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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
06/05/2016 |
Actualizado : |
23/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
MAYA, L.; PUENTES, R.; REOLÓN, E.; ACUÑA, P.; RIET-CORREA, F.; RIVERO, R.; CRISTINA, J.; COLINA, R. |
Afiliación : |
L. MAYA, Laboratorio de Virología Molecular, Regional Norte, CENUR Noroeste, Universidad de la República; R. PUENTES, Área de Inmunología, Facultad de Veterinaria, Universidad de la República; E. REOLÓN, Laboratorios Santa Elena-VIRBAC; P. ACUÑA, Laboratorios Santa Elena-VIRBAC; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; R. RIVERO, DILAVE “Miguel C. Rubino”, Laboratorio Regional Noroeste; J. CRISTINA, Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República; R. COLINA, Laboratorio de Virología Molecular, Regional Norte, CENUR Noroeste, Universidad de la República. |
Título : |
Molecular diversity of bovine viral diarrhea virus in Uruguay. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Archives of Virology, 2016, v.161, no.3, p. 529-535. |
DOI : |
10.1007/s00705-015-2688-4 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 1 July 2015 / Accepted: 14 November 2015 / Published online: 23 November 2015 |
Contenido : |
ABSTRACT.
Bovine viral diarrhea (BVD) affects bovine production and reproduction causing significant economic losses all over the world. Two viral species has been recognized: BVDV-1 and BVDV-2, both distributed worldwide. Recently, novel specie of BVDV named HoBi-like pestivirus was discovered. The presence of BVDV was confirmed in 1996 in Uruguay, however, does not exist until today a schedule of compulsory vaccination along the country. Serological studies with samples from all Uruguayan herds were performed during 2000 and 2001 demonstrating that all of them were seropositive to BVDV with a mean prevalence of 69 %. In addition, there have been no new studies done since those previously described and it is important to mention that the genetic diversity of BVD has never been described in Uruguay. Nowadays, there is strongly suspect that BVDV is one of the most important causes of reproductive failures in our herds. The aim of this study was to describe for the first time in Uruguay the genetic diversity of BVDV with samples collected from different regions along the country. Serological status of 390 non-vaccinated animals against BVDV with reproductive problems from farms of Rivera, Tacuarembó and Florida departments of Uruguay were studied. All herds were seropositive to BVDV and high proportion of animals were positive (298/390), while 4.1 % (16/390) of the animals were positive to Antigen Capture ELISA test and Real Time PCR. Phylogenetic analysis performed with concatenated sequences from the 5′UTR and Npro genomic regions revealed that BVDV-1 and BVDV-2 are infecting our herds, being BVDV-1 the most frequently found. The major subtype was BVDV-1a, followed by BVDV-1i and BVDV-2b. This is the first study that describes the genetic diversity of BVDV in Uruguay and it will contribute to the elaboration of sanitization programs.
@ Springer-Verlag Wien 2015 MenosABSTRACT.
Bovine viral diarrhea (BVD) affects bovine production and reproduction causing significant economic losses all over the world. Two viral species has been recognized: BVDV-1 and BVDV-2, both distributed worldwide. Recently, novel specie of BVDV named HoBi-like pestivirus was discovered. The presence of BVDV was confirmed in 1996 in Uruguay, however, does not exist until today a schedule of compulsory vaccination along the country. Serological studies with samples from all Uruguayan herds were performed during 2000 and 2001 demonstrating that all of them were seropositive to BVDV with a mean prevalence of 69 %. In addition, there have been no new studies done since those previously described and it is important to mention that the genetic diversity of BVD has never been described in Uruguay. Nowadays, there is strongly suspect that BVDV is one of the most important causes of reproductive failures in our herds. The aim of this study was to describe for the first time in Uruguay the genetic diversity of BVDV with samples collected from different regions along the country. Serological status of 390 non-vaccinated animals against BVDV with reproductive problems from farms of Rivera, Tacuarembó and Florida departments of Uruguay were studied. All herds were seropositive to BVDV and high proportion of animals were positive (298/390), while 4.1 % (16/390) of the animals were positive to Antigen Capture ELISA test and Real Time PCR. Phylogenetic analysis performed with conc... Presentar Todo |
Palabras claves : |
BOVINE VIRAL DIARRHEA (BVD). |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES; VIROLOGIA. |
