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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
09/04/2025 |
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Actualizado : |
09/04/2025 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PREISEGGER, L.; FLECHA, J. C.; GHILINI, F.; ESPIN-SÁNCHEZ, D.; PRIETO, E.; OBERTI, H.; ABREO, E.; HUARTE-BONNET, C.; PEDRINI, N.; MANNINO, M. C. |
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Afiliación : |
LAUTARO PREISEGGER, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Calles 60 y 120, La Plata 1900, Argentina; JUAN CRUZ FLECHA, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Calles 60 y 120, La Plata 1900, Argentina; FIORELLA GHILINI, Instituto de Investigaciones Fisicoquìmicas Teóricas y Aplicadas (INIFTA), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Diagonal 113 y 64 S/N, La Plata 1900, Argentina; DAYSI ESPIN-SÁNCHEZ, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Calles 60 y 120, La Plata 1900, Argentina; EDUARDO PRIETO, Instituto de Investigaciones Fisicoquìmicas Teóricas y Aplicadas (INIFTA), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Diagonal 113 y 64 S/N, La Plata 1900, Argentina; Instit; HÉCTOR OBERTI RVAROLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Departamento de Biotecnología, Universidad ORT Uruguay, Montevideo, 10129, Uruguay;ORCID: https://orcid.org/0000-0002-4956-9914; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0001-8517-0823; CARLA HUARTE-BONNET, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Calles 60 y 120, La Plata 1900, Argentina; NICOLÁS PEDRINI, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Calles 60 y 120, La Plata 1900, Argentina; MARIA CONSTANZA MANNINO, Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata (UNLP), Calles 60 y 120, La Plata 1900, Argentina. |
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Título : |
Visualizing oral infection dynamics of Beauveria bassiana in the gut of Tribolium castaneum. |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
Journal of Fungi, February 2025, Volume 11, Issue 2, 101. https://doi.org/10.3390/jof11020101 -- OPEN ACCESS. |
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ISSN : |
2309-608X |
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DOI : |
10.3390/jof11020101 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submission received 2 January 2025, Revised 24 January 2025, Accepted 26 January 2025, Published 28 January 2025. -- Academic editor: Chengshu Wang. -- Funding: This research and the APC were funded by Agencia Nacional de Promoción Científica y Tecnológica, grant number PICT 2020 serie A 02932. --
Supplementary materials at: https://www.mdpi.com/article/10.3390/jof11020101/s1 -- This article belongs to the Special Issue Fungi and Insect Interactions: Pathogenicity, Immune Defenses and Biocontrol (https://www.mdpi.com/journal/jof/special_issues/265WR9CQ1W ) -- Document type: Article, Gold Open Access. -- Publisher: Multidisciplinary Digital Publishing Institute (MDPI). -- License: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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Contenido : |
ABSTRACT.- The ability of entomopathogenic fungi, such as Beauveria bassiana, to infect insects by penetrating their cuticle is well documented. However, some insects have evolved mechanisms to combat fungal infections. The red flour beetle (Tribolium castaneum), a major pest causing significant economic losses in stored product environments globally, embeds antifungal compounds within its cuticle as a protective barrier. Previous reports have addressed the contributions of non-cuticular infection routes, noting an increase in mortality in beetles fed with conidia. In this study, we further explore the progression and dynamics of oral exposure in the gut of T. castaneum after feeding with an encapsulated B. bassiana conidia formulation. First, we characterized the formulation surface using atomic force microscopy, observing no significant topological differences between capsules containing and not containing conidia. Confocal microscopy confirmed uniform conidia distribution within the hydrogel matrix. Then, larvae and adult insects fed with the conidia-encapsulated formulation exhibited B. bassiana distributed throughout the alimentary canal, with a higher presence of conidia before the pyloric chamber. More conidia were found in the larval midgut and hindgut compared to adults, but no germinated conidia were observed in the epithelium. These results suggest that the presence of conidia obstructs the gut, impairing the insect's ability to ingest, process, and absorb nutrients. This disruption may weaken the host, increasing its susceptibility to infections and, ultimately, leading to death. By providing the first direct observation of fungal conidia within the alimentary canal of T. castaneum, this study highlights a novel aspect of fungal-host interaction and opens new avenues for advancing fungal-based pest control strategies by exploiting stage-specific vulnerabilities. © 2025 by the authors. MenosABSTRACT.