| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha : |
09/06/2021 |
|
Actualizado : |
28/07/2023 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
CASTELLI L.; BRANCHICCELA, B.; ROMERO H.; ZUNINO P.; ANTUNEZ, K. |
|
Afiliación : |
LORELEY CASTELLI, Laboratorio de Microbiología Y Salud de Las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, Montevideo, Uruguay.; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HÉCTOR ROMERO, Departamento de Ecología Y Evolución, Facultad de Ciencias, Laboratorio de Organización Y Evolución del Genoma, Montevideo, Uruguay,; PABLO ZUNINO, Laboratorio de Microbiología Y Salud de Las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, Montevideo, Uruguay.; KARINA ANTUNEZ, Laboratorio de Microbiología Y Salud de Las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, Montevideo, Uruguay. |
|
Título : |
Seasonal Dynamics of the Honey Bee Gut Microbiota in Colonies Under Subtropical Climate: Seasonal Dynamics of Honey Bee Gut Microbiota. |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Microbial Ecology, 2022, volume 83, issue 2, p. 492-500. https://doi.org/10.1007/s00248-021-01756-1 |
|
ISSN : |
0095-3628. |
|
DOI : |
10.1007/s00248-021-01756-1 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received: 20 October 2020 / Accepted: 15 April 2021 / Published online: 11 May 2021. -- Partial fnancial support was received from ANII (POS_NAC_2013_1_12228) and PEDECIBA (Uruguay). |
|
Contenido : |
Abstract:Honey bees (Apis mellifera) provide invaluable benefts for food production and maintenance of biodiversity of natural environments through pollination. They are widely spread across the world, being adapted to diferent climatic conditions. To survive the winter in cold temperate regions, honey bees developed diferent strategies including storage of honey and pollen, confnement of individuals during the winter, and an annual cycle of colony growth and reproduction. Under these conditions, winter honey bees experience physiological changes, including changes in immunity and the composition of honey
bee gut microbiota. However, under tropical or subtropical climates, the life cycle can experience alterations, i.e., queens lay eggs during almost all the year and new honey bees emerge constantly. In the present study, we characterized nurses? honey bee gut microbiota in colonies under subtropical region through a year, combining qPCR, PCR-DGGE, and 16S rDNA highthroughput sequencing. We also identifed environmental variables involved in those changes. Our results showed that under the mentioned conditions, the number of bacteria is stable throughout the year. Diversity of gut microbiota is higher in spring
and lower in summer and winter. Gradual changes in compositions occur between seasons: Lactobacillus spp. predominate in spring while Gilliamella apicola and Snodgrasella alvi predominate in summer and winter. Environmental variables (mainly precipitations) afected the composition of the honey bee gut microbiota. Our fndings provide new insights into the dynamics of honey bee gut microbiota and may be useful to understand the adaptation of bees to diferent environmental conditions. MenosAbstract:Honey bees (Apis mellifera) provide invaluable benefts for food production and maintenance of biodiversity of natural environments through pollination. They are widely spread across the world, being adapted to diferent climatic conditions. To survive the winter in cold temperate regions, honey bees developed diferent strategies including storage of honey and pollen, confnement of individuals during the winter, and an annual cycle of colony growth and reproduction. Under these conditions, winter honey bees experience physiological changes, including changes in immunity and the composition of honey
bee gut microbiota. However, under tropical or subtropical climates, the life cycle can experience alterations, i.e., queens lay eggs during almost all the year and new honey bees emerge constantly. In the present study, we characterized nurses? honey bee gut microbiota in colonies under subtropical region through a year, combining qPCR, PCR-DGGE, and 16S rDNA highthroughput sequencing. We also identifed environmental variables involved in those changes. Our results showed that under the mentioned conditions, the number of bacteria is stable throughout the year. Diversity of gut microbiota is higher in spring
and lower in summer and winter. Gradual changes in compositions occur between seasons: Lactobacillus spp. predominate in spring while Gilliamella apicola and Snodgrasella alvi predominate in summer and winter. Environmental variables (mainly precipitations) afected t... Presentar Todo |
|
Palabras claves : |
High-throughput sequencing; Honey bee gut microbiota; HONEY BEES; PCR-DGGE. |
|
Thesagro : |
ABEJA MELIFERA; APICULTURA. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 02796naa a2200277 a 4500
001 1062119
005 2023-07-28
008 2022 bl uuuu u00u1 u #d
022 $a0095-3628.
