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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
15/10/2014 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LADO, J.; RODRIGO, J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Analysis of ethylene biosynthesis and perception during postharvest cold storage of Marsh and Star Ruby grapefruits. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Food Science and Technology International, 2015, v.21, no.7, p. 537-546. |
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ISSN : |
1082-0132 |
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DOI : |
10.1177/1082013214553810 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 31 July 2014 // Accepted 8 September 2014 // Published online 3 October 2014. |
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Contenido : |
ABSTRACT.
Grapefruits are among the citrus species more sensitive to cold and develop chilling injury symptoms during prolonged postharvest storage at temperatures lower than 8 C?10 C. The plant hormone ethylene has been described either to protect or potentiate chilling injury development in citrus whereas little is known about transcriptional regulation of ethylene biosynthesis, perception and response during cold storage and how the hormone is regulating its own perception and signaling cascade. Then, the objective of the present study was to explore the transcriptional changes in the expression of ethylene biosynthesis, receptors and response genes during cold storage of the white Marsh and the red Star Ruby grapefruits. The effect of the ethylene action inhibitor, 1-MCP, was evaluated to investigate the involvement of ethylene in the regulation of the genes of its own biosynthesis and perception pathway. Ethylene production was very low at the harvest time in fruits of both varieties and experienced only minor changes during storage. By contrast, inhibition of ethylene perception by 1-MCP markedly induced ethylene production, and this increase was highly stimulated during shelf-life at 20 C, as well as transcription of ACS and ACO. These results support the autoinhibitory regulation of ethylene in grapefruits, which acts mainly at the transcriptional level of ACS and ACO genes. Moreover, ethylene receptor1 and ethylene receptor3 were induced by cold while no clear role of ethylene was observed in the induction of ethylene receptors. However, ethylene appears to be implicated in the transcriptional regulation of ERFs both under cold storage and shelf-life. MenosABSTRACT.
Grapefruits are among the citrus species more sensitive to cold and develop chilling injury symptoms during prolonged postharvest storage at temperatures lower than 8 C?10 C. The plant hormone ethylene has been described either to protect or potentiate chilling injury development in citrus whereas little is known about transcriptional regulation of ethylene biosynthesis, perception and response during cold storage and how the hormone is regulating its own perception and signaling cascade. Then, the objective of the present study was to explore the transcriptional changes in the expression of ethylene biosynthesis, receptors and response genes during cold storage of the white Marsh and the red Star Ruby grapefruits. The effect of the ethylene action inhibitor, 1-MCP, was evaluated to investigate the involvement of ethylene in the regulation of the genes of its own biosynthesis and perception pathway. Ethylene production was very low at the harvest time in fruits of both varieties and experienced only minor changes during storage. By contrast, inhibition of ethylene perception by 1-MCP markedly induced ethylene production, and this increase was highly stimulated during shelf-life at 20 C, as well as transcription of ACS and ACO. These results support the autoinhibitory regulation of ethylene in grapefruits, which acts mainly at the transcriptional level of ACS and ACO genes. Moreover, ethylene receptor1 and ethylene receptor3 were induced by cold while no clear role... Presentar Todo |
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Palabras claves : |
CHILLING INJURY; COLD; ETHYLENE SIGNALING; PHYSIOLOGICAL DISORDER. |
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Thesagro : |
CITRUS; DAÑO POR HELADA; FRIO. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02573naa a2200265 a 4500
001 1051119
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
022 $a1082-0132
024 7 $a10.1177/1082013214553810$2DOI
100 1 $aLADO, J.
245 $aAnalysis of ethylene biosynthesis and perception during postharvest cold storage of Marsh and Star Ruby grapefruits.$h[electronic resource]
260 $c2015
500 $aArticle history: Received 31 July 2014 // Accepted 8 September 2014 // Published online 3 October 2014.
520 $aABSTRACT. Grapefruits are among the citrus species more sensitive to cold and develop chilling injury symptoms during prolonged postharvest storage at temperatures lower than 8 C?10 C. The plant hormone ethylene has been described either to protect or potentiate chilling injury development in citrus whereas little is known about transcriptional regulation of ethylene biosynthesis, perception and response during cold storage and how the hormone is regulating its own perception and signaling cascade. Then, the objective of the present study was to explore the transcriptional changes in the expression of ethylene biosynthesis, receptors and response genes during cold storage of the white Marsh and the red Star Ruby grapefruits. The effect of the ethylene action inhibitor, 1-MCP, was evaluated to investigate the involvement of ethylene in the regulation of the genes of its own biosynthesis and perception pathway. Ethylene production was very low at the harvest time in fruits of both varieties and experienced only minor changes during storage. By contrast, inhibition of ethylene perception by 1-MCP markedly induced ethylene production, and this increase was highly stimulated during shelf-life at 20 C, as well as transcription of ACS and ACO. These results support the autoinhibitory regulation of ethylene in grapefruits, which acts mainly at the transcriptional level of ACS and ACO genes. Moreover, ethylene receptor1 and ethylene receptor3 were induced by cold while no clear role of ethylene was observed in the induction of ethylene receptors. However, ethylene appears to be implicated in the transcriptional regulation of ERFs both under cold storage and shelf-life.
