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| Registros recuperados : 11 | |
| 2. |  | GILSANZ, J.; ACOSTA, P.; ARANDA, S.; BANCHERO, L. Ensayo manejo de suelo. Jornada de divulgación, INIA Las Brujas, 21 noviembre 2013. In: INIA Las Brujas, Programa Nacional Producción Hortícola. Cultivo del chícharo. Jornada de divulgación. Canelones (UY): INIA, 2013. p. 1-6 (INIA Serie Actividades de Difusión; 726)| Biblioteca(s): INIA Las Brujas. |
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| 4. | | GONZÁLEZ, P.; BAO, L.; GALVÁN, G.; GRILLE, G.; MAESO, D.; CAMPELO, E.; BANCHERO, L.; CARREGA, E. Normas para la Producción Integrada de cebolla, zona Sur. Actualización 2018. Montevideo (UY): DIGEGRA-Facultad de Agronomía-INIA, 2018. 30 p. Actualización 2018 realizada sobre la norma original y sus respectivas actualizaciones.| Biblioteca(s): INIA Las Brujas. |
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| 5. | | GONZÁLEZ, P.; BAO, L.; GALVÁN, G.; GRILLE, G.; MAESO, D.; CAMPELO, E.; BANCHERO, L.; CARREGA, E. Normas para la Producción Integrada de morrón bajo invernáculo, zona Sur. Actualización 2018. Montevideo (UY): DIGEGRA-Facultad de Agronomía-INIA, 2018. 21 p. Actualización 2018, realizada sobre la norma original y sus respectivas actualizaciones.| Biblioteca(s): INIA Las Brujas. |
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| 6. | | GONZÁLEZ, P.; BAO, L.; GALVÁN, G.; GRILLE, G.; MAESO, D.; CAMPELO, E.; BANCHERO, L.; CARREGA, E. Normas para la Producción Integrada de tomate bajo invernáculo, zona Sur. Actualización 2018. Montevideo (UY): DIGEGRA-Facultad de Agronomía-INIA, 2018. 24 p. Actualización 2018, realizada sobre la norma original y sus respectivas actualizaciones.| Biblioteca(s): INIA Las Brujas. |
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| 7. | | BAO, L.; LORENZO, M. E.; GRILLÉ, G.; BANCHERO, L.; CAMPELO, E.; FASIOLO, C.; GALVÁN, G.; GONZÁLEZ, P.; RIGHI, E. Enemigos naturales de las principales plagas del cultivo de tomate. Proyecto FPTA 344; Proyecto GCP/URU/031/GFF. Montevideo (UY): UdelaR-Fac.Agronomía, INIA, MGPA-DIGEGRA, 2019. 12 p. Proyecto FPTA 344: Producción e Introducción de Agentes de Control Biológico en el manejo regional integrado de plagas en hortalizas.
Proyecto Plaguicidas-GCP/URU/031/GFF: Fortalecimiento de las capacidades para la gestión ambientalmente...| Biblioteca(s): INIA Las Brujas. |
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| 8. | | CAMPELO, E.; MAESO, D.; GIMENEZ, G.; BANCHERO, L.; ARBOLEYA, J.; MARTÍNEZ, D.; DOGLIOTTI, S.; VIETA, A.; PAULLIER, J. (Ed.). Producción integrada de tomate para industria. Montevideo (Uruguay): INIA, 2015. 58 p. (Boletín de Divulgación; 110)| Biblioteca(s): INIA Las Brujas; INIA Treinta y Tres. |
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| 9. | | CAMPELO, E.; BANCHERO, L.; VIETA, A.; GODÍN, A.; IURATO, A.; PEIRANO, Z.; CURBELO, Y.; GALVÁN, G.; BAO, L.; GONZÁLEZ, P.; FASIOLO, C. Control biológico de plagas y enfermedades en horticultura. Revista INIA Uruguay, 2019, no. 56, p. 84-87. (Revista INIA; 56)| Biblioteca(s): INIA Las Brujas; INIA Treinta y Tres. |
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| 10. | | VIETA, A.; ORIHUELA, C.; FASIOLO, C.; BAO, L.; GALVÁN, G.; BANCHERO, L.; IURATO, A.; GODÍN, A.; PRIMO, P.; WATERSTON, S.; MARTÍNEZ, F.; VIANA, V. Introducción de agentes de control biológico en el manejo integrado de plagas en horticultura. O17. Módulo 5: Desarrollos y perspectivas de empresas productoras y usuarias de bioinsumos. In: Abreo, E.; Beyhaut, E.; Rivas, F. (Eds.). Simposio Microorganismos para la Agricultura, 2. [Resúmenes y Posters]. Canelones (UY): INIA, 2022. p.37. (Serie Actividades de Difusión; 801).| Biblioteca(s): INIA Las Brujas. |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
09/04/2025 |
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Actualizado : |
09/04/2025 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SCHVARTZMAN, C.; MURCHIO, S.; CASTRO, A.; CIBILS-STEWART, X.; MIRANDA, P.; MUJICA, V.; PARDO, H.; DALLA RIZZA, M. |
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Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANALÍA CASTRO, Centro NanoMat, DETEMA, Instituto Polo Tecnológico de Pando, Facultad de Química, Universidad de la República, Pando, Uruguay; XIMENA CIBILS-STEWART, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0003-0296-5554; PABLO MIRANDA, Centro NanoMat, DETEMA, Instituto Polo Tecnológico de Pando, Facultad de Química, Universidad de la República, Pando, Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-9820-2879; HELENA PARDO, Centro NanoMat, DETEMA, Instituto Polo Tecnológico de Pando, Facultad de Química, Universidad de la República, Pando, Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0003-4065-0940. |
