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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
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Fecha : |
01/12/2020 |
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Actualizado : |
21/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PARODI, P.; CORBELLINI, L.G.; LEOTTI, V.B.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M.; ARMÚA-FERNÁNDEZ, M.T. |
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Afiliación : |
PABLO ANDRÉS PARODI TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS G. CORBELLINI, Laboratório de Epidemiología Veterinária, Facultad de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; VANESSA B. LEOTTI, Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; RODOLFO RIVERO, Laboratorio Regional Noroeste “Miguel C. Rubino”, División de Laboratorios Veterinarios “Miguel C. Rubino”, Paysandú, Uruguay; MÓNICA CECILIA MIRABALLES FERRER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ M. VENZAL, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay. |
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Título : |
Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report) |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742 |
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DOI : |
10.1177/1040638720975742 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com |
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Contenido : |
Abstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent. |
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Palabras claves : |
ANAPLASMA MARGINALE; BABESIA SPP; CATTLE; ENFERMEDADES DE LOS ANIMALES; MULTIPLEX PCR; PLATAFORMA DE SALUD ANIMAL; SENSITIVITY; SPECIFICITY; TICK-BORNE DISEASES. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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Marc : |
LEADER 02587naa a2200337 a 4500
001 1061695
005 2021-05-21
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1177/1040638720975742$2DOI
100 1 $aPARODI, P.
245 $aValidation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report)$h[electronic resource]
260 $c2021
500 $aArticle history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com
520 $aAbstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent.
653 $aANAPLASMA MARGINALE
653 $aBABESIA SPP
653 $aCATTLE
653 $aENFERMEDADES DE LOS ANIMALES
653 $aMULTIPLEX PCR
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSENSITIVITY
653 $aSPECIFICITY
653 $aTICK-BORNE DISEASES
700 1 $aCORBELLINI, L.G.
700 1 $aLEOTTI, V.B.
700 1 $aRIVERO, R.
700 1 $aMIRABALLES, C.
700 1 $aRIET-CORREA, F.
700 1 $aVENZAL, J.M.
700 1 $aARMÚA-FERNÁNDEZ, M.T.
773 $tJournal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
21/12/2020 |
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Actualizado : |
21/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FERRINHO, A.M.; PERIPOLLI, E.; BANCHERO, G.; PEREIRA, A.S.C.; BRITO, G.; LA MANNA, A.; FERNANDEZ, E.; MONTOSSI, F.; KLUSKA, S.; MUELLER, L.F.; BERCHIELLI, T.T.; BALDI, F. |
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Afiliación : |
ADRIELLE MATIAS FERRINHO, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil; Instituto Nacional de Ciência e Tecnologia-Ciencia Animal, Viçosa, MG, Brazil; ELISA PERIPOLLI, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANGÉLICA SIMONE CRAVO PEREIRA, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil; GUSTAVO WALTER BRITO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO LA MANNA ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE GENARO FERNANDEZ RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SABRINA KLUSKA, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil; LENISE FREITAS MUELLER, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil; TELMA TERESINHA BERCHIELLI, Dpto Zootecnia, Fac. Ciências Agrárias e Veterinárias, Univ. Estadual Paulista, SP, Brazil; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brasília, DF, Brazil; Instituto Nacional de Ciência e Tecnologia-Ciencia Animal, Viçosa, MG, Brazil; FERNANDO SEBASTIAN BALDI REY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Effect of growth path on carcass and meat-quality traits of Hereford steers finished on pasture or in feedlot. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Animal Production Science, 2020, Volume 60, Issue 2, Pages 323-332. Doi: https://doi.org/10.1071/AN18075 |
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ISSN : |
1836-0939 |
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DOI : |
10.1071/AN18075 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 January 2018 / Accepted 14 May 2019 / Published online 4 December 2019.
Funding information:
This work was supported by Sao Paulo Research Foundation (FAPESP grant #2016/22022-4 and #2011/21241-0), Brazil, and the National Institute of Agricultural research (INIA) of Uruguay. |
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Contenido : |
ABSTRACT.
The objective of this study was to evaluate the effect of nutritional management during rearing and finishing phases on beef fatty acid composition, and carcass and beef quality traits of Hereford cattle. The study used 240 castrated male calves weaned at 8 months of age, and with an average weight of 170 ± 17 kg. After weaning, the experiment was divided in to three phases in a 4 × 2 factorial design: a 93-day winter period with four treatment groups (on pasture or in feedlot and at high or low feeding levels); a 196-day compensatory-growth phase on pasture; and a finishing phase either on pasture or in feedlot. Animals were slaughtered when each group attained a mean liveweight of 500 kg. The winter growth × finishing management interaction significantly affected hot carcass weight (P = 0.0029). There was no differences observed for feedlot-finished steers, but for pasture-finished steers, those pasture-reared had higher hot carcass weight (kg) than those feedlot-reared (low pasture 256.30 ± 1.60, high pasture 253.72 ± 1.60, low feedlot 249.85 ± 1.66, high feedlot 247.60 ± 1.62). Feedlot-finished steers showed higher (P < 0.05) mean values than pasture-finished steers for ribeye area (55.61 ± 0.69 cm2 vs 53.18 cm2), backfat thickness (8.62 ± 0.32 mm vs 6.21 mm), marbling score (237.97 ± 13.06 vs 171.70) and final pH (5.53 ± 0.02 vs 5.48). Additionally, feedlot-finished steers raised in feedlot during the winter-growth period displayed the heaviest hindquarter cuts. Meat from pasture-finished steers had lower (P < 0.05) shear-force values than from feedlot-finished cattle (2.95 ± 0.18 vs 3.66 ± 0.17 kg), and when reared on either high or low pasture during winter-growth, they showed the highest (P < 0.05) conjugated linoleic acid (cis-9, trans-11) and n-3 polyunsaturated fatty acid concentrations. In conclusion, growing and finishing cattle on pasture improved the carcass yield of retail cuts because of low fat concentration, and improved the nutritional and health value of the beef fatty acid profile.
