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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/02/2021 |
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Actualizado : |
22/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MAESO, D.; FEDERICI, M.; MARTÍNEZ, A.; SILVERA, M.; GONCALVEZ, L. |
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Afiliación : |
DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A. MARTÍNEZ, Facultad de Ciencias, Universidad de la Repu?blica, Montevideo, Uruguay.; MARIANA SILVERA ORREGO, Facultad de Ciencias, Universidad de la Repu?blica, Montevideo, Uruguay.; ANA LUCIA GONCALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Studies on pear decline disease in Uruguay. [Conference paper]. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Acta Horticulturae, February 2021, N°1303, p. 343-350. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.48 |
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ISSN : |
0567-7572 (print); 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2021.1303.48 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. |
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Contenido : |
Abstract:
Pear decline (PD) caused by 'Candidatus Phytoplasma pyri' (subgroup 16SrX-C of apple proliferation or AP group of phytoplasmas) is an important pear disease (Pyrus communis L.). Pear production in Uruguay is based on 'Williams' plants initially grafted on quince that are planted deep and thus transformed into scion-rooted trees. Less vigorous rootstocks like Pyrus spp. and quinces that could be more susceptible to decline are beginning to be used for precocity. Surveys were conducted on plants grafted on different rootstocks to know if PD was present in the pear growing area of Uruguay (southern part near Montevideo city) by searching for premature reddening, upward rolling of leaves and decline. Phytoplasmas were observed in 35 of 70 samples using the DAPI (4',6-diamidino-2-fenilindol) staining test. Ten positive and 10 negative samples were analyzed by polymerase chain reaction (PCR) assay using AP-group specific primers f01/r01. A 930 bp amplicon was obtained only from all DAPI positive samples. The f01/r01 amplicon was sequenced and showed 100% identity with the 16S rRNA gene sequence of PD phytoplasma in the NCBI database. A relationship was observed between phytoplasma detection, reddening, psylla infestation and premature leaf fall in this survey in 1995. Presence of PD-symptoms and phytoplasma detection by PCR were also evaluated in an experiment where 20 pear scion/rootstock combinations were compared during 2005-2011. Plants of self-rooted 'Williams' or grafted onto OH×F 40 and OH×F 69 stocks showed less early reddening and leaf fall than plants grafted onto quince rootstocks. Phytoplasma detection in this trial varied among seasons and was not associated with the presence of symptoms. Phytoplasmas were detected in pear psylla insects during the whole 2009-2011 seasons in four pear orchards. An association between the effectiveness of psylla control, phytoplasma detection and early reddening and leaf fall was found in an experiment with traditional 'Williams' self-rooted plants in 2011. These results indicate the presence of PD phytoplasma in Uruguayan pear orchards and in psylla insects during the whole season.
@ International Society for Horticultural Science. MenosAbstract:
Pear decline (PD) caused by 'Candidatus Phytoplasma pyri' (subgroup 16SrX-C of apple proliferation or AP group of phytoplasmas) is an important pear disease (Pyrus communis L.). Pear production in Uruguay is based on 'Williams' plants initially grafted on quince that are planted deep and thus transformed into scion-rooted trees. Less vigorous rootstocks like Pyrus spp. and quinces that could be more susceptible to decline are beginning to be used for precocity. Surveys were conducted on plants grafted on different rootstocks to know if PD was present in the pear growing area of Uruguay (southern part near Montevideo city) by searching for premature reddening, upward rolling of leaves and decline. Phytoplasmas were observed in 35 of 70 samples using the DAPI (4',6-diamidino-2-fenilindol) staining test. Ten positive and 10 negative samples were analyzed by polymerase chain reaction (PCR) assay using AP-group specific primers f01/r01. A 930 bp amplicon was obtained only from all DAPI positive samples. The f01/r01 amplicon was sequenced and showed 100% identity with the 16S rRNA gene sequence of PD phytoplasma in the NCBI database. A relationship was observed between phytoplasma detection, reddening, psylla infestation and premature leaf fall in this survey in 1995. Presence of PD-symptoms and phytoplasma detection by PCR were also evaluated in an experiment where 20 pear scion/rootstock combinations were compared during 2005-2011. Plants of self-rooted 'Williams' or g... Presentar Todo |
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Palabras claves : |
Cacopsylla bidens; Candidatus Phytoplasma pyri; European pear; Scion-rootstock affinity. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 03270naa a2200253 a 4500
001 1061745
005 2021-02-22
008 2021 bl uuuu u00u1 u #d
022 $a0567-7572 (print); 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2021.1303.48$2DOI
100 1 $aMAESO, D.
