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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2023 |
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Actualizado : |
24/10/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PÉREZ-RUCHEL, A.; BRITOS, A.; ALVARADO, A.; FERNÁNDEZ-CIGANDA, S.; GADEYNE, F.; BUSTOS, M.; ZUNINO, P.; CAJARVILLE, C. |
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Afiliación : |
ANALÍA PÉREZ-RUCHEL, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; ALEJANDRO BRITOS, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; AMAYRI ALVARADO, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; FREDERIK GADEYNE, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; MARIANA BUSTOS, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; CECILIA CAJARVILLE, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay. |
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Título : |
Impact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Animal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763 |
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ISSN : |
0377-8401. |
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DOI : |
10.1016/j.anifeedsci.2023.115763 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com -- |
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Contenido : |
This work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V. MenosThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearanc... Presentar Todo |
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Palabras claves : |
Gas production; Microbial protein synthesis; Microbiome profile; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Protein degradation. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 04021naa a2200301 a 4500
001 1064355
005 2023-10-24
008 2023 bl uuuu u00u1 u #d
022 $a0377-8401.
024 7 $a10.1016/j.anifeedsci.2023.115763$2DOI
100 1 $aPÉREZ-RUCHEL, A.
245 $aImpact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com --
520 $aThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V.
653 $aGas production
653 $aMicrobial protein synthesis
653 $aMicrobiome profile
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aProtein degradation
700 1 $aBRITOS, A.
700 1 $aALVARADO, A.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aGADEYNE, F.
700 1 $aBUSTOS, M.
700 1 $aZUNINO, P.
700 1 $aCAJARVILLE, C.
773 $tAnimal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
13/02/2019 |
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Actualizado : |
23/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
RESQUÍN, F.; NAVARRO-CERRILLO, R.M.; CARRASCO-LETELIER, L.; RACHID, C. |
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Afiliación : |
JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RARAEL M. NAVARRO-CERRILLO, E.T.S.I.A.M.-Department of Forestry, School of Agriculture and Forestry, University of Córdoba, Córdoba, Spain.; LEONIDAS CARRASCO-LETELIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA CECILIA RACHID CASNATI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Influence of contrasting stocking densities on the dynamics of above-ground biomass and wood density of Eucalyptus benthamii, Eucalyptus dunnii, and Eucalyptus grandis for bioenergy in Uruguay. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Forest Ecology and Management, 2019, v. 438, p. 63-74. |
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DOI : |
10.1016/j.foreco.2019.02.007 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 September 2018; Received in revised form 31 January 2019; Accepted 4 February 2019. |
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Contenido : |
Short-rotation plantations have been suggested to develop renewable energies in South America. Our study evaluated the biomass production of Eucalyptus benthamii, E. dunnii and E. grandis plantations at stocking densities of 2220, 3330, 4440 and 6660 trees ha-1 in the North (Tacuarembó) and West (Paysandú) of Uruguay,
over a 76-month period. The species survival was not related to planting density, and the highest mortality rates occurred at Tacuarembó. The effects of tree competition were more evident for E. grandis. At Tacuarembó site, the average survival of the species were: 57, 57 and 46% at age 76 months for E. benthamii, E. dunnii and E. grandis, respectively. Survival rates were similar for the different planting densities, with ranges between 50 and 57%. At Paysandú site, the average survival of the species were: 83, 86 and 75% for E. benthamii, E. dunnii and E. grandis, respectively, with a range from 78 to 84% for different planting densities. Weather conditions (temperature and rainfall) were similar at both sites throughout the evaluation period. Wood density and individual and total biomass weight (kg tree-1 and Mg ha-1,respectively) were evaluated sampling destructively 2592 trees and logarithmic biomass equations were developed using the diameter at breast height (cm) and total height (m) of the trees. Wood density increased with the age of the crop only at Paysandú. On average, wood density increased from 0.405 g cm -3 at age 18 months to 0.497 g cm -3 at age 76 months. The highest wood density was observed at 76 months on E. benthamii (0.413 and 0.431 g cm -3) and E. dunnii (0.496 and 0.539 g cm -3) at Tacuarembó and Paysandú, respectively. Age had no effect on the wood density at Tacuarembó. The stem biomass showed an inversely-proportional relationship with the planting density. Individual stem biomass was higher for E. grandis (81 and 74 kg tree -1 on average at Tacuarembó and Paysandú, respectively) than for the other species. The highest biomass per hectare was achieved for the highest planting density, specifically for E. dunnii (193 Mg ha -1) and E. grandis (203 Mg ha -1) at Paysandú and for E. dunnii (157 Mg ha -1) at Tacuarembó. At both locations, the effects of contrasting planting densities on biomass production increased throughout the rotation. MenosShort-rotation plantations have been suggested to develop renewable energies in South America. Our study evaluated the biomass production of Eucalyptus benthamii, E. dunnii and E. grandis plantations at stocking densities of 2220, 3330, 4440 and 6660 trees ha-1 in the North (Tacuarembó) and West (Paysandú) of Uruguay,
