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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
24/10/2014 |
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Actualizado : |
04/05/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DEL CAMPO, M.; BRITO, G.; SOARES DE LIMA, J.M.; VAZ MARTINS, D.; SAÑUDO, C.; SAN JULIAN, R.; HERNANDEZ, P.; MONTOSSI, F. |
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Afiliación : |
MARCIA DEL CAMPO GIGENA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO WALTER BRITO DIAZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; JUAN MANUEL SOARES DE LIMA LAPETINA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; DANIEL VAZ MARTINS GIGENA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; C. SAÑUDO, University of Zaragoza, España; ROBERTO SAN JULIAN SANCHEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; P. HERNADEZ, Polytechnical University of Valencia, España; FABIO MARCELO MONTOSSI PORCHILE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Effects of feeding strategies including different proportion of pasture and concentrate, on carcass and meat quality traits in Uruguayan steers. |
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Fecha de publicación : |
2008 |
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Fuente / Imprenta : |
Meat Science, Volume 80, Issue 3, November 2008, Pages 753-760. https://doi.org/10.1016/j.meatsci.2008.03.026 |
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DOI : |
10.1016/j.meatsci.2008.03.026 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 3 January 2008 // Received in revised form 15 March 2008 //Accepted 15 March 2008. Acknowledgements: This experiment was part of a cooperation agreement amongINIA Uruguay, INIA Spain and the Spanish Agency for InternationalCooperation (AECI, Spain); with the participation of IRTA Cataluña(Spain); University of Zaragoza (Spain) and the National Meat Institute (INAC, Uruguay).We wish to thank AECI (Spain) for the financial support of thefirst author’s Doctoral studies in Spain.We would like to thank the R&D&I inguistic Assistance Officeat the Politechnical University of Valencia for their help in revisingand correcting this paper. |
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Contenido : |
Eighty four steers were randomly assigned to three pasture treatments with increasing levels of grain (T1: 0%; T2: 0.6%; T3: 1.2% of live weight) and to an ad libitum concentrate treatment, T4, to study the effects on carcass and meat quality. Animals were slaughtered with 500 kg of average live weight per
treatment. Average daily gain increased with increasing levels of energy, determining different slaughter dates. Intermediate treatments showed higher carcass weight than T1. T4 and T3 had a higher weight of valuable cuts than T1 and T4. Pistolas from T4 had a higher fat proportion and lower bone percentage. Increasing levels of energy in diet decreased fat yellowness. After 20 days of aging, T4 had the lowest muscle a* values and shear force was higher for T4 than for T1. With pastures finishing strategy, no adverse effects on meat quality were detected and tenderness was enhanced. |
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Palabras claves : |
BEEF; CARCASS TRAITS; DIET; MEAT QUALITY. |
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Thesagro : |
CALIDAD DE CARNE; CARNE; URUGUAY. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02501naa a2200313 a 4500
001 1051294
005 2020-05-04
008 2008 bl uuuu u00u1 u #d
024 7 $a10.1016/j.meatsci.2008.03.026$2DOI
100 1 $aDEL CAMPO, M.
245 $aEffects of feeding strategies including different proportion of pasture and concentrate, on carcass and meat quality traits in Uruguayan steers.
260 $c2008
500 $aArticle history: Received 3 January 2008 // Received in revised form 15 March 2008 //Accepted 15 March 2008. Acknowledgements: This experiment was part of a cooperation agreement amongINIA Uruguay, INIA Spain and the Spanish Agency for InternationalCooperation (AECI, Spain); with the participation of IRTA Cataluña(Spain); University of Zaragoza (Spain) and the National Meat Institute (INAC, Uruguay).We wish to thank AECI (Spain) for the financial support of thefirst author’s Doctoral studies in Spain.We would like to thank the R&D&I inguistic Assistance Officeat the Politechnical University of Valencia for their help in revisingand correcting this paper.
520 $aEighty four steers were randomly assigned to three pasture treatments with increasing levels of grain (T1: 0%; T2: 0.6%; T3: 1.2% of live weight) and to an ad libitum concentrate treatment, T4, to study the effects on carcass and meat quality. Animals were slaughtered with 500 kg of average live weight per treatment. Average daily gain increased with increasing levels of energy, determining different slaughter dates. Intermediate treatments showed higher carcass weight than T1. T4 and T3 had a higher weight of valuable cuts than T1 and T4. Pistolas from T4 had a higher fat proportion and lower bone percentage. Increasing levels of energy in diet decreased fat yellowness. After 20 days of aging, T4 had the lowest muscle a* values and shear force was higher for T4 than for T1. With pastures finishing strategy, no adverse effects on meat quality were detected and tenderness was enhanced.
650 $aCALIDAD DE CARNE
650 $aCARNE
650 $aURUGUAY
653 $aBEEF
653 $aCARCASS TRAITS
653 $aDIET
653 $aMEAT QUALITY
700 1 $aBRITO, G.
700 1 $aSOARES DE LIMA, J.M.
700 1 $aVAZ MARTINS, D.
700 1 $aSAÑUDO, C.
700 1 $aSAN JULIAN, R.
700 1 $aHERNANDEZ, P.
700 1 $aMONTOSSI, F.
773 $tMeat Science, Volume 80, Issue 3, November 2008, Pages 753-760. https://doi.org/10.1016/j.meatsci.2008.03.026
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INIA Tacuarembó (TBO) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
12/07/2022 |
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Actualizado : |
12/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
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Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
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Complemento del título : |
Chapter 16. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
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ISBN : |
Online: 978-1-0716-2301-5 |
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DOI : |
10.1007/978-1-0716-2301-5_16 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
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Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02176naa a2200349 a 4500
001 1063414
005 2022-07-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_16$2DOI
100 1 $aKALDS, P.
245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource]
260 $c2022
520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock.
650 $aCABRAS
650 $aOVEJA
650 $aPRODUCCION DE CARNE
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aGoats
653 $aKnockout
653 $aMeat production
653 $aMicroinjection
653 $aMSTN
653 $aSheep
653 $aSmall ruminants
700 1 $aCRISPO, M.
700 1 $aTESSON, L.
700 1 $aANEGÓN, I.
700 1 $aCHEN KEY, Y.
700 1 $aWANG, X.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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