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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
01/10/2019 |
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Actualizado : |
01/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BÁEZ, F.; CAMARGO, A.A.; GASTAL, G.D.A. |
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Afiliación : |
FRANCISCO BÁEZ, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; ÁLVARO A. CAMARGO, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Ultrastructural imaging analysis of the zona pellucida surface in bovine oocytes. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Microscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036. |
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DOI : |
10.1017/S1431927619000692 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019. |
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Contenido : |
Abstract:
The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and
mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes
(COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development. |
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Palabras claves : |
BOVINE; IN VITRO MATURATION; PLATAFORMA SALUD ANIMAL; ULTRASTRUCTURE; ZONA PELLUCIDA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02045naa a2200229 a 4500
001 1060255
005 2019-10-01
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1017/S1431927619000692$2DOI
100 1 $aBÁEZ, F.
245 $aUltrastructural imaging analysis of the zona pellucida surface in bovine oocytes.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019.
520 $aAbstract: The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes (COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development.
653 $aBOVINE
653 $aIN VITRO MATURATION
653 $aPLATAFORMA SALUD ANIMAL
653 $aULTRASTRUCTURE
653 $aZONA PELLUCIDA
700 1 $aCAMARGO, A.A.
700 1 $aGASTAL, G.D.A.
773 $tMicroscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036.
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
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Fecha actual : |
13/09/2017 |
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Actualizado : |
18/09/2017 |
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Tipo de producción científica : |
Abstracts/Resúmenes |
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Autor : |
FERRINHO, A.M.; PERIPOLLI, E.; BANCHERO, G.; PEREIRA, A.S.C.; BRITO, G.; LA MANNA, A.; FERNANDEZ, E.; MONTOSSI, F.; BALDI, F. |
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Afiliación : |
School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil; Sao Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences (FCAV), Department of Animal Science, Jaboticabal, Brazil; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidade de São Paulo, Pirassununga, Brazil.; GUSTAVO WALTER BRITO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO LA MANNA ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE GENARO FERNANDEZ RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; School of Agricultural and Veterinarian Sciences, Sao Paulo State University - FCAV/UNESP, Jaboticabal, Brazil. |
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Título : |
Effect of growth rate on beef fatty acid profile from Hereford steers finished either on pasture or in feedlot. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Journal of Animal Science, 2017, v.95, suppl.4. p.180-181. |
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DOI : |
10.2527/asasann.2017.365 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published online: August 10, 2017. |
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Contenido : |
Abstract: Different nutritional management during the rearing and finishing periods can determine changes on beef fatty acid profile.
Beef lipid composition was quantified in 224 male Hereford calves weaned at 8 wk of age with an average initial live
weight (LW) of 170 ± 17 kg. After weaning, 4 nutritional treatments were imposed to obtain different daily LW gains (LWG;
kg/d) during the first winter. Nutritional management groups were high LWG in feedlot (HF), low LWG in feedlot (LF),
high LWG in pasture (HP), and low LWG in pasture (LP). The finishing phase began when each group reached a mean LW of
350 ± 28 kg. During the finishing phase, one-half of the HF, LF, HP and LP animals were finished on pasture and the other
half in a feedlot. The animals were slaughtered when calves in each treatment attained a mean LW of 500 kg. Analysis
of beef fatty acids was performed by extraction and methylation from LM, and the fatty acids were quantified using a gas
chromatography. The statistical model included the groups at growing phase (HF, LF, HP, and LP) and the groups at finishing
phase (feedlot or pasture) and the interaction between growing and finishing phase as fixed effects and LW at the
beginning of the finishing phase as a covariable. Tukey?s test was applied to compare the means (P < 0.05). There was interaction
between the growing and finishing phases for myristic acid and CLA cis 9, trans 11. Animals from HF finished on
pasture presented the highest concentration of myristic acid (2.54%) when compared with the other treatments. The LP
and HP groups finished on pasture had more CLA cis 9, trans 11 in their beef composition (0.60 and 0.58%, respectively),
whereas animals finished in the feedlot presented the lowest concentrations (average of 0.28%). Finishing phase affected
the fatty acid profile (P < 0.05). Higher concentrations of palmitic, oleic, and linoleic acids were observed in beef from
animals finished in the feedlot than in beef from those finished on pasture. However, the beef from animals finished on pasture
presented high concentrations of stearic, docosapentaenoic, and docosaexaenoic acids. Animals finished on pasture
had higher concentrations of linolenic, arachidonic, and eicosapentaenoic acids compared with those finished in feedlot. Interestingly,
the LP group presented higher concentrations of linolenic and eicosapentaenoic acids, regardless of the finishing
phase. The same results were observed in the LF group for arachidonic acid. In general, the LP and HP groups finished on
pasture resulted in healthy beefs, with the greatest CLA and omega-3 concentrations. MenosAbstract: Different nutritional management during the rearing and finishing periods can determine changes on beef fatty acid profile.
