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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
10/09/2025 |
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Actualizado : |
10/09/2025 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FERNANDES, P. B. B.; FONSECA, M. E. N.; GONZÁLEZ-ARCOS, M.; BOITEUX, L. S.; ARAGÃO, F. J. L. |
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Afiliación : |
PEDRO B. B. FERNANDES, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil; Departamento de Biologia Celular, Universidade de Brasília, Campus Universitário, DF, Brasília, 70910-900, Brazil; MARIA E. N. FONSECA, Embrapa Hortaliças, Rodovia BR-060, Km 09, Fazenda Tamanduá, DF, Brasília, 70275-970, Brazil; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONARDO S. BOITEUX, Embrapa Hortaliças, Rodovia BR-060, Km 09, Fazenda Tamanduá, DF, Brasília, 70275-970, Brazil; FRANCISCO J. L. ARAGÃO, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil; Departamento de Biologia Celular, Universidade de Brasília, Campus Universitário, DF, Brasília, 70910-900, Brazil. |
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Título : |
Remodeling aboveground tomato plant architecture via CRISPR/Cas9-mediated editing of a single Tiller Angle Control 1-like gene. |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
aBIOTECH, 2025. [In Press]. https://doi.org/10.1007/s42994-025-00220-9 -- |
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ISSN : |
2096-6326. |
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DOI : |
10.1007/s42994-025-00220-9 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 27 March 2025, Accepted 19 May 2025. -- Correspondence: F.J.L. Aragão, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil, Email: francisco.aragao@embrapa.br -- Funding: The authors gratefully acknowledge the financial support of Conselho Nacional de Desenvolvimento Cientıíico e Tecnológico (CNPq), Brazil (Grant No. 304001/2023-6). Pedro B.B. Fernandes was supported by a fellowship from CNPq (Grant No. 140365/2023-0). This research was also supported by grants, scholarships, and fellowships from Embrapa (MENF, LSB, and FJLA), FAP-DF (MENF), and CNPq (MENF, LSB, and FJLA). |
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Contenido : |
ABSTRACT.- Plant architecture is a major factor affecting crop management and yield. The erect leaf phenotype is a key trait for improving light capture, reducing water loss, optimizing space utilization, and facilitating the chemical and biological control of arthropods and pathogens, especially those infesting/infecting abaxial leaf surfaces. This phenotype has been associated with Tiller Angle Control 1 (TAC1)-like genes across many herbaceous and tree species. Our previous genomic and genetic analyses of the erect leaf phenotype in tomato (Solanum lycopersicum) indicated that this trait is controlled by a semi-dominant locus, Erl, on chromosome 10. We discovered that this phenotype was in tight linkage with a candidate loss-of-function mutation in Solyc10g009320, an ortholog of TAC1-like genes. Therefore, editing this gene might confirm its function and enable the fine-tuned manipulation of aboveground tomato plant architecture. Here, we utilized a CRISPR/Cas9 genome editing system to confirm the complete genetic association of the erect leaf phenotype in tomato by knocking out Solyc10g009320 in tomato cultivar 'Micro-Tom'. In addition, we analyzed the effects of editing this gene on the overall plant phenotype as well as physiological and agronomic performance. Editing Solyc10g009320 alleles in tomato lays the foundation for the large-scale generation of superior genotypes, paving the way for the development of elite cultivars with an erect leaf phenotype.
© Agricultural Information Institute, Chinese Academy of Agricultural Sciences 2025. MenosABSTRACT.- Plant architecture is a major factor affecting crop management and yield. The erect leaf phenotype is a key trait for improving light capture, reducing water loss, optimizing space utilization, and facilitating the chemical and biological control of arthropods and pathogens, especially those infesting/infecting abaxial leaf surfaces. This phenotype has been associated with Tiller Angle Control 1 (TAC1)-like genes across many herbaceous and tree species. Our previous genomic and genetic analyses of the erect leaf phenotype in tomato (Solanum lycopersicum) indicated that this trait is controlled by a semi-dominant locus, Erl, on chromosome 10. We discovered that this phenotype was in tight linkage with a candidate loss-of-function mutation in Solyc10g009320, an ortholog of TAC1-like genes. Therefore, editing this gene might confirm its function and enable the fine-tuned manipulation of aboveground tomato plant architecture. Here, we utilized a CRISPR/Cas9 genome editing system to confirm the complete genetic association of the erect leaf phenotype in tomato by knocking out Solyc10g009320 in tomato cultivar 'Micro-Tom'. In addition, we analyzed the effects of editing this gene on the overall plant phenotype as well as physiological and agronomic performance. Editing Solyc10g009320 alleles in tomato lays the foundation for the large-scale generation of superior genotypes, paving the way for the development of elite cultivars with an erect leaf phenotype.