- The ability of entomopathogenic fungi, such as Beauveria bassiana, to infect insects by penetrating their cuticle is well documented. However, some insects have evolved mechanisms to combat fungal infections. The red flour beetle (Tribolium castaneum), a major pest causing significant economic losses in stored product environments globally, embeds antifungal compounds within its cuticle as a protective barrier. Previous reports have addressed the contributions of non-cuticular infection routes, noting an increase in mortality in beetles fed with conidia. In this study, we further explore the progression and dynamics of oral exposure in the gut of T. castaneum after feeding with an encapsulated B. bassiana conidia formulation. First, we characterized the formulation surface using atomic force microscopy, observing no significant topological differences between capsules containing and not containing conidia. Confocal microscopy confirmed uniform conidia distribution within the hydrogel matrix. Then, larvae and adult insects fed with the conidia-encapsulated formulation exhibited B. bassiana distributed throughout the alimentary canal, with a higher presence of conidia before the pyloric chamber. More conidia were found in the larval midgut and hindgut compared to adults, but no germinated conidia were observed in the epithelium. These results suggest that the presence of conidia obstructs the gut, impairing the insect's ability to ingest, process, and absorb nutrien... Presentar Todo |
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Palabras claves : |
AFM; Alginate; ÁREA DE RECURSOS NATURALES, PRODUCCIÓN Y AMBIENTE - INIA; Coleoptera; EGFP; Entomopathogenic fungi; Insect gut; Oral infection; PLATAFORMA DE BIOINSUMOS - INIA. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://www.mdpi.com/2309-608X/11/2/101/pdf
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Marc : |
LEADER 03934naa a2200373 a 4500
001 1065162
005 2025-04-09
008 2025 bl uuuu u00u1 u #d
022 $a2309-608X
024 7 $a10.3390/jof11020101$2DOI
100 1 $aPREISEGGER, L.
245 $aVisualizing oral infection dynamics of Beauveria bassiana in the gut of Tribolium castaneum.$h[electronic resource]
260 $c2025
500 $aArticle history: Submission received 2 January 2025, Revised 24 January 2025, Accepted 26 January 2025, Published 28 January 2025. -- Academic editor: Chengshu Wang. -- Funding: This research and the APC were funded by Agencia Nacional de Promoción Científica y Tecnológica, grant number PICT 2020 serie A 02932. -- Supplementary materials at: https://www.mdpi.com/article/10.3390/jof11020101/s1 -- This article belongs to the Special Issue Fungi and Insect Interactions: Pathogenicity, Immune Defenses and Biocontrol (https://www.mdpi.com/journal/jof/special_issues/265WR9CQ1W ) -- Document type: Article, Gold Open Access. -- Publisher: Multidisciplinary Digital Publishing Institute (MDPI). -- License: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
520 $aABSTRACT.- The ability of entomopathogenic fungi, such as Beauveria bassiana, to infect insects by penetrating their cuticle is well documented. However, some insects have evolved mechanisms to combat fungal infections. The red flour beetle (Tribolium castaneum), a major pest causing significant economic losses in stored product environments globally, embeds antifungal compounds within its cuticle as a protective barrier. Previous reports have addressed the contributions of non-cuticular infection routes, noting an increase in mortality in beetles fed with conidia. In this study, we further explore the progression and dynamics of oral exposure in the gut of T. castaneum after feeding with an encapsulated B. bassiana conidia formulation. First, we characterized the formulation surface using atomic force microscopy, observing no significant topological differences between capsules containing and not containing conidia. Confocal microscopy confirmed uniform conidia distribution within the hydrogel matrix. Then, larvae and adult insects fed with the conidia-encapsulated formulation exhibited B. bassiana distributed throughout the alimentary canal, with a higher presence of conidia before the pyloric chamber. More conidia were found in the larval midgut and hindgut compared to adults, but no germinated conidia were observed in the epithelium. These results suggest that the presence of conidia obstructs the gut, impairing the insect's ability to ingest, process, and absorb nutrients. This disruption may weaken the host, increasing its susceptibility to infections and, ultimately, leading to death. By providing the first direct observation of fungal conidia within the alimentary canal of T. castaneum, this study highlights a novel aspect of fungal-host interaction and opens new avenues for advancing fungal-based pest control strategies by exploiting stage-specific vulnerabilities. © 2025 by the authors.
