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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
03/12/2020 |
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Actualizado : |
03/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LLANES ALVAREZ, Y.; PEÑA BÁRZAGA, I.; BATISTA-LE RIVEREND, L.; PACHECO, R.; ZAMORA RODRÍGUEZ, V.; BENÍTEZ-GALEANO, M.J.; RIVAS, F.; BERTALMIO, A.; HERNÁNDEZ RODRÍGUEZ, L. |
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Afiliación : |
YILIAN LLANES-ALVAREZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; INÉS PEÑA-BÁRZAGA, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; LOCHY BATISTA-LE RIVEREND, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; RONAL PACHECO, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, México.; VICTORIA ZAMORA-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; MARÍA JOSÉ BENÍTEZ-GALEANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Laboratorio de Virología Molecular, Universidad de La República, Uruguay.; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Prevalence of mild citrus tristeza virus isolates of the T30 genotype in Cuban commercial citrus fields after the dissemination of huanglongbing. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Crop Protection, 2021, v. 140, art. 105422. DOI: https://doi.org/10.1016/j.cropro.2020.105422 |
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Páginas : |
11 p. |
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DOI : |
10.1016/j.cropro.2020.105422 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 5 May 2020; Received in revised form 8 October 2020; Accepted 12 October 2020; Available online 23 October 2020. |
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Contenido : |
Huanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained by the shift in the management programs implemented for CTV and HLB control in Cuba. MenosHuanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained ... Presentar Todo |
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Palabras claves : |
BIOLOGICAL CHARACTERIZATION; CLOSTEROVIRIDAE; CTV; GENOTYPING; MCA13; SEROLOGICAL CHARACTERIZATION. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02768naa a2200325 a 4500
001 1061537
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.cropro.2020.105422$2DOI
100 1 $aLLANES ALVAREZ, Y.
245 $aPrevalence of mild citrus tristeza virus isolates of the T30 genotype in Cuban commercial citrus fields after the dissemination of huanglongbing.$h[electronic resource]
260 $c2020
300 $a11 p.
500 $aArticle history: Received 5 May 2020; Received in revised form 8 October 2020; Accepted 12 October 2020; Available online 23 October 2020.
520 $aHuanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained by the shift in the management programs implemented for CTV and HLB control in Cuba.
653 $aBIOLOGICAL CHARACTERIZATION
653 $aCLOSTEROVIRIDAE
653 $aCTV
653 $aGENOTYPING
653 $aMCA13
653 $aSEROLOGICAL CHARACTERIZATION
700 1 $aPEÑA BÁRZAGA, I.
700 1 $aBATISTA-LE RIVEREND, L.
700 1 $aPACHECO, R.
700 1 $aZAMORA RODRÍGUEZ, V.
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aRIVAS, F.
700 1 $aBERTALMIO, A.
700 1 $aHERNÁNDEZ RODRÍGUEZ, L.
773 $tCrop Protection, 2021$gv. 140, art. 105422. DOI: https://doi.org/10.1016/j.cropro.2020.105422
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Registro original : |
INIA Treinta y Tres (TT) |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/06/2018 |
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Actualizado : |
22/07/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PEREIRA, J.; BERNAL, J.; MARTINELLI, L.; VILLAMIL, J.J.; CONDE, P. |
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Afiliación : |
J. PEREIRA, Universidad de la República (UdelaR)/ Facultad de Agronomía; J. BERNAL, Universidad de la República (UdelaR)/ Facultad de Agronomía; L. MARTINELLI, Universidad de la República (UdelaR)/ Facultad de Agronomía; JUAN JOSE VILLAMIL SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA PAULA CONDE INNAMORATO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Original olive genotypes found in Uruguay identified by morphological and molecular markers. |
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Complemento del título : |
Conference Paper. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Acta Horticulturae, May 2018, N° 1199, p. 7-13. |
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Serie : |
(Acta Horticulturae; 1199) |
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ISBN : |
978-94-62611-95-5 |
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ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2018.1199.2 |
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Idioma : |
Inglés |
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Notas : |
In: Acta Horticulturae (ISHS) 1199: VIII International Olive Symposium. Editors: S. Perica, G. Vuletin Selak, T. Klepo, L. Ferguson, L. Sebastiani. |
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Contenido : |
Abstract
Montevideo, the capital city of Uruguay, founded by Spaniards in the 18th century, and Colonia del Sacramento, established by the Portuguese in the 17th century, are sources of olive diversity. A study and analysis of this unexplored heritage was initiated 10 years ago, using morphological and molecular tools. After a primary geographical and historical survey, 360 Olea europaea fruit and leaf samples were collected. After morphological analysis of 11 stone characters, the samples could not be matched with any cultivars in databases from Mediterranean countries. When a set of 14 pairs of microsatellite primers was applied, nine different multilocus genotypes were detected that were not recorded in the databases. In agreement with the colonization process and olive tree introduction routes into South America, two groups of genotypes were distinguished: a) seven ?genetic mosaic? genotypes related to genotypes from Spain and Portugal, introduced to Uruguay in areas under Spanish control, and b) two genotypes with one of two alleles in less than 70% with molecular variants found in Hispanic and Portuguese cultivars. Because of their height and trunk size, these trees were assumed to be older. Based on this information, we conclude that Uruguay has preserved a unique and original gene pool, currently productive and adapted to local soil and climatic conditions. This pool is a heritage of global interest, suitable for commercial and cultural purposes.