Asunto categoría : |
-- |
Marc : |
LEADER 02738naa a2200265 a 4500 001 1054797 005 2019-10-23 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1007/s00705-015-2688-4$2DOI 100 1 $aMAYA, L. 245 $aMolecular diversity of bovine viral diarrhea virus in Uruguay.$h[electronic resource] 260 $c2016 500 $aArticle history: Received: 1 July 2015 / Accepted: 14 November 2015 / Published online: 23 November 2015 520 $aABSTRACT. Bovine viral diarrhea (BVD) affects bovine production and reproduction causing significant economic losses all over the world. Two viral species has been recognized: BVDV-1 and BVDV-2, both distributed worldwide. Recently, novel specie of BVDV named HoBi-like pestivirus was discovered. The presence of BVDV was confirmed in 1996 in Uruguay, however, does not exist until today a schedule of compulsory vaccination along the country. Serological studies with samples from all Uruguayan herds were performed during 2000 and 2001 demonstrating that all of them were seropositive to BVDV with a mean prevalence of 69 %. In addition, there have been no new studies done since those previously described and it is important to mention that the genetic diversity of BVD has never been described in Uruguay. Nowadays, there is strongly suspect that BVDV is one of the most important causes of reproductive failures in our herds. The aim of this study was to describe for the first time in Uruguay the genetic diversity of BVDV with samples collected from different regions along the country. Serological status of 390 non-vaccinated animals against BVDV with reproductive problems from farms of Rivera, Tacuarembó and Florida departments of Uruguay were studied. All herds were seropositive to BVDV and high proportion of animals were positive (298/390), while 4.1 % (16/390) of the animals were positive to Antigen Capture ELISA test and Real Time PCR. Phylogenetic analysis performed with concatenated sequences from the 5′UTR and Npro genomic regions revealed that BVDV-1 and BVDV-2 are infecting our herds, being BVDV-1 the most frequently found. The major subtype was BVDV-1a, followed by BVDV-1i and BVDV-2b. This is the first study that describes the genetic diversity of BVDV in Uruguay and it will contribute to the elaboration of sanitization programs. @ Springer-Verlag Wien 2015 650 $aENFERMEDADES DE LOS ANIMALES 650 $aVIROLOGIA 653 $aBOVINE VIRAL DIARRHEA (BVD) 700 1 $aPUENTES, R. 700 1 $aREOLÓN, E. 700 1 $aACUÑA, P. 700 1 $aRIET-CORREA, F. 700 1 $aRIVERO, R. 700 1 $aCRISTINA, J. 700 1 $aCOLINA, R. 773 $tArchives of Virology, 2016$gv.161, no.3, p. 529-535.
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
10/09/2014 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
B - 1 |
Autor : |
LEONI, C.; DE VRIES, M.; TER BRAAK, C.J.F.; VAN BRUGGEN, A.H.C.; ROSSING, W.A.H. |
Afiliación : |
CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Fusarium oxysporum f.sp. cepae dynamics: In-plant multiplication and crop sequence simulations. |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
European Journal of Plant Pathology, 2013, v.137, no.3, p.545-561. |
ISSN : |
0929-1873 |
DOI : |
10.1007/s10658-013-0268-6 |
Idioma : |
Inglés |
Notas : |
Article history: Accepted: 1 August 2013 / Published online: 23 August 2013. |
Contenido : |
ABSTRACT.
To reduce Fusarium Basal Rot caused by Fusarium oxysporum f.sp. cepae (Foc) through crop rotation, plant species should be selected based on Foc multiplication in their roots. Foc multiplication rates in 13 plant species were tested in a greenhouse. All plant species enabled Foc multiplication. The lowest Foc levels (cfu g−1 dry root) were found for wheat, sunflower, cowpea and millet, the highest for black bean. The highest Foc levels per plant were calculated for sudan grass. These data were used to calibrate the model Pf=Pi/(α+βPi) relating final (Pf) and initial (Pi) Foc levels in the soil. The rate of population increase at low Pi (1/α) was highest for onion and black oat and smallest for sunflower. The pathogen carrying capacity (1/β) was highest for black oat and black bean, and lowest forwheat, cowpea and foxtail millet. Foc soil population dynamics was simulated for crop sequences by concatenating Pi-Pf values, considering instantaneous or gradual pathogen release after harvest. Different soil Foc populations were attained after reaching steady states. Foc populations in the sequence onion ?foxtail millet - wheat ? cowpea were 67 % lower than in the sequence onion ? sudan grass - black oat - black beans. In this work, by combining detailed greenhouse experiments with modelling, we were able to screen crops for their ability to increase Foc population and to explore potential crop sequences that may limit pathogen build-up.