245 $aStudies on pear decline disease in Uruguay. [Conference paper].$h[electronic resource]
260 $c2021
500 $aArticle history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein.
520 $aAbstract: Pear decline (PD) caused by 'Candidatus Phytoplasma pyri' (subgroup 16SrX-C of apple proliferation or AP group of phytoplasmas) is an important pear disease (Pyrus communis L.). Pear production in Uruguay is based on 'Williams' plants initially grafted on quince that are planted deep and thus transformed into scion-rooted trees. Less vigorous rootstocks like Pyrus spp. and quinces that could be more susceptible to decline are beginning to be used for precocity. Surveys were conducted on plants grafted on different rootstocks to know if PD was present in the pear growing area of Uruguay (southern part near Montevideo city) by searching for premature reddening, upward rolling of leaves and decline. Phytoplasmas were observed in 35 of 70 samples using the DAPI (4',6-diamidino-2-fenilindol) staining test. Ten positive and 10 negative samples were analyzed by polymerase chain reaction (PCR) assay using AP-group specific primers f01/r01. A 930 bp amplicon was obtained only from all DAPI positive samples. The f01/r01 amplicon was sequenced and showed 100% identity with the 16S rRNA gene sequence of PD phytoplasma in the NCBI database. A relationship was observed between phytoplasma detection, reddening, psylla infestation and premature leaf fall in this survey in 1995. Presence of PD-symptoms and phytoplasma detection by PCR were also evaluated in an experiment where 20 pear scion/rootstock combinations were compared during 2005-2011. Plants of self-rooted 'Williams' or grafted onto OH×F 40 and OH×F 69 stocks showed less early reddening and leaf fall than plants grafted onto quince rootstocks. Phytoplasma detection in this trial varied among seasons and was not associated with the presence of symptoms. Phytoplasmas were detected in pear psylla insects during the whole 2009-2011 seasons in four pear orchards. An association between the effectiveness of psylla control, phytoplasma detection and early reddening and leaf fall was found in an experiment with traditional 'Williams' self-rooted plants in 2011. These results indicate the presence of PD phytoplasma in Uruguayan pear orchards and in psylla insects during the whole season. @ International Society for Horticultural Science.
653 $aCacopsylla bidens
653 $aCandidatus Phytoplasma pyri
653 $aEuropean pear
653 $aScion-rootstock affinity
700 1 $aFEDERICI, M.
700 1 $aMARTÍNEZ, A.
700 1 $aSILVERA, M.
700 1 $aGONCALVEZ, L.
773 $tActa Horticulturae, February 2021, N°1303, p. 343-350. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.48
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
15/06/2022 |
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Actualizado : |
15/06/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
TSCHOPP, J.C.; MACAGNO, A.J.; MAPLETOFT, R.J.; MENCHACA, A.; BÓ, G. A. |
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Afiliación : |
JUAN CARLOS TSCHOPP, Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina; Instituto A.P. de Ciencias Básicas y Aplicadas, Medicina Veterinaria, Universidad Nacional de Villa María, Villa del Rosario, Córdoba, Argentina; ALEJANDRO J. MACAGNO, Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina; Instituto A.P. de Ciencias Básicas y Aplicadas, Medicina Veterinaria, Universidad Nacional de Villa María, Villa del Rosario, Córdoba, Argentina; REUBEN J. MAPLETOFT, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; GABRIEL A. BÓ, Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina; Instituto A.P. de Ciencias Básicas y Aplicadas, Medicina Veterinaria, Universidad Nacional de Villa María, Villa del Rosario, Córdoba, Argentina. |
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Título : |
Effect of the addition of GnRH and a second prostaglandin F2α treatment on pregnancy per artificial insemination in lactating dairy cows submitted to an estradiol/progesterone-based timed-AI protocol. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Theriogenology, 2022, Volume 188, Pages 63-70. doi: https://doi.org/10.1016/j.theriogenology.2022.05.019 |
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ISSN : |
0093-691X |
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DOI : |
10.1016/j.theriogenology.2022.05.019 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 February 2022; Received in revised form 23 May 2022; Accepted 24 May 2022; Available online 27 May 2022; To be published August 2022.