© 2020 CSIRO. MenosABSTRACT.
The objective of this study was to evaluate the effect of nutritional management during rearing and finishing phases on beef fatty acid composition, and carcass and beef quality traits of Hereford cattle. The study used 240 castrated male calves weaned at 8 months of age, and with an average weight of 170 ± 17 kg. After weaning, the experiment was divided in to three phases in a 4 × 2 factorial design: a 93-day winter period with four treatment groups (on pasture or in feedlot and at high or low feeding levels); a 196-day compensatory-growth phase on pasture; and a finishing phase either on pasture or in feedlot. Animals were slaughtered when each group attained a mean liveweight of 500 kg. The winter growth × finishing management interaction significantly affected hot carcass weight (P = 0.0029). There was no differences observed for feedlot-finished steers, but for pasture-finished steers, those pasture-reared had higher hot carcass weight (kg) than those feedlot-reared (low pasture 256.30 ± 1.60, high pasture 253.72 ± 1.60, low feedlot 249.85 ± 1.66, high feedlot 247.60 ± 1.62). Feedlot-finished steers showed higher (P < 0.05) mean values than pasture-finished steers for ribeye area (55.61 ± 0.69 cm2 vs 53.18 cm2), backfat thickness (8.62 ± 0.32 mm vs 6.21 mm), marbling score (237.97 ± 13.06 vs 171.70) and final pH (5.53 ± 0.02 vs 5.48). Additionally, feedlot-finished steers raised in feedlot during the winter-growth period displayed the heaviest hindquarter cut... Presentar Todo |
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Palabras claves : |
Carry-over effects; Fatty-acid profile; Finishing system; Growth-out. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 03376naa a2200337 a 4500
001 1060696
005 2020-12-21
008 2020 bl uuuu u00u1 u #d
022 $a1836-0939
024 7 $a10.1071/AN18075$2DOI
100 1 $aFERRINHO, A.M.
245 $aEffect of growth path on carcass and meat-quality traits of Hereford steers finished on pasture or in feedlot.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 30 January 2018 / Accepted 14 May 2019 / Published online 4 December 2019. Funding information: This work was supported by Sao Paulo Research Foundation (FAPESP grant #2016/22022-4 and #2011/21241-0), Brazil, and the National Institute of Agricultural research (INIA) of Uruguay.
520 $aABSTRACT. The objective of this study was to evaluate the effect of nutritional management during rearing and finishing phases on beef fatty acid composition, and carcass and beef quality traits of Hereford cattle. The study used 240 castrated male calves weaned at 8 months of age, and with an average weight of 170 ± 17 kg. After weaning, the experiment was divided in to three phases in a 4 × 2 factorial design: a 93-day winter period with four treatment groups (on pasture or in feedlot and at high or low feeding levels); a 196-day compensatory-growth phase on pasture; and a finishing phase either on pasture or in feedlot. Animals were slaughtered when each group attained a mean liveweight of 500 kg. The winter growth × finishing management interaction significantly affected hot carcass weight (P = 0.0029). There was no differences observed for feedlot-finished steers, but for pasture-finished steers, those pasture-reared had higher hot carcass weight (kg) than those feedlot-reared (low pasture 256.30 ± 1.60, high pasture 253.72 ± 1.60, low feedlot 249.85 ± 1.66, high feedlot 247.60 ± 1.62). Feedlot-finished steers showed higher (P < 0.05) mean values than pasture-finished steers for ribeye area (55.61 ± 0.69 cm2 vs 53.18 cm2), backfat thickness (8.62 ± 0.32 mm vs 6.21 mm), marbling score (237.97 ± 13.06 vs 171.70) and final pH (5.53 ± 0.02 vs 5.48). Additionally, feedlot-finished steers raised in feedlot during the winter-growth period displayed the heaviest hindquarter cuts. Meat from pasture-finished steers had lower (P < 0.05) shear-force values than from feedlot-finished cattle (2.95 ± 0.18 vs 3.66 ± 0.17 kg), and when reared on either high or low pasture during winter-growth, they showed the highest (P < 0.05) conjugated linoleic acid (cis-9, trans-11) and n-3 polyunsaturated fatty acid concentrations. In conclusion, growing and finishing cattle on pasture improved the carcass yield of retail cuts because of low fat concentration, and improved the nutritional and health value of the beef fatty acid profile. © 2020 CSIRO.
653 $aCarry-over effects
653 $aFatty-acid profile
653 $aFinishing system
653 $aGrowth-out
700 1 $aPERIPOLLI, E.
700 1 $aBANCHERO, G.
700 1 $aPEREIRA, A.S.C.
700 1 $aBRITO, G.
700 1 $aLA MANNA, A.
700 1 $aFERNANDEZ, E.
700 1 $aMONTOSSI, F.
700 1 $aKLUSKA, S.
700 1 $aMUELLER, L.F.
700 1 $aBERCHIELLI, T.T.
700 1 $aBALDI, F.
773 $tAnimal Production Science, 2020, Volume 60, Issue 2, Pages 323-332. Doi: https://doi.org/10.1071/AN18075
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