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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
10/05/2019 |
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Actualizado : |
10/05/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GONZÁLEZ-ARCOS, M.; DE NORONHA FONSECA, M.E.; ZANDONADI, D.B.; PERES, L.E.P.; ARRUABARRENA, A.; FERREIRA, D.S.; KEVEI, Z.; MOHAREB, F.; THOMPSON, A.J.; BOITEUX, L.S. |
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Afiliación : |
MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA ESTHER DE NORONHA FONSECA, Nacional Center for Vegetable Crops Research (CNPH) – Embrapa Vegetable Crops (Hortaliças), Brazil; DANIEL BASÍLIO ZANDONADI, Universidade Federal do Rio de Janeiro (UFRJ), Nupem, Macaé, Brazil; LÁZARO E. P. PERES, Laboratory of Hormonal Control of Plant Development, Departamento de Ciências Biológicas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (ESALQ/USP), Piracicaba, Brazil; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DEMETRYUS S. FERREIRA, Cranfield Soil and Agrifood Institute, Cranfield University, Cranfield, UK.; ZOLTAN KEVEI, Cranfield Soil and Agrifood Institute, Cranfield University, Cranfield, UK.; FADY MOHAREB, Cranfield Soil and Agrifood Institute, Cranfield University, Cranfield, UK.; ANDREW J. THOMPSON, Cranfield Soil and Agrifood Institute, Cranfield University, Cranfield, UK.; LEONARDO S. BOITEUX, Nacional Center for Vegetable Crops Research (CNPH) – Embrapa Vegetable Crops (Hortaliças), Brasília, Brazil. |
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Título : |
A loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Euphytica, 1 May 2019, Volume 215, Issue 5, Article number 95. |
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ISSN : |
0014-2336 |
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DOI : |
10.1007/s10681-019-2418-1 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 21 December 2018 / Accepted: 10 April 2019 / First Online: 16 April 2019.
This work was done in the context of MG-A doctoral studies program at the Facultad de Agronomía, Universidad de la República Oriental del Uruguay. We thank A. Manzzioni, I. Laxague and N. Zunini of INIA Salto Grande, and W. P. Dutra and A. F. Costa of Embrapa Vegetable Crops, for their assistance in conducting some of the experiments. LSB and MENF were supported by CNPq and CAPES grants. AJT and FM were supported by BBSRC Research Grant BB/ L011611/1. |
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Contenido : |
ABSTRACT.
The genetic basis of an erectoid leaf phenotype was investigated in distinct tomato breeding populations, including one derived from Solanum lycopersicum ?LT05? (with the erectoid leaf phenotype and uniform ripening, genotype uu) × S. pimpinellifollium ?TO-937? (with the wild-type leaf phenotype and green fruit shoulder, genotype UU). The erectoid leaf phenotype was inherited as a semi-dominant trait and it co-segregated with the u allele of gene SlGLK2 (Solyc10g008160). This genomic location coincides with a previously described semi-dominant mutation named as Erectoid leaf (Erl). The genomes of ?LT05?, ?TO-937?, and three other unrelated accessions (with the wild-type Erl + allele) were resequenced with the aim of identifying candidate genes. Comparative genomic analyses, including the reference genome ?Heinz 1706? (Erl + allele), identified an Erectoid leaf-specific single nucleotide polymorphism (SNP) in the gene Solyc10g009320. This SNP caused a change of a glutamine codon (present in all the wild-type genomes) to a TAA (= ochre stop-codon) in the Erl allele, resulting in a smaller version of the predicted mutant protein (221 vs. 279 amino acids). Solyc10g009320, previously annotated as an ?unknown protein?, was identified as a TILLER ANGLE CONTROL1-like gene. Linkage between the Erl and Solyc10g009320 was confirmed via Sanger sequencing of the PCR amplicons of the two variant alleles. No recombinants were detected in 265 F 2 individuals. Contrasting S 7 near-isogenic lines were also homozygous for each of the alternate alleles, reinforcing Solyc10g009320 as a strong Erl candidate gene and opening the possibility for fine-tuning manipulation of tomato architecture in breeding programs.
© 2019, Springer Nature B.V. MenosABSTRACT.
