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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
02/05/2025 |
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Actualizado : |
02/05/2025 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
ARRUABARRENA, A.; VIDAL, S. |
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Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-2781-3613; SABINA VIDAL, Laboratorio de Biología Molecular Vegetal, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Agrobacterium-mediated transformation for gene editing tomato elite breeding lines. |
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Complemento del título : |
Protocol. Chapter 12. |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
In: Stange Klein, C. (eds). Agrobacterium. Methods in Molecular Biology, vol 2911. Humana, New York, NY. pp. 121-131. https://doi.org/10.1007/978-1-0716-4450-8_12 |
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Serie : |
Springer Protocols (Methods in Molecular Biology, 2911) |
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ISBN : |
978-1-0716-4449-2; e-ISBN: 978-1-0716-4450-8 |
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ISSN : |
1064-3745 |
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DOI : |
10.1007/978-1-0716-4450-8_12 |
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Idioma : |
Inglés |
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Notas : |
Chapter history: Published 28 March 2025. -- Correspondence: Arruabarrena, A.; Instituto Nacional de Investigación Agropecuaria, Estación Experimental INIA Salto Grande, Salto, Uruguay; email:aarruabarrena@inia.org.uy -- Funding: The authors thank the INIA Tomato Breeding Program for providing elite genotypes for in vitro regeneration and transformation adjustments, and the INIA (L1 HO_20_0_00) and CSIC (CSIC I+D 1492, UdelaR) projects for funding. -- Publisher: Humana Press Inc. |
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Contenido : |
ABSTRACT.- Genome editing success in crop species is largely dependent on the availability of highly efficient plant transformation protocols. Tomato (Solanum lycopersicum) was the first dicotyledonous crop to be successfully mutagenized using CRISPR-Cas9. Despite many efforts, no standardized, simple protocol is available for non-model tomato genotypes. With the increasing availability of gene editing tools, the transformation of elite tomato breeding lines has gained importance because mutant variants can be easily incorporated into breeding programs. This chapter describes a protocol for transforming and gene editing in elite tomato breeding lines, reaching 3.6% transformation efficiency. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025. |
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Palabras claves : |
Agrobacterium tumefaciens; CRISPR-Cas9; Hypocotyl; SISTEMA VEGETAL INTENSIVO - INIA; Solanum lycopersicum. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02169naa a2200241 a 4500
001 1065176
005 2025-05-02
008 2025 bl uuuu u00u1 u #d
022 $a1064-3745
024 7 $a10.1007/978-1-0716-4450-8_12$2DOI
100 1 $aARRUABARRENA, A.
245 $aAgrobacterium-mediated transformation for gene editing tomato elite breeding lines.$h[electronic resource]
260 $c2025
490 $aSpringer Protocols (Methods in Molecular Biology, 2911)
500 $aChapter history: Published 28 March 2025. -- Correspondence: Arruabarrena, A.; Instituto Nacional de Investigación Agropecuaria, Estación Experimental INIA Salto Grande, Salto, Uruguay; email:aarruabarrena@inia.org.uy -- Funding: The authors thank the INIA Tomato Breeding Program for providing elite genotypes for in vitro regeneration and transformation adjustments, and the INIA (L1 HO_20_0_00) and CSIC (CSIC I+D 1492, UdelaR) projects for funding. -- Publisher: Humana Press Inc.
520 $aABSTRACT.- Genome editing success in crop species is largely dependent on the availability of highly efficient plant transformation protocols. Tomato (Solanum lycopersicum) was the first dicotyledonous crop to be successfully mutagenized using CRISPR-Cas9. Despite many efforts, no standardized, simple protocol is available for non-model tomato genotypes. With the increasing availability of gene editing tools, the transformation of elite tomato breeding lines has gained importance because mutant variants can be easily incorporated into breeding programs. This chapter describes a protocol for transforming and gene editing in elite tomato breeding lines, reaching 3.6% transformation efficiency. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
653 $aAgrobacterium tumefaciens
653 $aCRISPR-Cas9
653 $aHypocotyl
653 $aSISTEMA VEGETAL INTENSIVO - INIA
653 $aSolanum lycopersicum
700 1 $aVIDAL, S.
773 $tIn: Stange Klein, C. (eds). Agrobacterium. Methods in Molecular Biology, vol 2911. Humana, New York, NY. pp. 121-131. https://doi.org/10.1007/978-1-0716-4450-8_12
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| 2. |  | RAMOS, Z.; GARRICK , D. J.; BLAIR, H. T.; VERA, B.; CIAPPESONI, G.; KENYON, P. R. Genomic regions associated with wool, growth and reproduction traits in Uruguayan Merino Sheep. Genes, 2023, volume 14, issue 1, article 167. OPEN ACCESS. doi: https://doi.org/10.3390/genes14010167 Article history: Received 13 December 2022; Revised 29 December 2022; Accepted 4 January 2023; Published 7 January 2023. -- Correspondence author: Zully Ramos, email: z.ramosalvez@massey.ac.nz -- Academic Editor: Qiuyue Liu -- This...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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| 3. | | NAVAJAS, E.; GARRICK, D.J.; PLEASANTS, A.B.; MORRIS, C.A. Superiority of a biochemically-based approach for the detection of a quantitative trait locus affecting ultimate pH. Session 11. Growth and meat quality Communication No. 11-16. In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 7., Montpellier, France, August 19-23, 2002, p. 11-16.| Tipo: Trabajos en Congresos/Conferencias |
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| 4. | | RAMOS, Z.; GARRICK, D.J.; BLAIR, H.T.; DE BARBIERI, I.; CIAPPESONI, G.; MONTOSSI, F.; KENYON, P.R. Genetic and phenotypic relationships between ewe reproductive performance and wool and growth traits in Uruguayan Ultrafine Merino sheep. Journal of Animal Science, 2023, Volume 101, skad071. OPEN ACCESS. https://doi.org/10.1093/jas/skad071 Article history: Received 13 July 2022; Accepted 06 March 2023; Published 07 March 2023; Corrected and typeset 14 April 2023. -- Corresponding author: z.ramosalvez@massey.ac.nz -- Issue Section: Animal Genetics and Genomics...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 5. | | RAMOS, Z.; GARRICK, D. J.; BLAIR, H. T.; DE BARBIERI, I.; CIAPPESONI, G.; MONTOSSI, F.; KENYON, P. R. Genetic trends for production and reproduction traits in ultrafine Merino sheep of Uruguay. Original article. Journal of Animal Breeding and Genetics, 2025. Early view. https://doi.org/10.1111/jbg.12937 -- OPEN ACCESS. Article history: Received 8 June 2024, Revised 22 February 2025, Accepted 11 March 2025, First published 21 March 2025. -- Corresponding: Kenyon, P.R.; School of Agriculture and Environment, Massey University, Palmerston North, New...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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