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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
17/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. |
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Afiliación : |
VIRGINIA FERREIRA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARÍA J. PIANZZOLA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la República, Canelones, Uruguay; MARÍA L. TONDO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARÍA V. RODRÍGUEZ, Área Biología Vegetal (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; ELENA G. ORELLANO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARC VALSS, Center for Research in Agricultural Genomics, CSIC, IRTA, Barcelona, Spain; Department of Genetics, Universitat de Barcelona, Barcelona, Spain; MARÍA I. SIRI, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. |
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ISSN : |
1664-462X |
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DOI : |
10.3389/fpls.2017.01424 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. |
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Contenido : |
ABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.
© 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. MenosABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typic... Presentar Todo |
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Palabras claves : |
Bacterial wilt; Disease resistance; Latent infections; Plant breeding; Potato; Ralstonia solanacearum; Solanum commersonii. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03620naa a2200337 a 4500
001 1012685
005 2019-10-17
008 2017 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2017.01424$2DOI
100 1 $aFERREIRA, V.
245 $aInterspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017.
520 $aABSTRACT. Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato. © 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri.
653 $aBacterial wilt
653 $aDisease resistance
653 $aLatent infections
653 $aPlant breeding
653 $aPotato
653 $aRalstonia solanacearum
653 $aSolanum commersonii
700 1 $aPIANZZOLA, M.J.
700 1 $aVILARÓ, F.
700 1 $aGALVÁN, G.
700 1 $aTONDO, M.L.
700 1 $aRODRÍGUEZ, M.V.
700 1 $aORELLANO, E.G.
700 1 $aVALSS, M.
700 1 $aSIRI, M.I.
773 $tFrontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
|
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
26/02/2018 |
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Actualizado : |
26/02/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
LEONI, C.; BRUZZONE, J.; VILLAMIL, J.J.; MARTINEZ, C.; MONTELONGO, M.J.; BENTANCUR, O.; CONDE, P. |
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Afiliación : |
CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIANA BRUZZONE PIZZORNO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN JOSE VILLAMIL SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA CECILIA MARTINEZ ESTEFAN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ MONTELONGO, Universidad de la República (UdelaR)/ Facultad de Agronomía; OSCAR BENTANCUR, Universidad de la República (UdelaR)/ Facultad de Agronomía; ANA PAULA CONDE INNAMORATO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Percentage of anthracnose (Colletotrichum acutatum s.s.) acceptable in olives for the production of extra virgin olive oil. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Crop Protection, June 2018, v.108: 47-43. |
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DOI : |
10.1016/j.cropro.2018.02.013 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 February 2017; Revised 27 September 2017; Accepted 8 February 2018; Available online 22 February 2018.
Partial results were presented at 8th International Olive Symposium, Split, Croatia, 10?14 October 2016. |
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Contenido : |
Abstract
Anthracnose olive rot (AOR) is the main fruit disease of olives, causing direct yield losses and declining oil quality. Fruit infection occurs either in spring during flowering and fruit-set or in summer from the beginning of veraison to harvest. Conducive weather conditions (rain, elevated air relative humidity, air temperature above 20 °C) and the lack of effective chemical control strategies may compromise olive oil production. To quantify AOR effect on olive oil quality and to establish threshold levels, olive oil was extracted from olives with increasing disease incidence (from 0 to 30%), along three consecutive seasons (2012?2014) in two cultivars (cv. Arbeqina and cv. Frantoio) and disease severity index was also determined.
© 2018 Elsevier Ltd. All rights reserved. |
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Palabras claves : |
ARBEQUINA; FRANTOIO; OIL FREE ACIDITY; OLIVE OIL QUALITY; SOAPY OLIVE. |
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Thesagro : |
ACEITE OLIVA; CULTIVARES; OLEA EUROPAEA; OLIVOS; URUGUAY. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 01969naa a2200337 a 4500
001 1058155
005 2018-02-26
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.cropro.2018.02.013$2DOI
100 1 $aLEONI, C.
245 $aPercentage of anthracnose (Colletotrichum acutatum s.s.) acceptable in olives for the production of extra virgin olive oil.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 15 February 2017; Revised 27 September 2017; Accepted 8 February 2018; Available online 22 February 2018. Partial results were presented at 8th International Olive Symposium, Split, Croatia, 10?14 October 2016.
520 $aAbstract Anthracnose olive rot (AOR) is the main fruit disease of olives, causing direct yield losses and declining oil quality. Fruit infection occurs either in spring during flowering and fruit-set or in summer from the beginning of veraison to harvest. Conducive weather conditions (rain, elevated air relative humidity, air temperature above 20 °C) and the lack of effective chemical control strategies may compromise olive oil production. To quantify AOR effect on olive oil quality and to establish threshold levels, olive oil was extracted from olives with increasing disease incidence (from 0 to 30%), along three consecutive seasons (2012?2014) in two cultivars (cv. Arbeqina and cv. Frantoio) and disease severity index was also determined. © 2018 Elsevier Ltd. All rights reserved.
650 $aACEITE OLIVA
650 $aCULTIVARES
650 $aOLEA EUROPAEA
650 $aOLIVOS
650 $aURUGUAY
653 $aARBEQUINA
653 $aFRANTOIO
653 $aOIL FREE ACIDITY
653 $aOLIVE OIL QUALITY
653 $aSOAPY OLIVE
700 1 $aBRUZZONE, J.
700 1 $aVILLAMIL, J.J.
700 1 $aMARTINEZ, C.
700 1 $aMONTELONGO, M.J.
700 1 $aBENTANCUR, O.
700 1 $aCONDE, P.
773 $tCrop Protection, June 2018$gv.108: 47-43.
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