© 2013 KNPV. MenosABSTRACT.
To reduce Fusarium Basal Rot caused by Fusarium oxysporum f.sp. cepae (Foc) through crop rotation, plant species should be selected based on Foc multiplication in their roots. Foc multiplication rates in 13 plant species were tested in a greenhouse. All plant species enabled Foc multiplication. The lowest Foc levels (cfu g−1 dry root) were found for wheat, sunflower, cowpea and millet, the highest for black bean. The highest Foc levels per plant were calculated for sudan grass. These data were used to calibrate the model Pf=Pi/(α+βPi) relating final (Pf) and initial (Pi) Foc levels in the soil. The rate of population increase at low Pi (1/α) was highest for onion and black oat and smallest for sunflower. The pathogen carrying capacity (1/β) was highest for black oat and black bean, and lowest forwheat, cowpea and foxtail millet. Foc soil population dynamics was simulated for crop sequences by concatenating Pi-Pf values, considering instantaneous or gradual pathogen release after harvest. Different soil Foc populations were attained after reaching steady states. Foc populations in the sequence onion ?foxtail millet - wheat ? cowpea were 67 % lower than in the sequence onion ? sudan grass - black oat - black beans. In this work, by combining detailed greenhouse experiments with modelling, we were able to screen crops for their ability to increase Foc population and to explore potential crop sequences that may limit pathogen build-up.
© 2013... Presentar Todo |
Thesagro : |
DINÁMICA DE POBLACIONES; FUSARIUM; MODELOS DE SIMULACIÓN; RELACIONES HUÉSPED-PARÁSITO. |
Asunto categoría : |
F40 Ecología vegetal |
Marc : |
LEADER 02358naa a2200253 a 4500 001 1050132 005 2019-10-15 008 2013 bl uuuu u00u1 u #d 022 $a0929-1873 024 7 $a10.1007/s10658-013-0268-6$2DOI 100 1 $aLEONI, C. 245 $aFusarium oxysporum f.sp. cepae dynamics$bIn-plant multiplication and crop sequence simulations.$h[electronic resource] 260 $c2013 500 $aArticle history: Accepted: 1 August 2013 / Published online: 23 August 2013. 520 $aABSTRACT. To reduce Fusarium Basal Rot caused by Fusarium oxysporum f.sp. cepae (Foc) through crop rotation, plant species should be selected based on Foc multiplication in their roots. Foc multiplication rates in 13 plant species were tested in a greenhouse. All plant species enabled Foc multiplication. The lowest Foc levels (cfu g−1 dry root) were found for wheat, sunflower, cowpea and millet, the highest for black bean. The highest Foc levels per plant were calculated for sudan grass. These data were used to calibrate the model Pf=Pi/(α+βPi) relating final (Pf) and initial (Pi) Foc levels in the soil. The rate of population increase at low Pi (1/α) was highest for onion and black oat and smallest for sunflower. The pathogen carrying capacity (1/β) was highest for black oat and black bean, and lowest forwheat, cowpea and foxtail millet. Foc soil population dynamics was simulated for crop sequences by concatenating Pi-Pf values, considering instantaneous or gradual pathogen release after harvest. Different soil Foc populations were attained after reaching steady states. Foc populations in the sequence onion ?foxtail millet - wheat ? cowpea were 67 % lower than in the sequence onion ? sudan grass - black oat - black beans. In this work, by combining detailed greenhouse experiments with modelling, we were able to screen crops for their ability to increase Foc population and to explore potential crop sequences that may limit pathogen build-up. © 2013 KNPV. 650 $aDINÁMICA DE POBLACIONES 650 $aFUSARIUM 650 $aMODELOS DE SIMULACIÓN 650 $aRELACIONES HUÉSPED-PARÁSITO 700 1 $aDE VRIES, M. 700 1 $aTER BRAAK, C.J.F. 700 1 $aVAN BRUGGEN, A.H.C. 700 1 $aROSSING, W.A.H. 773 $tEuropean Journal of Plant Pathology, 2013$gv.137, no.3, p.545-561.
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