Corresponding author: Bó, G.A.; Instituto de Reproducción Animal Córdoba (IRAC), Zona Rural General Paz, Córdoba, Uruguay; email:gabrielbo62@gmail.com --
This research was supported by Fondo Nacional de Ciencia y Tecnología (FONCYT PICT 2017?4550), Instituto de Investigación Universidad Nacional de Villa María (UNVM), and Instituto de Reproducción Animal de Córdoba (IRAC). |
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Contenido : |
ABSTRACT - Two experiments determined whether the addition of GnRH at the beginning of an estradiol (E2)/progesterone (P4)- based synchronization protocol and/or a second dose of prostaglandin F2α (PGF2α) the day before P4 device removal improves pregnancy rate in lactating dairy cows. On Day 0, all cows received a CIDR-B device and 2 mg i.m. estradiol benzoate, and half received 200 μg i.m. gonadorelin acetate (GnRH). On Day 7, cows were further subdivided to receive PGF2α (500 μg i.m. cloprostenol) or no PGF2α treatment. On Day 8, CIDR-B were removed, and all cows received PGF2α, 1 mg estradiol cypionate and 400 IU eCG i.m., and an estrus detection aid. Experiment 1 was designed to evaluate the effect of treatments on follicular development from P4 device removal to ovulation, expression of estrus, time of ovulation and serum P4 concentrations. Cows (n = 76) were examined by ultrasonography and bled for serum P4 determinations every 12 h from the time of P4 device removal but were not inseminated. In Experiment 2, all cows (n = 1036) were inseminated based on estrus detection using tail-paint. Cows with >50% of the paint rubbed-off by 48 h after P4 device removal were inseminated at that time, whereas those not in estrus received 100 μg i.m. of GnRH and were inseminated 12 h later. In Experiment 1, the interval from P4 device removal to ovulation was 71.7 ± 1.5 h and did not differ among groups. However, cows that received 2 injections of PGF2α had a greater (P < 0.01) estrus rate and lower (P < 0.01) P4 concentrations at 48 h after P4 device removal than those that received 1 PGF2α (estrus rate: 86.8% vs 68.4% and P4 concentration: 0.12 ± 0.01 vs 0.36 ± 0.07, for 2 and 1 PGF2α, respectively). In Experiment 2, estrus rate was also influenced by the number of PGF2α treatments, regardless of whether cows received or did not receive GnRH on Day 0 (2 PGF2α: 84.7%, 438/517 vs 1 PGF2α: 65.7%, 341/519; P < 0.01). Furthermore, there was a GnRH treatment by number of PGF2α treatments interaction (P < 0.05) on P/AI that was attributed to greater (P < 0.05) P/AI in cows that received GnRH on Day 0 and 2 PGF2α than in the other three treatment groups (EB+1 PGF2α: 45.2%, 119/263; EB+2 PGF2α: 45.8%, 119/260; EB + GnRH + 1 PGF2α: 45.7%, 117/256 and EB + GnRH + 2 PGF2α: 57.2%, 147/257). It was concluded that the addition of GnRH on Day 0 and a second dose of PGF2α the day before P4 device removal improves P/AI in lactating dairy cows synchronized with an estradiol/P4-based protocol. © 2022 Elsevier Inc. MenosABSTRACT - Two experiments determined whether the addition of GnRH at the beginning of an estradiol (E2)/progesterone (P4)- based synchronization protocol and/or a second dose of prostaglandin F2α (PGF2α) the day before P4 device removal improves pregnancy rate in lactating dairy cows. On Day 0, all cows received a CIDR-B device and 2 mg i.m. estradiol benzoate, and half received 200 μg i.m. gonadorelin acetate (GnRH). On Day 7, cows were further subdivided to receive PGF2α (500 μg i.m. cloprostenol) or no PGF2α treatment. On Day 8, CIDR-B were removed, and all cows received PGF2α, 1 mg estradiol cypionate and 400 IU eCG i.m., and an estrus detection aid. Experiment 1 was designed to evaluate the effect of treatments on follicular development from P4 device removal to ovulation, expression of estrus, time of ovulation and serum P4 concentrations. Cows (n = 76) were examined by ultrasonography and bled for serum P4 determinations every 12 h from the time of P4 device removal but were not inseminated. In Experiment 2, all cows (n = 1036) were inseminated based on estrus detection using tail-paint. Cows with >50% of the paint rubbed-off by 48 h after P4 device removal were inseminated at that time, whereas those not in estrus received 100 μg i.m. of GnRH and were inseminated 12 h later. In Experiment 1, the interval from P4 device removal to ovulation was 71.7 ± 1.5 h and did not differ among groups. However, cows that received 2 injectio... Presentar Todo |
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Palabras claves : |
Dairy cows; Estrus; GnRH; Ovulation; PLATAFORMA DE SALUD ANIMAL; Timed-AI. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 04132naa a2200277 a 4500
001 1063305
005 2022-06-15
008 2022 bl uuuu u00u1 u #d
022 $a0093-691X
024 7 $a10.1016/j.theriogenology.2022.05.019$2DOI
100 1 $aTSCHOPP, J.C.
