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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
27/06/2023 |
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Actualizado : |
28/06/2023 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
DE TRAVENSOLO, R.F.; FERREIRA, V.G.; FEDERICI, M.; DE LEMOS, E.G.M.; CARRILHO, E. |
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Afiliación : |
REGIANE FÁTIMA DE TRAVENSOLO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; VINÍCIUS GUIMARÃES FERREIRA, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELIANA GERTRUDES MACEDO DE LEMOS, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, Jaboticabal, SP, Brazil; EMANUEL CARRILHO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil. |
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Título : |
Microarrays application in life sciences: The beginning of the revolution. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
In: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25 |
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ISBN : |
978-303082380-1; eISBN: 978-303082381-8 |
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DOI : |
10.1007/978-3-030-82381-8_25 |
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Idioma : |
Inglés |
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Notas : |
Correspondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br -- |
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Contenido : |
Previous to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd. |
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Palabras claves : |
DNA analysis; Microarray applications; Microarrays. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 01965naa a2200229 a 4500
001 1064209
005 2023-06-28
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-3-030-82381-8_25$2DOI
100 1 $aDE TRAVENSOLO, R.F.
245 $aMicroarrays application in life sciences$bThe beginning of the revolution.$h[electronic resource]
260 $c2022
500 $aCorrespondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br --
520 $aPrevious to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd.
653 $aDNA analysis
653 $aMicroarray applications
653 $aMicroarrays
700 1 $aFERREIRA, V.G.
700 1 $aFEDERICI, M.
700 1 $aDE LEMOS, E.G.M.
700 1 $aCARRILHO, E.
773 $tIn: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25
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Registro original : |
INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
10/09/2014 |
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Actualizado : |
04/10/2019 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
UMPIÉRREZ M.; GARMENDIA G.; CABRERA M.; PEREYRA, S.; VERO, S. |
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Afiliación : |
SILVIA ANTONIA PEREYRA CORREA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Diversity of pathogen populations causing Fusarium head blight of wheat in Uruguay. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
In ALCONADA MAGLIANO, T.M.; CHULZE, S.N. (Ed.). Fusarium Head Blight in Latin America. Dordrecht, NL: Springer, 2013. p. 31-44, 2013 |
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ISBN : |
978-94-007-7091-1 |
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DOI : |
10.1007/978-94-007-7091-1_3 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Online: 01 August 2013. |
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Contenido : |
ABSTRACT.
Members of the Fusarium graminearum species complex (FGSC) are the primary pathogens causing Fusarium head blight (FHB), Fusarium graminearum is the main pathogen of FHB and can produce various mycotoxins in wheat, in particular type B trichothecenes. This review presents information on the Fusarium species and chemotypes diversity associated to FHB in wheat in Uruguay. In surveys performed in 2001 and 2002, Fusarium graminearum was the most frequently isolated species (76 %), while other non FGSC species were also identified. Among species from FGSC Fusarium graminearum sensu stricto was the most frequently isolated (97 %), while F. cortaderiae and F. austroamericanum were also identified. The predominant chemotype was 15ADON (95 %), followed by 3ADON (3 %) and NIV (2 %). Isolates identified as F. graminearum sensu stricto were characterized according to the levels of DON production, the aggressiveness on different wheat cultivars, the ability to form perithecia on wheat straw. The sensitiveness to the fungicide tebuconazole of FGSC isolates was evaluated. Most isolates showed high sensitivity levels, but a few resisted higher levels of fungicide. Lower sensitivity seemed to be tied to species since F. cortaderiae isolates showed the highest MIC values. |
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Palabras claves : |
AGRESSIVENESS; FUSARIUM ASIATICUM; FUSARIUM AUSTROAMERICANUM; FUSARIUM BRASILICUM; FUSARIUM CORTADERIAE; FUSARIUM GRAMINEARUM; FUSARIUM GRAMINEARUM SPECIES COMPLEX; MYCOTOXINS; TRITCUM. |
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Thesagro : |
CULTIVOS DE INVIERNO; FITOPATOLOGÍA. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02390naa a2200337 a 4500
001 1050097
005 2019-10-04
008 2013 bl uuuu u00u1 u #d
020 $a978-94-007-7091-1
024 7 $a10.1007/978-94-007-7091-1_3$2DOI
100 1 $aUMPIÉRREZ M.
245 $aDiversity of pathogen populations causing Fusarium head blight of wheat in Uruguay.$h[electronic resource]
260 $c2013
500 $aArticle history: First Online: 01 August 2013.
520 $aABSTRACT. Members of the Fusarium graminearum species complex (FGSC) are the primary pathogens causing Fusarium head blight (FHB), Fusarium graminearum is the main pathogen of FHB and can produce various mycotoxins in wheat, in particular type B trichothecenes. This review presents information on the Fusarium species and chemotypes diversity associated to FHB in wheat in Uruguay. In surveys performed in 2001 and 2002, Fusarium graminearum was the most frequently isolated species (76 %), while other non FGSC species were also identified. Among species from FGSC Fusarium graminearum sensu stricto was the most frequently isolated (97 %), while F. cortaderiae and F. austroamericanum were also identified. The predominant chemotype was 15ADON (95 %), followed by 3ADON (3 %) and NIV (2 %). Isolates identified as F. graminearum sensu stricto were characterized according to the levels of DON production, the aggressiveness on different wheat cultivars, the ability to form perithecia on wheat straw. The sensitiveness to the fungicide tebuconazole of FGSC isolates was evaluated. Most isolates showed high sensitivity levels, but a few resisted higher levels of fungicide. Lower sensitivity seemed to be tied to species since F. cortaderiae isolates showed the highest MIC values.
650 $aCULTIVOS DE INVIERNO
650 $aFITOPATOLOGÍA
653 $aAGRESSIVENESS
653 $aFUSARIUM ASIATICUM
653 $aFUSARIUM AUSTROAMERICANUM
653 $aFUSARIUM BRASILICUM
653 $aFUSARIUM CORTADERIAE
653 $aFUSARIUM GRAMINEARUM
653 $aFUSARIUM GRAMINEARUM SPECIES COMPLEX
653 $aMYCOTOXINS
653 $aTRITCUM
700 1 $aGARMENDIA G.
700 1 $aCABRERA M.
700 1 $aPEREYRA, S.
700 1 $aVERO, S.
773 $tIn ALCONADA MAGLIANO, T.M.; CHULZE, S.N. (Ed.). Fusarium Head Blight in Latin America. Dordrecht, NL: Springer, 2013. p. 31-44, 2013
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