over a 76-month period. The species survival was not related to planting density, and the highest mortality rates occurred at Tacuarembó. The effects of tree competition were more evident for E. grandis. At Tacuarembó site, the average survival of the species were: 57, 57 and 46% at age 76 months for E. benthamii, E. dunnii and E. grandis, respectively. Survival rates were similar for the different planting densities, with ranges between 50 and 57%. At Paysandú site, the average survival of the species were: 83, 86 and 75% for E. benthamii, E. dunnii and E. grandis, respectively, with a range from 78 to 84% for different planting densities. Weather conditions (temperature and rainfall) were similar at both sites throughout the evaluation period. Wood density and individual and total biomass weight (kg tree-1 and Mg ha-1,respectively) were evaluated sampling destructively 2592 trees and logarithmic biomass equations were developed using the diameter at breast height (cm) and total height (m) of the trees. Wood density increased with the age of the crop only at Paysandú. On average, wood density increased from 0.405 g cm -3 at age 18 months to 0.497 g cm -3 at ag... Presentar Todo |
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Palabras claves : |
BIOMASS; EUCALYPTS; INTENSIVE SILVIVULTURE; SHORT ROTATION; WOOD DENSITY. |
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Thesagro : |
BIOMASA; URUGUAY. |
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Asunto categoría : |
K10 Producción forestal |
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Marc : |
LEADER 03301naa a2200265 a 4500
001 1059486
005 2019-09-23
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.foreco.2019.02.007$2DOI
100 1 $aRESQUÍN, F.
245 $aInfluence of contrasting stocking densities on the dynamics of above-ground biomass and wood density of Eucalyptus benthamii, Eucalyptus dunnii, and Eucalyptus grandis for bioenergy in Uruguay.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 25 September 2018; Received in revised form 31 January 2019; Accepted 4 February 2019.
520 $aShort-rotation plantations have been suggested to develop renewable energies in South America. Our study evaluated the biomass production of Eucalyptus benthamii, E. dunnii and E. grandis plantations at stocking densities of 2220, 3330, 4440 and 6660 trees ha-1 in the North (Tacuarembó) and West (Paysandú) of Uruguay, over a 76-month period. The species survival was not related to planting density, and the highest mortality rates occurred at Tacuarembó. The effects of tree competition were more evident for E. grandis. At Tacuarembó site, the average survival of the species were: 57, 57 and 46% at age 76 months for E. benthamii, E. dunnii and E. grandis, respectively. Survival rates were similar for the different planting densities, with ranges between 50 and 57%. At Paysandú site, the average survival of the species were: 83, 86 and 75% for E. benthamii, E. dunnii and E. grandis, respectively, with a range from 78 to 84% for different planting densities. Weather conditions (temperature and rainfall) were similar at both sites throughout the evaluation period. Wood density and individual and total biomass weight (kg tree-1 and Mg ha-1,respectively) were evaluated sampling destructively 2592 trees and logarithmic biomass equations were developed using the diameter at breast height (cm) and total height (m) of the trees. Wood density increased with the age of the crop only at Paysandú. On average, wood density increased from 0.405 g cm -3 at age 18 months to 0.497 g cm -3 at age 76 months. The highest wood density was observed at 76 months on E. benthamii (0.413 and 0.431 g cm -3) and E. dunnii (0.496 and 0.539 g cm -3) at Tacuarembó and Paysandú, respectively. Age had no effect on the wood density at Tacuarembó. The stem biomass showed an inversely-proportional relationship with the planting density. Individual stem biomass was higher for E. grandis (81 and 74 kg tree -1 on average at Tacuarembó and Paysandú, respectively) than for the other species. The highest biomass per hectare was achieved for the highest planting density, specifically for E. dunnii (193 Mg ha -1) and E. grandis (203 Mg ha -1) at Paysandú and for E. dunnii (157 Mg ha -1) at Tacuarembó. At both locations, the effects of contrasting planting densities on biomass production increased throughout the rotation.
650 $aBIOMASA
650 $aURUGUAY
653 $aBIOMASS
653 $aEUCALYPTS
653 $aINTENSIVE SILVIVULTURE
653 $aSHORT ROTATION
653 $aWOOD DENSITY
700 1 $aNAVARRO-CERRILLO, R.M.
700 1 $aCARRASCO-LETELIER, L.
700 1 $aRACHID, C.
773 $tForest Ecology and Management, 2019$gv. 438, p. 63-74.
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