Beef lipid composition was quantified in 224 male Hereford calves weaned at 8 wk of age with an average initial live
weight (LW) of 170 ± 17 kg. After weaning, 4 nutritional treatments were imposed to obtain different daily LW gains (LWG;
kg/d) during the first winter. Nutritional management groups were high LWG in feedlot (HF), low LWG in feedlot (LF),
high LWG in pasture (HP), and low LWG in pasture (LP). The finishing phase began when each group reached a mean LW of
350 ± 28 kg. During the finishing phase, one-half of the HF, LF, HP and LP animals were finished on pasture and the other
half in a feedlot. The animals were slaughtered when calves in each treatment attained a mean LW of 500 kg. Analysis
of beef fatty acids was performed by extraction and methylation from LM, and the fatty acids were quantified using a gas
chromatography. The statistical model included the groups at growing phase (HF, LF, HP, and LP) and the groups at finishing
phase (feedlot or pasture) and the interaction between growing and finishing phase as fixed effects and LW at the
beginning of the finishing phase as a covariable. Tukey?s test was applied to compare the means (P < 0.05). There was interaction
between the growing and finishing phases for myristic acid and CLA cis 9, trans 11. Animals from HF finished on
pasture presented the highest concentration of myri... Presentar Todo |
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Palabras claves : |
ENGORDE A CORRAL; FINISHING SYSTEM; GROW-OUT; LIPID COMPOSITION; NOVILLOS HEREFORD; PASTURA. |
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Thesagro : |
CARNE VACUNA; NOVILLOS; URUGUAY. |
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Asunto categoría : |
--
L01 Ganadería |
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Marc : |
LEADER 03652naa a2200349 a 4500
001 1057563
005 2017-09-18
008 2017 bl uuuu u00u1 u #d
024 7 $a10.2527/asasann.2017.365$2DOI
100 1 $aFERRINHO, A.M.
245 $aEffect of growth rate on beef fatty acid profile from Hereford steers finished either on pasture or in feedlot.$h[electronic resource]
260 $c2017
500 $aArticle history: Published online: August 10, 2017.
520 $aAbstract: Different nutritional management during the rearing and finishing periods can determine changes on beef fatty acid profile. Beef lipid composition was quantified in 224 male Hereford calves weaned at 8 wk of age with an average initial live weight (LW) of 170 ± 17 kg. After weaning, 4 nutritional treatments were imposed to obtain different daily LW gains (LWG; kg/d) during the first winter. Nutritional management groups were high LWG in feedlot (HF), low LWG in feedlot (LF), high LWG in pasture (HP), and low LWG in pasture (LP). The finishing phase began when each group reached a mean LW of 350 ± 28 kg. During the finishing phase, one-half of the HF, LF, HP and LP animals were finished on pasture and the other half in a feedlot. The animals were slaughtered when calves in each treatment attained a mean LW of 500 kg. Analysis of beef fatty acids was performed by extraction and methylation from LM, and the fatty acids were quantified using a gas chromatography. The statistical model included the groups at growing phase (HF, LF, HP, and LP) and the groups at finishing phase (feedlot or pasture) and the interaction between growing and finishing phase as fixed effects and LW at the beginning of the finishing phase as a covariable. Tukey?s test was applied to compare the means (P < 0.05). There was interaction between the growing and finishing phases for myristic acid and CLA cis 9, trans 11. Animals from HF finished on pasture presented the highest concentration of myristic acid (2.54%) when compared with the other treatments. The LP and HP groups finished on pasture had more CLA cis 9, trans 11 in their beef composition (0.60 and 0.58%, respectively), whereas animals finished in the feedlot presented the lowest concentrations (average of 0.28%). Finishing phase affected the fatty acid profile (P < 0.05). Higher concentrations of palmitic, oleic, and linoleic acids were observed in beef from animals finished in the feedlot than in beef from those finished on pasture. However, the beef from animals finished on pasture presented high concentrations of stearic, docosapentaenoic, and docosaexaenoic acids. Animals finished on pasture had higher concentrations of linolenic, arachidonic, and eicosapentaenoic acids compared with those finished in feedlot. Interestingly, the LP group presented higher concentrations of linolenic and eicosapentaenoic acids, regardless of the finishing phase. The same results were observed in the LF group for arachidonic acid. In general, the LP and HP groups finished on pasture resulted in healthy beefs, with the greatest CLA and omega-3 concentrations.
650 $aCARNE VACUNA
650 $aNOVILLOS
650 $aURUGUAY
653 $aENGORDE A CORRAL
653 $aFINISHING SYSTEM
653 $aGROW-OUT
653 $aLIPID COMPOSITION
653 $aNOVILLOS HEREFORD
653 $aPASTURA
700 1 $aPERIPOLLI, E.
700 1 $aBANCHERO, G.
700 1 $aPEREIRA, A.S.C.
700 1 $aBRITO, G.
700 1 $aLA MANNA, A.
700 1 $aFERNANDEZ, E.
700 1 $aMONTOSSI, F.
700 1 $aBALDI, F.
773 $tJournal of Animal Science, 2017$gv.95, suppl.4. p.180-181.
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