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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
01/11/2021 |
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Actualizado : |
01/11/2021 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
FERREIRA, V.; GONZÁLEZ-ARCOS, M.; PIANZZOLA, M.J.; COLL, N.S.; SIRI, M.I.; VALLS, M. |
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Afiliación : |
VIRGINIA FERREIRA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JULIA PIANZZOLA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; NÚRIA S. COLL, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; MARÍA INÉS SIRI, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARC VALLS, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; Department of Genetics, University of Barcelona, Bellaterra, Catalonia, Spain. |
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Título : |
Molecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
In: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18 |
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Serie : |
eBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354). |
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ISBN : |
978-1-0716-1608-6 (print) / 978-1-0716-1609-3 (e-book) |
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ISSN : |
1064-3745 (print) / 1940-6029 (electronic) |
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DOI : |
10.1007/978-1-0716-1609-3_18 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Online 27 August 2021. |
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Contenido : |
ABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature. |
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Palabras claves : |
Bacterial wilt; Disease resistance; Plant breeding; Potato brown rot; Ralstonia solanacearum; Solanum tuberosum. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02607naa a2200301 a 4500
001 1062509
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008 2021 bl uuuu u00u1 u #d
022 $a1064-3745 (print) / 1940-6029 (electronic)
024 7 $a10.1007/978-1-0716-1609-3_18$2DOI
100 1 $aFERREIRA, V.
245 $aMolecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato.$h[electronic resource]
260 $c2021
490 $aeBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354).
500 $aArticle history: First Online 27 August 2021.
520 $aABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
653 $aBacterial wilt
653 $aDisease resistance
653 $aPlant breeding
653 $aPotato brown rot
653 $aRalstonia solanacearum
653 $aSolanum tuberosum
700 1 $aGONZÁLEZ-ARCOS, M.
700 1 $aPIANZZOLA, M.J.
700 1 $aCOLL, N.S.
700 1 $aSIRI, M.I.
700 1 $aVALLS, M.
773 $tIn: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
12/09/2016 |
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Actualizado : |
20/09/2016 |
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Tipo de producción científica : |
Presentaciones Orales |
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Autor : |
PIAGGIO, L.; BANCHERO, G.; DESCHENAUX, H.; GONZÁLEZ, D. |
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Afiliación : |
LUCIA PIAGGIO, SUL (Secretariado Uruguayo de Lana), Uruguay.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Principales aspectos para la implementación de la suplementación con alimentos concentrados en ovinos en pastoreo. [Presentación oral]. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
In: REUNIÓN INTERNA INIA-SUL PARA FACILIAR LA INTERACCIÓN DE LOS INVESTIGADORES INVOLUCRADOS EN LA GENERACIÓN DE INFORMACIÓN NACIONAL. INIA La Estanzuela, Colonia: INIA, 4 de Mayo de 2016. |
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Idioma : |
Español |
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Palabras claves : |
SUPLEMENTACIÓN CON ALIMENTOS CONCENTRADOS. |
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Thesagro : |
SUPLEMENTACIÓN. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 00726nam a2200157 a 4500
001 1055606
005 2016-09-20
008 2016 bl uuuu u00u1 u #d
100 1 $aPIAGGIO, L.
245 $aPrincipales aspectos para la implementación de la suplementación con alimentos concentrados en ovinos en pastoreo. [Presentación oral].$h[electronic resource]
260 $aIn: REUNIÓN INTERNA INIA-SUL PARA FACILIAR LA INTERACCIÓN DE LOS INVESTIGADORES INVOLUCRADOS EN LA GENERACIÓN DE INFORMACIÓN NACIONAL. INIA La Estanzuela, Colonia: INIA, 4 de Mayo de 2016.$c2016
650 $aSUPLEMENTACIÓN
653 $aSUPLEMENTACIÓN CON ALIMENTOS CONCENTRADOS
700 1 $aBANCHERO, G.
700 1 $aDESCHENAUX, H.
700 1 $aGONZÁLEZ, D.
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