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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
12/07/2022 |
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Actualizado : |
12/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
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Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
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Complemento del título : |
Chapter 16. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
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ISBN : |
Online: 978-1-0716-2301-5 |
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DOI : |
10.1007/978-1-0716-2301-5_16 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
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Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02176naa a2200349 a 4500
001 1063414
005 2022-07-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_16$2DOI
100 1 $aKALDS, P.
245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource]
260 $c2022
520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock.
650 $aCABRAS
650 $aOVEJA
650 $aPRODUCCION DE CARNE
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aGoats
653 $aKnockout
653 $aMeat production
653 $aMicroinjection
653 $aMSTN
653 $aSheep
653 $aSmall ruminants
700 1 $aCRISPO, M.
700 1 $aTESSON, L.
700 1 $aANEGÓN, I.
700 1 $aCHEN KEY, Y.
700 1 $aWANG, X.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
11/11/2019 |
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Actualizado : |
01/06/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MONDRAGÓN-ANCELMO, J.; GARCÍA HERNÁNDEZ, P.; ROJO RUBIO, R.; DOMÍNGUEZ VARA, I.A.; DEL CAMPO, M.; NAPOLITANO, F. |
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Afiliación : |
JAIME MONDRAGÓN-ANCELMO, Centro Universitario Temascaltepec, Universidad Autónoma del Estado de México, Toluca, México; PATRICIA GARCÍA HERNÁNDEZ, Centro Universitario Temascaltepec , Universidad Autónoma del Estado de México , Toluca , México.; ROLANDO ROJO RUBIO ., Centro Universitario Temascaltepec , Universidad Autónoma del Estado de México , Toluca , México; IGNACIO ARTURO DOMÍNGUEZ VARA, Facultad de Medicina Veterinaria y Zootecnia , Universidad Autónoma del Estado de México , Toluca , México.; MARCIA DEL CAMPO GIGENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO NAPOLITANO, Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali , Università degli Studi della Basilicata , Potenza , Italy. |
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Título : |
Small Flocks Show Higher Levels of Welfare in Mexican Semi-Intensive Sheep Farming Systems. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Journal of Applied Animal Welfare Science, 2 July 2020, Volume 23, Issue 3, Pages 348-355. Doi: https://doi.org/10.1080/10888705.2019.1618303 |
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DOI : |
10.1080/10888705.2019.1618303 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published online: 06 Jun 2019. Acknowledgments:Research funded by Universidad Autónoma del Estado de México (Grant 3949/2015SF). Thanks are due to the sheep
farmers participating to the study for their hospitality and the provision of information and data. |
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Contenido : |
ABSTRACT: In order to assess the level of sheep welfare in small traditional farms as compared with farms of increased size we evaluated several animal-based parameters and applied a modified Animal Needs Index (ANI) protocol in farms located in the North-East of the country. We selected ten sheep farms: 5 farms had less than 20 ewes per flock (Group L20), whereas 5 farms had more than 20 ewes per flock (Group M20). Based on the recordings performed using the ANI scheme Group L20 constantly showed higher scores as compared with Group M20. In Group M20 a higher proportion of animals was affected by skin and wool damages, low body condition, tail mutilation, ocular discharge, nasal discharge, diarrhoea (P < 0.05) and lameness (P < 0.01). We observed a higher percentage of non-anaemic animals in group L20 and a higher percentage of anaemic animals in Group M20 (P < 0.01). Although based on a small sample, our results showed that several animal-based measures were able to discriminate the two groups of farms with higher levels of animal welfare detected in small flocks. |
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Palabras claves : |
ANIMAL NEEDS INDEX; ANIMAL WELFARE; ANIMAL-BASED MEASURES; AWIN; SHEEP. |
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Thesagro : |
BIENESTAR ANIMAL. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02262naa a2200277 a 4500
001 1060401
005 2020-06-01
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1080/10888705.2019.1618303$2DOI
100 1 $aMONDRAGÓN-ANCELMO, J.
245 $aSmall Flocks Show Higher Levels of Welfare in Mexican Semi-Intensive Sheep Farming Systems.$h[electronic resource]
260 $c2020
500 $aArticle history: Published online: 06 Jun 2019. Acknowledgments:Research funded by Universidad Autónoma del Estado de México (Grant 3949/2015SF). Thanks are due to the sheep farmers participating to the study for their hospitality and the provision of information and data.
520 $aABSTRACT: In order to assess the level of sheep welfare in small traditional farms as compared with farms of increased size we evaluated several animal-based parameters and applied a modified Animal Needs Index (ANI) protocol in farms located in the North-East of the country. We selected ten sheep farms: 5 farms had less than 20 ewes per flock (Group L20), whereas 5 farms had more than 20 ewes per flock (Group M20). Based on the recordings performed using the ANI scheme Group L20 constantly showed higher scores as compared with Group M20. In Group M20 a higher proportion of animals was affected by skin and wool damages, low body condition, tail mutilation, ocular discharge, nasal discharge, diarrhoea (P < 0.05) and lameness (P < 0.01). We observed a higher percentage of non-anaemic animals in group L20 and a higher percentage of anaemic animals in Group M20 (P < 0.01). Although based on a small sample, our results showed that several animal-based measures were able to discriminate the two groups of farms with higher levels of animal welfare detected in small flocks.
650 $aBIENESTAR ANIMAL
653 $aANIMAL NEEDS INDEX
653 $aANIMAL WELFARE
653 $aANIMAL-BASED MEASURES
653 $aAWIN
653 $aSHEEP
700 1 $aGARCÍA HERNÁNDEZ, P.
700 1 $aROJO RUBIO, R.
700 1 $aDOMÍNGUEZ VARA, I.A.
700 1 $aDEL CAMPO, M.
700 1 $aNAPOLITANO, F.
773 $tJournal of Applied Animal Welfare Science, 2 July 2020, Volume 23, Issue 3, Pages 348-355. Doi: https://doi.org/10.1080/10888705.2019.1618303
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