024 7 $a10.1007/s00248-021-01756-1$2DOI
100 1 $aCASTELLI L.
245 $aSeasonal Dynamics of the Honey Bee Gut Microbiota in Colonies Under Subtropical Climate$bSeasonal Dynamics of Honey Bee Gut Microbiota.$h[electronic resource]
260 $c2022
500 $aArticle history: Received: 20 October 2020 / Accepted: 15 April 2021 / Published online: 11 May 2021. -- Partial fnancial support was received from ANII (POS_NAC_2013_1_12228) and PEDECIBA (Uruguay).
520 $aAbstract:Honey bees (Apis mellifera) provide invaluable benefts for food production and maintenance of biodiversity of natural environments through pollination. They are widely spread across the world, being adapted to diferent climatic conditions. To survive the winter in cold temperate regions, honey bees developed diferent strategies including storage of honey and pollen, confnement of individuals during the winter, and an annual cycle of colony growth and reproduction. Under these conditions, winter honey bees experience physiological changes, including changes in immunity and the composition of honey bee gut microbiota. However, under tropical or subtropical climates, the life cycle can experience alterations, i.e., queens lay eggs during almost all the year and new honey bees emerge constantly. In the present study, we characterized nurses? honey bee gut microbiota in colonies under subtropical region through a year, combining qPCR, PCR-DGGE, and 16S rDNA highthroughput sequencing. We also identifed environmental variables involved in those changes. Our results showed that under the mentioned conditions, the number of bacteria is stable throughout the year. Diversity of gut microbiota is higher in spring and lower in summer and winter. Gradual changes in compositions occur between seasons: Lactobacillus spp. predominate in spring while Gilliamella apicola and Snodgrasella alvi predominate in summer and winter. Environmental variables (mainly precipitations) afected the composition of the honey bee gut microbiota. Our fndings provide new insights into the dynamics of honey bee gut microbiota and may be useful to understand the adaptation of bees to diferent environmental conditions.
650 $aABEJA MELIFERA
650 $aAPICULTURA
653 $aHigh-throughput sequencing
653 $aHoney bee gut microbiota
653 $aHONEY BEES
653 $aPCR-DGGE
700 1 $aBRANCHICCELA, B.
700 1 $aROMERO H.
700 1 $aZUNINO P.
700 1 $aANTUNEZ, K.
