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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
09/08/2016 |
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Actualizado : |
31/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SILVA, L.C.A.; PESSOA, D.A.N.; LOPES, J.R.G.; DE ALBUQUERQUE, L.G.; SILVA, L.S.A.; JUNIOR, F.G.; RIET-CORREA, F. |
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Afiliación : |
FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.// Veterinary Hospital Federal University of Campina Grande, Patos, Paraiba, CEP 58700-000, Brazil. |
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Título : |
Protection against Amorimia septentrionalis poisoning in goats by the continuous administration of sodium monofluoroacetate-degrading bacteria. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Toxicon, v. 111, p. 65-68. 2016. |
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DOI : |
10.1016/j.toxicon.2015.12.016 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 October 2015//Received in revised form 22 December 2015//Accepted 28 December 2015//Available online 30 December 2015. |
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Palabras claves : |
BURKHOLDERIA SP; CUPRIAVIDUS SP; LA MUERTE SÚBITA; MONOFLUOROACETATO DE SODIO; PAENIBACILLUS SP; RALSTONIA SP; SALUD ANIMAL; SODIUM MONOFLUOROACETATE; SUDDEN DEATH. |
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Thesagro : |
CABRAS; ENVENENAMIENTO. |
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Asunto categoría : |
L74 Trastornos misceláneos de los animales |
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Marc : |
LEADER 01163naa a2200337 a 4500
001 1055215
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008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.toxicon.2015.12.016$2DOI
100 1 $aSILVA, L.C.A.
245 $aProtection against Amorimia septentrionalis poisoning in goats by the continuous administration of sodium monofluoroacetate-degrading bacteria.$h[electronic resource]
260 $c2016
500 $aArticle history: Received 9 October 2015//Received in revised form 22 December 2015//Accepted 28 December 2015//Available online 30 December 2015.
650 $aCABRAS
650 $aENVENENAMIENTO
653 $aBURKHOLDERIA SP
653 $aCUPRIAVIDUS SP
653 $aLA MUERTE SÚBITA
653 $aMONOFLUOROACETATO DE SODIO
653 $aPAENIBACILLUS SP
653 $aRALSTONIA SP
653 $aSALUD ANIMAL
653 $aSODIUM MONOFLUOROACETATE
653 $aSUDDEN DEATH
700 1 $aPESSOA, D.A.N.
700 1 $aLOPES, J.R.G.
700 1 $aDE ALBUQUERQUE, L.G.
700 1 $aSILVA, L.S.A.
700 1 $aJUNIOR, F.G.
700 1 $aRIET-CORREA, F.
773 $tToxicon$gv. 111, p. 65-68. 2016.
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Registro original : |
INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
09/04/2025 |
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Actualizado : |
09/04/2025 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SCHVARTZMAN, C.; MURCHIO, S.; CASTRO, A.; CIBILS-STEWART, X.; MIRANDA, P.; MUJICA, V.; PARDO, H.; DALLA RIZZA, M. |
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Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANALÍA CASTRO, Centro NanoMat, DETEMA, Instituto Polo Tecnológico de Pando, Facultad de Química, Universidad de la República, Pando, Uruguay; XIMENA CIBILS-STEWART, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0003-0296-5554; PABLO MIRANDA, Centro NanoMat, DETEMA, Instituto Polo Tecnológico de Pando, Facultad de Química, Universidad de la República, Pando, Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-9820-2879; HELENA PARDO, Centro NanoMat, DETEMA, Instituto Polo Tecnológico de Pando, Facultad de Química, Universidad de la República, Pando, Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0003-4065-0940. |
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Título : |
Chitosan double-stranded RNA nanocomplexes for Piezodorus guildinii control. [Research article]. |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
Pest Management Science, 2025. [Early view]. https://doi.org/10.1002/ps.8744 |
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ISSN : |
1526-498X |
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DOI : |
10.1002/ps.8744 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 September 2024, Revised 7 January 2025, First published 05 March 2025. -- Correspondence: Schvartzman, C.; email:schvartzman@inia.org.uy -- Funding: This work was funded by the FSA_1_2018_1_151855 Innovagro grant from Agencia Nacional de Investigación e Innovación
(ANII), and by the Instituto Nacional de Investivgación Agropecuaria (INIA) BT_20 grant. -- Publisher: John Wiley and Sons Ltd. -- |
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Contenido : |
