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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
13/01/2020 |
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Actualizado : |
01/06/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OBERTI, H.; DALLA RIZZA, M.; REYNO, R.; MURCHIO, S.; ALTIER, N.; ABREO, E. |
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Afiliación : |
HÉCTOR OBERTI RVAROLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Mycologia, 3 March 2020, Volume 112, Issue 2, Pages 230-243. Doi: https://doi.org/10.1080/00275514.2019.1694827 |
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DOI : |
10.1080/00275514.2019.1694827 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 Apr 2019 // Accepted 15 Nov 2019 // Published online: 07 Jan 2020. CONTACT: E. Abreo, eabreo@inia.org.uy. FUNDING: This work was supported by the National Institute of Agricultural Research (INIA) of Uruguay (grant numbers SA-24, BT-19). |
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Contenido : |
Claviceps species affecting Paspalum spp. are a serious problem, as they infect forage grasses such as Paspalum dilatatum and P. plicatulum, producing the ergot disease. The ascomycete C. paspali is known to be the pathogen responsible for this disease in both grasses. This fungus produces alkaloids, including ergot alkaloids and indole-diterpenes, that have potent neurotropic activities in mammals. A total of 32 isolates from Uruguay were obtained from infected P. dilatatum and P. plicatulum. Isolates were phylogenetically identified using partial sequences of the genes coding for the second largest subunit of RNA polymerase subunit II (RPB2), translation elongation factor 1-? (TEF1), ?-tubulin (TUB2), and the nuc rDNA 28S subunit (28S). Isolates were also genotyped by randomly amplified polymorphic DNA (RAPD) and presence of genes within the ergot alkaloid (EAS) and indole-diterpene (IDT) biosynthetic gene clusters. This study represents the first genetic characterization of several isolates of C. paspali. The results from this study provide insight into the genetic and genotypic diversity of Claviceps paspali present in P. dilatatum and suggest that isolates from P. plicatulum could be considered an ecological subspecies or specialized variant of C. paspali. Some of these isolates show hypothetical alkaloid genotypes never reported before. |
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Palabras claves : |
ERGOT; FINGERPRINTING; HOST SPECIATION; PASPALUM; PHYLOGENY; STAGGER. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02438naa a2200277 a 4500
001 1060598
005 2020-06-01
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1080/00275514.2019.1694827$2DOI
100 1 $aOBERTI, H.
245 $aDiversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 25 Apr 2019 // Accepted 15 Nov 2019 // Published online: 07 Jan 2020. CONTACT: E. Abreo, eabreo@inia.org.uy. FUNDING: This work was supported by the National Institute of Agricultural Research (INIA) of Uruguay (grant numbers SA-24, BT-19).
520 $aClaviceps species affecting Paspalum spp. are a serious problem, as they infect forage grasses such as Paspalum dilatatum and P. plicatulum, producing the ergot disease. The ascomycete C. paspali is known to be the pathogen responsible for this disease in both grasses. This fungus produces alkaloids, including ergot alkaloids and indole-diterpenes, that have potent neurotropic activities in mammals. A total of 32 isolates from Uruguay were obtained from infected P. dilatatum and P. plicatulum. Isolates were phylogenetically identified using partial sequences of the genes coding for the second largest subunit of RNA polymerase subunit II (RPB2), translation elongation factor 1-? (TEF1), ?-tubulin (TUB2), and the nuc rDNA 28S subunit (28S). Isolates were also genotyped by randomly amplified polymorphic DNA (RAPD) and presence of genes within the ergot alkaloid (EAS) and indole-diterpene (IDT) biosynthetic gene clusters. This study represents the first genetic characterization of several isolates of C. paspali. The results from this study provide insight into the genetic and genotypic diversity of Claviceps paspali present in P. dilatatum and suggest that isolates from P. plicatulum could be considered an ecological subspecies or specialized variant of C. paspali. Some of these isolates show hypothetical alkaloid genotypes never reported before.