653 $aAFM
653 $aAlginate
653 $aÁREA DE RECURSOS NATURALES, PRODUCCIÓN Y AMBIENTE - INIA
653 $aColeoptera
653 $aEGFP
653 $aEntomopathogenic fungi
653 $aInsect gut
653 $aOral infection
653 $aPLATAFORMA DE BIOINSUMOS - INIA
700 1 $aFLECHA, J. C.
700 1 $aGHILINI, F.
700 1 $aESPIN-SÁNCHEZ, D.
700 1 $aPRIETO, E.
700 1 $aOBERTI, H.
700 1 $aABREO, E.
700 1 $aHUARTE-BONNET, C.
700 1 $aPEDRINI, N.
700 1 $aMANNINO, M. C.
773 $tJournal of Fungi, February 2025, Volume 11, Issue 2, 101. https://doi.org/10.3390/jof11020101 -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 7 | |
| 1. |  | ABREO, E.; GIROTTI, J.; MIJAILOVSKY, S.; JUÁREZ, P.; ALTIER, N.; ZERBINO, M.; PEDRINI, N. Determinación de los hidrocarburos de la epicutícula de insectos plaga como herramienta para la selección y producción masiva de agentes microbianos de control biológico. In: JORNADA NACIONAL DE FITOPATOLOGÍA, 3; JORNADA NACIONAL DE PROTECCIÓN VEGETAL, 1., 3 SETIEMBRE 2015, MONTEVIDEO, URUGUAY. Libro de Resúmenes. Montevideo (Uruguay) : SUFIT, 2015. p. 43| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 2. | | PREISEGGER, L.; FLECHA, J. C.; GHILINI, F.; ESPIN-SÁNCHEZ, D.; PRIETO, E.; OBERTI, H.; ABREO, E.; HUARTE-BONNET, C.; PEDRINI, N.; MANNINO, M. C. Visualizing oral infection dynamics of Beauveria bassiana in the gut of Tribolium castaneum. Journal of Fungi, February 2025, Volume 11, Issue 2, 101. https://doi.org/10.3390/jof11020101 -- OPEN ACCESS. Article history: Submission received 2 January 2025, Revised 24 January 2025, Accepted 26 January 2025, Published 28 January 2025. -- Academic editor: Chengshu Wang. -- Funding: This research and the APC were funded by Agencia Nacional...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 3. | | SESSA, L.; CALDERÓN-FERNÁNDEZ, G.M.; ABREO, E.; ALTIER, N.; MIJAILOVSKY, S.J.; GIROTTI, J.R.; PEDRINI, N. Epicuticular hydrocarbons of the redbanded stink bug Piezodorus guildinii (Heteroptera: Pentatomidae): sexual dimorphism and alterations in insects collected in insecticide-treated soybean crops. Pest Management Science, 2021. Doi: https://doi.org/10.1002/ps.6528 Article history: Received 8 June 2021, Accepted article published, 24 June 2021.
This work was financed by Agencia Nacional de Investigación e Innovación (ANII) grant POS_NAC_2016_1_130245 and Instituto Nacional de Investigación...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 6. | | SESSA, L.; PEDRINI, N.; ALTIER, N.; ABREO, E. Conociendo al enemigo: Hidrocarburos epicuticulares de insectos plaga y su utilización para el biocontrol. O11. Módulo 3: Nuevas estrategias para potenciar el control biológico. In: Abreo, E.; Beyhaut, E.; Rivas, F. (Eds.). Simposio Microorganismos para la Agricultura, 2. [Resúmenes y Posters]. Canelones (UY): INIA, 2022. p.22. (Serie Actividades de Difusión; 801). Financiamiento: Beca ANII, INIA, Beca CAP.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 7. | | SESSA, L.; PEDRINI, N.; ALTIER, N.; ABREO, E. Hidrocarburos epicuticulares de Piezodorus guildinii: efecto de los insecticidas y uso de n-pentadecano para aumentar la virulencia de cepas de Beauveria bassiana. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. p. 70 Fianciamiento: INIA-ANII-PICT.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Treinta y Tres. |
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| Registros recuperados : 7 | |
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