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| 19. |  | Bottaro, C.; Zavala, F.; Rabuffetti, A.; Castells, D.; Elizondo, J.; Marchesi, E. Efecto de la fertilización mineral NP en la producción estacional de pasturas naturales en algunos tipos de suelos : 1er año ln: Congreso Nacional de Producción Animal, 1 : 1973 abr : Paysandu Madalena, F.E. ; Rucks, C. ; Azzarini, M. (coord). [Memorias]. Paysandu (Uruguay): Facultad de Agronomía, 1973. p1-21| Biblioteca(s): INIA La Estanzuela. |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
23/08/2018 |
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Actualizado : |
23/08/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
ABREO, E.; VALLE, D.; MUJICA, V.; ALTIER, N. |
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Afiliación : |
EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIANA VALLE LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Pathogenicity and virulence factors of Lysinibacillus xylanilyticus and Bacillus spp. towards Argyrotaenia sphaleropa larvae (Lepidoptera). |
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Complemento del título : |
Original contribution. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Journal of Applied Entomology, 2018, p.1-11. |
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DOI : |
10.1111/jen.12539 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 28 January 2018 / Accepted: 3 June 2018 / First published: 04 July 2018. |
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Contenido : |
Abstract
Argyrotaenia sphaleropa is a lepidopteran pest in deciduous fruit orchards in Uruguay. The primary control method of these pests is the use of chemical insecticides; however, alternative strategies, friendlier to the environment and compatible with a regional programme of integrated pest management, are definitely needed. Biological control with the use of local entomopathogenic microorganisms could be a sustainable solution. In this study, a biological screening was conducted to characterize spore‐forming bacilli for their capacity to kill larvae of this species. Bacillus thuringiensis, B. pumilus, B. aryabhattai, B. amyloliquefaciens and Lysinibacillus xylanilyticus were identified based on sequence analyses of 16S and partial GyrB gene. Lysinibacilus xylanilyticus showed the highest mortality towards first‐instar larvae fed with diet treated with spore suspensions. Specific virulence factors like proteinaceous parasporal inclusions were observed not only in isolates of B. thuringiensis but also in L. xylanilyticus. Ordering of isolates based on multivariate analysis of putative secondary virulence factors like biosurfactant and biofilm production, haemolytic activity and motility, separated the virulent isolate of L. xylanilyticus. This is the first report of L. xylanilyticus causing mortality in A. sphaleropa or any other member of Lepidoptera. A search for more virulent strains of this species is envisaged.
@© 2018 Blackwell Verlag GmbH |
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Palabras claves : |
16S; BIOFILM; CRY PROTEINS; HAEMOLYSIS; INTEGRATES PEST MANAGEMENT. |
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Thesagro : |
TORTRICIDAE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02319naa a2200253 a 4500
001 1058944
005 2018-08-23
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1111/jen.12539$2DOI
100 1 $aABREO, E.
245 $aPathogenicity and virulence factors of Lysinibacillus xylanilyticus and Bacillus spp. towards Argyrotaenia sphaleropa larvae (Lepidoptera).$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 28 January 2018 / Accepted: 3 June 2018 / First published: 04 July 2018.
520 $aAbstract Argyrotaenia sphaleropa is a lepidopteran pest in deciduous fruit orchards in Uruguay. The primary control method of these pests is the use of chemical insecticides; however, alternative strategies, friendlier to the environment and compatible with a regional programme of integrated pest management, are definitely needed. Biological control with the use of local entomopathogenic microorganisms could be a sustainable solution. In this study, a biological screening was conducted to characterize spore‐forming bacilli for their capacity to kill larvae of this species. Bacillus thuringiensis, B. pumilus, B. aryabhattai, B. amyloliquefaciens and Lysinibacillus xylanilyticus were identified based on sequence analyses of 16S and partial GyrB gene. Lysinibacilus xylanilyticus showed the highest mortality towards first‐instar larvae fed with diet treated with spore suspensions. Specific virulence factors like proteinaceous parasporal inclusions were observed not only in isolates of B. thuringiensis but also in L. xylanilyticus. Ordering of isolates based on multivariate analysis of putative secondary virulence factors like biosurfactant and biofilm production, haemolytic activity and motility, separated the virulent isolate of L. xylanilyticus. This is the first report of L. xylanilyticus causing mortality in A. sphaleropa or any other member of Lepidoptera. A search for more virulent strains of this species is envisaged. @© 2018 Blackwell Verlag GmbH
650 $aTORTRICIDAE
653 $a16S
653 $aBIOFILM
653 $aCRY PROTEINS
653 $aHAEMOLYSIS
653 $aINTEGRATES PEST MANAGEMENT
700 1 $aVALLE, D.
700 1 $aMUJICA, V.
700 1 $aALTIER, N.
773 $tJournal of Applied Entomology, 2018, p.1-11.
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