653 $aERGOT
653 $aFINGERPRINTING
653 $aHOST SPECIATION
653 $aPASPALUM
653 $aPHYLOGENY
653 $aSTAGGER
700 1 $aDALLA RIZZA, M.
700 1 $aREYNO, R.
700 1 $aMURCHIO, S.
700 1 $aALTIER, N.
700 1 $aABREO, E.
773 $tMycologia, 3 March 2020, Volume 112, Issue 2, Pages 230-243. Doi: https://doi.org/10.1080/00275514.2019.1694827
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INIA Tacuarembó (TBO) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
23/12/2021 |
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Actualizado : |
23/12/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
LEITE, F.F.G.D.; ALVES, B.J.R.; NÓBREGA, G.N.; CORDEIRO, R.C.; CESÁRIO, F.V.; CAMBARERI, G.S.; FAVERIN, C.; DA SILVEIRA, J.G.; CIGANDA, V.; ARMACOLO, N.M.; ALECRIM, F.B.; RODRIGUES, R.D.A.R. |
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Afiliación : |
FERNANDA FIGUEIREDO GRANJA DORILÊO LEITE, Programa de Pós-Graduação em Geociências (Geoquímica), Departamento de Geoquímica, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil.; BRUNO JOSE RODRIGUES ALVES, Embrapa Agrobiologia, Seropédica, RJ, Brazil.; GABRIEL NUTO NÓBREGA, Programa de Pós-Graduação em Geociências (Geoquímica), Departamento de Geoquímica, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil.; RENATO CAMPELLO CORDEIRO, Programa de Pós-Graduação em Geociências (Geoquímica), Departamento de Geoquímica, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil.; FERNANDO VIEIRA CESÁRIO, Programa de Pós-Graduação em Engenharia de Biossistemas, Departamento de Engenharia Agrícola e Meio Ambiente, Universidade Federal Fluminense (UFF), Niterói, Brazil.; GUSTAVO SEBASTIAN CAMBARERI, Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce, Buenos Aires, Argentina.; CLAUDIA FAVERIN, Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce, Buenos Aires, Argentina; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Buenos Aires, Argentina.; JULIA GRAZIELA DA SILVEIRA, Departamento de Engenharia Florestal, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil.; VERONICA SOLANGE CIGANDA BRASCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATASSIA MAGALHÃES ARMACOLO, Departamento de Agronomia, Universidade Estadual de Londrina (UEL), Londrina, PR, Brazil.; FABIANO BARBOSA ALECRIM, Programa de Pós-Graduação em Geociências (Geoquímica), Departamento de Geoquímica, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil.; RENATO DE ARAGÃO RIBEIRO RODRIGUESA, Programa de Pós-Graduação em Geociências (Geoquímica), Departamento de Geoquímica, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil Embrapa Solos, R. Jardim Botânico, Rio de Janeiro, RJ, Brazil. |
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Título : |
Checking the progress of using the static chamber method for the measurement of greenhouse gases in Latin America. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Carbon Management, 2021. doi: https://doi.org/10.1080/17583004.2021.1995503 |
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Descripción física : |
Aragão |
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ISSN : |
1758-3004 |
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DOI : |
10.1080/17583004.2021.1995503 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published online: 27 Oct 2021.
