| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
10/09/2025 |
|
Actualizado : |
10/09/2025 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
FERNANDES, P. B. B.; FONSECA, M. E. N.; GONZÁLEZ-ARCOS, M.; BOITEUX, L. S.; ARAGÃO, F. J. L. |
|
Afiliación : |
PEDRO B. B. FERNANDES, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil; Departamento de Biologia Celular, Universidade de Brasília, Campus Universitário, DF, Brasília, 70910-900, Brazil; MARIA E. N. FONSECA, Embrapa Hortaliças, Rodovia BR-060, Km 09, Fazenda Tamanduá, DF, Brasília, 70275-970, Brazil; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONARDO S. BOITEUX, Embrapa Hortaliças, Rodovia BR-060, Km 09, Fazenda Tamanduá, DF, Brasília, 70275-970, Brazil; FRANCISCO J. L. ARAGÃO, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil; Departamento de Biologia Celular, Universidade de Brasília, Campus Universitário, DF, Brasília, 70910-900, Brazil. |
|
Título : |
Remodeling aboveground tomato plant architecture via CRISPR/Cas9-mediated editing of a single Tiller Angle Control 1-like gene. |
|
Fecha de publicación : |
2025 |
|
Fuente / Imprenta : |
aBIOTECH, 2025. [In Press]. https://doi.org/10.1007/s42994-025-00220-9 -- |
|
ISSN : |
2096-6326. |
|
DOI : |
10.1007/s42994-025-00220-9 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 27 March 2025, Accepted 19 May 2025. -- Correspondence: F.J.L. Aragão, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil, Email: francisco.aragao@embrapa.br -- Funding: The authors gratefully acknowledge the financial support of Conselho Nacional de Desenvolvimento Cientıíico e Tecnológico (CNPq), Brazil (Grant No. 304001/2023-6). Pedro B.B. Fernandes was supported by a fellowship from CNPq (Grant No. 140365/2023-0). This research was also supported by grants, scholarships, and fellowships from Embrapa (MENF, LSB, and FJLA), FAP-DF (MENF), and CNPq (MENF, LSB, and FJLA). |
|
Contenido : |
ABSTRACT.- Plant architecture is a major factor affecting crop management and yield. The erect leaf phenotype is a key trait for improving light capture, reducing water loss, optimizing space utilization, and facilitating the chemical and biological control of arthropods and pathogens, especially those infesting/infecting abaxial leaf surfaces. This phenotype has been associated with Tiller Angle Control 1 (TAC1)-like genes across many herbaceous and tree species. Our previous genomic and genetic analyses of the erect leaf phenotype in tomato (Solanum lycopersicum) indicated that this trait is controlled by a semi-dominant locus, Erl, on chromosome 10. We discovered that this phenotype was in tight linkage with a candidate loss-of-function mutation in Solyc10g009320, an ortholog of TAC1-like genes. Therefore, editing this gene might confirm its function and enable the fine-tuned manipulation of aboveground tomato plant architecture. Here, we utilized a CRISPR/Cas9 genome editing system to confirm the complete genetic association of the erect leaf phenotype in tomato by knocking out Solyc10g009320 in tomato cultivar 'Micro-Tom'. In addition, we analyzed the effects of editing this gene on the overall plant phenotype as well as physiological and agronomic performance. Editing Solyc10g009320 alleles in tomato lays the foundation for the large-scale generation of superior genotypes, paving the way for the development of elite cultivars with an erect leaf phenotype.
© Agricultural Information Institute, Chinese Academy of Agricultural Sciences 2025. MenosABSTRACT.- Plant architecture is a major factor affecting crop management and yield. The erect leaf phenotype is a key trait for improving light capture, reducing water loss, optimizing space utilization, and facilitating the chemical and biological control of arthropods and pathogens, especially those infesting/infecting abaxial leaf surfaces. This phenotype has been associated with Tiller Angle Control 1 (TAC1)-like genes across many herbaceous and tree species. Our previous genomic and genetic analyses of the erect leaf phenotype in tomato (Solanum lycopersicum) indicated that this trait is controlled by a semi-dominant locus, Erl, on chromosome 10. We discovered that this phenotype was in tight linkage with a candidate loss-of-function mutation in Solyc10g009320, an ortholog of TAC1-like genes. Therefore, editing this gene might confirm its function and enable the fine-tuned manipulation of aboveground tomato plant architecture. Here, we utilized a CRISPR/Cas9 genome editing system to confirm the complete genetic association of the erect leaf phenotype in tomato by knocking out Solyc10g009320 in tomato cultivar 'Micro-Tom'. In addition, we analyzed the effects of editing this gene on the overall plant phenotype as well as physiological and agronomic performance. Editing Solyc10g009320 alleles in tomato lays the foundation for the large-scale generation of superior genotypes, paving the way for the development of elite cultivars with an erect leaf phenotype.
