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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/12/2020 |
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Actualizado : |
21/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LOICK, N.; DIXON, E.; MATTHEWS, G.P.; MÜLLER, CH.; CIGANDA, V.; LÓPEZ-AIZPÚN, M.; REPULLO, M.A.; CARDENAS, L.M. |
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Afiliación : |
NADINE LOICK, Rothamsted Research, North Wyke, Okehampton, Devon EX20 2SB, UK; ELIZABETH DIXON, Rothamsted Research, North Wyke, Okehampton, Devon EX20 2SB, UK.; G. PETER MATTHEWS, School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK.; CHRISTOPH MÜLLER, Institute of Plant Ecology, Justus Liebig University Giessen, 35392 Giessen, German.; VERONICA SOLANGE CIGANDA BRASCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA LÓPEZ-AIZPÚN, LICA, Department of Chemistry, University of Navarre, Irunlarrea, 1-31008 Pamplona, Spain.; MIGUEL A. REPULLO, IFAPA, Area of Agriculture and Environment Centre Alameda del Obispo, Av. Menéndez Pidal s/n, Apdo 3092, 14080 Córdoba, Spain; LAURA M. CARDENAS, Rothamsted Research, North Wyke, Okehampton, Devon EX20 2SB, UK. |
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Título : |
Application of a triple 15N tracing technique to elucidate N transformations in a UK grassland soil. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Geoderma, 1 March 2021, Volume 385, Article number 114844. Doi: https://doi.org/10.1016/j.geoderma.2020.114844 |
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DOI : |
10.1016/j.geoderma.2020.114844 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 14 August 2020/ Revised 11 November 2020/ Accepted 13 November 2020/ Available online 2 December 2020. |
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Contenido : |
Abstract: To identify the production and consumption pathways and temporal dynamics of N2O emitted from soil, this study uses 15N-labelled substrate-N to quantify the underlying gross N transformation rates using the Ntrace analysis tool and link them to N-emissions. In three experiments twelve soil cores each were incubated in a lab incubation system to measure gaseous emissions, while parallel incubations under the same conditions were set up for destructive soil sampling at 7 time points. Using the triple labelling technique (applying NH4NO3 with either the NH4+-N or the NO3?-N, or both being 15N labelled), this study investigated the effects of 55, 70 and 85% water filled pore space (deemed to promote nitrification, both nitrification and denitrification, and denitrification, respectively) in a clay soil on gaseous N emissions and investigates the source and processes leading to N2O emissions. To assess the utilisation of applied NO3? vs. nitrified NO3? from applied NH4+, the 15N tracing tool Ntrace was used to quantify the rates of immobilisation of NO3? and NH4+, oxidation of NH4+, mineralisation of organic N and subsequent nitrification by the analysis of the 15N in the soil. Gross transformation rates were calculated, indicating the relative importance of added NO3? and NO3? derived from nitrified added NH4+. Results show an important contribution of heterotrophic nitrification (organic N oxidation to NO3?) which was highest at the 55% water filled pore space (WFPS), decreasing in its contribution to N-transformation processes with increasing WFPS, while nitrification (NH4+ oxidation to NO3?) was contributing the most at 70% WFPS. The contribution of denitrification increased with increasing WFPS, but only became dominant at 85% WFPS. While denitrification still showed to be most important at high and nitrification at lower WFPS, the actual % WFPS values were not as expected and highlight the fact that WFPS is a contributor, but not the sole/most important parameter determining the type of N-transformation processes taking place. MenosAbstract: To identify the production and consumption pathways and temporal dynamics of N2O emitted from soil, this study uses 15N-labelled substrate-N to quantify the underlying gross N transformation rates using the Ntrace analysis tool and link them to N-emissions. In three experiments twelve soil cores each were incubated in a lab incubation system to measure gaseous emissions, while parallel incubations under the same conditions were set up for destructive soil sampling at 7 time points. Using the triple labelling technique (applying NH4NO3 with either the NH4+-N or the NO3?-N, or both being 15N labelled), this study investigated the effects of 55, 70 and 85% water filled pore space (deemed to promote nitrification, both nitrification and denitrification, and denitrification, respectively) in a clay soil on gaseous N emissions and investigates the source and processes leading to N2O emissions. To assess the utilisation of applied NO3? vs. nitrified NO3? from applied NH4+, the 15N tracing tool Ntrace was used to quantify the rates of immobilisation of NO3? and NH4+, oxidation of NH4+, mineralisation of organic N and subsequent nitrification by the analysis of the 15N in the soil. Gross transformation rates were calculated, indicating the relative importance of added NO3? and NO3? derived from nitrified added NH4+. Results show an important contribution of heterotrophic nitrification (organic N oxidation to NO3?) which was highest at the 55% water filled pore space (WFPS),... Presentar Todo |
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Palabras claves : |
DENITRIFICATION; EMISIONES DE N20; HETEROTROPHIC NITRIFICATION; NITRIFICATION; NITROUS OXIDE. |
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Thesagro : |
DENITRIFICACION. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03134naa a2200301 a 4500
001 1061589
005 2021-05-21
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.geoderma.2020.114844$2DOI
100 1 $aLOICK, N.
