brisbane, QLD, Australia; Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD, Australia. |
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Título : |
Production attributes of Merino sheep genetically divergent for wool growth are reflected in differing rumen microbiotas. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Livestock Science, Volume 178, August 2015, Pages 119-129. DOI: https://doi.org/10.1016/j.livsci.2015.05.023 |
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DOI : |
10.1016/j.livsci.2015.05.023 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 13 October 2014; Received in revised form 7 May 2015; Accepted 17 May 2015. Acknowledgments: The authors thank Alistair Donaldson, Reginald Woodgate, Gary Taylor, Damien Finn, and Ros Gilbert for their collaboration. Ignacio De Barbieri was supported by National Institute for Agricultural Research (INIA Uruguay). Financial support for this project was also provided by the Australian Government's the Rumen Pangenome project within Filling the Research Gap (FTRG-1194147-75) program. |
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Contenido : |
Divergent genetic selection for wool growth as a single trait has led to major changes in sheep physiology and metabolism, including variations in rumen microbial protein production and uptake of ?-amino nitrogen in portal blood. This study was conducted to determine if sheep with different genetic merit for wool growth exhibit distinct rumen bacterial diversity. Eighteen Merino wethers were separated into groups of contrasting genetic merit for clean fleece weight (CFW; low: WG? and high: WG+) and fed a blend of oaten and lucerne chaff diet at two levels of intake (LOI; 1 or 1.5 times maintenance energy requirements) for two seven-week periods in a crossover design. Bacterial diversity in rumen fluid collected by esophageal intubation was characterized using 454 amplicon pyrosequencing of the V3/V4 regions of the 16S rRNA gene. Bacterial diversity estimated by Phylogenetic distance, Chao1 and observed species did not differ significantly with CFW or LOI; however, the Shannon diversity index differed (P=0.04) between WG+ (7.67) and WG? sheep (8.02). WG+ animals had a higher (P=0.03) proportion of Bacteroidetes (71.9% vs 66.5%) and a lower (P=0.04) proportion of Firmicutes (26.6% vs 31.6%) than WG? animals. Twenty-four specific operational taxonomic units (OTUs), belonging to the Firmicutes and Bacteroidetes phyla, were shared among all the samples, whereas specific OTUs varied significantly in presence/abundance (P<0.05) between wool genotypes and 50 varied (P<0.05) with LOI. It appears that genetic selection for fleece weight is associated with differences in rumen bacterial diversity that persist across different feeding levels. Moderate correlations between seven continuous traits, such as methane production or microbial protein production, and the presence and abundance of 17 OTUs were found, indicating scope for targeted modification of the microbiome to improve the energetic efficiency of rumen microbial synthesis and reduce the greenhouse gas footprint of ruminants. MenosDivergent genetic selection for wool growth as a single trait has led to major changes in sheep physiology and metabolism, including variations in rumen microbial protein production and uptake of ?-amino nitrogen in portal blood. This study was conducted to determine if sheep with different genetic merit for wool growth exhibit distinct rumen bacterial diversity. Eighteen Merino wethers were separated into groups of contrasting genetic merit for clean fleece weight (CFW; low: WG? and high: WG+) and fed a blend of oaten and lucerne chaff diet at two levels of intake (LOI; 1 or 1.5 times maintenance energy requirements) for two seven-week periods in a crossover design. Bacterial diversity in rumen fluid collected by esophageal intubation was characterized using 454 amplicon pyrosequencing of the V3/V4 regions of the 16S rRNA gene. Bacterial diversity estimated by Phylogenetic distance, Chao1 and observed species did not differ significantly with CFW or LOI; however, the Shannon diversity index differed (P=0.04) between WG+ (7.67) and WG? sheep (8.02). WG+ animals had a higher (P=0.03) proportion of Bacteroidetes (71.9% vs 66.5%) and a lower (P=0.04) proportion of Firmicutes (26.6% vs 31.6%) than WG? animals. Twenty-four specific operational taxonomic units (OTUs), belonging to the Firmicutes and Bacteroidetes phyla, were shared among all the samples, whereas specific OTUs varied significantly in presence/abundance (P<0.05) between wool genotypes and 50 varied (P<0.05) with LOI... Presentar Todo |
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Palabras claves : |
BACTERIAL COMMUNITIES; LEVEL OF INTAKE; QIIME; RUMEN ECOLOGY; WOOL GENOTYPTE. |
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Thesagro : |
GENOTIPOS; LANA; MERINO; OVINOS. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 03480naa a2200337 a 4500
001 1052743
005 2020-05-13
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.livsci.2015.05.023$2DOI
100 1 $aDE BARBIERI, I.