© Agricultur... Presentar Todo |
|
Palabras claves : |
Dense cultivation; Erect leaf phenotype; Gene editing; Genetically modified plants; INIA SALTO GRANDE; SISTEMA VEGETAL INTENSIVO - INIA; Tomato; Transgenic plant. |
|
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
|
Marc : |
LEADER 03189naa a2200301 a 4500
001 1065357
005 2025-09-10
008 2025 bl uuuu u00u1 u #d
022 $a2096-6326.
024 7 $a10.1007/s42994-025-00220-9$2DOI
100 1 $aFERNANDES, P. B. B.
245 $aRemodeling aboveground tomato plant architecture via CRISPR/Cas9-mediated editing of a single Tiller Angle Control 1-like gene.$h[electronic resource]
260 $c2025
500 $aArticle history: Received 27 March 2025, Accepted 19 May 2025. -- Correspondence: F.J.L. Aragão, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil, Email: francisco.aragao@embrapa.br -- Funding: The authors gratefully acknowledge the financial support of Conselho Nacional de Desenvolvimento Cientıíico e Tecnológico (CNPq), Brazil (Grant No. 304001/2023-6). Pedro B.B. Fernandes was supported by a fellowship from CNPq (Grant No. 140365/2023-0). This research was also supported by grants, scholarships, and fellowships from Embrapa (MENF, LSB, and FJLA), FAP-DF (MENF), and CNPq (MENF, LSB, and FJLA).
520 $aABSTRACT.- Plant architecture is a major factor affecting crop management and yield. The erect leaf phenotype is a key trait for improving light capture, reducing water loss, optimizing space utilization, and facilitating the chemical and biological control of arthropods and pathogens, especially those infesting/infecting abaxial leaf surfaces. This phenotype has been associated with Tiller Angle Control 1 (TAC1)-like genes across many herbaceous and tree species. Our previous genomic and genetic analyses of the erect leaf phenotype in tomato (Solanum lycopersicum) indicated that this trait is controlled by a semi-dominant locus, Erl, on chromosome 10. We discovered that this phenotype was in tight linkage with a candidate loss-of-function mutation in Solyc10g009320, an ortholog of TAC1-like genes. Therefore, editing this gene might confirm its function and enable the fine-tuned manipulation of aboveground tomato plant architecture. Here, we utilized a CRISPR/Cas9 genome editing system to confirm the complete genetic association of the erect leaf phenotype in tomato by knocking out Solyc10g009320 in tomato cultivar 'Micro-Tom'. In addition, we analyzed the effects of editing this gene on the overall plant phenotype as well as physiological and agronomic performance. Editing Solyc10g009320 alleles in tomato lays the foundation for the large-scale generation of superior genotypes, paving the way for the development of elite cultivars with an erect leaf phenotype. © Agricultural Information Institute, Chinese Academy of Agricultural Sciences 2025.
653 $aDense cultivation
653 $aErect leaf phenotype
653 $aGene editing
653 $aGenetically modified plants
653 $aINIA SALTO GRANDE
653 $aSISTEMA VEGETAL INTENSIVO - INIA
653 $aTomato
653 $aTransgenic plant
700 1 $aFONSECA, M. E. N.
700 1 $aGONZÁLEZ-ARCOS, M.
700 1 $aBOITEUX, L. S.
700 1 $aARAGÃO, F. J. L.
