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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/01/2019 |
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Actualizado : |
24/01/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; BRANDA, A.; ARTIGAS, R.; DUTRA, F.; LLAMBÍ, S. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODY ARTIGAS, Universidad de la República (UdelaR)/ Facultad de Veterinaria; FERNANDO DUTRA, MGAP/ DILAVE (División de Laboratorios de Veterinarios); SILVIA LLAMBÍ, Universidad de la República (UdelaR)/ Facultad de Veterinaria. |
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Título : |
High- resolution melting (HRM) curve analysis: New approach used to detect blad and dumps in Uruguayan Holstein breed. [Análise da curva de alta resolucao (HRM): Nova abordagem usada para detectar blad e dumps em bovinos da raça Holandesa Uruguaia]. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Archives of Veterinary Science, 2018, volume 23, Issue 4, pages 1-9. OPEN ACCESS. |
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DOI : |
10.5380/avs.v23i4.62578 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 29 October 2018 // Accepted 29 November 2018.
This research was supported by the Unit of Biotechnology of INIA Las Brujas. We thank Dr. Marco Dalla Rizza, coordinator of the Unit of Biotechnology, as well as the staff of DNA Bank, located at the same Institute. |
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Contenido : |
ABSTRACT.
The widespread use of artificial insemination has allowed the expansion of genetic progress. However, it also brought consequences such as the expansion of lethal hereditary diseases and the increase in inbreeding. The object of this study was to establish a fast and sensitive molecular assay to detect bovine leukocyte adhesion deficiency (BLAD) and deficiency of uridine monophosphate synthase (DUMPS) carriers in Uruguayan Holstein cattle by means of high resolution melting (HRM) curve analysis. By testing previously confirmed carrier and non-carrier animals, we set up a rapid, simple, and inexpensive diagnostic test using PCR followed by HRM curve analysis. The PCR-HRM genotyping method was effective for the discrimination of BLAD and DUMPS homozygous genotypes, and the BLAD heterozygous genotype. We conclude that the PCR-HRM assay is a robust, reliable, and economical tool for the detection of these mutations in the Holstein breed, which may be implemented in genetic selection programs.
© 2018, Universidade Federal do Parana.
RESUMO.
O uso generalizado de inseminação artificial, permitiu expandir o progresso genético, no entanto, também trouxe consequências na expansão de doenças hereditárias letais e aumento da endogamia. O objetivo deste estudo foi estabelecer um ensaio molecular rápido e sensível para detectar animais portadores da deficiência de adesão leucocitária bovina (BLAD) e da deficiência da uridina monofosfato sintetase (DUMPS) usando a análise de curva de alta resolução (HRM) em bovinos da raça Holandesa Uruguaia. Através do uso de animais portadores e não portadores previamente confirmados, montamos um teste de diagnóstico rápido, simples e de baixo custo usando PCR seguido de análise de curva HRM. O método de genotipagem da PCR-HRM foi efetivo para a discriminação dos genótipos homozigotos BLAD e DUMPS e do genótipo heterozigoto BLAD. Concluímos que o ensaio PCR-HRM é uma ferramenta poderosa, confiável e econômica para a detecção destas mutações na raça Holandesa, o que poderia ser implementado em programas de seleção genética.
© 2018, Universidade Federal do Parana. MenosABSTRACT.
The widespread use of artificial insemination has allowed the expansion of genetic progress. However, it also brought consequences such as the expansion of lethal hereditary diseases and the increase in inbreeding. The object of this study was to establish a fast and sensitive molecular assay to detect bovine leukocyte adhesion deficiency (BLAD) and deficiency of uridine monophosphate synthase (DUMPS) carriers in Uruguayan Holstein cattle by means of high resolution melting (HRM) curve analysis. By testing previously confirmed carrier and non-carrier animals, we set up a rapid, simple, and inexpensive diagnostic test using PCR followed by HRM curve analysis. The PCR-HRM genotyping method was effective for the discrimination of BLAD and DUMPS homozygous genotypes, and the BLAD heterozygous genotype. We conclude that the PCR-HRM assay is a robust, reliable, and economical tool for the detection of these mutations in the Holstein breed, which may be implemented in genetic selection programs.
© 2018, Universidade Federal do Parana.
RESUMO.
