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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
20/11/2020 |
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Actualizado : |
20/11/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BELLÉ, C.; RAMOS, R.F.; KASPARY, T. E.; DOSSIN, M.F.; BRIDA, A.L.; ANTONIOLLI, Z.I. |
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Afiliación : |
CRISTIANO BELLÉ, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Santa Maria, Rio Grande do Sul, Brasil.; RODRIGO FERRAZ RAMOS, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Santa Maria, Rio Grande do Sul, Brasil.; TIAGO EDU KASPARY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIANA FERNEDA DOSSIN, Universidade Federal de Santa Maria, Brasil.; ANDRESSA LIMA DE BRIDA, Faculdade de Administração e Ciências Contábeis, Campo Mourão, Paraná, Brasil.; ZAIDA INÊS ANTONIOLLI, Universidade Federal de Santa Maria, Brasil. |
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Título : |
Host susceptibility of hordeum vulgare to Meloidogyne graminicola. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Nematropica, 2020, vol. 50, p.96-100. |
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Idioma : |
Inglés |
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Notas : |
Article history: Accepted for publication:11/VII/2019 /Recibido: Aceptado para publicación: 2/IX/2020 |
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Contenido : |
Abstract:
Barley (Hordeum vulgare) is an important crop used for human consumption and mainly by the malt industry. Recently, a species of the root-knot nematode (Meloidogyne graminicola) was found and recorded to parasitize barley roots in Brazil. In this context, the objective of this work was to evaluate the susceptibility of 17 barley genotypes to M. graminicola in the greenhouse. Barley genotypes were individually inoculated with 5,000 eggs + second stage juveniles (J2) of Meloidogyne and kept in a greenhouse. After 60 days, the roots of each plant were evaluated for gall number, number of nematodes per root gram and reproduction factor (RF = final population / initial population). All barley genotypes evaluated behaved as susceptible to M. graminicola. The cultivar 'Scarlett' had the lowest RF (14.9), while the 'BRS Itanema' resulted in the highest RF (24.5).
Resumo:
A cevada (Hordeum vulgare) é uma importante cultura utilizada na alimentação humana e principalmente pela indústria de maltes. Recentemente, uma espécie do nematoide-das-galhas (Meloidogyne graminicola) foi encontrada e registrada parasitando raízes de plantas de cevada no Brasil.Nesse contexto, o objetivo do trabalho foi avaliar, em casa de vegetação, a suscetibilidade de 17 cultivares de cevada a M. graminicola. As cultivares de cevada foram individualmente inoculadas com 5.000 ovos +juvenis do segundo estádio (J2) de Meloidogyne mantidas em casa de vegetação. Após 60 dias, as raízes de cada planta foram avaliadas quanto ao número de galhas, população final e fator de reprodução (FR=população final/população inicial). Todas as cultivares de cevada avaliadas comportaram-se como
suscetíveis a M. graminicola. A cultivar ?Scarlett? apresentou o menor FR (14,9), enquanto que a ?BRS
Itanema? resultou no maior FR (24,5). MenosAbstract:
Barley (Hordeum vulgare) is an important crop used for human consumption and mainly by the malt industry. Recently, a species of the root-knot nematode (Meloidogyne graminicola) was found and recorded to parasitize barley roots in Brazil. In this context, the objective of this work was to evaluate the susceptibility of 17 barley genotypes to M. graminicola in the greenhouse. Barley genotypes were individually inoculated with 5,000 eggs + second stage juveniles (J2) of Meloidogyne and kept in a greenhouse. After 60 days, the roots of each plant were evaluated for gall number, number of nematodes per root gram and reproduction factor (RF = final population / initial population). All barley genotypes evaluated behaved as susceptible to M. graminicola. The cultivar 'Scarlett' had the lowest RF (14.9), while the 'BRS Itanema' resulted in the highest RF (24.5).
Resumo:
A cevada (Hordeum vulgare) é uma importante cultura utilizada na alimentação humana e principalmente pela indústria de maltes. Recentemente, uma espécie do nematoide-das-galhas (Meloidogyne graminicola) foi encontrada e registrada parasitando raízes de plantas de cevada no Brasil.Nesse contexto, o objetivo do trabalho foi avaliar, em casa de vegetação, a suscetibilidade de 17 cultivares de cevada a M. graminicola. As cultivares de cevada foram individualmente inoculadas com 5.000 ovos +juvenis do segundo estádio (J2) de Meloidogyne mantidas em casa de vegetação. Após 60 dias, as raízes de cada planta fora... Presentar Todo |
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Palabras claves : |
BARLEY; REPRODUCTION; ROOT-KNOT NEMATODE; SUSCEPTIBILITY. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02572naa a2200241 a 4500
001 1061514
005 2020-11-20
008 2020 bl uuuu u00u1 u #d
100 1 $aBELLÉ, C.