245 $aEffect of the addition of GnRH and a second prostaglandin F2α treatment on pregnancy per artificial insemination in lactating dairy cows submitted to an estradiol/progesterone-based timed-AI protocol.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 23 February 2022; Received in revised form 23 May 2022; Accepted 24 May 2022; Available online 27 May 2022; To be published August 2022. Corresponding author: Bó, G.A.; Instituto de Reproducción Animal Córdoba (IRAC), Zona Rural General Paz, Córdoba, Uruguay; email:gabrielbo62@gmail.com -- This research was supported by Fondo Nacional de Ciencia y Tecnología (FONCYT PICT 2017?4550), Instituto de Investigación Universidad Nacional de Villa María (UNVM), and Instituto de Reproducción Animal de Córdoba (IRAC).
520 $aABSTRACT - Two experiments determined whether the addition of GnRH at the beginning of an estradiol (E2)/progesterone (P4)- based synchronization protocol and/or a second dose of prostaglandin F2α (PGF2α) the day before P4 device removal improves pregnancy rate in lactating dairy cows. On Day 0, all cows received a CIDR-B device and 2 mg i.m. estradiol benzoate, and half received 200 μg i.m. gonadorelin acetate (GnRH). On Day 7, cows were further subdivided to receive PGF2α (500 μg i.m. cloprostenol) or no PGF2α treatment. On Day 8, CIDR-B were removed, and all cows received PGF2α, 1 mg estradiol cypionate and 400 IU eCG i.m., and an estrus detection aid. Experiment 1 was designed to evaluate the effect of treatments on follicular development from P4 device removal to ovulation, expression of estrus, time of ovulation and serum P4 concentrations. Cows (n = 76) were examined by ultrasonography and bled for serum P4 determinations every 12 h from the time of P4 device removal but were not inseminated. In Experiment 2, all cows (n = 1036) were inseminated based on estrus detection using tail-paint. Cows with >50% of the paint rubbed-off by 48 h after P4 device removal were inseminated at that time, whereas those not in estrus received 100 μg i.m. of GnRH and were inseminated 12 h later. In Experiment 1, the interval from P4 device removal to ovulation was 71.7 ± 1.5 h and did not differ among groups. However, cows that received 2 injections of PGF2α had a greater (P < 0.01) estrus rate and lower (P < 0.01) P4 concentrations at 48 h after P4 device removal than those that received 1 PGF2α (estrus rate: 86.8% vs 68.4% and P4 concentration: 0.12 ± 0.01 vs 0.36 ± 0.07, for 2 and 1 PGF2α, respectively). In Experiment 2, estrus rate was also influenced by the number of PGF2α treatments, regardless of whether cows received or did not receive GnRH on Day 0 (2 PGF2α: 84.7%, 438/517 vs 1 PGF2α: 65.7%, 341/519; P < 0.01). Furthermore, there was a GnRH treatment by number of PGF2α treatments interaction (P < 0.05) on P/AI that was attributed to greater (P < 0.05) P/AI in cows that received GnRH on Day 0 and 2 PGF2α than in the other three treatment groups (EB+1 PGF2α: 45.2%, 119/263; EB+2 PGF2α: 45.8%, 119/260; EB + GnRH + 1 PGF2α: 45.7%, 117/256 and EB + GnRH + 2 PGF2α: 57.2%, 147/257). It was concluded that the addition of GnRH on Day 0 and a second dose of PGF2α the day before P4 device removal improves P/AI in lactating dairy cows synchronized with an estradiol/P4-based protocol. © 2022 Elsevier Inc.
653 $aDairy cows
653 $aEstrus
653 $aGnRH
653 $aOvulation
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTimed-AI
700 1 $aMACAGNO, A.J.
700 1 $aMAPLETOFT, R.J.
700 1 $aMENCHACA, A.
700 1 $aBÓ, G. A.
773 $tTheriogenology, 2022, Volume 188, Pages 63-70. doi: https://doi.org/10.1016/j.theriogenology.2022.05.019
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