773 $tMicrobial Ecology, 2022, volume 83, issue 2, p. 492-500. https://doi.org/10.1007/s00248-021-01756-1
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Treinta y Tres. |
|
Fecha actual : |
14/01/2022 |
|
Actualizado : |
14/01/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
NÚÑEZ OLIVERA, R.; BÓ, G.A.; MENCHACA, A. |
|
Afiliación : |
R. NÚÑEZ OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo.; G.A. BÓ, Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina. // Instituto A.P. de Ciencias Básicas y Aplicadas, Medicina Veterinaria, Universidad Nacional de Villa María. Córdoba, Argentina.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Association between length of proestrus, follicular size, estrus behavior, and pregnancy rate in beef heifers subjected to fixedetime artificial insemination. |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Theriogenology, 15 March 2022, volume 181, pages 1-7. doi: https://doi.org/10.1016/j.theriogenology.2021.12.028 |
|
DOI : |
10.1016/j.theriogenology.2021.12.028 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 9 June 2021 / Received in revised form 22 December 2021 / Accepted 25 December 2021 / Available online 31 December 2021. |
|
Contenido : |
This study evaluated the relationship between proestrus length and follicular size, estrous behavior, and pregnancy rate in Bos taurus beef heifers subjected to ixedetime artificial insemination (FTAI). A total of 911 heifers received a synchronization treatment protocol for FTAI (JeSynch) consisting of an intravaginal
progesterone device for 6 d, estradiol benzoate at the time of device insertion cloprostenol sodium and eCG at device removal and GnRH at the time of FTAI. The presence or absence of a corpus luteum (CL) was determined by ultrasonography at device insertion and all heifers were tail painted at device removal for estrus detection at the time of FTAI. For the establishment of different periods of proestrus length (i.e., interval from device removal to FTAI), GnRH was administered i.m. at 48 h (n ¼ 308), 60 h (n ¼ 290) or 72 h (n ¼ 313) after device removal. The diameter of the largest follicle at the time of GnRH administrationwas determined by ultrasonography, expression of estrous was determined by percentage of tail paint removal, and FTAI was performed at the time of GnRH administration in all heifers. The diameter of the largest follicle was greater when GnRH/FTAI was performed at 72 or 60 h (12.9 ± 0.2 mm and 12.8 ± 0.1 mm, respectively) than at 48 h (12.2 ± 0.1 mm, P < 0.05). The proportion of heifers in estrus tended to be greater when GnRH/FTAI was performed at 72 h (77.0%, 137/178) than at 48 h (68.2%,
122/179; P ¼ 0.06), and intermediate at 60 h (71.4%, 120/168). Pregnancy rate tended to be greater in heifers with the longest (72 h: 70.0%, 219/313) than the shortest (48 h: 63.6%, 196/308; P < 0.1) proestrus length, while 60 h proestrus length was intermediate (63.1%, 183/290; P¼ NS). Pregnancy rate was
affected by the presence of a CL at device insertion (71.3%, 352/494 in heifers with a CL, vs. 59.0%, 246/417 for those without a CL; P < 0.01). For those heifers bearing a CL, pregnancy rate was greater in heifers with a 72 h proestrus length (77.0%, 134/174) than with 48 or 60 h proestrus length (67.7%, 107/158 and
68.5%, 111/162; respectively; P < 0.05). In heifers without a CL, proestrus length did not affect pregnancy rate. In summary, extending proestrus length by delaying the interval from device removal to GnRH/FTAI from 48 to 72 h, was associated with a greater diameter of the preovulatory follicle, greater proportion of heifers expressing estrus at the time of FTAI, and greater pregnancy rate in cycling beef heifers. MenosThis study evaluated the relationship between proestrus length and follicular size, estrous behavior, and pregnancy rate in Bos taurus beef heifers subjected to ixedetime artificial insemination (FTAI). A total of 911 heifers received a synchronization treatment protocol for FTAI (JeSynch) consisting of an intravaginal
progesterone device for 6 d, estradiol benzoate at the time of device insertion cloprostenol sodium and eCG at device removal and GnRH at the time of FTAI. The presence or absence of a corpus luteum (CL) was determined by ultrasonography at device insertion and all heifers were tail painted at device removal for estrus detection at the time of FTAI. For the establishment of different periods of proestrus length (i.e., interval from device removal to FTAI), GnRH was administered i.m. at 48 h (n ¼ 308), 60 h (n ¼ 290) or 72 h (n ¼ 313) after device removal. The diameter of the largest follicle at the time of GnRH administrationwas determined by ultrasonography, expression of estrous was determined by percentage of tail paint removal, and FTAI was performed at the time of GnRH administration in all heifers. The diameter of the largest follicle was greater when GnRH/FTAI was performed at 72 or 60 h (12.9 ± 0.2 mm and 12.8 ± 0.1 mm, respectively) than at 48 h (12.2 ± 0.1 mm, P < 0.05). The proportion of heifers in estrus tended to be greater when GnRH/FTAI was performed at 72 h (77.0%, 137/178) than at 48 h (68.2%,
122/179; P ¼ 0.06), and intermediate at 60 h (71.4... Presentar Todo |
|
Palabras claves : |
FOLÍCULO; FOLLICLE; OVULATION; TIMED ARTIFICAL INSEMINATION; ULTRASONOGRAFIA; ULTRASONOGRAPHY. |
|
Asunto categoría : |
L53 Fisiología Animal - Reproducción |
|
Marc : |
LEADER 03484naa a2200241 a 4500
001 1062633
005 2022-01-14
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2021.12.028$2DOI
100 1 $aNÚÑEZ OLIVERA, R.