ABSTRACT.- BACKGROUND: Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated by double-stranded RNA (dsRNA), may be used as a safe technique in integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect dsRNA from degradation has shown to enhance this response. This study aims to assess the impact of dsRNA administration on the viability of P. guildinii and evaluate dsRNA-chitosan nanocomplexes as a pest control strategy. RESULTS: Ten target genes were selected, dsRNAs were designed and synthesized. In injection assays, significant mortality exceeding 76% was observed, while mortality from ingestion also showed significant differences, reaching 49%. The dsRNA targeting Srp54k gene was selected and a chitosan-dsRNA nanocomplex was synthesized. These nanocomplexes demonstrated enhanced stability under RNase treatment in vitro, and, in in vivo assays, a cumulative corrected mortality rate of 66% was reported. Consistent with these findings, RT-qPCR analysis revealed that gene expression levels in treated insects were significantly reduced compared to control at 24, 48, and 72 h post treatment for targeted genes. CONCLUSION: In this work, we evaluated the effect of dsRNA administration by injection and ingestion on the viability of P. guildinii and determined its molecular silencing effects. Additionally, we synthesized and characterized chitosan-dsRNA nanoparticles, which demonstrated improved efficacy in terms of stability. These findings are promising for the development of new integrated management strategies for stink bugs control. © 2025 Society of Chemical Industry. © 2025 Society of Chemical Industry. MenosABSTRACT.- BACKGROUND: Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated by double-stranded RNA (dsRNA), may be used as a safe technique in integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect dsRNA from degradation has shown to enhance this response. This study aims to assess the impact of dsRNA administration on the viability of P. guildinii and evaluate dsRNA-chitosan nanocomplexes as a pest control strategy. RESULTS: Ten target genes were selected, dsRNAs were designed and synthesized. In injection assays, significant mortality exceeding 76% was observed, while mortality from ingestion also showed significant differences, reaching 49%. The dsRNA targeting Srp54k gene was selected and a chitosan-dsRNA nanocomplex was synthesized. These nanocomplexes demonstrated enhanced stability under RNase treatment in vitro, and, in in vivo assays, a cumulative corrected mortality rate of 66% was reported. Consistent with these findings, RT-qPCR analysis revealed that gene expression levels in treated insects were significantly reduced compared to control at 24, 48, and 72 h post treatment for targeted genes. CONCLUSION: In this work, we evaluated the effect of dsRNA administration by injection and ingestion on the viability of P. guildinii a... Presentar Todo |
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Palabras claves : |
ÁREA DE MEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA; Gene silencing; Hemiptera; Pest control; RNAi; Stink bugs; Sustainable Development Goals (SDGs); Zero hunger - Goal 2. |
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Asunto categoría : |
H10 Plagas de las plantas |
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Marc : |
LEADER 03306naa a2200337 a 4500
001 1065164
005 2025-04-09
008 2025 bl uuuu u00u1 u #d
022 $a1526-498X
024 7 $a10.1002/ps.8744$2DOI
100 1 $aSCHVARTZMAN, C.
245 $aChitosan double-stranded RNA nanocomplexes for Piezodorus guildinii control. [Research article].$h[electronic resource]
260 $c2025
500 $aArticle history: Received 30 September 2024, Revised 7 January 2025, First published 05 March 2025. -- Correspondence: Schvartzman, C.; email:schvartzman@inia.org.uy -- Funding: This work was funded by the FSA_1_2018_1_151855 Innovagro grant from Agencia Nacional de Investigación e Innovación (ANII), and by the Instituto Nacional de Investivgación Agropecuaria (INIA) BT_20 grant. -- Publisher: John Wiley and Sons Ltd. --
520 $aABSTRACT.- BACKGROUND: Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated by double-stranded RNA (dsRNA), may be used as a safe technique in integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect dsRNA from degradation has shown to enhance this response. This study aims to assess the impact of dsRNA administration on the viability of P. guildinii and evaluate dsRNA-chitosan nanocomplexes as a pest control strategy. RESULTS: Ten target genes were selected, dsRNAs were designed and synthesized. In injection assays, significant mortality exceeding 76% was observed, while mortality from ingestion also showed significant differences, reaching 49%. The dsRNA targeting Srp54k gene was selected and a chitosan-dsRNA nanocomplex was synthesized. These nanocomplexes demonstrated enhanced stability under RNase treatment in vitro, and, in in vivo assays, a cumulative corrected mortality rate of 66% was reported. Consistent with these findings, RT-qPCR analysis revealed that gene expression levels in treated insects were significantly reduced compared to control at 24, 48, and 72 h post treatment for targeted genes. CONCLUSION: In this work, we evaluated the effect of dsRNA administration by injection and ingestion on the viability of P. guildinii and determined its molecular silencing effects. Additionally, we synthesized and characterized chitosan-dsRNA nanoparticles, which demonstrated improved efficacy in terms of stability. These findings are promising for the development of new integrated management strategies for stink bugs control. © 2025 Society of Chemical Industry. © 2025 Society of Chemical Industry.
653 $aÁREA DE MEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA
653 $aGene silencing
653 $aHemiptera
653 $aPest control
653 $aRNAi
653 $aStink bugs
653 $aSustainable Development Goals (SDGs)
653 $aZero hunger - Goal 2
700 1 $aMURCHIO, S.
700 1 $aCASTRO, A.
700 1 $aCIBILS-STEWART, X.
700 1 $aMIRANDA, P.
700 1 $aMUJICA, V.
700 1 $aPARDO, H.
700 1 $aDALLA RIZZA, M.
773 $tPest Management Science, 2025. [Early view]. https://doi.org/10.1002/ps.8744
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