245 $aHost susceptibility of hordeum vulgare to Meloidogyne graminicola.$h[electronic resource]
260 $c2020
500 $aArticle history: Accepted for publication:11/VII/2019 /Recibido: Aceptado para publicación: 2/IX/2020
520 $aAbstract: Barley (Hordeum vulgare) is an important crop used for human consumption and mainly by the malt industry. Recently, a species of the root-knot nematode (Meloidogyne graminicola) was found and recorded to parasitize barley roots in Brazil. In this context, the objective of this work was to evaluate the susceptibility of 17 barley genotypes to M. graminicola in the greenhouse. Barley genotypes were individually inoculated with 5,000 eggs + second stage juveniles (J2) of Meloidogyne and kept in a greenhouse. After 60 days, the roots of each plant were evaluated for gall number, number of nematodes per root gram and reproduction factor (RF = final population / initial population). All barley genotypes evaluated behaved as susceptible to M. graminicola. The cultivar 'Scarlett' had the lowest RF (14.9), while the 'BRS Itanema' resulted in the highest RF (24.5). Resumo: A cevada (Hordeum vulgare) é uma importante cultura utilizada na alimentação humana e principalmente pela indústria de maltes. Recentemente, uma espécie do nematoide-das-galhas (Meloidogyne graminicola) foi encontrada e registrada parasitando raízes de plantas de cevada no Brasil.Nesse contexto, o objetivo do trabalho foi avaliar, em casa de vegetação, a suscetibilidade de 17 cultivares de cevada a M. graminicola. As cultivares de cevada foram individualmente inoculadas com 5.000 ovos +juvenis do segundo estádio (J2) de Meloidogyne mantidas em casa de vegetação. Após 60 dias, as raízes de cada planta foram avaliadas quanto ao número de galhas, população final e fator de reprodução (FR=população final/população inicial). Todas as cultivares de cevada avaliadas comportaram-se como suscetíveis a M. graminicola. A cultivar ?Scarlett? apresentou o menor FR (14,9), enquanto que a ?BRS Itanema? resultou no maior FR (24,5).
653 $aBARLEY
653 $aREPRODUCTION
653 $aROOT-KNOT NEMATODE
653 $aSUSCEPTIBILITY
700 1 $aRAMOS, R.F.
700 1 $aKASPARY, T. E.
700 1 $aDOSSIN, M.F.
700 1 $aBRIDA, A.L.
700 1 $aANTONIOLLI, Z.I.
773 $tNematropica, 2020, vol. 50, p.96-100.
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
20/07/2022 |
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Actualizado : |
20/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. |
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Afiliación : |
MARTINA CRISPO, Laboratory Animal Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.; VANESSA CHENOUARD, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; PEDRO DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; LAURENT TESSON, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; JEAN-MARIE HESLAN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; IGNACIO ANEGÓN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. |
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Complemento del título : |
Chapter 12. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12 |
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DOI : |
10.1007/978-1-0716-2301-5_12 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations. |
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Palabras claves : |
Embryo; Genome editing; In vitro fertilization; OTOF; SsODNs. |
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Thesagro : |
OVEJA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 01915naa a2200301 a 4500
001 1063442
005 2022-07-20
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_12$2DOI
100 1 $aCRISPO, M.
245 $aGeneration of a Human Deafness Sheep Model Using the CRISPR/Cas System.$h[electronic resource]
260 $c2022
520 $aAbstract: CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations.
650 $aOVEJA
653 $aEmbryo
653 $aGenome editing
653 $aIn vitro fertilization
653 $aOTOF
653 $aSsODNs
700 1 $aCHENOUARD, V.
700 1 $aDOS SANTOS-NETO P
700 1 $aTESSON, L.
700 1 $aSOUZA-NEVES, M.
700 1 $aHESLAN, J.M.
700 1 $aCUADRO, F.
700 1 $aANEGÓN, I.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12
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