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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
22/02/2021 |
Actualizado : |
22/02/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
LEONI, C.; DUARTE, F.; SPERONI, G.; SILVERA, M.; IRIARTE, W.; BONNECARRERE, V. |
Afiliación : |
CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELICIA DUARTE BAREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; G. SPERONI, Departamento de Biolog??a Vegetal, Facultad de Agronom??a, Universidad de la Repu?blica, Montevideo, Uruguay.; MARIANA SILVERA ORREGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WANDA IRIARTE GRECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Etiology of pear flower bud necrosis in Uruguay. [Conference paper]. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Acta Horticulturae, February 2021, N°1303, p. 359-366. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.50 |
ISSN : |
0567-7572 (print); 2406-6168 (electronic) |
DOI : |
10.17660/ActaHortic.2021.1303.50 |
Idioma : |
Inglés |
Notas : |
Article history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. |
Contenido : |
Abstract:
Pear flower bud necrosis (PFBN) has been observed in Uruguayan orchards affecting the productivity of the trees. Severity of the problem is highly variable across years and despite the fact that it is observed in several countries, the etiology of the disease is not always clear. To elucidate the cause of PFBN, different activities were implemented over several years: surveys of pear orchards to quantify PFBN severity, morphological and histological studies of diseased buds, and isolation of potential pathogenic microorganisms from affected tissues. From the orchard surveys no clear association between climatic conditions (mean temperatures, precipitation, chilling hours) and PFBN were detected for the five years evaluated. Flower buds collected one month before flowering and fixed in formalin-ethanol-acetic acid solution showed necrosis associated with tumour development in bud primordia, and histological preparations from those tumours showed cell hypertrophy and hyperplasia. Finally, from the diseased buds we consistently isolated bacteria and no fungi. The bacteria were gram negative, oxidase positive, fluorescent on King B medium, produced indole-3-acetic acid and were positive in the tobacco hypersensitivity test. We sequenced the 16S RNA genes of the isolated bacteria to perform a taxonomic classification. We conclude that PFBN in Uruguay is caused by bacteria belonging to the Pseudomonas genus.
@ International Society for Horticultural Science. |
Palabras claves : |
WILLIAM'S. |
Thesagro : |
PSEUDOMONAS SP; PYRUS COMMUNIS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02505naa a2200253 a 4500 001 1061746 005 2021-02-22 008 2021 bl uuuu u00u1 u #d 022 $a0567-7572 (print); 2406-6168 (electronic) 024 7 $a10.17660/ActaHortic.2021.1303.50$2DOI 100 1 $aLEONI, C. 245 $aEtiology of pear flower bud necrosis in Uruguay. [Conference paper].$h[electronic resource] 260 $c2021 500 $aArticle history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. 520 $aAbstract: Pear flower bud necrosis (PFBN) has been observed in Uruguayan orchards affecting the productivity of the trees. Severity of the problem is highly variable across years and despite the fact that it is observed in several countries, the etiology of the disease is not always clear. To elucidate the cause of PFBN, different activities were implemented over several years: surveys of pear orchards to quantify PFBN severity, morphological and histological studies of diseased buds, and isolation of potential pathogenic microorganisms from affected tissues. From the orchard surveys no clear association between climatic conditions (mean temperatures, precipitation, chilling hours) and PFBN were detected for the five years evaluated. Flower buds collected one month before flowering and fixed in formalin-ethanol-acetic acid solution showed necrosis associated with tumour development in bud primordia, and histological preparations from those tumours showed cell hypertrophy and hyperplasia. Finally, from the diseased buds we consistently isolated bacteria and no fungi. The bacteria were gram negative, oxidase positive, fluorescent on King B medium, produced indole-3-acetic acid and were positive in the tobacco hypersensitivity test. We sequenced the 16S RNA genes of the isolated bacteria to perform a taxonomic classification. We conclude that PFBN in Uruguay is caused by bacteria belonging to the Pseudomonas genus. @ International Society for Horticultural Science. 650 $aPSEUDOMONAS SP 650 $aPYRUS COMMUNIS 653 $aWILLIAM'S 700 1 $aDUARTE, F. 700 1 $aSPERONI, G. 700 1 $aSILVERA, M. 700 1 $aIRIARTE, W. 700 1 $aBONNECARRERE, V. 773 $tActa Horticulturae, February 2021, N°1303, p. 359-366. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.50
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
25/06/2018 |
Actualizado : |
22/07/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
PEREIRA, J.; BERNAL, J.; MARTINELLI, L.; VILLAMIL, J.J.; CONDE, P. |
Afiliación : |
J. PEREIRA, Universidad de la República (UdelaR)/ Facultad de Agronomía; J. BERNAL, Universidad de la República (UdelaR)/ Facultad de Agronomía; L. MARTINELLI, Universidad de la República (UdelaR)/ Facultad de Agronomía; JUAN JOSE VILLAMIL SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA PAULA CONDE INNAMORATO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Original olive genotypes found in Uruguay identified by morphological and molecular markers. |
Complemento del título : |
Conference Paper. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Acta Horticulturae, May 2018, N° 1199, p. 7-13. |
Serie : |
(Acta Horticulturae; 1199) |
ISBN : |
978-94-62611-95-5 |
ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
DOI : |
10.17660/ActaHortic.2018.1199.2 |
Idioma : |
Inglés |
Notas : |
In: Acta Horticulturae (ISHS) 1199: VIII International Olive Symposium. Editors: S. Perica, G. Vuletin Selak, T. Klepo, L. Ferguson, L. Sebastiani. |
Contenido : |
Abstract
Montevideo, the capital city of Uruguay, founded by Spaniards in the 18th century, and Colonia del Sacramento, established by the Portuguese in the 17th century, are sources of olive diversity. A study and analysis of this unexplored heritage was initiated 10 years ago, using morphological and molecular tools. After a primary geographical and historical survey, 360 Olea europaea fruit and leaf samples were collected. After morphological analysis of 11 stone characters, the samples could not be matched with any cultivars in databases from Mediterranean countries. When a set of 14 pairs of microsatellite primers was applied, nine different multilocus genotypes were detected that were not recorded in the databases. In agreement with the colonization process and olive tree introduction routes into South America, two groups of genotypes were distinguished: a) seven ?genetic mosaic? genotypes related to genotypes from Spain and Portugal, introduced to Uruguay in areas under Spanish control, and b) two genotypes with one of two alleles in less than 70% with molecular variants found in Hispanic and Portuguese cultivars. Because of their height and trunk size, these trees were assumed to be older. Based on this information, we conclude that Uruguay has preserved a unique and original gene pool, currently productive and adapted to local soil and climatic conditions. This pool is a heritage of global interest, suitable for commercial and cultural purposes.
