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| 1. |  | LIEBIG, M. A.; FRANZLUEBBERS, A. J.; ALVAREZ, C.; CHIESA, T. D.; LEWCZUK, N.; PIÑEIRO, G.; POSSE, G.; YAHDJIAN, L.; GRACE, P.; CABRAL, O. M. R.; MARTIN NETO, L.; RODRIGUES, R. DE A. R.; AMIRO, B.; ANGERS, D.; HAO, X.; OELBERMANN, M.; TENUTA, M.; MUNKHOLM, L. J.; REGINA, K.; CELLIER, P.; EHRHARDT, F.; RICHARD, G.; DECHOW, R.; AGUS, F.; WIDIARTA, N.; SPINK, J.; BERTI, A.; GRIGNANI, C.; MAZZONCINI, M.; ORSINI, R.; ROGGERO, P. P.; SEDDAIU, G.; TEI, F.; VENTRELLA, D.; VITALI, G.; KISHIMOTO-MO, A.; SHIRATO, Y.; SUDO, S.; SHIN, J.; SCHIPPER, L.; SAVÉ, R.; LEIFELD, J.; SPADAVECCHIA, L.; YELURIPATI, J.; DEL GROSSO, S.; RICE, C.; SAWCHIK, J. MAGGnet: an international network to foster mitigation of agricultural greenhouse gases. Carbon Management v. 7 (3-4): 243-248, 2016. OPEN ACCESS. Published online: 31 May 2016.
This work was authored as part of the Contributor's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105,...| Biblioteca(s): INIA Las Brujas. |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
08/07/2024 |
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Actualizado : |
08/07/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
NARANCIO, R.; ISENEGGER, D.; REYNO, R.; SPANGENBERG, G.; DALLA RIZZA, M. |
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Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-5192-2887; DANIEL ISENEGGER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, 5 Ring Road, Melbourne, 3083, Australia; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0001-9619-8477; GERMAN SPANGENBERG, La Trobe University, AgriBio, Centre for AgriBioscience, 5 Ring Road, Melbourne, 3083, Australia; Qingdao Agricultural University, 700 Changcheng Road, Qingdao, 266000, China; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0003-4065-0940. |
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Título : |
Tissue culture and high-efficiency transformation in an apomictic initial variety of Paspalum notatum Flüggé. |
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Fecha de publicación : |
2024 |
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Fuente / Imprenta : |
In Vitro Cellular and Developmental Biology - Plant, 2024. https://doi.org/10.1007/s11627-024-10434-z |
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ISSN : |
1054-5476 |
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DOI : |
10.1007/s11627-024-10434-z |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 January 2024, Accepted 15 April 2024, Published 19 June 2024. -- Correspondence: Narancio, R.; Instituto Nacional de Investigación Agropecuaria (INIA), Mejoramiento Genético y Biotecnología Vegetal. INIA Las Brujas, Ruta 48 Km 10, Canelones, Uruguay; email:rafael.narancio@agriculture.vic.gov.au -- |
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Contenido : |
ABSTRACT.- Paspalum notatum Flüggé is a subtropical grass native from the Americas and one of the main components of the subtropical South American grasslands. Although P. notatum exhibits a high forage productivity and persistence, some limitations are observed in the species, such as forage quality due to high lignin content. The development of efficient tissue culture and plant transformation protocols in cultivars for forage production are necessary to embrace novel genetic improvement through transgenesis and genome editing. Efficient regeneration and transformation systems for many plant species can be hampered by genotype dependency, which can only be determined through extensive laboratory testing and evaluation. In this study, various factors extracted from protocols have been evaluated to establish efficient somatic embryogenesis, plant regeneration, and genetic transformation in apomictic initial variety INIA Sepé. Optimal somatic embryogenesis and regeneration from explant sources, such as mature seeds and meristematic tissue derived from in vitro propagated tillers, ranged between 79 and 88%. Microprojectile bombardment of two plasmids encoding a constitutively regulated reporter gene and nptII selectable marker expression constructs showed an average transformation efficiency of 10.3% from meristematic tissue as an explant source. Furthermore, the constitutive promoters, 35S and ZmUbi, were tested in GFP transient expression assays to evaluate optimal cassettes for transgene(s) expression. Translation of a wheat codon-optimized Cas9 sequence using Cas9:2A:tuGFP fusion protein was also tested in protoplasts. The information generated on promoter activity and Cas9 translation, and the establishment of an efficient transformation system, provided useful tools for future work in genetic engineering and genome editing in P. notatum cv. INIA Sepé. © The Society for In Vitro Biology 2024. MenosABSTRACT.