© Agricultur... Presentar Todo |
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Palabras claves : |
Dense cultivation; Erect leaf phenotype; Gene editing; Genetically modified plants; INIA SALTO GRANDE; SISTEMA VEGETAL INTENSIVO - INIA; Tomato; Transgenic plant. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 03189naa a2200301 a 4500
001 1065357
005 2025-09-10
008 2025 bl uuuu u00u1 u #d
022 $a2096-6326.
024 7 $a10.1007/s42994-025-00220-9$2DOI
100 1 $aFERNANDES, P. B. B.
245 $aRemodeling aboveground tomato plant architecture via CRISPR/Cas9-mediated editing of a single Tiller Angle Control 1-like gene.$h[electronic resource]
260 $c2025
500 $aArticle history: Received 27 March 2025, Accepted 19 May 2025. -- Correspondence: F.J.L. Aragão, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, DF, Brasília, 70770-900, Brazil, Email: francisco.aragao@embrapa.br -- Funding: The authors gratefully acknowledge the financial support of Conselho Nacional de Desenvolvimento Cientıíico e Tecnológico (CNPq), Brazil (Grant No. 304001/2023-6). Pedro B.B. Fernandes was supported by a fellowship from CNPq (Grant No. 140365/2023-0). This research was also supported by grants, scholarships, and fellowships from Embrapa (MENF, LSB, and FJLA), FAP-DF (MENF), and CNPq (MENF, LSB, and FJLA).
520 $aABSTRACT.- Plant architecture is a major factor affecting crop management and yield. The erect leaf phenotype is a key trait for improving light capture, reducing water loss, optimizing space utilization, and facilitating the chemical and biological control of arthropods and pathogens, especially those infesting/infecting abaxial leaf surfaces. This phenotype has been associated with Tiller Angle Control 1 (TAC1)-like genes across many herbaceous and tree species. Our previous genomic and genetic analyses of the erect leaf phenotype in tomato (Solanum lycopersicum) indicated that this trait is controlled by a semi-dominant locus, Erl, on chromosome 10. We discovered that this phenotype was in tight linkage with a candidate loss-of-function mutation in Solyc10g009320, an ortholog of TAC1-like genes. Therefore, editing this gene might confirm its function and enable the fine-tuned manipulation of aboveground tomato plant architecture. Here, we utilized a CRISPR/Cas9 genome editing system to confirm the complete genetic association of the erect leaf phenotype in tomato by knocking out Solyc10g009320 in tomato cultivar 'Micro-Tom'. In addition, we analyzed the effects of editing this gene on the overall plant phenotype as well as physiological and agronomic performance. Editing Solyc10g009320 alleles in tomato lays the foundation for the large-scale generation of superior genotypes, paving the way for the development of elite cultivars with an erect leaf phenotype. © Agricultural Information Institute, Chinese Academy of Agricultural Sciences 2025.
653 $aDense cultivation
653 $aErect leaf phenotype
653 $aGene editing
653 $aGenetically modified plants
653 $aINIA SALTO GRANDE
653 $aSISTEMA VEGETAL INTENSIVO - INIA
653 $aTomato
653 $aTransgenic plant
700 1 $aFONSECA, M. E. N.
700 1 $aGONZÁLEZ-ARCOS, M.
700 1 $aBOITEUX, L. S.
700 1 $aARAGÃO, F. J. L.
773 $taBIOTECH, 2025. [In Press]. https://doi.org/10.1007/s42994-025-00220-9 --
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