The genetic basis of an erectoid leaf phenotype was investigated in distinct tomato breeding populations, including one derived from Solanum lycopersicum ?LT05? (with the erectoid leaf phenotype and uniform ripening, genotype uu) × S. pimpinellifollium ?TO-937? (with the wild-type leaf phenotype and green fruit shoulder, genotype UU). The erectoid leaf phenotype was inherited as a semi-dominant trait and it co-segregated with the u allele of gene SlGLK2 (Solyc10g008160). This genomic location coincides with a previously described semi-dominant mutation named as Erectoid leaf (Erl). The genomes of ?LT05?, ?TO-937?, and three other unrelated accessions (with the wild-type Erl + allele) were resequenced with the aim of identifying candidate genes. Comparative genomic analyses, including the reference genome ?Heinz 1706? (Erl + allele), identified an Erectoid leaf-specific single nucleotide polymorphism (SNP) in the gene Solyc10g009320. This SNP caused a change of a glutamine codon (present in all the wild-type genomes) to a TAA (= ochre stop-codon) in the Erl allele, resulting in a smaller version of the predicted mutant protein (221 vs. 279 amino acids). Solyc10g009320, previously annotated as an ?unknown protein?, was identified as a TILLER ANGLE CONTROL1-like gene. Linkage between the Erl and Solyc10g009320 was confirmed via Sanger sequencing of the PCR amplicons of the two variant alleles. No recombinants were detected in 265 F 2 individuals. Contrasting S 7 near-... Presentar Todo |
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Palabras claves : |
Breeding; Comparative genomic analysis; Plant architecture; Resequencing; Solanum lycopersicum. |
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Thesagro : |
TOMATE. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
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024 7 $a10.1007/s10681-019-2418-1$2DOI
100 1 $aGONZÁLEZ-ARCOS, M.
245 $aA loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 21 December 2018 / Accepted: 10 April 2019 / First Online: 16 April 2019. This work was done in the context of MG-A doctoral studies program at the Facultad de Agronomía, Universidad de la República Oriental del Uruguay. We thank A. Manzzioni, I. Laxague and N. Zunini of INIA Salto Grande, and W. P. Dutra and A. F. Costa of Embrapa Vegetable Crops, for their assistance in conducting some of the experiments. LSB and MENF were supported by CNPq and CAPES grants. AJT and FM were supported by BBSRC Research Grant BB/ L011611/1.
520 $aABSTRACT. The genetic basis of an erectoid leaf phenotype was investigated in distinct tomato breeding populations, including one derived from Solanum lycopersicum ?LT05? (with the erectoid leaf phenotype and uniform ripening, genotype uu) × S. pimpinellifollium ?TO-937? (with the wild-type leaf phenotype and green fruit shoulder, genotype UU). The erectoid leaf phenotype was inherited as a semi-dominant trait and it co-segregated with the u allele of gene SlGLK2 (Solyc10g008160). This genomic location coincides with a previously described semi-dominant mutation named as Erectoid leaf (Erl). The genomes of ?LT05?, ?TO-937?, and three other unrelated accessions (with the wild-type Erl + allele) were resequenced with the aim of identifying candidate genes. Comparative genomic analyses, including the reference genome ?Heinz 1706? (Erl + allele), identified an Erectoid leaf-specific single nucleotide polymorphism (SNP) in the gene Solyc10g009320. This SNP caused a change of a glutamine codon (present in all the wild-type genomes) to a TAA (= ochre stop-codon) in the Erl allele, resulting in a smaller version of the predicted mutant protein (221 vs. 279 amino acids). Solyc10g009320, previously annotated as an ?unknown protein?, was identified as a TILLER ANGLE CONTROL1-like gene. Linkage between the Erl and Solyc10g009320 was confirmed via Sanger sequencing of the PCR amplicons of the two variant alleles. No recombinants were detected in 265 F 2 individuals. Contrasting S 7 near-isogenic lines were also homozygous for each of the alternate alleles, reinforcing Solyc10g009320 as a strong Erl candidate gene and opening the possibility for fine-tuning manipulation of tomato architecture in breeding programs. © 2019, Springer Nature B.V.
650 $aTOMATE
653 $aBreeding
653 $aComparative genomic analysis
653 $aPlant architecture
653 $aResequencing
653 $aSolanum lycopersicum
700 1 $aDE NORONHA FONSECA, M.E.
700 1 $aZANDONADI, D.B.
700 1 $aPERES, L.E.P.
700 1 $aARRUABARRENA, A.
700 1 $aFERREIRA, D.S.
700 1 $aKEVEI, Z.
700 1 $aMOHAREB, F.
700 1 $aTHOMPSON, A.J.
700 1 $aBOITEUX, L.S.
773 $tEuphytica, 1 May 2019, Volume 215, Issue 5, Article number 95.
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| 1. |  | JORNADA ANUAL AREA DE RECUPERACIÓN DE RASTROJOS ARROCEROS, 1977, CIAAB-EEE, TREINTA Y TRES, UY.; VINAY, H.; CHEBATAROFF, N.; ÁLVAREZ, L.; CARDOZO, O.; GRIERSON, J.; MAS, C. Jornada área de recuperación de rastrojos arroceros [Jornada arroz 1977 Estación Experimental del Este, Arrozal 33 S.A.]. Treinta y Tres (Uruguay): CIAAB; EEE, 1977. 48 p.| Biblioteca(s): INIA Treinta y Tres. |
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