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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
12/07/2022 |
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Actualizado : |
12/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
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Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
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Complemento del título : |
Chapter 16. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
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ISBN : |
Online: 978-1-0716-2301-5 |
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DOI : |
10.1007/978-1-0716-2301-5_16 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
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Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02176naa a2200349 a 4500
001 1063414
005 2022-07-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_16$2DOI
100 1 $aKALDS, P.
245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource]
260 $c2022
520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock.
650 $aCABRAS
650 $aOVEJA
650 $aPRODUCCION DE CARNE
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aGoats
653 $aKnockout
653 $aMeat production
653 $aMicroinjection
653 $aMSTN
653 $aSheep
653 $aSmall ruminants
700 1 $aCRISPO, M.
700 1 $aTESSON, L.
700 1 $aANEGÓN, I.
700 1 $aCHEN KEY, Y.
700 1 $aWANG, X.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/11/2020 |
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Actualizado : |
27/11/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MARQUES, C. B.; GOLDBERG, V.; CIAPPESONI, G. |
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Afiliación : |
CAMILA BALCONI MARQUES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA GOLDBERG BIANCHI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Genetic parameters for production traits, resistance and resilience to Nematode parasites under different worm burden challenges in Corriedale sheep. (Research paper) |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Veterinary Parasitology, November 2020, Volume 287, Article number 109272. Doi: https://doi.org/10.1016/j.vetpar.2020.109272 |
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ISSN : |
0304-4017 |
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DOI : |
10.1016/j.vetpar.2020.109272 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 24 June 2020; Received in revised form 11 October 2020; Accepted 11 October 2020; Available online 15 October 2020. |
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Contenido : |
ABSTRACT.
Characterized as the most important gastrointestinal parasite (GIP) in the world, Haemonchus contortus is also the most predominant and pathogenic genus in Uruguay, causing high morbidity and mortality rates in sheep flocks. For approximately 26 years resistance to GIP has been included in the National Genetic Evaluation (NGE) of Corriedale and Merino breeds, using Faecal Egg Count (FEC) as the selection criterion. The aim of the present study was to estimate genetic parameters for FEC, post-weaning Body Weight (BW), Packed Cell Volume (PCV), FAMACHA© and Body Condition Score (BCS), to determine the possibility to include these traits in the NGE of GIP resistance and resilience, as complementary selection criteria and to investigate the sensitivity of breeding values to different environmental worm burden challenges. Data were collected on 19,510 lambs from 2000 to 2009 in 24 Corriedale studs that participated in the NGE of sheep in Uruguay. (Co)variances components were estimated using a multi-trait animal model performing a Bayesian analysis. Posterior means estimated for heritability (h2) were of low to moderate magnitude (between 0.10 and 0.33) for the traits analysed. Genetic correlations (rg) were not significantly different from zero for BW- Loge (FEC + 100), BW-FAMACHA©, BW-PCV, Loge (FEC + 100)-PCV, BCS-FAMACHA©, moderate favourable rg for Loge (FEC + 100)-FAMACHA and PCV-BCS (0.55, 0.41) and high favourable rg for BW-BCS (0.66). These results indicate the possibility to complement selection programs incorporating BW, Loge (FEC + 100), FAMACHA©, PCV and BCS in order to select resistant and resilient animal to GIP. Furthermore, genetic parameters for BW and Loge (FEC + 100) to different environmental worm burden challenge was investigated. Two contemporary groups of low worm environment (Loge (FEC + 100) below 6.40) and a high worm environment (Loge (FEC + 100) above 6.75) were considered. In this model, h2 estimated for BW and Loge (FEC + 100) were found to be not statistically different between low and high environmental worm burden, furthermore, high correlations between traits at both environments were also presented.
© 2020 Elsevier B.V. MenosABSTRACT.