- Paspalum notatum Flüggé is a subtropical grass native from the Americas and one of the main components of the subtropical South American grasslands. Although P. notatum exhibits a high forage productivity and persistence, some limitations are observed in the species, such as forage quality due to high lignin content. The development of efficient tissue culture and plant transformation protocols in cultivars for forage production are necessary to embrace novel genetic improvement through transgenesis and genome editing. Efficient regeneration and transformation systems for many plant species can be hampered by genotype dependency, which can only be determined through extensive laboratory testing and evaluation. In this study, various factors extracted from protocols have been evaluated to establish efficient somatic embryogenesis, plant regeneration, and genetic transformation in apomictic initial variety INIA Sepé. Optimal somatic embryogenesis and regeneration from explant sources, such as mature seeds and meristematic tissue derived from in vitro propagated tillers, ranged between 79 and 88%. Microprojectile bombardment of two plasmids encoding a constitutively regulated reporter gene and nptII selectable marker expression constructs showed an average transformation efficiency of 10.3% from meristematic tissue as an explant source. Furthermore, the constitutive promoters, 35S and ZmUbi, were tested in GFP transient expression assays to evaluate optimal cassettes... Presentar Todo |
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Palabras claves : |
ÁREA DE PASTURAS Y FORRAJES - INIA; C4 grasses; Constitutive promoters; MEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA; Regeneration; Somatic embryogenesis; Transformation. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
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001 1064723
005 2024-07-08
008 2024 bl uuuu u00u1 u #d
022 $a1054-5476
024 7 $a10.1007/s11627-024-10434-z$2DOI
100 1 $aNARANCIO, R.
245 $aTissue culture and high-efficiency transformation in an apomictic initial variety of Paspalum notatum Flüggé.$h[electronic resource]
260 $c2024
500 $aArticle history: Received 15 January 2024, Accepted 15 April 2024, Published 19 June 2024. -- Correspondence: Narancio, R.; Instituto Nacional de Investigación Agropecuaria (INIA), Mejoramiento Genético y Biotecnología Vegetal. INIA Las Brujas, Ruta 48 Km 10, Canelones, Uruguay; email:rafael.narancio@agriculture.vic.gov.au --
520 $aABSTRACT.- Paspalum notatum Flüggé is a subtropical grass native from the Americas and one of the main components of the subtropical South American grasslands. Although P. notatum exhibits a high forage productivity and persistence, some limitations are observed in the species, such as forage quality due to high lignin content. The development of efficient tissue culture and plant transformation protocols in cultivars for forage production are necessary to embrace novel genetic improvement through transgenesis and genome editing. Efficient regeneration and transformation systems for many plant species can be hampered by genotype dependency, which can only be determined through extensive laboratory testing and evaluation. In this study, various factors extracted from protocols have been evaluated to establish efficient somatic embryogenesis, plant regeneration, and genetic transformation in apomictic initial variety INIA Sepé. Optimal somatic embryogenesis and regeneration from explant sources, such as mature seeds and meristematic tissue derived from in vitro propagated tillers, ranged between 79 and 88%. Microprojectile bombardment of two plasmids encoding a constitutively regulated reporter gene and nptII selectable marker expression constructs showed an average transformation efficiency of 10.3% from meristematic tissue as an explant source. Furthermore, the constitutive promoters, 35S and ZmUbi, were tested in GFP transient expression assays to evaluate optimal cassettes for transgene(s) expression. Translation of a wheat codon-optimized Cas9 sequence using Cas9:2A:tuGFP fusion protein was also tested in protoplasts. The information generated on promoter activity and Cas9 translation, and the establishment of an efficient transformation system, provided useful tools for future work in genetic engineering and genome editing in P. notatum cv. INIA Sepé. © The Society for In Vitro Biology 2024.
653 $aÁREA DE PASTURAS Y FORRAJES - INIA
653 $aC4 grasses
653 $aConstitutive promoters
653 $aMEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA
653 $aRegeneration
653 $aSomatic embryogenesis
653 $aTransformation
700 1 $aISENEGGER, D.
700 1 $aREYNO, R.
700 1 $aSPANGENBERG, G.
700 1 $aDALLA RIZZA, M.
773 $tIn Vitro Cellular and Developmental Biology - Plant, 2024. https://doi.org/10.1007/s11627-024-10434-z
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