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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
23/07/2021 |
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Actualizado : |
23/07/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OLIVARO, C.; ESCOBAL, M.; DE SOUZA, G.; MEDEROS, A. |
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Afiliación : |
CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembó, Uruguay; MICAELA ESCOBAL, Espacio de Ciencia y Tecnología Química, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembó, Uruguay; GUILLERMO DE SOUZA, Espacio de Ciencia y Tecnología Química, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembó, Uruguay; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Chemical characterisation and in vitro anthelmintic activity of phenolic-rich extracts from the leaves and branches of Maytenus ilicifolia, a native plant from South America. |
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Complemento del título : |
Short Communication. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Natural Product Research, 2021. Doi: https://doi.org/10.1080/14786419.2021.1948844 |
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DOI : |
10.1080/14786419.2021.1948844 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 Mar 2021, Accepted 20 Jun 2021, Published online: 06 Jul 2021.
Correspondence: Cristina Olivaro, emai: cristina.olivaro@cut.edu.uy -
Supplemental data for this article can be accessed online at https://www.tandfonline.com/doi/suppl/10.1080/14786419.2021.1948844
This work was supported by the INIA under Grant INIA CL_40 and PEDECIBA. Instituto Nacional de Investigación Agropecuaria (INIA); Programa de Desarrollo de las Ciencias Básicas (PEDECIBA). |
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Contenido : |
ABSTRACT - Free (epi) catechin, quercetin, (epi) gallocatechin, flavonol glycosides and condensed tannins were identified according to their molecular mass, characteristic product ions and retention times in extracts obtained from leaves and branches of Maytenus ilicifolia (Congorosa) by mass spectrometry. The in vitro anthelmintic activity against cattle gastrointestinal nematodes of Congorosa extract was determined using the Egg Hatch Inhibition Assay. Additionally, commercial quercetin, gallocatechin and epicatechin were evaluated. Although total phenolics, total tannins and condensed tannins contents were lower in branches extract than in leaves extract, the EC50 were 0.065mg/mL and 0.890 mg/mL for branches and leaves extract, respectively. Moreover, the use of polyvinylpyrrolidone as a blocking agent of tannins, did not change significantly the EC50 for branches extract, but significantly changed for leaves extract. Quercetin and gallocatechin EC50 values were in the range 0.03–0.05 mg/mL and epicatechin showed 100% inhibition of the egg hatching process at 0.004mg/mL.
Copyright © 2021 Informa UK Limited, trading as Taylor & Francis Group |
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Palabras claves : |
Egg hatch inhibition assay; Mass spectrometry; Maytenus ilicifolia; Phenolic compounds; Ruminants anthelmintic activity. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02502naa a2200241 a 4500
001 1062322
005 2021-07-23
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1080/14786419.2021.1948844$2DOI
100 1 $aOLIVARO, C.
245 $aChemical characterisation and in vitro anthelmintic activity of phenolic-rich extracts from the leaves and branches of Maytenus ilicifolia, a native plant from South America.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 28 Mar 2021, Accepted 20 Jun 2021, Published online: 06 Jul 2021. Correspondence: Cristina Olivaro, emai: cristina.olivaro@cut.edu.uy - Supplemental data for this article can be accessed online at https://www.tandfonline.com/doi/suppl/10.1080/14786419.2021.1948844 This work was supported by the INIA under Grant INIA CL_40 and PEDECIBA. Instituto Nacional de Investigación Agropecuaria (INIA); Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).