245 $aApplication of a triple 15N tracing technique to elucidate N transformations in a UK grassland soil.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 14 August 2020/ Revised 11 November 2020/ Accepted 13 November 2020/ Available online 2 December 2020.
520 $aAbstract: To identify the production and consumption pathways and temporal dynamics of N2O emitted from soil, this study uses 15N-labelled substrate-N to quantify the underlying gross N transformation rates using the Ntrace analysis tool and link them to N-emissions. In three experiments twelve soil cores each were incubated in a lab incubation system to measure gaseous emissions, while parallel incubations under the same conditions were set up for destructive soil sampling at 7 time points. Using the triple labelling technique (applying NH4NO3 with either the NH4+-N or the NO3?-N, or both being 15N labelled), this study investigated the effects of 55, 70 and 85% water filled pore space (deemed to promote nitrification, both nitrification and denitrification, and denitrification, respectively) in a clay soil on gaseous N emissions and investigates the source and processes leading to N2O emissions. To assess the utilisation of applied NO3? vs. nitrified NO3? from applied NH4+, the 15N tracing tool Ntrace was used to quantify the rates of immobilisation of NO3? and NH4+, oxidation of NH4+, mineralisation of organic N and subsequent nitrification by the analysis of the 15N in the soil. Gross transformation rates were calculated, indicating the relative importance of added NO3? and NO3? derived from nitrified added NH4+. Results show an important contribution of heterotrophic nitrification (organic N oxidation to NO3?) which was highest at the 55% water filled pore space (WFPS), decreasing in its contribution to N-transformation processes with increasing WFPS, while nitrification (NH4+ oxidation to NO3?) was contributing the most at 70% WFPS. The contribution of denitrification increased with increasing WFPS, but only became dominant at 85% WFPS. While denitrification still showed to be most important at high and nitrification at lower WFPS, the actual % WFPS values were not as expected and highlight the fact that WFPS is a contributor, but not the sole/most important parameter determining the type of N-transformation processes taking place.
650 $aDENITRIFICACION
653 $aDENITRIFICATION
653 $aEMISIONES DE N20
653 $aHETEROTROPHIC NITRIFICATION
653 $aNITRIFICATION
653 $aNITROUS OXIDE
700 1 $aDIXON, E.
700 1 $aMATTHEWS, G.P.
700 1 $aMÜLLER, CH.
700 1 $aCIGANDA, V.
700 1 $aLÓPEZ-AIZPÚN, M.
700 1 $aREPULLO, M.A.
700 1 $aCARDENAS, L.M.
773 $tGeoderma, 1 March 2021, Volume 385, Article number 114844. Doi: https://doi.org/10.1016/j.geoderma.2020.114844
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
28/03/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DE ARMAS, S.; GALVÁN, G. A.; LAPAZ, M. I.; GONZÁLEZ-BARRIOS, P.; VICENTE, E.; PIANZZOLA, M. J.; SIRI, M. I. |
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Afiliación : |
STEFANIE DE ARMAS, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY.; Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; GUILLERMO A. GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur (CRS), Facultad de Agronomía, UDELAR, Canelones, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. LAPAZ, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, uy. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; PABLO GONZÁLEZ-BARRIOS, Departamento de Biometría, Estadística y Computación. Facultad de Agronomía, UDELAR, Montevideo, UY.; CARLOS ESTEBAN VICENTE CASTRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA J. PIANZZOLA, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. SIRI, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA). |
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Título : |
Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Plant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE |
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DOI : |
10.1094/PDIS-06-21-1140-RE |
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Idioma : |
Inglés |
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Notas : |
Published Online:27 Mar 2022.
Corresponding author: María I. Siri; Email: msiri@fq.edu.uy. |
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Contenido : |
Abstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens. MenosAbstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves... Presentar Todo |
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Palabras claves : |
ALLIUM CEPA; CENTER ROT; PANTOEA SPP; PATHOGEN DIVERSITY. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03015naa a2200265 a 4500
001 1062926
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1094/PDIS-06-21-1140-RE$2DOI
100 1 $aDE ARMAS, S.
245 $aPhylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay.$h[electronic resource]
260 $c2022
500 $aPublished Online:27 Mar 2022. Corresponding author: María I. Siri; Email: msiri@fq.edu.uy.
520 $aAbstract Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens.
653 $aALLIUM CEPA
653 $aCENTER ROT
653 $aPANTOEA SPP
653 $aPATHOGEN DIVERSITY
700 1 $aGALVÁN, G. A.
700 1 $aLAPAZ, M. I.
700 1 $aGONZÁLEZ-BARRIOS, P.
700 1 $aVICENTE, E.
700 1 $aPIANZZOLA, M. J.
700 1 $aSIRI, M. I.
773 $tPlant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE
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