O uso generalizado de inseminação artificial, permitiu expandir o progresso genético, no entanto, também trouxe consequências na expansão de doenças hereditárias letais e aumento da endogamia. O objetivo deste estudo foi estabelecer um ensaio molecular rápido e sensível para detectar animais portadores da deficiência de adesão leucocitária bovina (BLAD) e da deficiência da uridina monofosfato sintetase (DUMPS) usando a análise de c... Presentar Todo |
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Palabras claves : |
BLAD; DUMPS; HEIFER; HIGH-RESOLUTION DISSOCIATION CURVE; HOLSTEIN. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03353naa a2200253 a 4500
001 1059450
005 2019-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.5380/avs.v23i4.62578$2DOI
100 1 $aFEDERICI, M.
245 $aHigh- resolution melting (HRM) curve analysis$bNew approach used to detect blad and dumps in Uruguayan Holstein breed. [Análise da curva de alta resolucao (HRM): Nova abordagem usada para detectar blad e dumps em bovinos da raça Holandesa Uruguaia].$h[electronic resource]
260 $c2018
500 $aArticle history: Received 29 October 2018 // Accepted 29 November 2018. This research was supported by the Unit of Biotechnology of INIA Las Brujas. We thank Dr. Marco Dalla Rizza, coordinator of the Unit of Biotechnology, as well as the staff of DNA Bank, located at the same Institute.
520 $aABSTRACT. The widespread use of artificial insemination has allowed the expansion of genetic progress. However, it also brought consequences such as the expansion of lethal hereditary diseases and the increase in inbreeding. The object of this study was to establish a fast and sensitive molecular assay to detect bovine leukocyte adhesion deficiency (BLAD) and deficiency of uridine monophosphate synthase (DUMPS) carriers in Uruguayan Holstein cattle by means of high resolution melting (HRM) curve analysis. By testing previously confirmed carrier and non-carrier animals, we set up a rapid, simple, and inexpensive diagnostic test using PCR followed by HRM curve analysis. The PCR-HRM genotyping method was effective for the discrimination of BLAD and DUMPS homozygous genotypes, and the BLAD heterozygous genotype. We conclude that the PCR-HRM assay is a robust, reliable, and economical tool for the detection of these mutations in the Holstein breed, which may be implemented in genetic selection programs. © 2018, Universidade Federal do Parana. RESUMO. O uso generalizado de inseminação artificial, permitiu expandir o progresso genético, no entanto, também trouxe consequências na expansão de doenças hereditárias letais e aumento da endogamia. O objetivo deste estudo foi estabelecer um ensaio molecular rápido e sensível para detectar animais portadores da deficiência de adesão leucocitária bovina (BLAD) e da deficiência da uridina monofosfato sintetase (DUMPS) usando a análise de curva de alta resolução (HRM) em bovinos da raça Holandesa Uruguaia. Através do uso de animais portadores e não portadores previamente confirmados, montamos um teste de diagnóstico rápido, simples e de baixo custo usando PCR seguido de análise de curva HRM. O método de genotipagem da PCR-HRM foi efetivo para a discriminação dos genótipos homozigotos BLAD e DUMPS e do genótipo heterozigoto BLAD. Concluímos que o ensaio PCR-HRM é uma ferramenta poderosa, confiável e econômica para a detecção destas mutações na raça Holandesa, o que poderia ser implementado em programas de seleção genética. © 2018, Universidade Federal do Parana.
653 $aBLAD
653 $aDUMPS
653 $aHEIFER
653 $aHIGH-RESOLUTION DISSOCIATION CURVE
653 $aHOLSTEIN
700 1 $aBRANDA, A.
700 1 $aARTIGAS, R.
700 1 $aDUTRA, F.
700 1 $aLLAMBÍ, S.
773 $tArchives of Veterinary Science, 2018, volume 23, Issue 4, pages 1-9. OPEN ACCESS.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
30/05/2019 |
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Actualizado : |
04/05/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C. |
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Afiliación : |
FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay. |
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Título : |
Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry.
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837 |
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DOI : |
10.1002/pca.2837 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. |
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Contenido : |
Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. MenosAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methyla... Presentar Todo |
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Palabras claves : |
IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS. |
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Asunto categoría : |
K11 Ingeniería forestal |
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Marc : |
LEADER 02910naa a2200241 a 4500
001 1059792
005 2020-05-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1002/pca.2837$2DOI
100 1 $aWALLACE, F.
245 $aStructural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. .$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00); the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química.
520 $aAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work.
653 $aIMMUNOADJUVANT SAPONINS
653 $aMASS SPECTROMETRY
653 $aMETHYLATION ANALYSIS
653 $aMONOSACCHARIDE ANALYIS
653 $aQUILLAJA BRASILIENSIS
700 1 $aBENNADJI, Z.
700 1 $aFERREIRA, F.
700 1 $aOLIVARO, C.
773 $tPhytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837
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