773 $taBIOTECH, 2025. [In Press]. https://doi.org/10.1007/s42994-025-00220-9 --
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
|
Fecha actual : |
21/12/2020 |
|
Actualizado : |
21/12/2020 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
FERRINHO, A.M.; PERIPOLLI, E.; BANCHERO, G.; PEREIRA, A.S.C.; BRITO, G.; LA MANNA, A.; FERNANDEZ, E.; MONTOSSI, F.; KLUSKA, S.; MUELLER, L.F.; BERCHIELLI, T.T.; BALDI, F. |
|
Afiliación : |
ADRIELLE MATIAS FERRINHO, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil; Instituto Nacional de Ciência e Tecnologia-Ciencia Animal, Viçosa, MG, Brazil; ELISA PERIPOLLI, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANGÉLICA SIMONE CRAVO PEREIRA, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil; GUSTAVO WALTER BRITO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO LA MANNA ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE GENARO FERNANDEZ RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SABRINA KLUSKA, Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil; LENISE FREITAS MUELLER, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil; TELMA TERESINHA BERCHIELLI, Dpto Zootecnia, Fac. Ciências Agrárias e Veterinárias, Univ. Estadual Paulista, SP, Brazil; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brasília, DF, Brazil; Instituto Nacional de Ciência e Tecnologia-Ciencia Animal, Viçosa, MG, Brazil; FERNANDO SEBASTIAN BALDI REY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Effect of growth path on carcass and meat-quality traits of Hereford steers finished on pasture or in feedlot. |
|
Fecha de publicación : |
2020 |
|
Fuente / Imprenta : |
Animal Production Science, 2020, Volume 60, Issue 2, Pages 323-332. Doi: https://doi.org/10.1071/AN18075 |
|
ISSN : |
1836-0939 |
|
DOI : |
10.1071/AN18075 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 30 January 2018 / Accepted 14 May 2019 / Published online 4 December 2019.
Funding information:
This work was supported by Sao Paulo Research Foundation (FAPESP grant #2016/22022-4 and #2011/21241-0), Brazil, and the National Institute of Agricultural research (INIA) of Uruguay. |
|
Contenido : |
ABSTRACT.
The objective of this study was to evaluate the effect of nutritional management during rearing and finishing phases on beef fatty acid composition, and carcass and beef quality traits of Hereford cattle. The study used 240 castrated male calves weaned at 8 months of age, and with an average weight of 170 ± 17 kg. After weaning, the experiment was divided in to three phases in a 4 × 2 factorial design: a 93-day winter period with four treatment groups (on pasture or in feedlot and at high or low feeding levels); a 196-day compensatory-growth phase on pasture; and a finishing phase either on pasture or in feedlot. Animals were slaughtered when each group attained a mean liveweight of 500 kg. The winter growth × finishing management interaction significantly affected hot carcass weight (P = 0.0029). There was no differences observed for feedlot-finished steers, but for pasture-finished steers, those pasture-reared had higher hot carcass weight (kg) than those feedlot-reared (low pasture 256.30 ± 1.60, high pasture 253.72 ± 1.60, low feedlot 249.85 ± 1.66, high feedlot 247.60 ± 1.62). Feedlot-finished steers showed higher (P < 0.05) mean values than pasture-finished steers for ribeye area (55.61 ± 0.69 cm2 vs 53.18 cm2), backfat thickness (8.62 ± 0.32 mm vs 6.21 mm), marbling score (237.97 ± 13.06 vs 171.70) and final pH (5.53 ± 0.02 vs 5.48). Additionally, feedlot-finished steers raised in feedlot during the winter-growth period displayed the heaviest hindquarter cuts. Meat from pasture-finished steers had lower (P < 0.05) shear-force values than from feedlot-finished cattle (2.95 ± 0.18 vs 3.66 ± 0.17 kg), and when reared on either high or low pasture during winter-growth, they showed the highest (P < 0.05) conjugated linoleic acid (cis-9, trans-11) and n-3 polyunsaturated fatty acid concentrations. In conclusion, growing and finishing cattle on pasture improved the carcass yield of retail cuts because of low fat concentration, and improved the nutritional and health value of the beef fatty acid profile.
© 2020 CSIRO. MenosABSTRACT.
