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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
25/09/2020 |
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Actualizado : |
25/09/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CUNHA, F.; CUSHMAN, R.A.; SANTA CRUZ, R.; DE NAVA, G.; VIÑOLES, C. |
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Afiliación : |
FERNANDO CUNHA, Masters Student, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. Liberal profession vet, Uruguay; ROBERT A. CUSHMAN, USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA; RODRIGO SANTA CRUZ, Polo Agroforestal, Estación Experimental Bernardo Rosengurtt, Facultad de Agronomía, Universidad de la República, Cerro Largo, Uruguay; GUILLERMO DE NAVA, Liberal profession vet, Uruguay; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Polo Agroforestal, Estación Experimental Bernardo Rosengurtt, Facultad de Agronomía, Universidad de la República, Cerro Largo, Uruguay. |
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Título : |
Antral follicular count has limited impact in the selection of more fertile beef heifers. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Livestock Science, November 2020, Volume 241, Article number 104230. Doi: https://doi.org/10.1016/j.livsci.2020.104230 |
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DOI : |
DOI: 10.1016/j.livsci.2020.104230 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 7 April 2020, Revised 20 July 2020, Accepted 25 August 2020, Available online 26 August 2020. Acknowledgments: This work was supported by the project ?Marcadores de Fertilidad para Ganado de Carne? of the National Institute of Agricultural Research (INIA). This research was partially supported by ARS Research Project 3040-31000-096-00D (RAC). The authors would like to acknowledge Pablo Cuadro from INIA and all the staff of the commercial farm ?Las Grutas?. We would also like to thank Pablo González for his
contribution with statistic. Corresponding author: E-mail address: cvinoles@adinet.com.uy (C. Viñoles). |
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Contenido : |
ABSTRACT: The selection of replacements using applications such as reproductive tract scores and antral follicle count may be useful to eliminate less fertile animals before breeding. Previously, we reported that heifers with increased numbers of follicles achieved puberty earlier than heifers with diminished numbers of follicles. We, therefore, hypothesized that heifers that achieved a reproductive tract score ?4 earlier in the breeding season would have greater numbers of follicles than heifers that achieved a reproductive tract score ?4 later in the breeding season. The uterus and ovaries of three hundred and fifty beef heifers from a commercial farm were evaluated by transrectal ultrasonography 20 days before breeding and parameters of growth and development were recorded. The antral follicle count (AFC) of both ovaries was recorded and the heifers assigned a reproductive tract score (1
to 5). Only heifers with a reproductive tract score ?4 entered to two fixed timed artificial insemination (FTAI) programs. The first group (FTAI1 , n = 269) started the day of the beginning of the breeding period and the second group (FTAI2 , n = 61) one month later. The rest were naturally mated (no FTAI, n = 20). Serial pregnancy scanning was performed throughout the breeding season and 45 days after bull removal to estimate the day of conception. Data indicative of growth and development were analysed using the REG and GLM
procedures of SAS with FTAI (0, 1 and 2), AFC (low, medium and high), and the interaction as the independent variables. Reproductive tract development was negatively associated to AFC (P>0.0001). There was no interaction between FTAI and AFC (P>0.11). Heifers inseminated at FTAI1 were heavier and taller, had greater deposition of fat and muscle, and conceived earlier than those inseminated at FTAI2 (P<0.05); however, there was no difference in AFC between heifers inseminated in FTAI1 and FTAI2 (15.6 ± 0.4 vs. 16.7 ± 0.9 follicles, respectively; P = 0.3). Furthermore, conception rates did not differ statistically between heifers in FTAI1 and FTAI2 (P = 0.12). Antral follicle count influenced growth and carcass traits (P>0.05) and heifers in the High AFC class conceived approximately 1 week earlier than heifers in the Low and Medium AFC class (P<0.05). We conclude that pubertal heifers have less AFC, thus similar numbers were observed for heifers in both FTAI programs and no differences in pregnancy rate. Antral follicle count may allow for the selection of heifers that conceive earlier, but may result in a lower pregnancy rate. MenosABSTRACT: The selection of replacements using applications such as reproductive tract scores and antral follicle count may be useful to eliminate less fertile animals before breeding. Previously, we reported that heifers with increased numbers of follicles achieved puberty earlier than heifers with diminished numbers of follicles. We, therefore, hypothesized that heifers that achieved a reproductive tract score ?4 earlier in the breeding season would have greater numbers of follicles than heifers that achieved a reproductive tract score ?4 later in the breeding season. The uterus and ovaries of three hundred and fifty beef heifers from a commercial farm were evaluated by transrectal ultrasonography 20 days before breeding and parameters of growth and development were recorded. The antral follicle count (AFC) of both ovaries was recorded and the heifers assigned a reproductive tract score (1
to 5). Only heifers with a reproductive tract score ?4 entered to two fixed timed artificial insemination (FTAI) programs. The first group (FTAI1 , n = 269) started the day of the beginning of the breeding period and the second group (FTAI2 , n = 61) one month later. The rest were naturally mated (no FTAI, n = 20). Serial pregnancy scanning was performed throughout the breeding season and 45 days after bull removal to estimate the day of conception. Data indicative of growth and development were analysed using the REG and GLM
procedures of SAS with FTAI (0, 1 and 2), AFC (low, medium and ... Presentar Todo |
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Palabras claves : |
ANTRAL FOLLICLE COUNT; BEEF HEIFERS; FIXED TIME ARTIFICIAL INSEMINATION; PUBERTY; REPRODUCCIÓN ANIMAL. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 04044naa a2200253 a 4500
001 1061348
005 2020-09-25
008 2020 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1016/j.livsci.2020.104230$2DOI
100 1 $aCUNHA, F.