245 $aProduction attributes of Merino sheep genetically divergent for wool growth are reflected in differing rumen microbiotas.
260 $c2015
500 $aArticle history: Received 13 October 2014; Received in revised form 7 May 2015; Accepted 17 May 2015. Acknowledgments: The authors thank Alistair Donaldson, Reginald Woodgate, Gary Taylor, Damien Finn, and Ros Gilbert for their collaboration. Ignacio De Barbieri was supported by National Institute for Agricultural Research (INIA Uruguay). Financial support for this project was also provided by the Australian Government's the Rumen Pangenome project within Filling the Research Gap (FTRG-1194147-75) program.
520 $aDivergent genetic selection for wool growth as a single trait has led to major changes in sheep physiology and metabolism, including variations in rumen microbial protein production and uptake of ?-amino nitrogen in portal blood. This study was conducted to determine if sheep with different genetic merit for wool growth exhibit distinct rumen bacterial diversity. Eighteen Merino wethers were separated into groups of contrasting genetic merit for clean fleece weight (CFW; low: WG? and high: WG+) and fed a blend of oaten and lucerne chaff diet at two levels of intake (LOI; 1 or 1.5 times maintenance energy requirements) for two seven-week periods in a crossover design. Bacterial diversity in rumen fluid collected by esophageal intubation was characterized using 454 amplicon pyrosequencing of the V3/V4 regions of the 16S rRNA gene. Bacterial diversity estimated by Phylogenetic distance, Chao1 and observed species did not differ significantly with CFW or LOI; however, the Shannon diversity index differed (P=0.04) between WG+ (7.67) and WG? sheep (8.02). WG+ animals had a higher (P=0.03) proportion of Bacteroidetes (71.9% vs 66.5%) and a lower (P=0.04) proportion of Firmicutes (26.6% vs 31.6%) than WG? animals. Twenty-four specific operational taxonomic units (OTUs), belonging to the Firmicutes and Bacteroidetes phyla, were shared among all the samples, whereas specific OTUs varied significantly in presence/abundance (P<0.05) between wool genotypes and 50 varied (P<0.05) with LOI. It appears that genetic selection for fleece weight is associated with differences in rumen bacterial diversity that persist across different feeding levels. Moderate correlations between seven continuous traits, such as methane production or microbial protein production, and the presence and abundance of 17 OTUs were found, indicating scope for targeted modification of the microbiome to improve the energetic efficiency of rumen microbial synthesis and reduce the greenhouse gas footprint of ruminants.
650 $aGENOTIPOS
650 $aLANA
650 $aMERINO
650 $aOVINOS
653 $aBACTERIAL COMMUNITIES
653 $aLEVEL OF INTAKE
653 $aQIIME
653 $aRUMEN ECOLOGY
653 $aWOOL GENOTYPTE
700 1 $aGULINO, L.
700 1 $aHEGARTY, R.S.
700 1 $aODDY, V.H.
700 1 $aMAQUIRE, A.
700 1 $aLI, L.
700 1 $aKLIEVE, A.V.
700 1 $aOUWERKERK, D.
773 $tLivestock Science, Volume 178, August 2015, Pages 119-129. DOI: https://doi.org/10.1016/j.livsci.2015.05.023
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| 15. |  | LUSSICH, N.; ELHORDOY, J.A.; CHOUY, J. Investigar para crecer. El País Agropecuario, 1998, v. 4, no. 46, p. 13-26. Contiene además: La organización del INIA: En sintonía con los sectores productivos. Con el presidente del INIA, Ing. Agr. Pedro Bonino: El conocimiento tecnológico no existe hasta el momento en que se adopta. El INIA y sus Estaciones...| Biblioteca(s): INIA Tacuarembó. |
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