The objective of this study was to evaluate the effect of nutritional management during rearing and finishing phases on beef fatty acid composition, and carcass and beef quality traits of Hereford cattle. The study used 240 castrated male calves weaned at 8 months of age, and with an average weight of 170 ± 17 kg. After weaning, the experiment was divided in to three phases in a 4 × 2 factorial design: a 93-day winter period with four treatment groups (on pasture or in feedlot and at high or low feeding levels); a 196-day compensatory-growth phase on pasture; and a finishing phase either on pasture or in feedlot. Animals were slaughtered when each group attained a mean liveweight of 500 kg. The winter growth × finishing management interaction significantly affected hot carcass weight (P = 0.0029). There was no differences observed for feedlot-finished steers, but for pasture-finished steers, those pasture-reared had higher hot carcass weight (kg) than those feedlot-reared (low pasture 256.30 ± 1.60, high pasture 253.72 ± 1.60, low feedlot 249.85 ± 1.66, high feedlot 247.60 ± 1.62). Feedlot-finished steers showed higher (P < 0.05) mean values than pasture-finished steers for ribeye area (55.61 ± 0.69 cm2 vs 53.18 cm2), backfat thickness (8.62 ± 0.32 mm vs 6.21 mm), marbling score (237.97 ± 13.06 vs 171.70) and final pH (5.53 ± 0.02 vs 5.48). Additionally, feedlot-finished steers raised in feedlot during the winter-growth period displayed the heaviest hindquarter cut... Presentar Todo |
|
Palabras claves : |
Carry-over effects; Fatty-acid profile; Finishing system; Growth-out. |
|
Asunto categoría : |
L01 Ganadería |
|
Marc : |
LEADER 03376naa a2200337 a 4500
001 1060696
005 2020-12-21
008 2020 bl uuuu u00u1 u #d
022 $a1836-0939
024 7 $a10.1071/AN18075$2DOI
100 1 $aFERRINHO, A.M.
245 $aEffect of growth path on carcass and meat-quality traits of Hereford steers finished on pasture or in feedlot.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 30 January 2018 / Accepted 14 May 2019 / Published online 4 December 2019. Funding information: This work was supported by Sao Paulo Research Foundation (FAPESP grant #2016/22022-4 and #2011/21241-0), Brazil, and the National Institute of Agricultural research (INIA) of Uruguay.
520 $aABSTRACT. The objective of this study was to evaluate the effect of nutritional management during rearing and finishing phases on beef fatty acid composition, and carcass and beef quality traits of Hereford cattle. The study used 240 castrated male calves weaned at 8 months of age, and with an average weight of 170 ± 17 kg. After weaning, the experiment was divided in to three phases in a 4 × 2 factorial design: a 93-day winter period with four treatment groups (on pasture or in feedlot and at high or low feeding levels); a 196-day compensatory-growth phase on pasture; and a finishing phase either on pasture or in feedlot. Animals were slaughtered when each group attained a mean liveweight of 500 kg. The winter growth × finishing management interaction significantly affected hot carcass weight (P = 0.0029). There was no differences observed for feedlot-finished steers, but for pasture-finished steers, those pasture-reared had higher hot carcass weight (kg) than those feedlot-reared (low pasture 256.30 ± 1.60, high pasture 253.72 ± 1.60, low feedlot 249.85 ± 1.66, high feedlot 247.60 ± 1.62). Feedlot-finished steers showed higher (P < 0.05) mean values than pasture-finished steers for ribeye area (55.61 ± 0.69 cm2 vs 53.18 cm2), backfat thickness (8.62 ± 0.32 mm vs 6.21 mm), marbling score (237.97 ± 13.06 vs 171.70) and final pH (5.53 ± 0.02 vs 5.48). Additionally, feedlot-finished steers raised in feedlot during the winter-growth period displayed the heaviest hindquarter cuts. Meat from pasture-finished steers had lower (P < 0.05) shear-force values than from feedlot-finished cattle (2.95 ± 0.18 vs 3.66 ± 0.17 kg), and when reared on either high or low pasture during winter-growth, they showed the highest (P < 0.05) conjugated linoleic acid (cis-9, trans-11) and n-3 polyunsaturated fatty acid concentrations. In conclusion, growing and finishing cattle on pasture improved the carcass yield of retail cuts because of low fat concentration, and improved the nutritional and health value of the beef fatty acid profile. © 2020 CSIRO.
653 $aCarry-over effects
653 $aFatty-acid profile
653 $aFinishing system
653 $aGrowth-out
700 1 $aPERIPOLLI, E.
700 1 $aBANCHERO, G.
700 1 $aPEREIRA, A.S.C.
700 1 $aBRITO, G.
700 1 $aLA MANNA, A.
700 1 $aFERNANDEZ, E.
700 1 $aMONTOSSI, F.
700 1 $aKLUSKA, S.
700 1 $aMUELLER, L.F.
700 1 $aBERCHIELLI, T.T.
700 1 $aBALDI, F.
773 $tAnimal Production Science, 2020, Volume 60, Issue 2, Pages 323-332. Doi: https://doi.org/10.1071/AN18075
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