245 $aAntral follicular count has limited impact in the selection of more fertile beef heifers.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 7 April 2020, Revised 20 July 2020, Accepted 25 August 2020, Available online 26 August 2020. Acknowledgments: This work was supported by the project ?Marcadores de Fertilidad para Ganado de Carne? of the National Institute of Agricultural Research (INIA). This research was partially supported by ARS Research Project 3040-31000-096-00D (RAC). The authors would like to acknowledge Pablo Cuadro from INIA and all the staff of the commercial farm ?Las Grutas?. We would also like to thank Pablo González for his contribution with statistic. Corresponding author: E-mail address: cvinoles@adinet.com.uy (C. Viñoles).
520 $aABSTRACT: The selection of replacements using applications such as reproductive tract scores and antral follicle count may be useful to eliminate less fertile animals before breeding. Previously, we reported that heifers with increased numbers of follicles achieved puberty earlier than heifers with diminished numbers of follicles. We, therefore, hypothesized that heifers that achieved a reproductive tract score ?4 earlier in the breeding season would have greater numbers of follicles than heifers that achieved a reproductive tract score ?4 later in the breeding season. The uterus and ovaries of three hundred and fifty beef heifers from a commercial farm were evaluated by transrectal ultrasonography 20 days before breeding and parameters of growth and development were recorded. The antral follicle count (AFC) of both ovaries was recorded and the heifers assigned a reproductive tract score (1 to 5). Only heifers with a reproductive tract score ?4 entered to two fixed timed artificial insemination (FTAI) programs. The first group (FTAI1 , n = 269) started the day of the beginning of the breeding period and the second group (FTAI2 , n = 61) one month later. The rest were naturally mated (no FTAI, n = 20). Serial pregnancy scanning was performed throughout the breeding season and 45 days after bull removal to estimate the day of conception. Data indicative of growth and development were analysed using the REG and GLM procedures of SAS with FTAI (0, 1 and 2), AFC (low, medium and high), and the interaction as the independent variables. Reproductive tract development was negatively associated to AFC (P>0.0001). There was no interaction between FTAI and AFC (P>0.11). Heifers inseminated at FTAI1 were heavier and taller, had greater deposition of fat and muscle, and conceived earlier than those inseminated at FTAI2 (P<0.05); however, there was no difference in AFC between heifers inseminated in FTAI1 and FTAI2 (15.6 ± 0.4 vs. 16.7 ± 0.9 follicles, respectively; P = 0.3). Furthermore, conception rates did not differ statistically between heifers in FTAI1 and FTAI2 (P = 0.12). Antral follicle count influenced growth and carcass traits (P>0.05) and heifers in the High AFC class conceived approximately 1 week earlier than heifers in the Low and Medium AFC class (P<0.05). We conclude that pubertal heifers have less AFC, thus similar numbers were observed for heifers in both FTAI programs and no differences in pregnancy rate. Antral follicle count may allow for the selection of heifers that conceive earlier, but may result in a lower pregnancy rate.
653 $aANTRAL FOLLICLE COUNT
653 $aBEEF HEIFERS
653 $aFIXED TIME ARTIFICIAL INSEMINATION
653 $aPUBERTY
653 $aREPRODUCCIÓN ANIMAL
700 1 $aCUSHMAN, R.A.
700 1 $aSANTA CRUZ, R.
700 1 $aDE NAVA, G.
700 1 $aVIÑOLES, C.
773 $tLivestock Science, November 2020, Volume 241, Article number 104230. Doi: https://doi.org/10.1016/j.livsci.2020.104230
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
30/05/2019 |
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Actualizado : |
04/05/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C. |
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Afiliación : |
FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay. |
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Título : |
Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry.
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837 |
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DOI : |
10.1002/pca.2837 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. |
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Contenido : |
Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. MenosAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methyla... Presentar Todo |
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Palabras claves : |
IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS. |
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Asunto categoría : |
K11 Ingeniería forestal |
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Marc : |
LEADER 02910naa a2200241 a 4500
001 1059792
005 2020-05-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1002/pca.2837$2DOI
100 1 $aWALLACE, F.
245 $aStructural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. .$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00); the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química.
520 $aAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work.
653 $aIMMUNOADJUVANT SAPONINS
653 $aMASS SPECTROMETRY
653 $aMETHYLATION ANALYSIS
653 $aMONOSACCHARIDE ANALYIS
653 $aQUILLAJA BRASILIENSIS
700 1 $aBENNADJI, Z.
700 1 $aFERREIRA, F.
700 1 $aOLIVARO, C.
773 $tPhytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837
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