Correspondence author: T Renato de Aragão Ribeiro Rodrigues, e-mail: renato.rodrigues@embrapa.br
Additional information: The authors thank the scholarship supported by the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS), the Global Research Alliance on Agricultural Greenhouse Gases (GRA) through their CLIFF-GRADS programme to FFGDL, and individual grants: CNPq N° 02/2020, process number 308444/2020-5 to RARR and the Research Support Foundation of the State of Rio de Janeiro - FAPERJ JCNE grant number E-26/202.757/2019 to GNN. |
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Contenido : |
ABSTRACT - Manual static chamber is one of the most widespread methods for the quantification of greenhouse gases (GHGs) emissions from agricultural systems. But there are concerns about the reliability of measurements and a necessity to assess the static chambers used in the studies performed in the countries of Latin America. Thus, this study aims to investigate the quality of the characteristics from the static chamber methodology, the confidence level of the GHG measurements, and recommend what should be improved. We conducted a systematic review of databases to identify peer-reviewed articles that used the static chamber method in Latin America. A total of 90 articles were analyzed and separated according to the publication year (2000-2008, 2009-2015, 2016-2020). We evaluated eight design and deployment chamber characteristics. The depth of base insertion and type of storage vial received the lowest scores, and the number of samples received the highest score. The description and reporting of the methodological procedures in the studies should be improved. The percentage of articles with high and medium confidence levels increased within the time, and the number of articles with low and very low confidence levels reduced. Scientists/Researchers should continue to follow the protocols, especially the most updated ones. © 2021 Informa UK Limited, trading as Taylor & Francis Group. |
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Palabras claves : |
GHG management; Greenhouse gas management; Greenhouses gas; Methodologies; Performance measurement; Protocols and standards; South America. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03163naa a2200385 a 4500
001 1062620
005 2021-12-23
008 2021 bl uuuu u00u1 u #d
022 $a1758-3004
024 7 $a10.1080/17583004.2021.1995503$2DOI
100 1 $aLEITE, F.F.G.D.
245 $aChecking the progress of using the static chamber method for the measurement of greenhouse gases in Latin America.$h[electronic resource]
260 $c2021
300 $cAragão
500 $aArticle history: Published online: 27 Oct 2021. Correspondence author: T Renato de Aragão Ribeiro Rodrigues, e-mail: renato.rodrigues@embrapa.br Additional information: The authors thank the scholarship supported by the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS), the Global Research Alliance on Agricultural Greenhouse Gases (GRA) through their CLIFF-GRADS programme to FFGDL, and individual grants: CNPq N° 02/2020, process number 308444/2020-5 to RARR and the Research Support Foundation of the State of Rio de Janeiro - FAPERJ JCNE grant number E-26/202.757/2019 to GNN.
520 $aABSTRACT - Manual static chamber is one of the most widespread methods for the quantification of greenhouse gases (GHGs) emissions from agricultural systems. But there are concerns about the reliability of measurements and a necessity to assess the static chambers used in the studies performed in the countries of Latin America. Thus, this study aims to investigate the quality of the characteristics from the static chamber methodology, the confidence level of the GHG measurements, and recommend what should be improved. We conducted a systematic review of databases to identify peer-reviewed articles that used the static chamber method in Latin America. A total of 90 articles were analyzed and separated according to the publication year (2000-2008, 2009-2015, 2016-2020). We evaluated eight design and deployment chamber characteristics. The depth of base insertion and type of storage vial received the lowest scores, and the number of samples received the highest score. The description and reporting of the methodological procedures in the studies should be improved. The percentage of articles with high and medium confidence levels increased within the time, and the number of articles with low and very low confidence levels reduced. Scientists/Researchers should continue to follow the protocols, especially the most updated ones. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
653 $aGHG management
653 $aGreenhouse gas management
653 $aGreenhouses gas
653 $aMethodologies
653 $aPerformance measurement
653 $aProtocols and standards
653 $aSouth America
700 1 $aALVES, B.J.R.
700 1 $aNÓBREGA, G.N.
700 1 $aCORDEIRO, R.C.
700 1 $aCESÁRIO, F.V.
700 1 $aCAMBARERI, G.S.
700 1 $aFAVERIN, C.
700 1 $aDA SILVEIRA, J.G.
700 1 $aCIGANDA, V.
700 1 $aARMACOLO, N.M.
700 1 $aALECRIM, F.B.
700 1 $aRODRIGUES, R.D.A.R.
773 $tCarbon Management, 2021. doi: https://doi.org/10.1080/17583004.2021.1995503
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