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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
11/06/2020 |
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Actualizado : |
22/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DO CARMO, M.; CARDOZO, G.; MECATTI, F.; SOCA, P.; HIRATA, M. |
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Afiliación : |
MARTÍN DO CARMO, Departamento de Sistemas Agrarios y Paisajes Culturales, Centro Universitario de la Región Este, Rocha, Uruguay.; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FULVIA MECATTI, Department of Statistics, Università degli Studi di Milano-Bicocca.; PABLO SOCA, Universidad de la República, School of Agronomy, EEMAC.; MASAHIKO HIRATA, Department of Animal and Grassland Sciences, University of Miyazaki. |
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Título : |
Number of samples for accurate visual estimation of mean herbage mass in Campos grasslands. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Agronomy Journal, July 2020, Vol. 112(4), p. 2734-2740. Doi: https://doi.org/10.1002/agj2.20237 |
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DOI : |
DOI: 10.1002/agj2.20237 |
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Idioma : |
Inglés |
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Notas : |
Article history: Manuscript received: 23 October 2019. Manuscript revised: 23 March 2020. Manuscript accepted: 01 April 2020. Accepted manuscript online: 09 April 2020. Version of Record online: 19 May 2020. Published July 2020. |
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Contenido : |
The number of samples is a major issue when estimating the mean herbage mass of grazed paddocks. The aim of this study was to assess the number of samples required for accurate visual estimation of mean herbage mass in relation to the herbage mass heterogeneity and size of paddocks. Data were collected across scales of space and time (273 sampling events) from paddocks on Campos grasslands in Uruguay, using the visual estimation technique. The mean herbage mass of the paddocks ranged from 270 to 6350 kg DM ha−1 with coefficient of variation (CV) of 0.13 to 1.26. Twenty-four events representing four levels of herbage mass heterogeneity (CV = 0.3, 0.5, 0.7 and 1.0) × three levels of paddock size (small, 5?13 ha; medium, 41?67 ha; large, 100?140 ha) were chosen (two replicates per group), and analyzed for the probability that the estimation error exceeded 10% of the mean (10% error probability) using the bootstrap technique. The number of samples required for
controlling the 10% error probability below 0.1 increased gradually from 50 to 150 per paddock as the CV increased from 0.3 to 0.7, then sharply to 350 until the CV increased to 1.0, with no effect of paddock size. Taking account of the distribution of CV (< 0.7 in nearly 80% of the events), we propose a general recommendation to take a minimum of 150 samples per paddock for accurate estimation of mean herbage mass in Campos grasslands irrespective of the size of paddocks. |
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Palabras claves : |
ANIMAL PRODUCTION; E-TANIN; ESTIMACION DE BIOMASA; FIELD IMPROVEMENT; LOTUS PEDUNCULATUS; MEJORAMIENTO DE CAMPO; MEJORAMIENTO DE CAMPO NATURAL; PRODUCCIÓN ANIMAL. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02569naa a2200289 a 4500
001 1061106
005 2021-02-22
008 2020 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1002/agj2.20237$2DOI
100 1 $aDO CARMO, M.
245 $aNumber of samples for accurate visual estimation of mean herbage mass in Campos grasslands.$h[electronic resource]
260 $c2020
500 $aArticle history: Manuscript received: 23 October 2019. Manuscript revised: 23 March 2020. Manuscript accepted: 01 April 2020. Accepted manuscript online: 09 April 2020. Version of Record online: 19 May 2020. Published July 2020.
520 $aThe number of samples is a major issue when estimating the mean herbage mass of grazed paddocks. The aim of this study was to assess the number of samples required for accurate visual estimation of mean herbage mass in relation to the herbage mass heterogeneity and size of paddocks. Data were collected across scales of space and time (273 sampling events) from paddocks on Campos grasslands in Uruguay, using the visual estimation technique. The mean herbage mass of the paddocks ranged from 270 to 6350 kg DM ha−1 with coefficient of variation (CV) of 0.13 to 1.26. Twenty-four events representing four levels of herbage mass heterogeneity (CV = 0.3, 0.5, 0.7 and 1.0) × three levels of paddock size (small, 5?13 ha; medium, 41?67 ha; large, 100?140 ha) were chosen (two replicates per group), and analyzed for the probability that the estimation error exceeded 10% of the mean (10% error probability) using the bootstrap technique. The number of samples required for controlling the 10% error probability below 0.1 increased gradually from 50 to 150 per paddock as the CV increased from 0.3 to 0.7, then sharply to 350 until the CV increased to 1.0, with no effect of paddock size. Taking account of the distribution of CV (< 0.7 in nearly 80% of the events), we propose a general recommendation to take a minimum of 150 samples per paddock for accurate estimation of mean herbage mass in Campos grasslands irrespective of the size of paddocks.
653 $aANIMAL PRODUCTION
653 $aE-TANIN
653 $aESTIMACION DE BIOMASA
653 $aFIELD IMPROVEMENT
653 $aLOTUS PEDUNCULATUS
653 $aMEJORAMIENTO DE CAMPO
653 $aMEJORAMIENTO DE CAMPO NATURAL
653 $aPRODUCCIÓN ANIMAL
700 1 $aCARDOZO, G.
700 1 $aMECATTI, F.
700 1 $aSOCA, P.
700 1 $aHIRATA, M.
773 $tAgronomy Journal, July 2020, Vol. 112(4), p. 2734-2740. Doi: https://doi.org/10.1002/agj2.20237
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INIA Treinta y Tres (TT) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
05/12/2018 |
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Actualizado : |
26/08/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
RUBIO, L.; HERNÁNDEZ, L.; BENÍTEZ, M. J.; ARRUABARRENA, A.; RIVAS, F.; COLINA, R.; MAESO, D. |
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Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARÍA JOSÉ BENÍTEZ GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Biological and molecular characterization of Uruguayan citrus tristeza virus field isolates. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Journal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105. |
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ISSN : |
1125-4653 // Online ISSN 2239-7264 |
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DOI : |
10.1007/s42161-018-0149-0 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019.
Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República.
Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06). |
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Contenido : |
ABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay.
© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018 MenosABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The su... Presentar Todo |
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Palabras claves : |
BIOLOGICAL INDEXING; CITRUS TRISTEZA VIRUS (CTV); CLOSTEROVIRIDAE; CLOSTEROVIRUS; PHYLOGENETIC ANALYSIS. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03065naa a2200301 a 4500
001 1059343
005 2019-08-26
008 2019 bl uuuu u00u1 u #d
022 $a1125-4653 // Online ISSN 2239-7264
024 7 $a10.1007/s42161-018-0149-0$2DOI
100 1 $aRUBIO, L.
245 $aBiological and molecular characterization of Uruguayan citrus tristeza virus field isolates.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019. Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República. Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06).
520 $aABSTRACT. Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018
650 $aCITRUS
653 $aBIOLOGICAL INDEXING
653 $aCITRUS TRISTEZA VIRUS (CTV)
653 $aCLOSTEROVIRIDAE
653 $aCLOSTEROVIRUS
653 $aPHYLOGENETIC ANALYSIS
700 1 $aHERNÁNDEZ, L.
700 1 $aBENÍTEZ, M. J.
700 1 $aARRUABARRENA, A.
700 1 $aRIVAS, F.
700 1 $aCOLINA, R.
700 1 $aMAESO, D.
773 $tJournal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105.
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