@ International Society for Horticultural Science. MenosAbstract
Montevideo, the capital city of Uruguay, founded by Spaniards in the 18th century, and Colonia del Sacramento, established by the Portuguese in the 17th century, are sources of olive diversity. A study and analysis of this unexplored heritage was initiated 10 years ago, using morphological and molecular tools. After a primary geographical and historical survey, 360 Olea europaea fruit and leaf samples were collected. After morphological analysis of 11 stone characters, the samples could not be matched with any cultivars in databases from Mediterranean countries. When a set of 14 pairs of microsatellite primers was applied, nine different multilocus genotypes were detected that were not recorded in the databases. In agreement with the colonization process and olive tree introduction routes into South America, two groups of genotypes were distinguished: a) seven ?genetic mosaic? genotypes related to genotypes from Spain and Portugal, introduced to Uruguay in areas under Spanish control, and b) two genotypes with one of two alleles in less than 70% with molecular variants found in Hispanic and Portuguese cultivars. Because of their height and trunk size, these trees were assumed to be older. Based on this information, we conclude that Uruguay has preserved a unique and original gene pool, currently productive and adapted to local soil and climatic conditions. This pool is a heritage of global interest, suitable for commercial and cultural purposes.
@ International Soci... Presentar Todo |
Palabras claves : |
ANCIENT OLIVE TREES; EVOLUTION; MOLECULAR; MORPHOLOGICAL MARKERS. |
Thesagro : |
OLIVOS; URUGUAY. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02555naa a2200301 a 4500 001 1058727 005 2018-07-22 008 2018 bl uuuu u00u1 u #d 020 $a978-94-62611-95-5 022 $a0567-7572 (print) / 2406-6168 (electronic) 024 7 $a10.17660/ActaHortic.2018.1199.2$2DOI 100 1 $aPEREIRA, J. 245 $aOriginal olive genotypes found in Uruguay identified by morphological and molecular markers.$h[electronic resource] 260 $c2018 490 $a(Acta Horticulturae; 1199) 500 $aIn: Acta Horticulturae (ISHS) 1199: VIII International Olive Symposium. Editors: S. Perica, G. Vuletin Selak, T. Klepo, L. Ferguson, L. Sebastiani. 520 $aAbstract Montevideo, the capital city of Uruguay, founded by Spaniards in the 18th century, and Colonia del Sacramento, established by the Portuguese in the 17th century, are sources of olive diversity. A study and analysis of this unexplored heritage was initiated 10 years ago, using morphological and molecular tools. After a primary geographical and historical survey, 360 Olea europaea fruit and leaf samples were collected. After morphological analysis of 11 stone characters, the samples could not be matched with any cultivars in databases from Mediterranean countries. When a set of 14 pairs of microsatellite primers was applied, nine different multilocus genotypes were detected that were not recorded in the databases. In agreement with the colonization process and olive tree introduction routes into South America, two groups of genotypes were distinguished: a) seven ?genetic mosaic? genotypes related to genotypes from Spain and Portugal, introduced to Uruguay in areas under Spanish control, and b) two genotypes with one of two alleles in less than 70% with molecular variants found in Hispanic and Portuguese cultivars. Because of their height and trunk size, these trees were assumed to be older. Based on this information, we conclude that Uruguay has preserved a unique and original gene pool, currently productive and adapted to local soil and climatic conditions. This pool is a heritage of global interest, suitable for commercial and cultural purposes. @ International Society for Horticultural Science. 650 $aOLIVOS 650 $aURUGUAY 653 $aANCIENT OLIVE TREES 653 $aEVOLUTION 653 $aMOLECULAR 653 $aMORPHOLOGICAL MARKERS 700 1 $aBERNAL, J. 700 1 $aMARTINELLI, L. 700 1 $aVILLAMIL, J.J. 700 1 $aCONDE, P. 773 $tActa Horticulturae, May 2018, N° 1199, p. 7-13.
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