Characterized as the most important gastrointestinal parasite (GIP) in the world, Haemonchus contortus is also the most predominant and pathogenic genus in Uruguay, causing high morbidity and mortality rates in sheep flocks. For approximately 26 years resistance to GIP has been included in the National Genetic Evaluation (NGE) of Corriedale and Merino breeds, using Faecal Egg Count (FEC) as the selection criterion. The aim of the present study was to estimate genetic parameters for FEC, post-weaning Body Weight (BW), Packed Cell Volume (PCV), FAMACHA© and Body Condition Score (BCS), to determine the possibility to include these traits in the NGE of GIP resistance and resilience, as complementary selection criteria and to investigate the sensitivity of breeding values to different environmental worm burden challenges. Data were collected on 19,510 lambs from 2000 to 2009 in 24 Corriedale studs that participated in the NGE of sheep in Uruguay. (Co)variances components were estimated using a multi-trait animal model performing a Bayesian analysis. Posterior means estimated for heritability (h2) were of low to moderate magnitude (between 0.10 and 0.33) for the traits analysed. Genetic correlations (rg) were not significantly different from zero for BW- Loge (FEC + 100), BW-FAMACHA©, BW-PCV, Loge (FEC + 100)-PCV, BCS-FAMACHA©, moderate favourable rg for Loge (FEC + 100)-FAMACHA and PCV-BCS (0.55, 0.41) and high favourable rg for BW-BCS (0.66). These results indicate the... Presentar Todo |
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Palabras claves : |
Faecal egg count; Genetic correlation; Haemonchus contortus; Heritability. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03171naa a2200229 a 4500
001 1061523
005 2020-11-27
008 2020 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $a10.1016/j.vetpar.2020.109272$2DOI
100 1 $aMARQUES, C. B.
245 $aGenetic parameters for production traits, resistance and resilience to Nematode parasites under different worm burden challenges in Corriedale sheep. (Research paper)$h[electronic resource]
260 $c2020
500 $aArticle history: Received 24 June 2020; Received in revised form 11 October 2020; Accepted 11 October 2020; Available online 15 October 2020.
520 $aABSTRACT. Characterized as the most important gastrointestinal parasite (GIP) in the world, Haemonchus contortus is also the most predominant and pathogenic genus in Uruguay, causing high morbidity and mortality rates in sheep flocks. For approximately 26 years resistance to GIP has been included in the National Genetic Evaluation (NGE) of Corriedale and Merino breeds, using Faecal Egg Count (FEC) as the selection criterion. The aim of the present study was to estimate genetic parameters for FEC, post-weaning Body Weight (BW), Packed Cell Volume (PCV), FAMACHA© and Body Condition Score (BCS), to determine the possibility to include these traits in the NGE of GIP resistance and resilience, as complementary selection criteria and to investigate the sensitivity of breeding values to different environmental worm burden challenges. Data were collected on 19,510 lambs from 2000 to 2009 in 24 Corriedale studs that participated in the NGE of sheep in Uruguay. (Co)variances components were estimated using a multi-trait animal model performing a Bayesian analysis. Posterior means estimated for heritability (h2) were of low to moderate magnitude (between 0.10 and 0.33) for the traits analysed. Genetic correlations (rg) were not significantly different from zero for BW- Loge (FEC + 100), BW-FAMACHA©, BW-PCV, Loge (FEC + 100)-PCV, BCS-FAMACHA©, moderate favourable rg for Loge (FEC + 100)-FAMACHA and PCV-BCS (0.55, 0.41) and high favourable rg for BW-BCS (0.66). These results indicate the possibility to complement selection programs incorporating BW, Loge (FEC + 100), FAMACHA©, PCV and BCS in order to select resistant and resilient animal to GIP. Furthermore, genetic parameters for BW and Loge (FEC + 100) to different environmental worm burden challenge was investigated. Two contemporary groups of low worm environment (Loge (FEC + 100) below 6.40) and a high worm environment (Loge (FEC + 100) above 6.75) were considered. In this model, h2 estimated for BW and Loge (FEC + 100) were found to be not statistically different between low and high environmental worm burden, furthermore, high correlations between traits at both environments were also presented. © 2020 Elsevier B.V.
653 $aFaecal egg count
653 $aGenetic correlation
653 $aHaemonchus contortus
653 $aHeritability
700 1 $aGOLDBERG, V.
700 1 $aCIAPPESONI, G.
773 $tVeterinary Parasitology, November 2020, Volume 287, Article number 109272. Doi: https://doi.org/10.1016/j.vetpar.2020.109272
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