245 $aAssociation between length of proestrus, follicular size, estrus behavior, and pregnancy rate in beef heifers subjected to fixedetime artificial insemination.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 9 June 2021 / Received in revised form 22 December 2021 / Accepted 25 December 2021 / Available online 31 December 2021.
520 $aThis study evaluated the relationship between proestrus length and follicular size, estrous behavior, and pregnancy rate in Bos taurus beef heifers subjected to ixedetime artificial insemination (FTAI). A total of 911 heifers received a synchronization treatment protocol for FTAI (JeSynch) consisting of an intravaginal progesterone device for 6 d, estradiol benzoate at the time of device insertion cloprostenol sodium and eCG at device removal and GnRH at the time of FTAI. The presence or absence of a corpus luteum (CL) was determined by ultrasonography at device insertion and all heifers were tail painted at device removal for estrus detection at the time of FTAI. For the establishment of different periods of proestrus length (i.e., interval from device removal to FTAI), GnRH was administered i.m. at 48 h (n ¼ 308), 60 h (n ¼ 290) or 72 h (n ¼ 313) after device removal. The diameter of the largest follicle at the time of GnRH administrationwas determined by ultrasonography, expression of estrous was determined by percentage of tail paint removal, and FTAI was performed at the time of GnRH administration in all heifers. The diameter of the largest follicle was greater when GnRH/FTAI was performed at 72 or 60 h (12.9 ± 0.2 mm and 12.8 ± 0.1 mm, respectively) than at 48 h (12.2 ± 0.1 mm, P < 0.05). The proportion of heifers in estrus tended to be greater when GnRH/FTAI was performed at 72 h (77.0%, 137/178) than at 48 h (68.2%, 122/179; P ¼ 0.06), and intermediate at 60 h (71.4%, 120/168). Pregnancy rate tended to be greater in heifers with the longest (72 h: 70.0%, 219/313) than the shortest (48 h: 63.6%, 196/308; P < 0.1) proestrus length, while 60 h proestrus length was intermediate (63.1%, 183/290; P¼ NS). Pregnancy rate was affected by the presence of a CL at device insertion (71.3%, 352/494 in heifers with a CL, vs. 59.0%, 246/417 for those without a CL; P < 0.01). For those heifers bearing a CL, pregnancy rate was greater in heifers with a 72 h proestrus length (77.0%, 134/174) than with 48 or 60 h proestrus length (67.7%, 107/158 and 68.5%, 111/162; respectively; P < 0.05). In heifers without a CL, proestrus length did not affect pregnancy rate. In summary, extending proestrus length by delaying the interval from device removal to GnRH/FTAI from 48 to 72 h, was associated with a greater diameter of the preovulatory follicle, greater proportion of heifers expressing estrus at the time of FTAI, and greater pregnancy rate in cycling beef heifers.
653 $aFOLÍCULO
653 $aFOLLICLE
653 $aOVULATION
653 $aTIMED ARTIFICAL INSEMINATION
653 $aULTRASONOGRAFIA
653 $aULTRASONOGRAPHY
700 1 $aBÓ, G.A.
700 1 $aMENCHACA, A.
773 $tTheriogenology, 15 March 2022, volume 181, pages 1-7. doi: https://doi.org/10.1016/j.theriogenology.2021.12.028
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Treinta y Tres (TT) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