@ International Society for Horticultural Science. MenosAbstract
Montevideo, the capital city of Uruguay, founded by Spaniards in the 18th century, and Colonia del Sacramento, established by the Portuguese in the 17th century, are sources of olive diversity. A study and analysis of this unexplored heritage was initiated 10 years ago, using morphological and molecular tools. After a primary geographical and historical survey, 360 Olea europaea fruit and leaf samples were collected. After morphological analysis of 11 stone characters, the samples could not be matched with any cultivars in databases from Mediterranean countries. When a set of 14 pairs of microsatellite primers was applied, nine different multilocus genotypes were detected that were not recorded in the databases. In agreement with the colonization process and olive tree introduction routes into South America, two groups of genotypes were distinguished: a) seven ?genetic mosaic? genotypes related to genotypes from Spain and Portugal, introduced to Uruguay in areas under Spanish control, and b) two genotypes with one of two alleles in less than 70% with molecular variants found in Hispanic and Portuguese cultivars. Because of their height and trunk size, these trees were assumed to be older. Based on this information, we conclude that Uruguay has preserved a unique and original gene pool, currently productive and adapted to local soil and climatic conditions. This pool is a heritage of global interest, suitable for commercial and cultural purposes.
@ International Soci... Presentar Todo |
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Palabras claves : |
ANCIENT OLIVE TREES; EVOLUTION; MOLECULAR; MORPHOLOGICAL MARKERS. |
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Thesagro : |
OLIVOS; URUGUAY. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02555naa a2200301 a 4500
001 1058727
005 2018-07-22
008 2018 bl uuuu u00u1 u #d
020 $a978-94-62611-95-5
022 $a0567-7572 (print) / 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2018.1199.2$2DOI
100 1 $aPEREIRA, J.
245 $aOriginal olive genotypes found in Uruguay identified by morphological and molecular markers.$h[electronic resource]
260 $c2018
490 $a(Acta Horticulturae; 1199)
500 $aIn: Acta Horticulturae (ISHS) 1199: VIII International Olive Symposium. Editors: S. Perica, G. Vuletin Selak, T. Klepo, L. Ferguson, L. Sebastiani.
520 $aAbstract Montevideo, the capital city of Uruguay, founded by Spaniards in the 18th century, and Colonia del Sacramento, established by the Portuguese in the 17th century, are sources of olive diversity. A study and analysis of this unexplored heritage was initiated 10 years ago, using morphological and molecular tools. After a primary geographical and historical survey, 360 Olea europaea fruit and leaf samples were collected. After morphological analysis of 11 stone characters, the samples could not be matched with any cultivars in databases from Mediterranean countries. When a set of 14 pairs of microsatellite primers was applied, nine different multilocus genotypes were detected that were not recorded in the databases. In agreement with the colonization process and olive tree introduction routes into South America, two groups of genotypes were distinguished: a) seven ?genetic mosaic? genotypes related to genotypes from Spain and Portugal, introduced to Uruguay in areas under Spanish control, and b) two genotypes with one of two alleles in less than 70% with molecular variants found in Hispanic and Portuguese cultivars. Because of their height and trunk size, these trees were assumed to be older. Based on this information, we conclude that Uruguay has preserved a unique and original gene pool, currently productive and adapted to local soil and climatic conditions. This pool is a heritage of global interest, suitable for commercial and cultural purposes. @ International Society for Horticultural Science.
650 $aOLIVOS
650 $aURUGUAY
653 $aANCIENT OLIVE TREES
653 $aEVOLUTION
653 $aMOLECULAR
653 $aMORPHOLOGICAL MARKERS
700 1 $aBERNAL, J.
700 1 $aMARTINELLI, L.
700 1 $aVILLAMIL, J.J.
700 1 $aCONDE, P.
773 $tActa Horticulturae, May 2018, N° 1199, p. 7-13.
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