520 $aABSTRACT - Free (epi) catechin, quercetin, (epi) gallocatechin, flavonol glycosides and condensed tannins were identified according to their molecular mass, characteristic product ions and retention times in extracts obtained from leaves and branches of Maytenus ilicifolia (Congorosa) by mass spectrometry. The in vitro anthelmintic activity against cattle gastrointestinal nematodes of Congorosa extract was determined using the Egg Hatch Inhibition Assay. Additionally, commercial quercetin, gallocatechin and epicatechin were evaluated. Although total phenolics, total tannins and condensed tannins contents were lower in branches extract than in leaves extract, the EC50 were 0.065mg/mL and 0.890 mg/mL for branches and leaves extract, respectively. Moreover, the use of polyvinylpyrrolidone as a blocking agent of tannins, did not change significantly the EC50 for branches extract, but significantly changed for leaves extract. Quercetin and gallocatechin EC50 values were in the range 0.03–0.05 mg/mL and epicatechin showed 100% inhibition of the egg hatching process at 0.004mg/mL. Copyright © 2021 Informa UK Limited, trading as Taylor & Francis Group
653 $aEgg hatch inhibition assay
653 $aMass spectrometry
653 $aMaytenus ilicifolia
653 $aPhenolic compounds
653 $aRuminants anthelmintic activity
700 1 $aESCOBAL, M.
700 1 $aDE SOUZA, G.
700 1 $aMEDEROS, A.
773 $tNatural Product Research, 2021. Doi: https://doi.org/10.1080/14786419.2021.1948844
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha actual : |
24/09/2014 |
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Actualizado : |
08/08/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BATISTA, L.; IRISARRI, L.; REBUFFO, M.; CUITIÑO, M.J.; SANJUÁN, J.; MONZA , J. |
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Afiliación : |
LETICIA BATISTA, Universidad de la República (UdelaR)/ Facultad de Agronomía; PILAR IRISARRI, Universidad de la República (UdelaR)/ Facultad de Agronomía; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA JOSE CUITIÑO DE VEGA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN SANJUÁN, Estación Experimental del Zaidín; JORGE MONZA, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
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Título : |
Nodulation competitiveness as a requisite for improved rhizobial inoculants of Trifolium pratense. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Biology and Fertility of Soils , v. 51, n. 1, p. 11-20, 2015 |
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ISSN : |
0178-2762. |
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DOI : |
10.1007/s00374-014-0946-3 |
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Idioma : |
Inglés |
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Notas : |
Received: 21 April 2014 /Revised: 14 July 2014 /Accepted: 16 July 2014 /Published online: 27 July 2014 |
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Contenido : |
ABSTRACT.
Red clover (Trifolium pratense L.) is used in the improvement of grasslands in Uruguay and has been inoculated with commercial strain U204 of Rhizobium leguminosarum bv trifolii since 1970s. Native-naturalized rhizobia strains present in soil are the basis for selecting and developing new inoculants. With this aim, we evaluated the diversity of red clover rhizobia in Uruguayan red clover pastures both historically inoculated with U204 and noninoculated ones. Thirty-eight different enterobacterial repetitive intergenic consensus (ERIC) PCR genomic fingerprints were identified, albeit surprisingly only one of 80 isolates showed an ERIC profile similar to U204. Under controlled conditions, red clover plants inoculated with one of the native isolates, strain 317, produced more biomass than those inoculated with the commercial U204. ERIC-PCR was also used to show that strain 317 competed for nodulation better than U204 in a field with previous history of inoculation. Moreover, both U204 and 317 were tagged with a gusA reporter gene and their competitiveness for nodulation assessed in various soil types. Again, strain 317 appeared more competitive than U204, particularly in soils with previous history of inoculation. Our results reinforce the long-known idea of assessing the actual needs of inoculation of legumes in different soils and suggest that the indigenous isolate 317 is an effective and competitive strain that can be used for development of a new red clover inoculant.
@ Springer-Verlag Berlin Heidelberg 2014 MenosABSTRACT.
