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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
27/06/2023 |
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Actualizado : |
28/06/2023 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
DE TRAVENSOLO, R.F.; FERREIRA, V.G.; FEDERICI, M.; DE LEMOS, E.G.M.; CARRILHO, E. |
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Afiliación : |
REGIANE FÁTIMA DE TRAVENSOLO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; VINÍCIUS GUIMARÃES FERREIRA, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELIANA GERTRUDES MACEDO DE LEMOS, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, Jaboticabal, SP, Brazil; EMANUEL CARRILHO, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil. |
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Título : |
Microarrays application in life sciences: The beginning of the revolution. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
In: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25 |
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ISBN : |
978-303082380-1; eISBN: 978-303082381-8 |
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DOI : |
10.1007/978-3-030-82381-8_25 |
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Idioma : |
Inglés |
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Notas : |
Correspondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br -- |
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Contenido : |
Previous to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd. |
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Palabras claves : |
DNA analysis; Microarray applications; Microarrays. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
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024 7 $a10.1007/978-3-030-82381-8_25$2DOI
100 1 $aDE TRAVENSOLO, R.F.
245 $aMicroarrays application in life sciences$bThe beginning of the revolution.$h[electronic resource]
260 $c2022
500 $aCorrespondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br --
520 $aPrevious to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd.
653 $aDNA analysis
653 $aMicroarray applications
653 $aMicroarrays
700 1 $aFERREIRA, V.G.
700 1 $aFEDERICI, M.
700 1 $aDE LEMOS, E.G.M.
700 1 $aCARRILHO, E.
773 $tIn: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25
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| 1. |  | LOURENCO, D.A.L.; FRAGOMENI, B.O.; BRADFORD, H.L.; MENEZES I.R.; FERRAZ, J.B.S.; AGUILAR, I.; MISZTAL, I. Implications of SNP weighting on single-step genomic predictions for different reference population sizes. Journal of Animal Breeding and Genetics, 2017, v. 134 (6), p. 463-471. Article history: Received: 28 February 2017 / Accepted: 19 July 2017.
This study was partially funded by the American Angus Association (St. Joseph, MO), Zoetis (Kalamazoo, MI) and by Agriculture and Food Research Initiative Competitive...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 2. | | BERTON, M. P.; DA SILVA, ROSIANE PEREIRA; BANCHERO, G.; MOURÃO, G. B.; FERRAZ, J. B. S.; SCHENKEL, F. S.; BALDI, F. Genomic integration to identify molecular biomarkers associated with indicator traits of gastrointestinal nematode resistance in sheep. Journal of Animal Breeding and Genetics, 2022, Volume 139, Issue 5, pages 502-516. doi: https://doi.org/10.1111/jbg.12682 Article history: Received 14 June 2021; Accepted 21 April 2022; First published 09 May 2022; Publisched online September 2022. -- Corresponding author: Berton, M.P.; Departamento de Zootecnia, Faculdade de Ciências Agrárias e...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
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| 3. | | SILVA, S.D.L.; TAROUCO, J.U.; Ferraz, J.B.S.; GOMES, R.D.C.; LEME, P.R.; NAVAJAS, E.A. Prediction of retail beef yield, trim fat and proportion of high-valued cuts in nellore cattle using ultrasound live measurements. Revista Brasileira de Zootecnia, 2012, vol.41, no.9, p.2025-2031. Article history: Received October 20, 2011 / Accepted May 10, 2012.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 1 |
| Biblioteca(s): INIA Las Brujas. |
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| 4. | | CARVALHO, F.E.; ESPIGOLAN, R.; BERTON, M.P.; NETO, J.B.S.; SILVA, R.P.; GRIGOLETTO, L.; SILVA, R.M.O.; FERRAZ, J.B.S.; ELER, J.P.; AGUILAR, I.; LÔBO, R.B.; BALDI, F. Genome-wide association study and predictive ability for growth traits in Nellore cattle. Livestock Science, January 2020, Volume 231, Article number 103861. OPEN ACCESS. Doi: https://doi.org/10.1016/j.livsci.2019.103861 Article history: Received 25 June 2019 / Revised 29 October 2019 / Accepted 4 November 2019 / Available online 6 November 2019.
Funding information: F.E. CARVALHO received a scholarship from Coordinating for the Improvement of Higher...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 5. | | BERTON, MP.; DE OLIVEIRA SILVA, R.M.; PERIPOLLI, E.; STAFUZZA, N.B.; FERNÁNDEZ, J.; SAURA, S.; VILLANUEVA, B.; TORO, M.A.; BANCHERO, G.; OLIVEIRA, P.S.; ELER, J.P.; BALDI, F.; FERRAZ, J.B.S. Genomic regions and pathways associated with resistance to gastrointestinal parasites in tropical sheep breed. Journal of Animal Science, 2017, v.95, suppl.4.p.107.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 5 | |
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