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Título : |
Chitosan double-stranded RNA nanocomplexes for Piezodorus guildinii control. [Research article]. |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
Pest Management Science, 2025. [Early view]. https://doi.org/10.1002/ps.8744 |
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ISSN : |
1526-498X |
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DOI : |
10.1002/ps.8744 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 September 2024, Revised 7 January 2025, First published 05 March 2025. -- Correspondence: Schvartzman, C.; email:schvartzman@inia.org.uy -- Funding: This work was funded by the FSA_1_2018_1_151855 Innovagro grant from Agencia Nacional de Investigación e Innovación
(ANII), and by the Instituto Nacional de Investivgación Agropecuaria (INIA) BT_20 grant. -- Publisher: John Wiley and Sons Ltd. -- |
|
Contenido : |
ABSTRACT.- BACKGROUND: Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated by double-stranded RNA (dsRNA), may be used as a safe technique in integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect dsRNA from degradation has shown to enhance this response. This study aims to assess the impact of dsRNA administration on the viability of P. guildinii and evaluate dsRNA-chitosan nanocomplexes as a pest control strategy. RESULTS: Ten target genes were selected, dsRNAs were designed and synthesized. In injection assays, significant mortality exceeding 76% was observed, while mortality from ingestion also showed significant differences, reaching 49%. The dsRNA targeting Srp54k gene was selected and a chitosan-dsRNA nanocomplex was synthesized. These nanocomplexes demonstrated enhanced stability under RNase treatment in vitro, and, in in vivo assays, a cumulative corrected mortality rate of 66% was reported. Consistent with these findings, RT-qPCR analysis revealed that gene expression levels in treated insects were significantly reduced compared to control at 24, 48, and 72 h post treatment for targeted genes. CONCLUSION: In this work, we evaluated the effect of dsRNA administration by injection and ingestion on the viability of P. guildinii and determined its molecular silencing effects. Additionally, we synthesized and characterized chitosan-dsRNA nanoparticles, which demonstrated improved efficacy in terms of stability. These findings are promising for the development of new integrated management strategies for stink bugs control. © 2025 Society of Chemical Industry. © 2025 Society of Chemical Industry. MenosABSTRACT.- BACKGROUND: Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated by double-stranded RNA (dsRNA), may be used as a safe technique in integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect dsRNA from degradation has shown to enhance this response. This study aims to assess the impact of dsRNA administration on the viability of P. guildinii and evaluate dsRNA-chitosan nanocomplexes as a pest control strategy. RESULTS: Ten target genes were selected, dsRNAs were designed and synthesized. In injection assays, significant mortality exceeding 76% was observed, while mortality from ingestion also showed significant differences, reaching 49%. The dsRNA targeting Srp54k gene was selected and a chitosan-dsRNA nanocomplex was synthesized. These nanocomplexes demonstrated enhanced stability under RNase treatment in vitro, and, in in vivo assays, a cumulative corrected mortality rate of 66% was reported. Consistent with these findings, RT-qPCR analysis revealed that gene expression levels in treated insects were significantly reduced compared to control at 24, 48, and 72 h post treatment for targeted genes. CONCLUSION: In this work, we evaluated the effect of dsRNA administration by injection and ingestion on the viability of P. guildinii a... Presentar Todo |
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Palabras claves : |
ÁREA DE MEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA; Gene silencing; Hemiptera; Pest control; RNAi; Stink bugs; Sustainable Development Goals (SDGs); Zero hunger - Goal 2. |
|
Asunto categoría : |
H10 Plagas de las plantas |
|
Marc : |
LEADER 03306naa a2200337 a 4500
001 1065164
005 2025-04-09
008 2025 bl uuuu u00u1 u #d
022 $a1526-498X
024 7 $a10.1002/ps.8744$2DOI
100 1 $aSCHVARTZMAN, C.