Red clover (Trifolium pratense L.) is used in the improvement of grasslands in Uruguay and has been inoculated with commercial strain U204 of Rhizobium leguminosarum bv trifolii since 1970s. Native-naturalized rhizobia strains present in soil are the basis for selecting and developing new inoculants. With this aim, we evaluated the diversity of red clover rhizobia in Uruguayan red clover pastures both historically inoculated with U204 and noninoculated ones. Thirty-eight different enterobacterial repetitive intergenic consensus (ERIC) PCR genomic fingerprints were identified, albeit surprisingly only one of 80 isolates showed an ERIC profile similar to U204. Under controlled conditions, red clover plants inoculated with one of the native isolates, strain 317, produced more biomass than those inoculated with the commercial U204. ERIC-PCR was also used to show that strain 317 competed for nodulation better than U204 in a field with previous history of inoculation. Moreover, both U204 and 317 were tagged with a gusA reporter gene and their competitiveness for nodulation assessed in various soil types. Again, strain 317 appeared more competitive than U204, particularly in soils with previous history of inoculation. Our results reinforce the long-known idea of assessing the actual needs of inoculation of legumes in different soils and suggest that the indigenous isolate 317 is an effective and competitive strain that can be used for development of a new red clover inoc... Presentar Todo |
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Palabras claves : |
DNA FINGERPRINTING; GusA; RED CLOVER; RHIZOBIUM LEGUMINOSARUM; RHIZOBIUM LEGUMINOSARUM BV TRIFOLI; TRÉBOL ROJO; TRIFOLIUM PRATENSE. |
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Thesagro : |
MEJORAMIENTO DE LEGUMINOSAS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02569naa a2200313 a 4500
001 1054070
005 2017-08-08
008 2015 bl uuuu u00u1 u #d
022 $a0178-2762.
024 7 $a10.1007/s00374-014-0946-3$2DOI
100 1 $aBATISTA, L.
245 $aNodulation competitiveness as a requisite for improved rhizobial inoculants of Trifolium pratense.$h[electronic resource]
260 $c2015
500 $aReceived: 21 April 2014 /Revised: 14 July 2014 /Accepted: 16 July 2014 /Published online: 27 July 2014
520 $aABSTRACT. Red clover (Trifolium pratense L.) is used in the improvement of grasslands in Uruguay and has been inoculated with commercial strain U204 of Rhizobium leguminosarum bv trifolii since 1970s. Native-naturalized rhizobia strains present in soil are the basis for selecting and developing new inoculants. With this aim, we evaluated the diversity of red clover rhizobia in Uruguayan red clover pastures both historically inoculated with U204 and noninoculated ones. Thirty-eight different enterobacterial repetitive intergenic consensus (ERIC) PCR genomic fingerprints were identified, albeit surprisingly only one of 80 isolates showed an ERIC profile similar to U204. Under controlled conditions, red clover plants inoculated with one of the native isolates, strain 317, produced more biomass than those inoculated with the commercial U204. ERIC-PCR was also used to show that strain 317 competed for nodulation better than U204 in a field with previous history of inoculation. Moreover, both U204 and 317 were tagged with a gusA reporter gene and their competitiveness for nodulation assessed in various soil types. Again, strain 317 appeared more competitive than U204, particularly in soils with previous history of inoculation. Our results reinforce the long-known idea of assessing the actual needs of inoculation of legumes in different soils and suggest that the indigenous isolate 317 is an effective and competitive strain that can be used for development of a new red clover inoculant. @ Springer-Verlag Berlin Heidelberg 2014
650 $aMEJORAMIENTO DE LEGUMINOSAS
653 $aDNA FINGERPRINTING
653 $aGusA
653 $aRED CLOVER
653 $aRHIZOBIUM LEGUMINOSARUM
653 $aRHIZOBIUM LEGUMINOSARUM BV TRIFOLI
653 $aTRÉBOL ROJO
653 $aTRIFOLIUM PRATENSE
700 1 $aIRISARRI, L.
700 1 $aREBUFFO, M.
700 1 $aCUITIÑO, M.J.
700 1 $aSANJUÁN, J.
700 1 $aMONZA , J.
773 $tBiology and Fertility of Soils$gv. 51, n. 1, p. 11-20, 2015
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