650 $aCITRUS
650 $aDAÑO POR HELADA
650 $aFRIO
653 $aCHILLING INJURY
653 $aCOLD
653 $aETHYLENE SIGNALING
653 $aPHYSIOLOGICAL DISORDER
700 1 $aRODRIGO, J.
700 1 $aZACARÍAS, L.
773 $tFood Science and Technology International, 2015$gv.21, no.7, p. 537-546.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
03/12/2020 |
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Actualizado : |
03/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
-- - -- |
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Autor : |
LLANES ALVAREZ, Y.; PEÑA BÁRZAGA, I.; BATISTA-LE RIVEREND, L.; PACHECO, R.; ZAMORA RODRÍGUEZ, V.; BENÍTEZ-GALEANO, M.J.; RIVAS, F.; BERTALMIO, A.; HERNÁNDEZ RODRÍGUEZ, L. |
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Afiliación : |
YILIAN LLANES-ALVAREZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; INÉS PEÑA-BÁRZAGA, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; LOCHY BATISTA-LE RIVEREND, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; RONAL PACHECO, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, México.; VICTORIA ZAMORA-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; MARÍA JOSÉ BENÍTEZ-GALEANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Laboratorio de Virología Molecular, Universidad de La República, Uruguay.; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Prevalence of mild citrus tristeza virus isolates of the T30 genotype in Cuban commercial citrus fields after the dissemination of huanglongbing. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Crop Protection, 2021, v. 140, art. 105422. DOI: https://doi.org/10.1016/j.cropro.2020.105422 |
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Páginas : |
11 p. |
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DOI : |
10.1016/j.cropro.2020.105422 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 5 May 2020; Received in revised form 8 October 2020; Accepted 12 October 2020; Available online 23 October 2020. |
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Contenido : |
Huanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained by the shift in the management programs implemented for CTV and HLB control in Cuba. MenosHuanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained ... Presentar Todo |
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Palabras claves : |
BIOLOGICAL CHARACTERIZATION; CLOSTEROVIRIDAE; CTV; GENOTYPING; MCA13; SEROLOGICAL CHARACTERIZATION. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02768naa a2200325 a 4500
001 1061537
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.cropro.2020.105422$2DOI
100 1 $aLLANES ALVAREZ, Y.
245 $aPrevalence of mild citrus tristeza virus isolates of the T30 genotype in Cuban commercial citrus fields after the dissemination of huanglongbing.$h[electronic resource]
260 $c2020
300 $a11 p.
500 $aArticle history: Received 5 May 2020; Received in revised form 8 October 2020; Accepted 12 October 2020; Available online 23 October 2020.
520 $aHuanglongbing (HLB) disease detection in Cuba in 2006 led to a turn in citrus management in the country, including the production of certified budwood in protected nurseries, eradication of symptomatic plants and old orchards, and chemical control of the vector Diaphorina citri. This dramatic shift in citrus farming could have affected the incidence and composition of citrus tristeza virus (CTV) populations. This work aimed to determine CTV incidence and characterize viral isolates from the main Cuban citrus-producing areas. A survey throughout the country was carried out which resulted in the collection of 27 CTV isolates. Results showed a decrease of CTV incidence in western and central areas of the country, but an increase in the East, compared to previous reports. Bioindexing showed that most of the isolates induced none or mild symptoms. Serological characterization using seven different CTV specific monoclonal antibodies (MAb) showed heterogeneity among the 27 isolates. Six out of twenty-seven were MCA13-reactive, a MAb associated to the presence of severe CTV isolates. Nevertheless, sequence comparison of the p20, p23 and p25 genes evidenced a high nucleotide sequence identity among isolates, and according to the phylogenetic analyses, all the sequences belonged to the CTV T30 genotype. Thus, our results showed a decrease in the CTV incidence in the surveyed areas and the broad dissemination of mild CTV isolates of the T30 genotype, which could be partially explained by the shift in the management programs implemented for CTV and HLB control in Cuba.
653 $aBIOLOGICAL CHARACTERIZATION
653 $aCLOSTEROVIRIDAE
653 $aCTV
653 $aGENOTYPING
653 $aMCA13
653 $aSEROLOGICAL CHARACTERIZATION
700 1 $aPEÑA BÁRZAGA, I.
700 1 $aBATISTA-LE RIVEREND, L.
700 1 $aPACHECO, R.
700 1 $aZAMORA RODRÍGUEZ, V.
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aRIVAS, F.
700 1 $aBERTALMIO, A.
700 1 $aHERNÁNDEZ RODRÍGUEZ, L.
773 $tCrop Protection, 2021$gv. 140, art. 105422. DOI: https://doi.org/10.1016/j.cropro.2020.105422
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