245 $aChitosan double-stranded RNA nanocomplexes for Piezodorus guildinii control. [Research article].$h[electronic resource]
260 $c2025
500 $aArticle history: Received 30 September 2024, Revised 7 January 2025, First published 05 March 2025. -- Correspondence: Schvartzman, C.; email:schvartzman@inia.org.uy -- Funding: This work was funded by the FSA_1_2018_1_151855 Innovagro grant from Agencia Nacional de Investigación e Innovación (ANII), and by the Instituto Nacional de Investivgación Agropecuaria (INIA) BT_20 grant. -- Publisher: John Wiley and Sons Ltd. --
520 $aABSTRACT.- BACKGROUND: Piezodorus guildinii (Westwood, Hemiptera: Pentatomidae) causes economic losses in soybean crops by diminishing productivity, impacting grain size, oil content, and germination power. RNA interference (RNAi), a conserved gene regulation process initiated by double-stranded RNA (dsRNA), may be used as a safe technique in integrated pest management strategies. Conjugating dsRNA with cationic polymers, which protect dsRNA from degradation has shown to enhance this response. This study aims to assess the impact of dsRNA administration on the viability of P. guildinii and evaluate dsRNA-chitosan nanocomplexes as a pest control strategy. RESULTS: Ten target genes were selected, dsRNAs were designed and synthesized. In injection assays, significant mortality exceeding 76% was observed, while mortality from ingestion also showed significant differences, reaching 49%. The dsRNA targeting Srp54k gene was selected and a chitosan-dsRNA nanocomplex was synthesized. These nanocomplexes demonstrated enhanced stability under RNase treatment in vitro, and, in in vivo assays, a cumulative corrected mortality rate of 66% was reported. Consistent with these findings, RT-qPCR analysis revealed that gene expression levels in treated insects were significantly reduced compared to control at 24, 48, and 72 h post treatment for targeted genes. CONCLUSION: In this work, we evaluated the effect of dsRNA administration by injection and ingestion on the viability of P. guildinii and determined its molecular silencing effects. Additionally, we synthesized and characterized chitosan-dsRNA nanoparticles, which demonstrated improved efficacy in terms of stability. These findings are promising for the development of new integrated management strategies for stink bugs control. © 2025 Society of Chemical Industry. © 2025 Society of Chemical Industry.
653 $aÁREA DE MEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA
653 $aGene silencing
653 $aHemiptera
653 $aPest control
653 $aRNAi
653 $aStink bugs
653 $aSustainable Development Goals (SDGs)
653 $aZero hunger - Goal 2
700 1 $aMURCHIO, S.
700 1 $aCASTRO, A.
700 1 $aCIBILS-STEWART, X.
700 1 $aMIRANDA, P.
700 1 $aMUJICA, V.
700 1 $aPARDO, H.
700 1 $aDALLA RIZZA, M.
773 $tPest Management Science, 2025. [Early view]. https://doi.org/10.1002/ps.8744
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